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The map label for this gene is ymfK [C]

Identifier: 218928387

GI number: 218928387

Start: 1392937

End: 1393659

Strand: Reverse

Name: ymfK [C]

Synonym: YPO1233

Alternate gene names: 218928387

Gene position: 1393659-1392937 (Counterclockwise)

Preceding gene: 218928410

Following gene: 218928386

Centisome position: 29.95

GC content: 47.72

Gene sequence:

>723_bases
ATGAAAAAGAAGCCATTAACGCCCGAACAGTTAGATGACGCTAAGCGGCTGAAAGAGCTGTTTAATGCGAAGAAAAAAGC
ACTGGGTATATCCCAGGAATCTGTTGCACATGAATTGGGCGTCGGCCAAAGTGCCGTCAACCAGTTCCTCAACGGCGTAA
ACCCGCTCAATATCACCAACGCGGCTGCTTTTGCAAAAGTACTGAATGAACCCATCAGTAGCTTTAGCCCTTCTCTGGCC
AAAGAGTTGGCAAAAATGGCGGAGAGCCTGTCCATCACTACCCGCTCAGGTGCAAAGGATAAACCCGCGGTTTCCGTCGC
TAATAGCTATCCGTTAATCAGTTGGATAAGCGCGGGTAACTGGTATGAAGCGATAGAGCCGTATACGCTGCGTGACATTG
AGATCTGGCCTGAATCGACCAAGCATGCCCATGAGAGTGCATTTTGGTTGAGTGTAAAAGGCGATTCCATGACCTCGCCA
TCAGGTATCAGCTTCCCAGAAGGGATGATTATCCTGGTTGACCCAGAAAAAGAGCCCATGCCGGGTAATTTTGTGATCGC
CAAACTGACGGATGATAACGAAGCAACATTTAAGAAGTTAGTGGTTGATGCGGGCATTAAGTACTTGAAACCCTTGAATC
CCGCGTATCGGTTAATTGAACTGAACGGTAACTGTAAGATACTCGGTGTGGTTGTTGATGCCCGTTGGTTGCAAATAGAT
TAG

Upstream 100 bases:

>100_bases
CACTTCCCTTATACATTTCGATTGATTATTACCGCAAGTGATTTTATAGTCAACACCGCAAGTGATTGGATATTATTGCT
AACGGTAATAAAATTGACTC

Downstream 100 bases:

>100_bases
CCAGGCCGTCCGTTTCCTTAAAAACCAATTACAAAATAAAATAACTAAATCATGTGTCTATACAACCAGTTATCTTTTTA
TATAAAAAATAACCGCCAGT

Product: putative prophage repressor protein

Products: NA

Alternate protein names: ORF-96 protein [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MKKKPLTPEQLDDAKRLKELFNAKKKALGISQESVAHELGVGQSAVNQFLNGVNPLNITNAAAFAKVLNEPISSFSPSLA
KELAKMAESLSITTRSGAKDKPAVSVANSYPLISWISAGNWYEAIEPYTLRDIEIWPESTKHAHESAFWLSVKGDSMTSP
SGISFPEGMIILVDPEKEPMPGNFVIAKLTDDNEATFKKLVVDAGIKYLKPLNPAYRLIELNGNCKILGVVVDARWLQID

Sequences:

>Translated_240_residues
MKKKPLTPEQLDDAKRLKELFNAKKKALGISQESVAHELGVGQSAVNQFLNGVNPLNITNAAAFAKVLNEPISSFSPSLA
KELAKMAESLSITTRSGAKDKPAVSVANSYPLISWISAGNWYEAIEPYTLRDIEIWPESTKHAHESAFWLSVKGDSMTSP
SGISFPEGMIILVDPEKEPMPGNFVIAKLTDDNEATFKKLVVDAGIKYLKPLNPAYRLIELNGNCKILGVVVDARWLQID
>Mature_240_residues
MKKKPLTPEQLDDAKRLKELFNAKKKALGISQESVAHELGVGQSAVNQFLNGVNPLNITNAAAFAKVLNEPISSFSPSLA
KELAKMAESLSITTRSGAKDKPAVSVANSYPLISWISAGNWYEAIEPYTLRDIEIWPESTKHAHESAFWLSVKGDSMTSP
SGISFPEGMIILVDPEKEPMPGNFVIAKLTDDNEATFKKLVVDAGIKYLKPLNPAYRLIELNGNCKILGVVVDARWLQID

Specific function: May control the expression of the integrase and inhibit excision of the mobile element R391, and regulate the expression of other genes as well [H]

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787391, Length=139, Percent_Identity=38.8489208633094, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056 [H]

Pfam domain/function: PF01381 HTH_3; PF00717 Peptidase_S24 [H]

EC number: NA

Molecular weight: Translated: 26279; Mature: 26279

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKPLTPEQLDDAKRLKELFNAKKKALGISQESVAHELGVGQSAVNQFLNGVNPLNITN
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCCH
AAAFAKVLNEPISSFSPSLAKELAKMAESLSITTRSGAKDKPAVSVANSYPLISWISAGN
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEEECCCCCCCCCEEECCCCCEEEEECCCC
WYEAIEPYTLRDIEIWPESTKHAHESAFWLSVKGDSMTSPSGISFPEGMIILVDPEKEPM
HHHHCCCEEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCC
PGNFVIAKLTDDNEATFKKLVVDAGIKYLKPLNPAYRLIELNGNCKILGVVVDARWLQID
CCCEEEEEEECCCHHHHHHHHHHHCHHHHCCCCCCEEEEEECCCEEEEEEEEEEEEEEEC
>Mature Secondary Structure
MKKKPLTPEQLDDAKRLKELFNAKKKALGISQESVAHELGVGQSAVNQFLNGVNPLNITN
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCCH
AAAFAKVLNEPISSFSPSLAKELAKMAESLSITTRSGAKDKPAVSVANSYPLISWISAGN
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEEECCCCCCCCCEEECCCCCEEEEECCCC
WYEAIEPYTLRDIEIWPESTKHAHESAFWLSVKGDSMTSPSGISFPEGMIILVDPEKEPM
HHHHCCCEEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCC
PGNFVIAKLTDDNEATFKKLVVDAGIKYLKPLNPAYRLIELNGNCKILGVVVDARWLQID
CCCEEEEEEECCCHHHHHHHHHHHCHHHHCCCCCCEEEEEECCCEEEEEEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11157923; 12193633 [H]