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The map label for this gene is cysK [H]

Identifier: 218930041

GI number: 218930041

Start: 3341905

End: 3342873

Strand: Direct

Name: cysK [H]

Synonym: YPO2992

Alternate gene names: 218930041

Gene position: 3341905-3342873 (Clockwise)

Preceding gene: 218930040

Following gene: 218930042

Centisome position: 71.81

GC content: 49.95

Gene sequence:

>969_bases
ATGAGCAAGATATATGAAGACAACTCTTTAACAATCGGCCATACGCCGCTGGTTCGTCTGAACCGTATCGGCAACGGACG
TATTTTGGCGAAAGTTGAATCGCGTAACCCAAGTTTCAGTGTGAAGTGCCGTATCGGCGCCAACATGATTTGGGATGCTG
AAAAACGGGGTATTCTGACCGCAGGCAAAGAGCTGGTTGAACCAACCAGCGGGAATACAGGTGTTGCCCTGGCATTTGTC
GCCGCTGCACGTGGCTATAAATTAACCCTTACCATGCCTGAAACTATGAGCATTGAGCGCCGCAAGTTGCTGAAAGCACT
GGGGGCAAATTTGGTGCTTACCGAAGGTGCCAAAGGGATGAAAGGCGCGATTGCCAAAGCGGAAGAAATTCAAGCAACCG
ATCCAGAACGCTACCTTATCTTGCAACAATTCAGTAACCCGGCTAACCCTGAAATTCATGAAAAAACCACCGGCCCTGAA
ATTTGGGAAGATACCGATGGTGATGTAGATGTCTTTGTCTCCGGGGTAGGTACCGGTGGTACCCTAACCGGTGTCAGCCG
CTATATTAAAAACACCCAAGGCAAAGCCATCACGACTGTGGCCGTCGAACCCACTGACTCCCCCGTGATTACCCAAGCGC
TGGCCGGTCAAGAGATCAAACCTGGTCCGCATAAAATTCAAGGTATTGGTGCGGGTTTTATCCCCGGTAACCTCGACTTG
AGTCTGGTTGATCGTGTAGCGCTGGTCACCAATGAAGAAGCTATTTCGATGGCCCGCCGTTTGATGGATGAAGAAGGCAT
TCTGGCGGGTATTTCTTCAGGTGCAGCCGTTGCTGCCGCCGTTAAGCTTTCAGAAGAGGAAGGCTTTACTGACAAAACAA
TTGTGGTTATCCTTCCCTCCTCGGGTGAACGGTATTTAAGTACAGCATTGTTCGCAGATTTGTTCACAGAGCAAGAGCTG
CAACAGTAG

Upstream 100 bases:

>100_bases
TTAATGACATATCGATATACCAATTACTTACTTCCATAGCCATACTGTTCGCGTATGATTTGAACGTTCCCTACATTTTC
ATAGAGTTAAAGGACGGGCT

Downstream 100 bases:

>100_bases
TCTGGGCTACCGCACTAAATGCAAAAAAAGCACCCTTTGGGGTGCTTTTTTTGTAGAGTACATCAAAGTTTTTACTACAT
AAAGATTGATTTTACCCTCC

Product: cysteine synthase A

Products: NA

Alternate protein names: CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; Sulfate starvation-induced protein 5; SSI5 [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MSKIYEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCRIGANMIWDAEKRGILTAGKELVEPTSGNTGVALAFV
AAARGYKLTLTMPETMSIERRKLLKALGANLVLTEGAKGMKGAIAKAEEIQATDPERYLILQQFSNPANPEIHEKTTGPE
IWEDTDGDVDVFVSGVGTGGTLTGVSRYIKNTQGKAITTVAVEPTDSPVITQALAGQEIKPGPHKIQGIGAGFIPGNLDL
SLVDRVALVTNEEAISMARRLMDEEGILAGISSGAAVAAAVKLSEEEGFTDKTIVVILPSSGERYLSTALFADLFTEQEL
QQ

Sequences:

>Translated_322_residues
MSKIYEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCRIGANMIWDAEKRGILTAGKELVEPTSGNTGVALAFV
AAARGYKLTLTMPETMSIERRKLLKALGANLVLTEGAKGMKGAIAKAEEIQATDPERYLILQQFSNPANPEIHEKTTGPE
IWEDTDGDVDVFVSGVGTGGTLTGVSRYIKNTQGKAITTVAVEPTDSPVITQALAGQEIKPGPHKIQGIGAGFIPGNLDL
SLVDRVALVTNEEAISMARRLMDEEGILAGISSGAAVAAAVKLSEEEGFTDKTIVVILPSSGERYLSTALFADLFTEQEL
QQ
>Mature_321_residues
SKIYEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCRIGANMIWDAEKRGILTAGKELVEPTSGNTGVALAFVA
AARGYKLTLTMPETMSIERRKLLKALGANLVLTEGAKGMKGAIAKAEEIQATDPERYLILQQFSNPANPEIHEKTTGPEI
WEDTDGDVDVFVSGVGTGGTLTGVSRYIKNTQGKAITTVAVEPTDSPVITQALAGQEIKPGPHKIQGIGAGFIPGNLDLS
LVDRVALVTNEEAISMARRLMDEEGILAGISSGAAVAAAVKLSEEEGFTDKTIVVILPSSGERYLSTALFADLFTEQELQ
Q

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=327, Percent_Identity=39.1437308868502, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI295821200, Length=327, Percent_Identity=39.1437308868502, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI4557415, Length=327, Percent_Identity=39.1437308868502, Blast_Score=200, Evalue=2e-51,
Organism=Escherichia coli, GI1788754, Length=323, Percent_Identity=87.6160990712074, Blast_Score=553, Evalue=1e-159,
Organism=Escherichia coli, GI2367138, Length=311, Percent_Identity=42.443729903537, Blast_Score=219, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI17535051, Length=307, Percent_Identity=53.0944625407166, Blast_Score=297, Evalue=5e-81,
Organism=Caenorhabditis elegans, GI17562970, Length=316, Percent_Identity=49.0506329113924, Blast_Score=278, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI32566674, Length=307, Percent_Identity=49.5114006514658, Blast_Score=268, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI115535073, Length=317, Percent_Identity=50.788643533123, Blast_Score=268, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI17561720, Length=316, Percent_Identity=49.6835443037975, Blast_Score=253, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI32566672, Length=226, Percent_Identity=47.787610619469, Blast_Score=204, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI17534315, Length=318, Percent_Identity=39.3081761006289, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI25147552, Length=327, Percent_Identity=36.3914373088685, Blast_Score=147, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17561716, Length=131, Percent_Identity=47.3282442748092, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321594, Length=313, Percent_Identity=36.741214057508, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6321449, Length=321, Percent_Identity=31.4641744548287, Blast_Score=121, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24643623, Length=314, Percent_Identity=37.2611464968153, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI20129101, Length=314, Percent_Identity=37.2611464968153, Blast_Score=159, Evalue=2e-39,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 34319; Mature: 34187

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIYEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCRIGANMIWDAEKRGILT
CCCEECCCCEEECCCCEEEEEECCCCEEEEEEECCCCCEEEEEEECCCEEECCCCCCEEC
AGKELVEPTSGNTGVALAFVAAARGYKLTLTMPETMSIERRKLLKALGANLVLTEGAKGM
CCHHHCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCC
KGAIAKAEEIQATDPERYLILQQFSNPANPEIHEKTTGPEIWEDTDGDVDVFVSGVGTGG
HHHHHCHHHCCCCCCHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
TLTGVSRYIKNTQGKAITTVAVEPTDSPVITQALAGQEIKPGPHKIQGIGAGFIPGNLDL
HHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCH
SLVDRVALVTNEEAISMARRLMDEEGILAGISSGAAVAAAVKLSEEEGFTDKTIVVILPS
HHHHEEHEEECHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEECCCCCCCCCEEEEEECC
SGERYLSTALFADLFTEQELQQ
CCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKIYEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCRIGANMIWDAEKRGILT
CCEECCCCEEECCCCEEEEEECCCCEEEEEEECCCCCEEEEEEECCCEEECCCCCCEEC
AGKELVEPTSGNTGVALAFVAAARGYKLTLTMPETMSIERRKLLKALGANLVLTEGAKGM
CCHHHCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCC
KGAIAKAEEIQATDPERYLILQQFSNPANPEIHEKTTGPEIWEDTDGDVDVFVSGVGTGG
HHHHHCHHHCCCCCCHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
TLTGVSRYIKNTQGKAITTVAVEPTDSPVITQALAGQEIKPGPHKIQGIGAGFIPGNLDL
HHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCH
SLVDRVALVTNEEAISMARRLMDEEGILAGISSGAAVAAAVKLSEEEGFTDKTIVVILPS
HHHHEEHEEECHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEECCCCCCCCCEEEEEECC
SGERYLSTALFADLFTEQELQQ
CCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]