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The map label for this gene is rlmI [H]

Identifier: 218928591

GI number: 218928591

Start: 1637479

End: 1638669

Strand: Reverse

Name: rlmI [H]

Synonym: YPO1445

Alternate gene names: 218928591

Gene position: 1638669-1637479 (Counterclockwise)

Preceding gene: 218928593

Following gene: 218928590

Centisome position: 35.21

GC content: 47.27

Gene sequence:

>1191_bases
ATGACCGTACGCCTTATTCTTGCTAAAGGACGTGAAAAATCCTTACTTCGTCGCCACCCATGGATTTTCTCTGGGGCGGT
TCAACGCCTTGAAGGTGATGCACTTTCCGGTGAAACCATAGATATCCTTGATAGTCAGGGAAAATGGTTGGCCCGTGCCG
CCTACTCGCCTGAATCACAAATTTTGGCACGAGTCTGGACGTTCCAACAAGATGAGGTTATAGATTGCGCATTCTTTATT
CGCCGCCTACAACAGGCTCAGAATTGGCGAGATTGGCTGGCACAGCGTGATGGCCTTAATGGCTACCGCCTGATTGCCGG
TGAATCGGATGGCCTGCCGGGCATTACCATTGACCGTTTCCAGAATTTCTTGGTGTTACAGTTACTTTCTGCCGGTGCCG
AATATCAACGTGAAACGCTGGTCAGTGCGTTACAACATTGCTATCCAGAATGTTCTATTTATGATCGTTCCGATGTTTCT
GTCCGTAAAAAAGAAGGTTTGCCACTGACTCAAGGCCTTATCTGTGGCGAAATGCCACCAGCCCTGCTGCCAATTAGCGA
AAATGGCATGCAACTCTTCGTTGATATCCAGCAAGGTCATAAAACGGGTTTCTATTTAGATCAACGCGATAGCCGCTTAG
CGGCCCGTAATTATGCCAATGGCCGTCGTGTTTTGAACTGCTTCTCATACACGGGGGCTTTTGCTGTCGCCGCGTTGATG
GGCAATTGCCAACAGGTCATTAGTGTTGATACATCGCAATCCGTGTTAGATATTGCGAAACAAAACATTGAACTGAACCA
GTTGGATCTGAGCAAAACGGAGTTTGTCCGTGACGATGTATTCCAATTGTTGCGCAGCTATCGCGCTCAAGGGGAAAAAT
TCGACCTGATCATTATGGATCCACCTAAATTTGTTGAGAATAAAAGCCAATTAGCCAGTGCATGCCGTGGCTATAAAGAT
ATCAATATGTTGGCGATTCAATTACTGCGCCCTGGCGGTATCTTGCTCAGTTTCTCTTGTTCAGGTTTGATGCCGGTCGA
TTTATTCCAGAAAATTTTGGCCGATGCCGCGTTAGATGCAGGCCATGACATACAGTTTATAGAGCAGTTCCGCCAAGCTG
CGGATCACCCAGTGATCGCGGCTTATCCAGAAGGTTTATATCTAAAAGGGTTCGCGTGTCGGGTAATGTAA

Upstream 100 bases:

>100_bases
TTGACATAATCATTCCCTCATCTGTGCGTGATAGTTTGCATTTTCTAGCACATACTCAGACAATATCGCTCTTTTTTACC
TAGATGGCAGCAACCTAATT

Downstream 100 bases:

>100_bases
CTTGAAATATCCTGCGACACCCTCATATACAAGACAGTGAAAAGTTCTCGGGAGGTTATCATGATCGCCAGCAAATTCGG
TATAGGCCAGCAGGTTCGTC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MTVRLILAKGREKSLLRRHPWIFSGAVQRLEGDALSGETIDILDSQGKWLARAAYSPESQILARVWTFQQDEVIDCAFFI
RRLQQAQNWRDWLAQRDGLNGYRLIAGESDGLPGITIDRFQNFLVLQLLSAGAEYQRETLVSALQHCYPECSIYDRSDVS
VRKKEGLPLTQGLICGEMPPALLPISENGMQLFVDIQQGHKTGFYLDQRDSRLAARNYANGRRVLNCFSYTGAFAVAALM
GNCQQVISVDTSQSVLDIAKQNIELNQLDLSKTEFVRDDVFQLLRSYRAQGEKFDLIIMDPPKFVENKSQLASACRGYKD
INMLAIQLLRPGGILLSFSCSGLMPVDLFQKILADAALDAGHDIQFIEQFRQAADHPVIAAYPEGLYLKGFACRVM

Sequences:

>Translated_396_residues
MTVRLILAKGREKSLLRRHPWIFSGAVQRLEGDALSGETIDILDSQGKWLARAAYSPESQILARVWTFQQDEVIDCAFFI
RRLQQAQNWRDWLAQRDGLNGYRLIAGESDGLPGITIDRFQNFLVLQLLSAGAEYQRETLVSALQHCYPECSIYDRSDVS
VRKKEGLPLTQGLICGEMPPALLPISENGMQLFVDIQQGHKTGFYLDQRDSRLAARNYANGRRVLNCFSYTGAFAVAALM
GNCQQVISVDTSQSVLDIAKQNIELNQLDLSKTEFVRDDVFQLLRSYRAQGEKFDLIIMDPPKFVENKSQLASACRGYKD
INMLAIQLLRPGGILLSFSCSGLMPVDLFQKILADAALDAGHDIQFIEQFRQAADHPVIAAYPEGLYLKGFACRVM
>Mature_395_residues
TVRLILAKGREKSLLRRHPWIFSGAVQRLEGDALSGETIDILDSQGKWLARAAYSPESQILARVWTFQQDEVIDCAFFIR
RLQQAQNWRDWLAQRDGLNGYRLIAGESDGLPGITIDRFQNFLVLQLLSAGAEYQRETLVSALQHCYPECSIYDRSDVSV
RKKEGLPLTQGLICGEMPPALLPISENGMQLFVDIQQGHKTGFYLDQRDSRLAARNYANGRRVLNCFSYTGAFAVAALMG
NCQQVISVDTSQSVLDIAKQNIELNQLDLSKTEFVRDDVFQLLRSYRAQGEKFDLIIMDPPKFVENKSQLASACRGYKDI
NMLAIQLLRPGGILLSFSCSGLMPVDLFQKILADAALDAGHDIQFIEQFRQAADHPVIAAYPEGLYLKGFACRVM

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=396, Percent_Identity=80.5555555555556, Blast_Score=674, Evalue=0.0,
Organism=Escherichia coli, GI1787181, Length=267, Percent_Identity=27.3408239700375, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 44482; Mature: 44350

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVRLILAKGREKSLLRRHPWIFSGAVQRLEGDALSGETIDILDSQGKWLARAAYSPESQ
CEEEEEEECCCHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEECCCCHHH
ILARVWTFQQDEVIDCAFFIRRLQQAQNWRDWLAQRDGLNGYRLIAGESDGLPGITIDRF
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHH
QNFLVLQLLSAGAEYQRETLVSALQHCYPECSIYDRSDVSVRKKEGLPLTQGLICGEMPP
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCHHHCCCCCCCCC
ALLPISENGMQLFVDIQQGHKTGFYLDQRDSRLAARNYANGRRVLNCFSYTGAFAVAALM
CEEEECCCCCEEEEEEECCCCCCCEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
GNCQQVISVDTSQSVLDIAKQNIELNQLDLSKTEFVRDDVFQLLRSYRAQGEKFDLIIMD
HCCHHHHCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
PPKFVENKSQLASACRGYKDINMLAIQLLRPGGILLSFSCSGLMPVDLFQKILADAALDA
CCHHHCCHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCC
GHDIQFIEQFRQAADHPVIAAYPEGLYLKGFACRVM
CCCHHHHHHHHHHCCCCEEEECCCCEEECCEEEECC
>Mature Secondary Structure 
TVRLILAKGREKSLLRRHPWIFSGAVQRLEGDALSGETIDILDSQGKWLARAAYSPESQ
EEEEEEECCCHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEECCCCHHH
ILARVWTFQQDEVIDCAFFIRRLQQAQNWRDWLAQRDGLNGYRLIAGESDGLPGITIDRF
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHH
QNFLVLQLLSAGAEYQRETLVSALQHCYPECSIYDRSDVSVRKKEGLPLTQGLICGEMPP
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCHHHCCCCCCCCC
ALLPISENGMQLFVDIQQGHKTGFYLDQRDSRLAARNYANGRRVLNCFSYTGAFAVAALM
CEEEECCCCCEEEEEEECCCCCCCEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
GNCQQVISVDTSQSVLDIAKQNIELNQLDLSKTEFVRDDVFQLLRSYRAQGEKFDLIIMD
HCCHHHHCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
PPKFVENKSQLASACRGYKDINMLAIQLLRPGGILLSFSCSGLMPVDLFQKILADAALDA
CCHHHCCHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCC
GHDIQFIEQFRQAADHPVIAAYPEGLYLKGFACRVM
CCCHHHHHHHHHHCCCCEEEECCCCEEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA