Click here to switch to the map view.

The map label for this gene is dacA [H]

Identifier: 218929682

GI number: 218929682

Start: 2921606

End: 2922808

Strand: Reverse

Name: dacA [H]

Synonym: YPO2601

Alternate gene names: 218929682

Gene position: 2922808-2921606 (Counterclockwise)

Preceding gene: 218929683

Following gene: 218929681

Centisome position: 62.81

GC content: 48.71

Gene sequence:

>1203_bases
ATGAAATATGTAATTACCTCTCGCATTATCAAGAGCCTAGCGCTCGGCACTGTTATTGTCATGAGCGCTGCTAACGCTGA
TGACGTCAATCTGAAAACCATGATCCCCGGCGTACCGCAGATCGACGCAGAAGCCTACATCCTAATTGATTACAACTCCG
GGAAAGTATTGGCGGAAATGAACGCGGATACGCGCCGCAACCCAGCCAGTTTGACCAAAATGATGACCAGCTACGTCATC
GGCCAGGCGATTAAATCAGGGAAAATCGGGGCTGAAGACATGGTCACCGTGGGTAAAGATGCCTGGGCTACCGGTAACCC
GGAATTTAAAGGCTCCTCCCTGATGTTTCTGAAACCGGGCGACCGTGTGCCGGTGTCTAAACTGACCCGTGGTATCAACT
TACAATCGGGTAACGATGCCTGCGTCGCGATGGCCGATTATGTCGCGGGGAGTCAGGACTCATTCGTTAACCTGATGAAC
AACTATGTTAAGGCGCTGGGCTTACAAAATACCCACTTCAAAACTGTTCACGGTTTAGATGCGGAAGGCCAGTTCAGCTC
TGCACGCGATATGGCATTGATTGGTCAAGCCCTGATCCGTGATGTGCCAGATGAGTATGCTATCTATAAAGAGAAAGAGT
TTACCTTCAACAATATCCGCCAGACAAACCGTAACGGTTTACTGTGGGACACCAGTTTGAACGTAGATGGCATCAAAACC
GGTCACACCGAGGCAGCAGGTTATAATCTGGTCGCATCAGCGACTGATGGACAGATGCGTTTGATTTCAGTCGTTTTAGG
CGGCCATACCTTTAAGGGCCGTGAAACTGAAAGTAAAAAACTGCTGACCTGGGGGTTCCGTTTCTTTGAAACCGTCGCAC
CGCTGAAAGTCGGTAAAGAGTTTGCCTCAGAACCGGTCTGGTTTGGCGATAGTGATCGTGTGCAGTTAGGTGTAGATAAA
GATGCTTATCTGACTATTCCACGTGGCCGAATGAAAGACCTGAAAGCCAGCTATGTGCTGAATACACCTGAAATTCATGC
GCCACTGGCGAAAAACCAAGTTGTGGGAACCATTAACTTCCAGTTAGATGGTAAAACCATCGATCAGCGCCCATTGGTGG
TGATGAACGAAGTAAAAGAAGGTGGTTTCTTCAGCCGTATGGTGGATTATATCAAGCTGATGTTCCACCGCTGGTTTGGC
TAA

Upstream 100 bases:

>100_bases
ACGCCACAGGGCAAGGAGATTGTCGGATGTCTGCCGGGATCTCATCTGCTATAGTGTGGCATGTTCTTAACTTACTCCCA
CGGATGTTGTTGTCCTGATC

Downstream 100 bases:

>100_bases
TCCGCTAGCTTGAAAAACAGAAGAGACTTGAAAGCCAGTAGATTGGCCCCATATTGTGATTAATCTTATATTGCAAATAA
GCTGATCTTATCTGGCGAAC

Product: D-alanyl-D-alanine carboxypeptidase fraction A

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MKYVITSRIIKSLALGTVIVMSAANADDVNLKTMIPGVPQIDAEAYILIDYNSGKVLAEMNADTRRNPASLTKMMTSYVI
GQAIKSGKIGAEDMVTVGKDAWATGNPEFKGSSLMFLKPGDRVPVSKLTRGINLQSGNDACVAMADYVAGSQDSFVNLMN
NYVKALGLQNTHFKTVHGLDAEGQFSSARDMALIGQALIRDVPDEYAIYKEKEFTFNNIRQTNRNGLLWDTSLNVDGIKT
GHTEAAGYNLVASATDGQMRLISVVLGGHTFKGRETESKKLLTWGFRFFETVAPLKVGKEFASEPVWFGDSDRVQLGVDK
DAYLTIPRGRMKDLKASYVLNTPEIHAPLAKNQVVGTINFQLDGKTIDQRPLVVMNEVKEGGFFSRMVDYIKLMFHRWFG

Sequences:

>Translated_400_residues
MKYVITSRIIKSLALGTVIVMSAANADDVNLKTMIPGVPQIDAEAYILIDYNSGKVLAEMNADTRRNPASLTKMMTSYVI
GQAIKSGKIGAEDMVTVGKDAWATGNPEFKGSSLMFLKPGDRVPVSKLTRGINLQSGNDACVAMADYVAGSQDSFVNLMN
NYVKALGLQNTHFKTVHGLDAEGQFSSARDMALIGQALIRDVPDEYAIYKEKEFTFNNIRQTNRNGLLWDTSLNVDGIKT
GHTEAAGYNLVASATDGQMRLISVVLGGHTFKGRETESKKLLTWGFRFFETVAPLKVGKEFASEPVWFGDSDRVQLGVDK
DAYLTIPRGRMKDLKASYVLNTPEIHAPLAKNQVVGTINFQLDGKTIDQRPLVVMNEVKEGGFFSRMVDYIKLMFHRWFG
>Mature_400_residues
MKYVITSRIIKSLALGTVIVMSAANADDVNLKTMIPGVPQIDAEAYILIDYNSGKVLAEMNADTRRNPASLTKMMTSYVI
GQAIKSGKIGAEDMVTVGKDAWATGNPEFKGSSLMFLKPGDRVPVSKLTRGINLQSGNDACVAMADYVAGSQDSFVNLMN
NYVKALGLQNTHFKTVHGLDAEGQFSSARDMALIGQALIRDVPDEYAIYKEKEFTFNNIRQTNRNGLLWDTSLNVDGIKT
GHTEAAGYNLVASATDGQMRLISVVLGGHTFKGRETESKKLLTWGFRFFETVAPLKVGKEFASEPVWFGDSDRVQLGVDK
DAYLTIPRGRMKDLKASYVLNTPEIHAPLAKNQVVGTINFQLDGKTIDQRPLVVMNEVKEGGFFSRMVDYIKLMFHRWFG

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=403, Percent_Identity=80.6451612903226, Blast_Score=689, Evalue=0.0,
Organism=Escherichia coli, GI1787062, Length=394, Percent_Identity=63.1979695431472, Blast_Score=519, Evalue=1e-148,
Organism=Escherichia coli, GI87082022, Length=384, Percent_Identity=48.9583333333333, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI87082054, Length=266, Percent_Identity=27.0676691729323, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 44089; Mature: 44089

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYVITSRIIKSLALGTVIVMSAANADDVNLKTMIPGVPQIDAEAYILIDYNSGKVLAEM
CCEEHHHHHHHHHHHHHEEEEECCCCCCCEEEEECCCCCCCCCCEEEEEEECCCEEEEEC
NADTRRNPASLTKMMTSYVIGQAIKSGKIGAEDMVTVGKDAWATGNPEFKGSSLMFLKPG
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHEEECCCCCCCCCCCCCCCEEEEECCC
DRVPVSKLTRGINLQSGNDACVAMADYVAGSQDSFVNLMNNYVKALGLQNTHFKTVHGLD
CCCCHHHHHCCCCCCCCCCCEEEEEHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCC
AEGQFSSARDMALIGQALIRDVPDEYAIYKEKEFTFNNIRQTNRNGLLWDTSLNVDGIKT
CCCCCCCCHHHHHHHHHHHHHCCCHHEEEECCCCCHHHHHHCCCCCEEEECCCCCCCCCC
GHTEAAGYNLVASATDGQMRLISVVLGGHTFKGRETESKKLLTWGFRFFETVAPLKVGKE
CCCCCCCCEEEEECCCCCEEEEEEEECCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
FASEPVWFGDSDRVQLGVDKDAYLTIPRGRMKDLKASYVLNTPEIHAPLAKNQVVGTINF
HHCCCCEECCCCCEEEECCCCCEEEECCCHHHHCCCEEEECCCCCCCCCCCCCEEEEEEE
QLDGKTIDQRPLVVMNEVKEGGFFSRMVDYIKLMFHRWFG
EECCCCCCCCCEEEEEHHHCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKYVITSRIIKSLALGTVIVMSAANADDVNLKTMIPGVPQIDAEAYILIDYNSGKVLAEM
CCEEHHHHHHHHHHHHHEEEEECCCCCCCEEEEECCCCCCCCCCEEEEEEECCCEEEEEC
NADTRRNPASLTKMMTSYVIGQAIKSGKIGAEDMVTVGKDAWATGNPEFKGSSLMFLKPG
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHEEECCCCCCCCCCCCCCCEEEEECCC
DRVPVSKLTRGINLQSGNDACVAMADYVAGSQDSFVNLMNNYVKALGLQNTHFKTVHGLD
CCCCHHHHHCCCCCCCCCCCEEEEEHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCC
AEGQFSSARDMALIGQALIRDVPDEYAIYKEKEFTFNNIRQTNRNGLLWDTSLNVDGIKT
CCCCCCCCHHHHHHHHHHHHHCCCHHEEEECCCCCHHHHHHCCCCCEEEECCCCCCCCCC
GHTEAAGYNLVASATDGQMRLISVVLGGHTFKGRETESKKLLTWGFRFFETVAPLKVGKE
CCCCCCCCEEEEECCCCCEEEEEEEECCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
FASEPVWFGDSDRVQLGVDKDAYLTIPRGRMKDLKASYVLNTPEIHAPLAKNQVVGTINF
HHCCCCEECCCCCEEEECCCCCEEEECCCHHHHCCCEEEECCCCCCCCCCCCCEEEEEEE
QLDGKTIDQRPLVVMNEVKEGGFFSRMVDYIKLMFHRWFG
EECCCCCCCCCEEEEEHHHCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]