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The map label for this gene is hisM [H]

Identifier: 218929848

GI number: 218929848

Start: 3110665

End: 3111381

Strand: Direct

Name: hisM [H]

Synonym: YPO2776

Alternate gene names: 218929848

Gene position: 3110665-3111381 (Clockwise)

Preceding gene: 218929847

Following gene: 218929849

Centisome position: 66.84

GC content: 45.89

Gene sequence:

>717_bases
ATGATTGAAATCTTACATCAATATTGGCAGGCATTACTTTGGAGTGATGGCTACCGCTTTACCGGCATGGCGGTCACTCT
CTGGTTATTAATTTCTTCGGTCGTCATGGGGGGGTTACTGGCGCTCCCATTGGCGGTTGCTCGGGTGTCTGAACGGAGAT
GGATCCGTTTACCAGTATGGATTTTTACCTATATATTCCGTGGTACACCGCTGTATGTGCAGCTATTGGTGTTCTATTCC
GGAATGTATAGTTTGGAGATAATCCGAGGCAATGATCTGCTCAATGCTTTTTTTCGCAGTGGATTAAATTGCACTATTTT
AGCGCTGACGCTTAATACCTGTGCTTATACCACTGAGATTTTTGCCGGTGCGATCCGTTCGGTACCTCATGGTGAAATAG
AAGCAGCACGCGCCTATGGTTTCTCACGTTTTAAATTATACCGCTGCATTATCTTACCTGCTGCATTGCGTATCTCTTTA
CCGGCATACAGCAATGAAGTGATCTTAATGCTGCACTCCACCGCGCTGGCTTTTACCGCGACCGTACCCGATATATTGAA
AATAGCTCGCGATATTAACGCGGCGACTTACCAACCCTTTTATGCGTTCAGTATTGCCGCTGTACTTTATTTGAGCATCT
CCTTTACGCTAATTAGCCTGTTCCGCCAGGCAGAAAAACGTTGGTTAGCCCATATTCAACCTCAGGCATCGCATTAG

Upstream 100 bases:

>100_bases
TGGTTGCTGGTGCGATTTATCTGTTCTTTACCACTCTCTCTAACGGTGTCTTGTGGTGGCTGGAAAGGCGCTATTCCCAT
GGGGTGAAGAGGGCTGAGCT

Downstream 100 bases:

>100_bases
TGCGGAGAACTTTATGACGAATACGTCCTATATTAAAAATGCGGAAACAAAACTTTCCGTGACGGAGCTGCATAAGCATT
ATGGGGAGCATGAGGTATTA

Product: histidine transport system permease HisM

Products: ADP; phosphate; L-histidine [Cytoplasm]; ADP; L-ornithine [Cytoplasm]; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MIEILHQYWQALLWSDGYRFTGMAVTLWLLISSVVMGGLLALPLAVARVSERRWIRLPVWIFTYIFRGTPLYVQLLVFYS
GMYSLEIIRGNDLLNAFFRSGLNCTILALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSRFKLYRCIILPAALRISL
PAYSNEVILMLHSTALAFTATVPDILKIARDINAATYQPFYAFSIAAVLYLSISFTLISLFRQAEKRWLAHIQPQASH

Sequences:

>Translated_238_residues
MIEILHQYWQALLWSDGYRFTGMAVTLWLLISSVVMGGLLALPLAVARVSERRWIRLPVWIFTYIFRGTPLYVQLLVFYS
GMYSLEIIRGNDLLNAFFRSGLNCTILALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSRFKLYRCIILPAALRISL
PAYSNEVILMLHSTALAFTATVPDILKIARDINAATYQPFYAFSIAAVLYLSISFTLISLFRQAEKRWLAHIQPQASH
>Mature_238_residues
MIEILHQYWQALLWSDGYRFTGMAVTLWLLISSVVMGGLLALPLAVARVSERRWIRLPVWIFTYIFRGTPLYVQLLVFYS
GMYSLEIIRGNDLLNAFFRSGLNCTILALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSRFKLYRCIILPAALRISL
PAYSNEVILMLHSTALAFTATVPDILKIARDINAATYQPFYAFSIAAVLYLSISFTLISLFRQAEKRWLAHIQPQASH

Specific function: Part of the binding-protein-dependent transport system for histidine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4160

COG function: function code E; ABC-type arginine/histidine transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788645, Length=238, Percent_Identity=81.5126050420168, Blast_Score=413, Evalue=1e-117,
Organism=Escherichia coli, GI1787086, Length=214, Percent_Identity=31.7757009345794, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1788646, Length=205, Percent_Identity=34.1463414634146, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1788226, Length=225, Percent_Identity=30.2222222222222, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1787030, Length=207, Percent_Identity=29.951690821256, Blast_Score=85, Evalue=3e-18,
Organism=Escherichia coli, GI1787087, Length=207, Percent_Identity=27.0531400966184, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786873, Length=190, Percent_Identity=27.8947368421053, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082239, Length=214, Percent_Identity=27.5700934579439, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786874, Length=203, Percent_Identity=27.0935960591133, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI48994930, Length=173, Percent_Identity=29.4797687861272, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 26954; Mature: 26954

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEILHQYWQALLWSDGYRFTGMAVTLWLLISSVVMGGLLALPLAVARVSERRWIRLPVW
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHH
IFTYIFRGTPLYVQLLVFYSGMYSLEIIRGNDLLNAFFRSGLNCTILALTLNTCAYTTEI
HHHHHHCCCHHHHHHHHHHCCCEEEEEEECCHHHHHHHHCCCCEEEEEEEHHHHHHHHHH
FAGAIRSVPHGEIEAARAYGFSRFKLYRCIILPAALRISLPAYSNEVILMLHSTALAFTA
HHHHHHCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHEEECCCCCCCEEEEEECHHHHHHH
TVPDILKIARDINAATYQPFYAFSIAAVLYLSISFTLISLFRQAEKRWLAHIQPQASH
HHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIEILHQYWQALLWSDGYRFTGMAVTLWLLISSVVMGGLLALPLAVARVSERRWIRLPVW
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHH
IFTYIFRGTPLYVQLLVFYSGMYSLEIIRGNDLLNAFFRSGLNCTILALTLNTCAYTTEI
HHHHHHCCCHHHHHHHHHHCCCEEEEEEECCHHHHHHHHCCCCEEEEEEEHHHHHHHHHH
FAGAIRSVPHGEIEAARAYGFSRFKLYRCIILPAALRISLPAYSNEVILMLHSTALAFTA
HHHHHHCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHEEECCCCCCCEEEEEECHHHHHHH
TVPDILKIARDINAATYQPFYAFSIAAVLYLSISFTLISLFRQAEKRWLAHIQPQASH
HHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-histidine [Periplasm]; H2O; ATP; L-ornithine [Periplasm]; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-histidine [Periplasm] + H2O = ADP + phosphate + L-histidine [Cytoplasm] ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [P

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]