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The map label for this gene is metG

Identifier: 218928663

GI number: 218928663

Start: 1729180

End: 1731207

Strand: Reverse

Name: metG

Synonym: YPO1522

Alternate gene names: 218928663

Gene position: 1731207-1729180 (Counterclockwise)

Preceding gene: 218928668

Following gene: 218928661

Centisome position: 37.2

GC content: 48.42

Gene sequence:

>2028_bases
ATGGCTCAAGTCGCGAAAAAAATATTGGTGACGTGCGCGCTACCATACGCTAACGGTTCTATTCATCTCGGCCACATGCT
CGAGCATATCCAGGCAGACATCTGGGTCCGTTTCCAGCGAATGCGCGGCAACCAGGTTCATTTTATCTGTGCAGATGATG
CCCACGGGACTCCTATCATGCTGAAAGCTCAGCAAATGGGTATTGAACCAGAGCAGATGATTGCCGAAATGAGCCAGGAG
CACCAACAGGATTTCGCCGGTTTTGCTATCAGTTATGATAACTATCACTCCACCCACAGTGATGAAAACCGTGAACTGTC
GAGCCTGATCTATGGCCGTCTGAAAGCCAACGGTTATATAAAGAACCGTACTATTTCTCAGCTCTATGATCCTGAGAAAG
GCATGTTTCTGCCCGATCGTTTCGTGAAAGGCACTTGCCCAAAATGCAAAGCCCCGGAACAGTACGGCGATAATTGTGAA
GTCTGTGGCGCAACCTACAGCCCAACTGAACTTATCGACCCCAAATCCGCCGTTTCTGGTGCGACACCAGTGATGCGTGA
ATCTGAACACTTCTTCTTCGATTTACCGGCTTTCAGTGACATGTTGCAAGCCTGGACACGCTCAGGTGCGCTGCAAGAAC
AAGTCGCAAACAAAATGCAGGAATGGTTTGATTCCGGCCTGCAACAGTGGGATATCACGCGTGATGCCCCTTATTTCGGT
TTCGAAGTACCGGATGCACCGGGTAAATATTTTTACGTCTGGTTAGATGCCCCAATCGGTTACATGGGGGCGTTTAAAAA
CCTATGTGATAAACGTGGTGACCTGGATTTTGATGAATTCTGGGGTAAAGACGCCAAAACTGACCTCTATCACTTCATCG
GTAAAGATATTGTTTACTTCCACAGTCTGTTCTGGCCAGCCATGTTGGAAGGCAGTAACTTCCGCAAGCCGACAAACCTG
TTTGTCCATGGCTATGTCACGGTAAACGGCGCGAAAATGTCTAAATCTCGCGGTACGTTTATTAAGGCGGGGACTTACCT
GAAATATCTGGATGCGGATTGCCTGCGTTACTACTACGCGGCGAAACTCTCTTCGCGTATTGATGATATCGATTTAAATC
TGGAAGATTTCGTCCAACGCGTTAACGCCGATATCGTTAATAAAGTGGTGAATCTGGCCTCACGTAATGCTGGTTTTATC
AATAAACGCTTTGCAGGCCAGTTAGCTGACCAATTGGCTGACCCGGTGTTGTATAAAACATTCACCGATGCCGCAACCAG
TATTGCTGATGCTTATAACAACCGTGAGTCAGGTAAAGCCATTCGTGAAATTATGGCGCTAGCTGATGTGGCTAATCGCT
ATGTTGATGAACAGGCTCCATGGGTGGTCGCTAAACAAGAAGGCCGCGATGCCGATCTACACGCTATCTGCTCGATGGGG
ATCAACCTATTCCGTGTCCTGATGACCTATCTGAAACCGGTATTACCCTCTCTGACAGAGCGTACCGAGGCCTTCCTCAA
TACCGAGCTAACTTGGGATAGCATTGAACAACCTCTGCTGGGCCACTCTATCACCGCTTTCAAGGCACTGTTTAATCGTA
TTGATTTAGACAAAGTAAACGAAATGGTCGCCTCGTCTAAAGAAGATATGGCACCTGCAACCAGGGTTACAGGCCCGTTA
GCCGATGATCCAATTCAGGAAACCATCAGTTTTGATGACTTTGCCAAAGTTGATATGCGAATTGCGTTGATTCAACAAGC
CGAATTTGTTGAAGGCTCAGATAAGTTATTAAAACTAACACTTGAACTTGGAGGGGAGACCCGCCAGGTATTCTCGGGTA
TCCGTTCAGCTTACCCTGACCCAAAAGCCTTGGAAGGCCGCATGACCGTGATGGTCGCTAACCTTGCCCCGAGAAAAATG
CGCTTTGGGGTTTCAGAAGGCATGGTGATGGCCGCAGGCCCCGGAGGTAGCGATATCTTCCTGCTTAGCCCTGACAGTGG
TGCGCAGCCAGGCATGCAAGTGAAGTGA

Upstream 100 bases:

>100_bases
ACTTAAGGTGCAGTACCCGCTCCCTTGCCCTAGCGGCAAACTCGCTTATCGGTTAACATCAAAGGCCCTTTAATCAACAT
AAAGAAAGCAATTTCCCACT

Downstream 100 bases:

>100_bases
TTTTCTTCTGGCGACTAAGCGAAGATACAATAGATAACTGAAAGATCCTTAAAGAAATAAATATCCTCCGGCATAGCCGG
AGGTTTTTCATATGCGCCTA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 675; Mature: 674

Protein sequence:

>675_residues
MAQVAKKILVTCALPYANGSIHLGHMLEHIQADIWVRFQRMRGNQVHFICADDAHGTPIMLKAQQMGIEPEQMIAEMSQE
HQQDFAGFAISYDNYHSTHSDENRELSSLIYGRLKANGYIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKAPEQYGDNCE
VCGATYSPTELIDPKSAVSGATPVMRESEHFFFDLPAFSDMLQAWTRSGALQEQVANKMQEWFDSGLQQWDITRDAPYFG
FEVPDAPGKYFYVWLDAPIGYMGAFKNLCDKRGDLDFDEFWGKDAKTDLYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNL
FVHGYVTVNGAKMSKSRGTFIKAGTYLKYLDADCLRYYYAAKLSSRIDDIDLNLEDFVQRVNADIVNKVVNLASRNAGFI
NKRFAGQLADQLADPVLYKTFTDAATSIADAYNNRESGKAIREIMALADVANRYVDEQAPWVVAKQEGRDADLHAICSMG
INLFRVLMTYLKPVLPSLTERTEAFLNTELTWDSIEQPLLGHSITAFKALFNRIDLDKVNEMVASSKEDMAPATRVTGPL
ADDPIQETISFDDFAKVDMRIALIQQAEFVEGSDKLLKLTLELGGETRQVFSGIRSAYPDPKALEGRMTVMVANLAPRKM
RFGVSEGMVMAAGPGGSDIFLLSPDSGAQPGMQVK

Sequences:

>Translated_675_residues
MAQVAKKILVTCALPYANGSIHLGHMLEHIQADIWVRFQRMRGNQVHFICADDAHGTPIMLKAQQMGIEPEQMIAEMSQE
HQQDFAGFAISYDNYHSTHSDENRELSSLIYGRLKANGYIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKAPEQYGDNCE
VCGATYSPTELIDPKSAVSGATPVMRESEHFFFDLPAFSDMLQAWTRSGALQEQVANKMQEWFDSGLQQWDITRDAPYFG
FEVPDAPGKYFYVWLDAPIGYMGAFKNLCDKRGDLDFDEFWGKDAKTDLYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNL
FVHGYVTVNGAKMSKSRGTFIKAGTYLKYLDADCLRYYYAAKLSSRIDDIDLNLEDFVQRVNADIVNKVVNLASRNAGFI
NKRFAGQLADQLADPVLYKTFTDAATSIADAYNNRESGKAIREIMALADVANRYVDEQAPWVVAKQEGRDADLHAICSMG
INLFRVLMTYLKPVLPSLTERTEAFLNTELTWDSIEQPLLGHSITAFKALFNRIDLDKVNEMVASSKEDMAPATRVTGPL
ADDPIQETISFDDFAKVDMRIALIQQAEFVEGSDKLLKLTLELGGETRQVFSGIRSAYPDPKALEGRMTVMVANLAPRKM
RFGVSEGMVMAAGPGGSDIFLLSPDSGAQPGMQVK
>Mature_674_residues
AQVAKKILVTCALPYANGSIHLGHMLEHIQADIWVRFQRMRGNQVHFICADDAHGTPIMLKAQQMGIEPEQMIAEMSQEH
QQDFAGFAISYDNYHSTHSDENRELSSLIYGRLKANGYIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKAPEQYGDNCEV
CGATYSPTELIDPKSAVSGATPVMRESEHFFFDLPAFSDMLQAWTRSGALQEQVANKMQEWFDSGLQQWDITRDAPYFGF
EVPDAPGKYFYVWLDAPIGYMGAFKNLCDKRGDLDFDEFWGKDAKTDLYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLF
VHGYVTVNGAKMSKSRGTFIKAGTYLKYLDADCLRYYYAAKLSSRIDDIDLNLEDFVQRVNADIVNKVVNLASRNAGFIN
KRFAGQLADQLADPVLYKTFTDAATSIADAYNNRESGKAIREIMALADVANRYVDEQAPWVVAKQEGRDADLHAICSMGI
NLFRVLMTYLKPVLPSLTERTEAFLNTELTWDSIEQPLLGHSITAFKALFNRIDLDKVNEMVASSKEDMAPATRVTGPLA
DDPIQETISFDDFAKVDMRIALIQQAEFVEGSDKLLKLTLELGGETRQVFSGIRSAYPDPKALEGRMTVMVANLAPRKMR
FGVSEGMVMAAGPGGSDIFLLSPDSGAQPGMQVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=566, Percent_Identity=27.5618374558304, Blast_Score=235, Evalue=9e-62,
Organism=Homo sapiens, GI24308436, Length=513, Percent_Identity=25.9259259259259, Blast_Score=167, Evalue=3e-41,
Organism=Escherichia coli, GI1788432, Length=677, Percent_Identity=84.7858197932053, Blast_Score=1228, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540794, Length=572, Percent_Identity=28.8461538461538, Blast_Score=230, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI212645096, Length=511, Percent_Identity=23.8747553816047, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71992781, Length=405, Percent_Identity=23.4567901234568, Blast_Score=109, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6321704, Length=559, Percent_Identity=28.8014311270125, Blast_Score=245, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6321610, Length=538, Percent_Identity=22.4907063197026, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24655311, Length=570, Percent_Identity=28.5964912280702, Blast_Score=232, Evalue=7e-61,
Organism=Drosophila melanogaster, GI21358483, Length=513, Percent_Identity=24.5614035087719, Blast_Score=135, Evalue=9e-32,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_YERP3 (A7FJJ6)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001401424.1
- ProteinModelPortal:   A7FJJ6
- STRING:   A7FJJ6
- GeneID:   5388024
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_2455
- eggNOG:   COG0143
- HOGENOM:   HBG721667
- OMA:   SAYQPEQ
- ProtClustDB:   PRK00133
- BioCyc:   YPSE349747:YPSIP31758_2455-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 75637; Mature: 75506

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 335-335

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQVAKKILVTCALPYANGSIHLGHMLEHIQADIWVRFQRMRGNQVHFICADDAHGTPIM
CHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEE
LKAQQMGIEPEQMIAEMSQEHQQDFAGFAISYDNYHSTHSDENRELSSLIYGRLKANGYI
EEHHHHCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEE
KNRTISQLYDPEKGMFLPDRFVKGTCPKCKAPEQYGDNCEVCGATYSPTELIDPKSAVSG
ECCCHHHHCCCCCCCCCCHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCHHHCCCHHHCCC
ATPVMRESEHFFFDLPAFSDMLQAWTRSGALQEQVANKMQEWFDSGLQQWDITRDAPYFG
CCCCCCCCCCEEEECHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCC
FEVPDAPGKYFYVWLDAPIGYMGAFKNLCDKRGDLDFDEFWGKDAKTDLYHFIGKDIVYF
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHH
HSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKMSKSRGTFIKAGTYLKYLDADCLRYYYA
HHHHHHHHHCCCCCCCCCEEEEEEEEEEECCEECCCCCCEEECCCHHHHHHHHHHHHHHH
AKLSSRIDDIDLNLEDFVQRVNADIVNKVVNLASRNAGFINKRFAGQLADQLADPVLYKT
HHHHCCCCCCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHH
FTDAATSIADAYNNRESGKAIREIMALADVANRYVDEQAPWVVAKQEGRDADLHAICSMG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHH
INLFRVLMTYLKPVLPSLTERTEAFLNTELTWDSIEQPLLGHSITAFKALFNRIDLDKVN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHCCHHHHHHHHHHHCCHHHHH
EMVASSKEDMAPATRVTGPLADDPIQETISFDDFAKVDMRIALIQQAEFVEGSDKLLKLT
HHHHCCHHHCCCHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEE
LELGGETRQVFSGIRSAYPDPKALEGRMTVMVANLAPRKMRFGVSEGMVMAAGPGGSDIF
ECCCCCHHHHHHHHHHCCCCCHHCCCEEEEEEECCCCHHHHCCCCCCEEEEECCCCCEEE
LLSPDSGAQPGMQVK
EECCCCCCCCCCCCC
>Mature Secondary Structure 
AQVAKKILVTCALPYANGSIHLGHMLEHIQADIWVRFQRMRGNQVHFICADDAHGTPIM
HHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEE
LKAQQMGIEPEQMIAEMSQEHQQDFAGFAISYDNYHSTHSDENRELSSLIYGRLKANGYI
EEHHHHCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEE
KNRTISQLYDPEKGMFLPDRFVKGTCPKCKAPEQYGDNCEVCGATYSPTELIDPKSAVSG
ECCCHHHHCCCCCCCCCCHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCHHHCCCHHHCCC
ATPVMRESEHFFFDLPAFSDMLQAWTRSGALQEQVANKMQEWFDSGLQQWDITRDAPYFG
CCCCCCCCCCEEEECHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCC
FEVPDAPGKYFYVWLDAPIGYMGAFKNLCDKRGDLDFDEFWGKDAKTDLYHFIGKDIVYF
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHH
HSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKMSKSRGTFIKAGTYLKYLDADCLRYYYA
HHHHHHHHHCCCCCCCCCEEEEEEEEEEECCEECCCCCCEEECCCHHHHHHHHHHHHHHH
AKLSSRIDDIDLNLEDFVQRVNADIVNKVVNLASRNAGFINKRFAGQLADQLADPVLYKT
HHHHCCCCCCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHH
FTDAATSIADAYNNRESGKAIREIMALADVANRYVDEQAPWVVAKQEGRDADLHAICSMG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHH
INLFRVLMTYLKPVLPSLTERTEAFLNTELTWDSIEQPLLGHSITAFKALFNRIDLDKVN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHCCHHHHHHHHHHHCCHHHHH
EMVASSKEDMAPATRVTGPLADDPIQETISFDDFAKVDMRIALIQQAEFVEGSDKLLKLT
HHHHCCHHHCCCHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEE
LELGGETRQVFSGIRSAYPDPKALEGRMTVMVANLAPRKMRFGVSEGMVMAAGPGGSDIF
ECCCCCHHHHHHHHHHCCCCCHHCCCEEEEEEECCCCHHHHCCCCCCEEEEECCCCCEEE
LLSPDSGAQPGMQVK
EECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA