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The map label for this gene is vacJ [H]

Identifier: 218929815

GI number: 218929815

Start: 3073614

End: 3074378

Strand: Direct

Name: vacJ [H]

Synonym: YPO2743

Alternate gene names: 218929815

Gene position: 3073614-3074378 (Clockwise)

Preceding gene: 218929814

Following gene: 218929817

Centisome position: 66.05

GC content: 51.5

Gene sequence:

>765_bases
ATGAACTTCCGCCTTACTGGGTTGGCTTTCGCAAGCGTACTGTTAGTTGGGTGCGCCAGTAGTTCACCGGATCACATGGA
GCAGGGCCGCTCTGATCCGCTGGAAGGTTTCAACCGGGCAATGTTCAATTTTAACTATGAGGTCCTCGACCCTTATGTGG
TGCGTCCGGTCGCCGTCGTCTGGCGTGATTATGTGCCGCAACCAGCACGTAACGGTATGAGCAACTTTTTGGGTAACCTC
GAAGAACCCGCCAGTATGGTGAACAGCTTCCTGGTCGGTGATCCATATAATGCGATGAAGCATTTTAACCGCTTCTTCCT
GAATACCGTTTTGGGTATGGGCGGTTTGATCGATGTCGCCGGGATGGCTAATCCGAAACTGGCAAAACAAGTGCCTCAAC
GTTTTGGTAGTACATTAGGCCACTATGATGTGGGCTACGGCCCGTATGTGGTGCTACCAGGGTATGGCAGTTTTACCCTC
CGCGAAGATGGCGGCCAGTTGGCAGATACGGTGTATCCGGTACTGAGTTATCTGACGTTCTGGATGGCCGCAGGGAAATG
GGTGCTTGAAGGGGTTGAAACCCGCGCACAGCTATTGGACTCTGATGGTTTAGTGCGTAATTCTTCAGATCCTTATCTCA
TGATACGCGAGGCTTATTTCCAGCGTCATGATTTCCTGGCGAATGGTGGCCAACTGAAACCTGAAGTTAACTCGAATGCT
CAGGCTATTCAGGGCGATCTGGATAGCATAGATGCCGCCGATTGA

Upstream 100 bases:

>100_bases
TCAAAGGCAATGGGCCGTCGAGTGAGCAAAAAATAGGCGTTCAAATAAATAAACAAATCCCTTAAGTGAAAGGGATAGAC
AGACTTTCATGGAGAAAAAT

Downstream 100 bases:

>100_bases
GACTCATTGATATTAACTGGTCTAGCGCAGTACTCACCGCCGTATTCAAAATAGAAAAGCGCCATCAGGCGCTTTTCTAT
TGGATAAGGTGATGAATGAC

Product: VacJ lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MNFRLTGLAFASVLLVGCASSSPDHMEQGRSDPLEGFNRAMFNFNYEVLDPYVVRPVAVVWRDYVPQPARNGMSNFLGNL
EEPASMVNSFLVGDPYNAMKHFNRFFLNTVLGMGGLIDVAGMANPKLAKQVPQRFGSTLGHYDVGYGPYVVLPGYGSFTL
REDGGQLADTVYPVLSYLTFWMAAGKWVLEGVETRAQLLDSDGLVRNSSDPYLMIREAYFQRHDFLANGGQLKPEVNSNA
QAIQGDLDSIDAAD

Sequences:

>Translated_254_residues
MNFRLTGLAFASVLLVGCASSSPDHMEQGRSDPLEGFNRAMFNFNYEVLDPYVVRPVAVVWRDYVPQPARNGMSNFLGNL
EEPASMVNSFLVGDPYNAMKHFNRFFLNTVLGMGGLIDVAGMANPKLAKQVPQRFGSTLGHYDVGYGPYVVLPGYGSFTL
REDGGQLADTVYPVLSYLTFWMAAGKWVLEGVETRAQLLDSDGLVRNSSDPYLMIREAYFQRHDFLANGGQLKPEVNSNA
QAIQGDLDSIDAAD
>Mature_254_residues
MNFRLTGLAFASVLLVGCASSSPDHMEQGRSDPLEGFNRAMFNFNYEVLDPYVVRPVAVVWRDYVPQPARNGMSNFLGNL
EEPASMVNSFLVGDPYNAMKHFNRFFLNTVLGMGGLIDVAGMANPKLAKQVPQRFGSTLGHYDVGYGPYVVLPGYGSFTL
REDGGQLADTVYPVLSYLTFWMAAGKWVLEGVETRAQLLDSDGLVRNSSDPYLMIREAYFQRHDFLANGGQLKPEVNSNA
QAIQGDLDSIDAAD

Specific function: Actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane [H]

COG id: COG2853

COG function: function code M; Surface lipoprotein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaA family [H]

Homologues:

Organism=Escherichia coli, GI1788688, Length=252, Percent_Identity=75, Blast_Score=397, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007428 [H]

Pfam domain/function: PF04333 VacJ [H]

EC number: NA

Molecular weight: Translated: 27997; Mature: 27997

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFRLTGLAFASVLLVGCASSSPDHMEQGRSDPLEGFNRAMFNFNYEVLDPYVVRPVAVV
CCEEEHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHCCCHHHCCHHHHHHHHHH
WRDYVPQPARNGMSNFLGNLEEPASMVNSFLVGDPYNAMKHFNRFFLNTVLGMGGLIDVA
HHHHCCCHHHCCHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHC
GMANPKLAKQVPQRFGSTLGHYDVGYGPYVVLPGYGSFTLREDGGQLADTVYPVLSYLTF
CCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHHH
WMAAGKWVLEGVETRAQLLDSDGLVRNSSDPYLMIREAYFQRHDFLANGGQLKPEVNSNA
HHHHHHHHHHHHHHHHHHHCCCCCEECCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCH
QAIQGDLDSIDAAD
HHHHCCCCCCCCCC
>Mature Secondary Structure
MNFRLTGLAFASVLLVGCASSSPDHMEQGRSDPLEGFNRAMFNFNYEVLDPYVVRPVAVV
CCEEEHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHCCCHHHCCHHHHHHHHHH
WRDYVPQPARNGMSNFLGNLEEPASMVNSFLVGDPYNAMKHFNRFFLNTVLGMGGLIDVA
HHHHCCCHHHCCHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHC
GMANPKLAKQVPQRFGSTLGHYDVGYGPYVVLPGYGSFTLREDGGQLADTVYPVLSYLTF
CCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHHH
WMAAGKWVLEGVETRAQLLDSDGLVRNSSDPYLMIREAYFQRHDFLANGGQLKPEVNSNA
HHHHHHHHHHHHHHHHHHHCCCCCEECCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCH
QAIQGDLDSIDAAD
HHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]