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The map label for this gene is hisJ [H]

Identifier: 218929846

GI number: 218929846

Start: 3109091

End: 3109879

Strand: Direct

Name: hisJ [H]

Synonym: YPO2774

Alternate gene names: 218929846

Gene position: 3109091-3109879 (Clockwise)

Preceding gene: 218929845

Following gene: 218929847

Centisome position: 66.81

GC content: 43.85

Gene sequence:

>789_bases
ATGTTTATGAATAAAAAGCTGCGAGTTCTTCCTTTGGTATTCGCATTCGCTGCGGCTAGTAGTGCTTTTGCCGCCATTCC
AAAAGAGATCAAAATTGGCACAGATCCGACTTACGCCCCTTTTGAATCGAAAAACTCGAGTGGTGAATTAGTGGGTTTTG
ATATCGATATCGCGAAGGAGTTATGCAAACGTATAGATACCAAATGTACTTTTGTTGAAAGTGACTTTGATGCGCTAATC
CCGTCGCTGAAAGCGAAAAAAGTCGATGCCATTATCTCCTCTCTCTCCATTACTGAAAAACGCCAAAAAGAGATTGCCTT
CACCGATAAACTCTTTGCCGCCAATTCCCGTTTAATTGCTGCTAAAGGTAGCACTATTGAGCCGACACTGGTGGCATTAA
AAGGTAAGCGCGTGGGGGTGTTACAAGGGTCGACTCAGGAAGCTTTTGCCAATGCTGAGTGGCAGCCAAAAGGCATTGAT
GTTGTTGCTTATCAAAATCAGGATCTGATTTATGCAGATCTGGCCTCTGGCCGTATTGATGCGGCTTTCCAGGATGAAGT
GGCCGCCAGTGAAGGTTTCCTAAAACAACCTGTGGGTAAAGATTATATCTTTGCCGGTCCATCAGTAAAAAATGATCAAT
TCTTCGGTGTAGGGACTGGCATGGGGTTGCGCAAAGCCGATGTTGAGCTGAAAGAGGCGTTAAATAAAGCGTTTGCTGAA
ATGCGTAATGACGGCACCTACGATCAATTTGCCAAAAAATACTTCGATTTTGATGTTTACGGTGATTAA

Upstream 100 bases:

>100_bases
AGATAATTACTGGCACGATTACTGCAAATTGATTTATGGACGCTCAGGCTTGGACAGAAATCTAACGCGCTTTGTGGCAT
CAAAAATCAATTTGAGGGTT

Downstream 100 bases:

>100_bases
TCCTTTTTGTCGTCGTGGCCTCTGTCGATTGTGGCAGGGGCTAACATACCGTAATTAACCAGACTCCTCACTCAGTAAAA
CAGGATAACCCACAGATCAC

Product: histidine-binding periplasmic protein

Products: ADP; phosphate; L-histidine [Cytoplasm] [C]

Alternate protein names: HBP [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MFMNKKLRVLPLVFAFAAASSAFAAIPKEIKIGTDPTYAPFESKNSSGELVGFDIDIAKELCKRIDTKCTFVESDFDALI
PSLKAKKVDAIISSLSITEKRQKEIAFTDKLFAANSRLIAAKGSTIEPTLVALKGKRVGVLQGSTQEAFANAEWQPKGID
VVAYQNQDLIYADLASGRIDAAFQDEVAASEGFLKQPVGKDYIFAGPSVKNDQFFGVGTGMGLRKADVELKEALNKAFAE
MRNDGTYDQFAKKYFDFDVYGD

Sequences:

>Translated_262_residues
MFMNKKLRVLPLVFAFAAASSAFAAIPKEIKIGTDPTYAPFESKNSSGELVGFDIDIAKELCKRIDTKCTFVESDFDALI
PSLKAKKVDAIISSLSITEKRQKEIAFTDKLFAANSRLIAAKGSTIEPTLVALKGKRVGVLQGSTQEAFANAEWQPKGID
VVAYQNQDLIYADLASGRIDAAFQDEVAASEGFLKQPVGKDYIFAGPSVKNDQFFGVGTGMGLRKADVELKEALNKAFAE
MRNDGTYDQFAKKYFDFDVYGD
>Mature_262_residues
MFMNKKLRVLPLVFAFAAASSAFAAIPKEIKIGTDPTYAPFESKNSSGELVGFDIDIAKELCKRIDTKCTFVESDFDALI
PSLKAKKVDAIISSLSITEKRQKEIAFTDKLFAANSRLIAAKGSTIEPTLVALKGKRVGVLQGSTQEAFANAEWQPKGID
VVAYQNQDLIYADLASGRIDAAFQDEVAASEGFLKQPVGKDYIFAGPSVKNDQFFGVGTGMGLRKADVELKEALNKAFAE
MRNDGTYDQFAKKYFDFDVYGD

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788648, Length=236, Percent_Identity=78.8135593220339, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1788649, Length=260, Percent_Identity=68.8461538461538, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1787085, Length=245, Percent_Identity=39.1836734693878, Blast_Score=164, Evalue=6e-42,
Organism=Escherichia coli, GI1787088, Length=245, Percent_Identity=34.6938775510204, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI1788228, Length=230, Percent_Identity=32.1739130434783, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI1787031, Length=250, Percent_Identity=28.4, Blast_Score=100, Evalue=8e-23,

Paralogues:

None

Copy number: 3400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28634; Mature: 28634

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFMNKKLRVLPLVFAFAAASSAFAAIPKEIKIGTDPTYAPFESKNSSGELVGFDIDIAKE
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCEEEEEHHHHHH
LCKRIDTKCTFVESDFDALIPSLKAKKVDAIISSLSITEKRQKEIAFTDKLFAANSRLIA
HHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
AKGSTIEPTLVALKGKRVGVLQGSTQEAFANAEWQPKGIDVVAYQNQDLIYADLASGRID
ECCCCCCCEEEEECCCEEEEEECCCHHHHCCCCCCCCCEEEEEECCCCEEEEECCCCCCC
AAFQDEVAASEGFLKQPVGKDYIFAGPSVKNDQFFGVGTGMGLRKADVELKEALNKAFAE
CHHHHHHHHCCCHHHCCCCCCEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHH
MRNDGTYDQFAKKYFDFDVYGD
HCCCCCHHHHHHHHCCCEECCC
>Mature Secondary Structure
MFMNKKLRVLPLVFAFAAASSAFAAIPKEIKIGTDPTYAPFESKNSSGELVGFDIDIAKE
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCEEEEEHHHHHH
LCKRIDTKCTFVESDFDALIPSLKAKKVDAIISSLSITEKRQKEIAFTDKLFAANSRLIA
HHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
AKGSTIEPTLVALKGKRVGVLQGSTQEAFANAEWQPKGIDVVAYQNQDLIYADLASGRID
ECCCCCCCEEEEECCCEEEEEECCCHHHHCCCCCCCCCEEEEEECCCCEEEEECCCCCCC
AAFQDEVAASEGFLKQPVGKDYIFAGPSVKNDQFFGVGTGMGLRKADVELKEALNKAFAE
CHHHHHHHHCCCHHHCCCCCCEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHH
MRNDGTYDQFAKKYFDFDVYGD
HCCCCCHHHHHHHHCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-histidine [Periplasm]; H2O [C]

Specific reaction: ATP + L-histidine [Periplasm] + H2O = ADP + phosphate + L-histidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]