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The map label for this gene is ptsI [H]

Identifier: 218930043

GI number: 218930043

Start: 3343678

End: 3345405

Strand: Direct

Name: ptsI [H]

Synonym: YPO2994

Alternate gene names: 218930043

Gene position: 3343678-3345405 (Clockwise)

Preceding gene: 218930042

Following gene: 218930044

Centisome position: 71.85

GC content: 46.93

Gene sequence:

>1728_bases
ATGATTTCAGGCATTTTAGTATCACCGGGTATCGCTTTTGGTAAAGCACTTCTACTGAAAGAAGATGAGATTGTCATCAA
CCGGAAAAAGATCTCTGCTGACCAAGTGGAGCAGGAAGTCGAGCGTTTCAAAGCTGGGCGCGCCAAAGCGGCTGAACAGT
TAGAAGCGATTAAGACCAAAGCTGGCGTAAGCCTAGGCGAAGAGAAAGCTGCCATCTTCGAAGGGCATATCATGCTGTTG
GAAGACGAAGAGCTTGAGCAGGAAATCATAGCCCTCATCAAAGATGAGCATGCCTCTGCTGATGCAGCCGCTTATTCTGT
CATTGAAGGCCAGGCAAAAGCCCTGGAAGAATTAGATGACGAATACCTAAAAGAACGCGCTGCTGACGTACGTGACATCG
GTAAACGCCTGCTGAAAAATATTCTCGGTCTGAACATTGTTGATCTGAGTGCGATTCAGGATGAAGTTATTCTGGTTGCT
ACCGATTTGACGCCGTCAGAAACCGCACAGCTTAATTTGGATAAAGTGTTAGGGTTCATCACCGATATTGGTGGCCGTAC
CTCTCACACCTCAATTATGGCTCGCTCTCTTGAATTACCTGCCATCGTTGGTACCAGTAATGTGACCAAGCAGGTCAAGA
ATGATGATTACTTGATCCTCGATGCAGTTAACAACAAAGTTTATTTGAATCCAACTGCCGATGTGATCGAGCAGTTAAAA
GCGGTGAAAAACCAGTACATCACCGAAAAAAATGAACTGGCTAAATTGAAAGACCTGCCAGCTATCACCCTTGATGGTCA
TCAGGTTGAAGTGGTTGCCAACATTGGTACAGTGCGTGATATCGCCGGTGCTGAGCGTAATGGCGCTGAAGGTGTTGGTC
TGTATCGTACCGAATTCCTGTTCATGGACCGCGACTCTCTGCCTACAGAAGAAGAGCAGTTCCAGGCTTATAAAGCGGTA
GCAGAGGCCATGGGTTCTCAGGCAGTTATCGTCCGTACAATGGACATCGGTGGTGACAAAGATCTGCCGTATATGAACTT
GCCGAAAGAAGAAAACCCATTCCTGGGCTGGCGTGCTATTCGTATCGCTATGGATCGCAAAGAAATTCTGCACGCACAGC
TGCGGGCAATCTTGCGAGCGTCGGCGTTTGGTAAGTTGCGTATCATGTTCCCGATGATTATTTCGGTCGAAGAAGTACGT
GAACTGAAAGCAGAACTTGAACTGCTGAAAAGCCAGCTACGTGAAGAAAATAAGGCCTTTGATGAAACTATCGAGGTCGG
TGTAATGGTTGAAACACCTGCGGCGGCGGTTATCGCTCGCCATTTAGCTAAAGAAGTTGACTTCTTTAGTATTGGGACAA
ACGATCTAACTCAGTATACTCTGGCAGTAGATCGTGGCAATGAGCTGATTTCTCATCTCTATAATCCGATGTCGCCATCG
GTATTGGGCCTGATCAAACAGGTTATTGATGCATCTCACGCTGAAGGTAAGTGGACCGGTATGTGCGGTGAACTTGCTGG
CGATGAACGTGCTACACTGTTGCTATTGGGCATGGGGCTGGATGAATTCAGCATGAGTGCAATTTCGATCCCACGCATCA
AGAAAATTATCCGTAATACGAATTTCGAAGATGTGAAGGTGTTGGCAGAGCAGGCACTGGCTCAGCCAACAGCGAAGGAA
TTGATGGATTTGGTTACCACATTCATCGAAGAGAAAACGCTCTGCTAA

Upstream 100 bases:

>100_bases
TGAGAAAAAAGCGGTTGAGCATCTGGTAAAACTGATGGCAGAGCTTGAGTAATCAACGCGCTCTTTTTTAGTTAACACCA
GTCAAGAGTAAGGTAGGGTT

Downstream 100 bases:

>100_bases
TTCCACGATACTGGGACGCTGCCCAATATAAATACTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCATTGGT
TTCAGATGATAAAAAAGACA

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MISGILVSPGIAFGKALLLKEDEIVINRKKISADQVEQEVERFKAGRAKAAEQLEAIKTKAGVSLGEEKAAIFEGHIMLL
EDEELEQEIIALIKDEHASADAAAYSVIEGQAKALEELDDEYLKERAADVRDIGKRLLKNILGLNIVDLSAIQDEVILVA
TDLTPSETAQLNLDKVLGFITDIGGRTSHTSIMARSLELPAIVGTSNVTKQVKNDDYLILDAVNNKVYLNPTADVIEQLK
AVKNQYITEKNELAKLKDLPAITLDGHQVEVVANIGTVRDIAGAERNGAEGVGLYRTEFLFMDRDSLPTEEEQFQAYKAV
AEAMGSQAVIVRTMDIGGDKDLPYMNLPKEENPFLGWRAIRIAMDRKEILHAQLRAILRASAFGKLRIMFPMIISVEEVR
ELKAELELLKSQLREENKAFDETIEVGVMVETPAAAVIARHLAKEVDFFSIGTNDLTQYTLAVDRGNELISHLYNPMSPS
VLGLIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDVKVLAEQALAQPTAKE
LMDLVTTFIEEKTLC

Sequences:

>Translated_575_residues
MISGILVSPGIAFGKALLLKEDEIVINRKKISADQVEQEVERFKAGRAKAAEQLEAIKTKAGVSLGEEKAAIFEGHIMLL
EDEELEQEIIALIKDEHASADAAAYSVIEGQAKALEELDDEYLKERAADVRDIGKRLLKNILGLNIVDLSAIQDEVILVA
TDLTPSETAQLNLDKVLGFITDIGGRTSHTSIMARSLELPAIVGTSNVTKQVKNDDYLILDAVNNKVYLNPTADVIEQLK
AVKNQYITEKNELAKLKDLPAITLDGHQVEVVANIGTVRDIAGAERNGAEGVGLYRTEFLFMDRDSLPTEEEQFQAYKAV
AEAMGSQAVIVRTMDIGGDKDLPYMNLPKEENPFLGWRAIRIAMDRKEILHAQLRAILRASAFGKLRIMFPMIISVEEVR
ELKAELELLKSQLREENKAFDETIEVGVMVETPAAAVIARHLAKEVDFFSIGTNDLTQYTLAVDRGNELISHLYNPMSPS
VLGLIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDVKVLAEQALAQPTAKE
LMDLVTTFIEEKTLC
>Mature_575_residues
MISGILVSPGIAFGKALLLKEDEIVINRKKISADQVEQEVERFKAGRAKAAEQLEAIKTKAGVSLGEEKAAIFEGHIMLL
EDEELEQEIIALIKDEHASADAAAYSVIEGQAKALEELDDEYLKERAADVRDIGKRLLKNILGLNIVDLSAIQDEVILVA
TDLTPSETAQLNLDKVLGFITDIGGRTSHTSIMARSLELPAIVGTSNVTKQVKNDDYLILDAVNNKVYLNPTADVIEQLK
AVKNQYITEKNELAKLKDLPAITLDGHQVEVVANIGTVRDIAGAERNGAEGVGLYRTEFLFMDRDSLPTEEEQFQAYKAV
AEAMGSQAVIVRTMDIGGDKDLPYMNLPKEENPFLGWRAIRIAMDRKEILHAQLRAILRASAFGKLRIMFPMIISVEEVR
ELKAELELLKSQLREENKAFDETIEVGVMVETPAAAVIARHLAKEVDFFSIGTNDLTQYTLAVDRGNELISHLYNPMSPS
VLGLIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDVKVLAEQALAQPTAKE
LMDLVTTFIEEKTLC

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=575, Percent_Identity=86.7826086956522, Blast_Score=998, Evalue=0.0,
Organism=Escherichia coli, GI1788726, Length=574, Percent_Identity=39.198606271777, Blast_Score=387, Evalue=1e-108,
Organism=Escherichia coli, GI48994992, Length=510, Percent_Identity=39.0196078431373, Blast_Score=330, Evalue=1e-91,
Organism=Escherichia coli, GI1789193, Length=541, Percent_Identity=34.1959334565619, Blast_Score=311, Evalue=1e-85,
Organism=Escherichia coli, GI1787994, Length=449, Percent_Identity=25.8351893095768, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI226510935, Length=208, Percent_Identity=30.2884615384615, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 63379; Mature: 63379

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISGILVSPGIAFGKALLLKEDEIVINRKKISADQVEQEVERFKAGRAKAAEQLEAIKTK
CCCCEEECCCHHHHHHHEEECCCEEEEEHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
AGVSLGEEKAAIFEGHIMLLEDEELEQEIIALIKDEHASADAAAYSVIEGQAKALEELDD
HCCCCCCCCCEEEECEEEEEECHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
EYLKERAADVRDIGKRLLKNILGLNIVDLSAIQDEVILVATDLTPSETAQLNLDKVLGFI
HHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHCCCCEEEEEECCCCCCHHHCCHHHHHHHH
TDIGGRTSHTSIMARSLELPAIVGTSNVTKQVKNDDYLILDAVNNKVYLNPTADVIEQLK
HHCCCCCCHHHHHHHHCCCCEEECCCHHHHHHCCCCEEEEEECCCEEEECCHHHHHHHHH
AVKNQYITEKNELAKLKDLPAITLDGHQVEVVANIGTVRDIAGAERNGAEGVGLYRTEFL
HHHHHHCCCHHHHHHHHCCCEEEECCCEEEEEEECCCHHHHCCCCCCCCCCCCEEEEEEE
FMDRDSLPTEEEQFQAYKAVAEAMGSQAVIVRTMDIGGDKDLPYMNLPKEENPFLGWRAI
EEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEE
RIAMDRKEILHAQLRAILRASAFGKLRIMFPMIISVEEVRELKAELELLKSQLREENKAF
EEHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DETIEVGVMVETPAAAVIARHLAKEVDFFSIGTNDLTQYTLAVDRGNELISHLYNPMSPS
HHHHEEEEEEECCHHHHHHHHHHHHCCEEECCCCCCEEEEEEECCHHHHHHHHCCCCCHH
VLGLIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSAISIPRIKKIIRNT
HHHHHHHHHCCCCCCCCCCCCCHHCCCCCCEEEEEEECCCCHHHHHHHCHHHHHHHHHCC
NFEDVKVLAEQALAQPTAKELMDLVTTFIEEKTLC
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MISGILVSPGIAFGKALLLKEDEIVINRKKISADQVEQEVERFKAGRAKAAEQLEAIKTK
CCCCEEECCCHHHHHHHEEECCCEEEEEHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
AGVSLGEEKAAIFEGHIMLLEDEELEQEIIALIKDEHASADAAAYSVIEGQAKALEELDD
HCCCCCCCCCEEEECEEEEEECHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
EYLKERAADVRDIGKRLLKNILGLNIVDLSAIQDEVILVATDLTPSETAQLNLDKVLGFI
HHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHCCCCEEEEEECCCCCCHHHCCHHHHHHHH
TDIGGRTSHTSIMARSLELPAIVGTSNVTKQVKNDDYLILDAVNNKVYLNPTADVIEQLK
HHCCCCCCHHHHHHHHCCCCEEECCCHHHHHHCCCCEEEEEECCCEEEECCHHHHHHHHH
AVKNQYITEKNELAKLKDLPAITLDGHQVEVVANIGTVRDIAGAERNGAEGVGLYRTEFL
HHHHHHCCCHHHHHHHHCCCEEEECCCEEEEEEECCCHHHHCCCCCCCCCCCCEEEEEEE
FMDRDSLPTEEEQFQAYKAVAEAMGSQAVIVRTMDIGGDKDLPYMNLPKEENPFLGWRAI
EEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEE
RIAMDRKEILHAQLRAILRASAFGKLRIMFPMIISVEEVRELKAELELLKSQLREENKAF
EEHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DETIEVGVMVETPAAAVIARHLAKEVDFFSIGTNDLTQYTLAVDRGNELISHLYNPMSPS
HHHHEEEEEEECCHHHHHHHHHHHHCCEEECCCCCCEEEEEEECCHHHHHHHHCCCCCHH
VLGLIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSAISIPRIKKIIRNT
HHHHHHHHHCCCCCCCCCCCCCHHCCCCCCEEEEEEECCCCHHHHHHHCHHHHHHHHHCC
NFEDVKVLAEQALAQPTAKELMDLVTTFIEEKTLC
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2457575; 2960675; 9205837; 9278503; 2411636; 3290198; 9298646; 7876255; 8805571; 9054557; 9541412; 10048929 [H]