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The map label for this gene is folC [H]

Identifier: 218929841

GI number: 218929841

Start: 3103256

End: 3104560

Strand: Direct

Name: folC [H]

Synonym: YPO2769

Alternate gene names: 218929841

Gene position: 3103256-3104560 (Clockwise)

Preceding gene: 218929840

Following gene: 218929842

Centisome position: 66.68

GC content: 53.1

Gene sequence:

>1305_bases
ATGAACAATCATCAAACTCCCCAAGCCACGTCGCCTTTGGCCGCGTGGCTTTGCTATCTTGAGCATTTGCATTCTCAGCC
CATTGAGTTGGGTCTGGAACGTGTTAAACAGGTTGCTGAGCGTCTGGATCTGCTTAAACCTGCCCCCAAAATTTTTACCG
TTGCCGGTACCAACGGCAAAGGTACCACTTGTTGTACATTGGAAGCCATTTTATTGGCGGCCGGTCTGCGAGTCGGGGTC
TACAGCTCCCCCCACCTTTTACGCTATACCGAGCGGGTTCGAATTCAGGGGCAAGAGCTATCTGAGGCAGAACACAGCCA
CTCTTTTGCCCAGATCGAAGCCGGGCGAGGTGATATCTCACTGACCTACTTTGAATTTGGCACCTTATCAGCGTTGCAAC
TATTTAAGCAGGCTAAACTTGATGTGGTGATCCTGGAAGTCGGGTTGGGTGGGCGGTTAGATGCGACCAATATTGTTGAC
TCTGATGTCGCCGCCATCACCAGTATCGCGTTAGATCATACTGATTGGCTGGGTTATGACCGTGAAAGCATTGGCCGCGA
GAAGGCGGGTGTCTTCCGTGGCGGTAAACCTGCGGTCGTGGGTGAACCAGATATGCCCCAGTCTATTGCCGATGTGGCGG
CTGAGTTAGGCGCACAGCTCTATCGTCGGGATGTTGCCTGGAAATTCAGCCAGCAAGAACCGTTTGATCAACAAGAACCC
GTTGATCAACAAATAAACGGCTGGCACTGGCAGTGCGGTGAGCGCCAATTAACCGGCTTACCGGTGCCGAACGTGCCGCT
GGCGAATGCGGCTACGGCGCTGGCTGTACTGCATTATTCTGAGTTGCCATTGAGTGACGAGGCGATTCGGCAGGGGTTAC
AGGCCGCCAGTTTACCAGGGCGTTTTCAGGTCGTCAGTGAGCAACCGTTGCTGATTCTGGATGTCGCCCATAATCCGCAT
GCGGCCCGTTATCTGGTCAATCGGTTGGCCCAAGTTATTAACCCGGTTAATGCGTCGAAACAAGGCAAAGTGCGGGCAGT
CGTCGGGATGCTATCGGATAAAGATATTGCGGGTACGCTGGCATGTTTATCTGAGCGAGTTGATGAGTGGTATTGCGCTC
CGCTTGAAGGGCCACGAGGAGCCAGTGCTGGGCAATTGGCCGAACATCTGGTGAGCGCACGTCAGTTTAGTGATGTAGAA
ACTGCCTGGCGCCAGGCTATGCAAGATGCAGATACACAGGATGTGGTCATTGTCTGTGGCTCTTTCCATACTGTTGCCCA
TGTTATGGCAGCATTGCATTTGTGA

Upstream 100 bases:

>100_bases
GAGTCCCTAAGCATTTTCGATATTGGGAGGAACAACGGGAGCTAGCGTACAGGCAACTTGGAGTATCGCGGGGATAAAAC
CAAGGGAAGTGACGGGACTC

Downstream 100 bases:

>100_bases
CAGTGTCCAGTTTATAACAGATTGAATTTAAACCCAAAGAGCATGGAGTTGCAGGCGGGCAGTAAACGATCGCTTCCTGC
ATCTTCAAGTCCGGCGGGAG

Product: bifunctional folylpolyglutamate synthase/ dihydrofolate synthase

Products: NA

Alternate protein names: Folylpolyglutamate synthase; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; Dihydrofolate synthase [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MNNHQTPQATSPLAAWLCYLEHLHSQPIELGLERVKQVAERLDLLKPAPKIFTVAGTNGKGTTCCTLEAILLAAGLRVGV
YSSPHLLRYTERVRIQGQELSEAEHSHSFAQIEAGRGDISLTYFEFGTLSALQLFKQAKLDVVILEVGLGGRLDATNIVD
SDVAAITSIALDHTDWLGYDRESIGREKAGVFRGGKPAVVGEPDMPQSIADVAAELGAQLYRRDVAWKFSQQEPFDQQEP
VDQQINGWHWQCGERQLTGLPVPNVPLANAATALAVLHYSELPLSDEAIRQGLQAASLPGRFQVVSEQPLLILDVAHNPH
AARYLVNRLAQVINPVNASKQGKVRAVVGMLSDKDIAGTLACLSERVDEWYCAPLEGPRGASAGQLAEHLVSARQFSDVE
TAWRQAMQDADTQDVVIVCGSFHTVAHVMAALHL

Sequences:

>Translated_434_residues
MNNHQTPQATSPLAAWLCYLEHLHSQPIELGLERVKQVAERLDLLKPAPKIFTVAGTNGKGTTCCTLEAILLAAGLRVGV
YSSPHLLRYTERVRIQGQELSEAEHSHSFAQIEAGRGDISLTYFEFGTLSALQLFKQAKLDVVILEVGLGGRLDATNIVD
SDVAAITSIALDHTDWLGYDRESIGREKAGVFRGGKPAVVGEPDMPQSIADVAAELGAQLYRRDVAWKFSQQEPFDQQEP
VDQQINGWHWQCGERQLTGLPVPNVPLANAATALAVLHYSELPLSDEAIRQGLQAASLPGRFQVVSEQPLLILDVAHNPH
AARYLVNRLAQVINPVNASKQGKVRAVVGMLSDKDIAGTLACLSERVDEWYCAPLEGPRGASAGQLAEHLVSARQFSDVE
TAWRQAMQDADTQDVVIVCGSFHTVAHVMAALHL
>Mature_434_residues
MNNHQTPQATSPLAAWLCYLEHLHSQPIELGLERVKQVAERLDLLKPAPKIFTVAGTNGKGTTCCTLEAILLAAGLRVGV
YSSPHLLRYTERVRIQGQELSEAEHSHSFAQIEAGRGDISLTYFEFGTLSALQLFKQAKLDVVILEVGLGGRLDATNIVD
SDVAAITSIALDHTDWLGYDRESIGREKAGVFRGGKPAVVGEPDMPQSIADVAAELGAQLYRRDVAWKFSQQEPFDQQEP
VDQQINGWHWQCGERQLTGLPVPNVPLANAATALAVLHYSELPLSDEAIRQGLQAASLPGRFQVVSEQPLLILDVAHNPH
AARYLVNRLAQVINPVNASKQGKVRAVVGMLSDKDIAGTLACLSERVDEWYCAPLEGPRGASAGQLAEHLVSARQFSDVE
TAWRQAMQDADTQDVVIVCGSFHTVAHVMAALHL

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=375, Percent_Identity=31.4666666666667, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI66932984, Length=375, Percent_Identity=31.4666666666667, Blast_Score=132, Evalue=5e-31,
Organism=Escherichia coli, GI1788654, Length=425, Percent_Identity=65.8823529411765, Blast_Score=543, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI17553150, Length=310, Percent_Identity=31.9354838709677, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71984923, Length=310, Percent_Identity=31.9354838709677, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17553148, Length=310, Percent_Identity=31.9354838709677, Blast_Score=136, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6323760, Length=331, Percent_Identity=29.607250755287, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6324815, Length=276, Percent_Identity=29.7101449275362, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24641571, Length=177, Percent_Identity=38.4180790960452, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24581568, Length=203, Percent_Identity=34.4827586206897, Blast_Score=102, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17; =6.3.2.12 [H]

Molecular weight: Translated: 47229; Mature: 47229

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNHQTPQATSPLAAWLCYLEHLHSQPIELGLERVKQVAERLDLLKPAPKIFTVAGTNGK
CCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
GTTCCTLEAILLAAGLRVGVYSSPHLLRYTERVRIQGQELSEAEHSHSFAQIEAGRGDIS
CCCHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHCCCHHHHHHHHCCCCEEEECCCCCEE
LTYFEFGTLSALQLFKQAKLDVVILEVGLGGRLDATNIVDSDVAAITSIALDHTDWLGYD
EEEEECCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCHHHCCCC
RESIGREKAGVFRGGKPAVVGEPDMPQSIADVAAELGAQLYRRDVAWKFSQQEPFDQQEP
HHHHCCHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCC
VDQQINGWHWQCGERQLTGLPVPNVPLANAATALAVLHYSELPLSDEAIRQGLQAASLPG
HHHHCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCHHHCCCCC
RFQVVSEQPLLILDVAHNPHAARYLVNRLAQVINPVNASKQGKVRAVVGMLSDKDIAGTL
CEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCHHEEHHHHCCCCHHHHH
ACLSERVDEWYCAPLEGPRGASAGQLAEHLVSARQFSDVETAWRQAMQDADTQDVVIVCG
HHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
SFHTVAHVMAALHL
CHHHHHHHHHHHCC
>Mature Secondary Structure
MNNHQTPQATSPLAAWLCYLEHLHSQPIELGLERVKQVAERLDLLKPAPKIFTVAGTNGK
CCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
GTTCCTLEAILLAAGLRVGVYSSPHLLRYTERVRIQGQELSEAEHSHSFAQIEAGRGDIS
CCCHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHCCCHHHHHHHHCCCCEEEECCCCCEE
LTYFEFGTLSALQLFKQAKLDVVILEVGLGGRLDATNIVDSDVAAITSIALDHTDWLGYD
EEEEECCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCHHHCCCC
RESIGREKAGVFRGGKPAVVGEPDMPQSIADVAAELGAQLYRRDVAWKFSQQEPFDQQEP
HHHHCCHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCC
VDQQINGWHWQCGERQLTGLPVPNVPLANAATALAVLHYSELPLSDEAIRQGLQAASLPG
HHHHCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCHHHCCCCC
RFQVVSEQPLLILDVAHNPHAARYLVNRLAQVINPVNASKQGKVRAVVGMLSDKDIAGTL
CEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCHHEEHHHHCCCCHHHHH
ACLSERVDEWYCAPLEGPRGASAGQLAEHLVSARQFSDVETAWRQAMQDADTQDVVIVCG
HHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
SFHTVAHVMAALHL
CHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3040739; 3040734; 9205837; 9278503; 1989505 [H]