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The map label for this gene is ubiF [H]

Identifier: 218929702

GI number: 218929702

Start: 2944454

End: 2945635

Strand: Direct

Name: ubiF [H]

Synonym: YPO2621

Alternate gene names: 218929702

Gene position: 2944454-2945635 (Clockwise)

Preceding gene: 218929692

Following gene: 218929703

Centisome position: 63.27

GC content: 53.47

Gene sequence:

>1182_bases
ATGAACAAATCGCAACCAAATTATGATGTTGTGGTCGTTGGCGGTGGCATGGTAGGTGCTGCGGCGGCGTTGGGGCTGGC
ACAAATTGGCTGGTCGGTGGCGTTACTGGAACATGATGCTCCTGCACCGTTTGACAAGGATAGCGTACCGGATTTGCGGG
TTTCTGCTCTTGGTTGTACTTCGGTGGCGTTATTGAAGCAGCTTGGTGCTTGGCCTCAAGTACAACAGATGCGTTATGCA
CCTTATCGTCGCTTGGAAACCTGGGAACAACCGGGGTCACAGGTGGTGTTTGATGCGGCCTCGCTGTCTTTACCTGAGCT
GGGCTTTATGGTCGAGAACCGGATTTTGCAGCTTGCTCTATGGCAACAGTTTGCTGAATGCCCTAATTTGACGCTGTTGT
GCCCGTCACGGCTACAAAGCATGGTTAGAATAGATGACTACTGGAAAGTCACATTGAATGAACAGGAGGAGATACAGGGC
CGTTTAGTGATAGGTGCCGATGGTGCGAACTCGTTGGTGAGGCGTTTGGCGGGGATAGGCACCAGCGGTTGGCAGTATCG
CCAGTCTTGCATGTTGATCACCGTAGAAACTGATGTCATGCAGCAAGATACGACATGGCAGCAATTCTTCCCGACCGGGC
CGCGTGCTTTCCTACCCCTGTTTGATCACTGGGCATCATTGGTTTGGTACGACAGCCCTCAACGTATTCGTCAGCTTCAG
GCGATGTCGATGGCACAACTGAGCCAGGAAATCGCAGCCTTCTTCCCCTCTCGTTTGGGGGCTGTCAAGGCGATTGCCGC
TGGCGCTTTCCCGCTGGTTCGTCGCCATGCGCAGCAGTACGTAAAACCGGGGTTGGTATTATTGGGAGATGCCGCTCATA
CCATCAATCCATTGGCTGGGCAGGGGGTCAACTTGGGATACCGTGATGTTGACGCATTGCTGGAGGTGCTTAGTCAGGCC
CGTGAGTTGGCTGAACCTTGGCACAGTGAGCAGGTTTTACTGCGTTACCAGCGTCGCCGCCGCACCGATAATCTCATGAT
GCAAAGTGGCATGGATCTGTTTTACACGGCCTTTAGTAATGATCTGCCGGCGGTTAAGTTTGCACGTAATCTGGCGTTGA
TGGTGGCCCAGCGTGCCGGGAAGCTGAAAGAACATGCATTGAGGTATGCGTTGGGGTTGTAG

Upstream 100 bases:

>100_bases
CGTTGAGTTATTCTGTTGAGTGATAAAAAATCCGTTACACTGCGGTTAGACAAAACCTTGCTAATGCAAGAAGTACATTC
ACTTTTAAAGCATAGAAAAA

Downstream 100 bases:

>100_bases
GGAGATTTTGTTTTTTTAGGGAAAATCGGCGTAGGCTTTGGTTGATGGGATTCGGTTGATTTGGTGATTGTTCGGTTGTG
ATGGCTTCGCGGCTTATCTA

Product: 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MNKSQPNYDVVVVGGGMVGAAAALGLAQIGWSVALLEHDAPAPFDKDSVPDLRVSALGCTSVALLKQLGAWPQVQQMRYA
PYRRLETWEQPGSQVVFDAASLSLPELGFMVENRILQLALWQQFAECPNLTLLCPSRLQSMVRIDDYWKVTLNEQEEIQG
RLVIGADGANSLVRRLAGIGTSGWQYRQSCMLITVETDVMQQDTTWQQFFPTGPRAFLPLFDHWASLVWYDSPQRIRQLQ
AMSMAQLSQEIAAFFPSRLGAVKAIAAGAFPLVRRHAQQYVKPGLVLLGDAAHTINPLAGQGVNLGYRDVDALLEVLSQA
RELAEPWHSEQVLLRYQRRRRTDNLMMQSGMDLFYTAFSNDLPAVKFARNLALMVAQRAGKLKEHALRYALGL

Sequences:

>Translated_393_residues
MNKSQPNYDVVVVGGGMVGAAAALGLAQIGWSVALLEHDAPAPFDKDSVPDLRVSALGCTSVALLKQLGAWPQVQQMRYA
PYRRLETWEQPGSQVVFDAASLSLPELGFMVENRILQLALWQQFAECPNLTLLCPSRLQSMVRIDDYWKVTLNEQEEIQG
RLVIGADGANSLVRRLAGIGTSGWQYRQSCMLITVETDVMQQDTTWQQFFPTGPRAFLPLFDHWASLVWYDSPQRIRQLQ
AMSMAQLSQEIAAFFPSRLGAVKAIAAGAFPLVRRHAQQYVKPGLVLLGDAAHTINPLAGQGVNLGYRDVDALLEVLSQA
RELAEPWHSEQVLLRYQRRRRTDNLMMQSGMDLFYTAFSNDLPAVKFARNLALMVAQRAGKLKEHALRYALGL
>Mature_393_residues
MNKSQPNYDVVVVGGGMVGAAAALGLAQIGWSVALLEHDAPAPFDKDSVPDLRVSALGCTSVALLKQLGAWPQVQQMRYA
PYRRLETWEQPGSQVVFDAASLSLPELGFMVENRILQLALWQQFAECPNLTLLCPSRLQSMVRIDDYWKVTLNEQEEIQG
RLVIGADGANSLVRRLAGIGTSGWQYRQSCMLITVETDVMQQDTTWQQFFPTGPRAFLPLFDHWASLVWYDSPQRIRQLQ
AMSMAQLSQEIAAFFPSRLGAVKAIAAGAFPLVRRHAQQYVKPGLVLLGDAAHTINPLAGQGVNLGYRDVDALLEVLSQA
RELAEPWHSEQVLLRYQRRRRTDNLMMQSGMDLFYTAFSNDLPAVKFARNLALMVAQRAGKLKEHALRYALGL

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=386, Percent_Identity=30.0518134715026, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI32967307, Length=343, Percent_Identity=29.4460641399417, Blast_Score=143, Evalue=3e-34,
Organism=Escherichia coli, GI1786883, Length=390, Percent_Identity=63.8461538461538, Blast_Score=496, Evalue=1e-142,
Organism=Escherichia coli, GI1789273, Length=388, Percent_Identity=37.3711340206186, Blast_Score=243, Evalue=1e-65,
Organism=Escherichia coli, GI1789274, Length=376, Percent_Identity=31.6489361702128, Blast_Score=171, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI25146631, Length=444, Percent_Identity=28.3783783783784, Blast_Score=169, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6321694, Length=285, Percent_Identity=28.4210526315789, Blast_Score=105, Evalue=9e-24,
Organism=Drosophila melanogaster, GI19920760, Length=433, Percent_Identity=28.4064665127021, Blast_Score=171, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 43798; Mature: 43798

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKSQPNYDVVVVGGGMVGAAAALGLAQIGWSVALLEHDAPAPFDKDSVPDLRVSALGCT
CCCCCCCEEEEEECCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCEEHHHHHH
SVALLKQLGAWPQVQQMRYAPYRRLETWEQPGSQVVFDAASLSLPELGFMVENRILQLAL
HHHHHHHHCCCHHHHHHHCCHHHHHHHHHCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHH
WQQFAECPNLTLLCPSRLQSMVRIDDYWKVTLNEQEEIQGRLVIGADGANSLVRRLAGIG
HHHHHHCCCCEEECHHHHHHHHHHCCEEEEEECCHHCCCCEEEEECCCHHHHHHHHHCCC
TSGWQYRQSCMLITVETDVMQQDTTWQQFFPTGPRAFLPLFDHWASLVWYDSPQRIRQLQ
CCCHHCCCCEEEEEEEHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHEEECCHHHHHHHH
AMSMAQLSQEIAAFFPSRLGAVKAIAAGAFPLVRRHAQQYVKPGLVLLGDAAHTINPLAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHCCCCCC
QGVNLGYRDVDALLEVLSQARELAEPWHSEQVLLRYQRRRRTDNLMMQSGMDLFYTAFSN
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHEEHHHCC
DLPAVKFARNLALMVAQRAGKLKEHALRYALGL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKSQPNYDVVVVGGGMVGAAAALGLAQIGWSVALLEHDAPAPFDKDSVPDLRVSALGCT
CCCCCCCEEEEEECCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCEEHHHHHH
SVALLKQLGAWPQVQQMRYAPYRRLETWEQPGSQVVFDAASLSLPELGFMVENRILQLAL
HHHHHHHHCCCHHHHHHHCCHHHHHHHHHCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHH
WQQFAECPNLTLLCPSRLQSMVRIDDYWKVTLNEQEEIQGRLVIGADGANSLVRRLAGIG
HHHHHHCCCCEEECHHHHHHHHHHCCEEEEEECCHHCCCCEEEEECCCHHHHHHHHHCCC
TSGWQYRQSCMLITVETDVMQQDTTWQQFFPTGPRAFLPLFDHWASLVWYDSPQRIRQLQ
CCCHHCCCCEEEEEEEHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHEEECCHHHHHHHH
AMSMAQLSQEIAAFFPSRLGAVKAIAAGAFPLVRRHAQQYVKPGLVLLGDAAHTINPLAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHCCCCCC
QGVNLGYRDVDALLEVLSQARELAEPWHSEQVLLRYQRRRRTDNLMMQSGMDLFYTAFSN
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHEEHHHCC
DLPAVKFARNLALMVAQRAGKLKEHALRYALGL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]