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The map label for this gene is seqA [H]

Identifier: 218929758

GI number: 218929758

Start: 3009138

End: 3009665

Strand: Direct

Name: seqA [H]

Synonym: YPO2685

Alternate gene names: 218929758

Gene position: 3009138-3009665 (Clockwise)

Preceding gene: 218929755

Following gene: 218929759

Centisome position: 64.66

GC content: 50.76

Gene sequence:

>528_bases
ATGAAAACTATTGAACTCGACGAAGAACTTTATCGCTATATCGCCAGCCATACGCAACACATAGGTGAGAGCGCGTCAGA
TATCTTACGGCGTATGCTGAAGTTTACTGCCGGTCAGCCGGTAACTGACCTGTCTGTCAGCGGTGCTGCTGTGAAAGTGA
CTGCCCAGCCAGCCCCACGGGATCGGGTTCGTGCCGTCCGTGAGTTGCTGCTGTCCGATGAATATGCAGAACAGAGTAGG
GCGGTTAACCGCTTTATGTTGGTATTATCGACCTTATATACCTTAGATGCGCAGGCGTTTACTGAGGCAACTGAATCTTT
GCATGGTCGTACCCGAGTCTACTTTGCCGGTGATCAACAGACATTGCTGCAACACGGGACGCATACTAAACCCAAGCATG
TGCCAGGTACGCCATTCTGGGTTATCACCAATACCAATACTGAGCGTAAGCGCAGTATGGTACAGCACATCATGCTGGCA
ATGCAGTTCCCGCCGGAGTTGACTGACAAAGTGTGCGGAACTATCTGA

Upstream 100 bases:

>100_bases
TAAAACCAGATGGTTGAAAAAATGTAGGTCAAAATTAAACTTTTCTTTGATTGCTATAAAAACACTATAAAAATCAACTG
CATTATTGTTTTGGATAAAG

Downstream 100 bases:

>100_bases
TTTCATTGAAACCATCCTCTTTCTCAGCCAATGGCAACGCCCTTGCTGAGGACGTGTATAGGAGTAGTGACTGTGGCGAA
TCACCCTCGGGCTGGGCAAC

Product: replication initiation regulator SeqA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MKTIELDEELYRYIASHTQHIGESASDILRRMLKFTAGQPVTDLSVSGAAVKVTAQPAPRDRVRAVRELLLSDEYAEQSR
AVNRFMLVLSTLYTLDAQAFTEATESLHGRTRVYFAGDQQTLLQHGTHTKPKHVPGTPFWVITNTNTERKRSMVQHIMLA
MQFPPELTDKVCGTI

Sequences:

>Translated_175_residues
MKTIELDEELYRYIASHTQHIGESASDILRRMLKFTAGQPVTDLSVSGAAVKVTAQPAPRDRVRAVRELLLSDEYAEQSR
AVNRFMLVLSTLYTLDAQAFTEATESLHGRTRVYFAGDQQTLLQHGTHTKPKHVPGTPFWVITNTNTERKRSMVQHIMLA
MQFPPELTDKVCGTI
>Mature_175_residues
MKTIELDEELYRYIASHTQHIGESASDILRRMLKFTAGQPVTDLSVSGAAVKVTAQPAPRDRVRAVRELLLSDEYAEQSR
AVNRFMLVLSTLYTLDAQAFTEATESLHGRTRVYFAGDQQTLLQHGTHTKPKHVPGTPFWVITNTNTERKRSMVQHIMLA
MQFPPELTDKVCGTI

Specific function: Negative regulator of replication initiation. Required for sequestration, a process that blocks secondary initiation events. Could block the allosteric transition of dnaA from an inactive form to an active form. Binds hemimethylated oriC. This process has

COG id: COG3057

COG function: function code L; Negative regulator of replication initiationR

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786903, Length=182, Percent_Identity=73.0769230769231, Blast_Score=271, Evalue=1e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005621
- InterPro:   IPR010985 [H]

Pfam domain/function: PF03925 SeqA [H]

EC number: NA

Molecular weight: Translated: 19742; Mature: 19742

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIELDEELYRYIASHTQHIGESASDILRRMLKFTAGQPVTDLSVSGAAVKVTAQPAPR
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCH
DRVRAVRELLLSDEYAEQSRAVNRFMLVLSTLYTLDAQAFTEATESLHGRTRVYFAGDQQ
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHH
TLLQHGTHTKPKHVPGTPFWVITNTNTERKRSMVQHIMLAMQFPPELTDKVCGTI
HHHHCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
>Mature Secondary Structure
MKTIELDEELYRYIASHTQHIGESASDILRRMLKFTAGQPVTDLSVSGAAVKVTAQPAPR
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCH
DRVRAVRELLLSDEYAEQSRAVNRFMLVLSTLYTLDAQAFTEATESLHGRTRVYFAGDQQ
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHH
TLLQHGTHTKPKHVPGTPFWVITNTNTERKRSMVQHIMLAMQFPPELTDKVCGTI
HHHHCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]