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The map label for this gene is grpE [H]

Identifier: 218928275

GI number: 218928275

Start: 1250712

End: 1251290

Strand: Direct

Name: grpE [H]

Synonym: YPO1107

Alternate gene names: 218928275

Gene position: 1250712-1251290 (Clockwise)

Preceding gene: 218928271

Following gene: 218928277

Centisome position: 26.88

GC content: 47.32

Gene sequence:

>579_bases
ATGAGTAGTAAAGAACAGAAAACGCCAAACGAGCAAGTCTCGGAAGAAATGGAAAATACAGCAGAGCAGCAAGTGGAAGC
AACACAAGAGACGGGGGAATGTGTAGATCCGCGTGTTGCAGAGCTTGAGGTGCAGTTGTCTGATGCTCTACAGCGTGAGC
GCGAAAGCCTGTTACGCGCTAAGGCTGAAGTTGAGAATATCCGTCGTCGTACTGAATTGGATGTCGAGAAAGCGCATAAA
TTTGCACTGGAGAGATTCTCATCTGAATTATTGCCAGTGATTGACAATCTGGAGCGCGCTCTAGATACCGCTGATAAGAC
CAATACTGAACTGATCTCAATGATTGAAGGTGTCGAACTGACACTGAAATCACTGCTAGATGCTGTCGGTAAATTTGGTA
TTGAAGTTGTCGGCGAGACCCATGTGCCGTTTAACCCTGAGGTGCATCAGGCCATGACAATGCTTGAGTCTGCTGATCAC
GAGCCAAACCACGTAATGATGGTGATGCAAAAAGGCTATACGCTGAATGGCCGGCTGTTACGCCCAGCAATGGTTGCGGT
ATCTAAAGCGAAATCGTAA

Upstream 100 bases:

>100_bases
TGTCTTTTACCCCACATTATGACCGGTTCCCTTGAATCCCCGGTTTTGATCCCCATAATAAGCCAAGTAGCGAGATTAAT
GCTAAAACGCGGAGATATCC

Downstream 100 bases:

>100_bases
AGTGCTCTAACGTTAAAAGGCACCTCAACGGTGCCTCAAATTGCTAACAAAGTCCTGGCCTCCTAGCCGGGACTTTGCTC
TAATTTTACGCGGTAACGCG

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 192; Mature: 191

Protein sequence:

>192_residues
MSSKEQKTPNEQVSEEMENTAEQQVEATQETGECVDPRVAELEVQLSDALQRERESLLRAKAEVENIRRRTELDVEKAHK
FALERFSSELLPVIDNLERALDTADKTNTELISMIEGVELTLKSLLDAVGKFGIEVVGETHVPFNPEVHQAMTMLESADH
EPNHVMMVMQKGYTLNGRLLRPAMVAVSKAKS

Sequences:

>Translated_192_residues
MSSKEQKTPNEQVSEEMENTAEQQVEATQETGECVDPRVAELEVQLSDALQRERESLLRAKAEVENIRRRTELDVEKAHK
FALERFSSELLPVIDNLERALDTADKTNTELISMIEGVELTLKSLLDAVGKFGIEVVGETHVPFNPEVHQAMTMLESADH
EPNHVMMVMQKGYTLNGRLLRPAMVAVSKAKS
>Mature_191_residues
SSKEQKTPNEQVSEEMENTAEQQVEATQETGECVDPRVAELEVQLSDALQRERESLLRAKAEVENIRRRTELDVEKAHKF
ALERFSSELLPVIDNLERALDTADKTNTELISMIEGVELTLKSLLDAVGKFGIEVVGETHVPFNPEVHQAMTMLESADHE
PNHVMMVMQKGYTLNGRLLRPAMVAVSKAKS

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=195, Percent_Identity=30.7692307692308, Blast_Score=80, Evalue=1e-15,
Organism=Escherichia coli, GI1788967, Length=197, Percent_Identity=59.8984771573604, Blast_Score=236, Evalue=6e-64,
Organism=Caenorhabditis elegans, GI17552458, Length=151, Percent_Identity=32.4503311258278, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324806, Length=160, Percent_Identity=35, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24653432, Length=181, Percent_Identity=32.0441988950276, Blast_Score=85, Evalue=3e-17,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 21558; Mature: 21427

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKEQKTPNEQVSEEMENTAEQQVEATQETGECVDPRVAELEVQLSDALQRERESLLRA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KAEVENIRRRTELDVEKAHKFALERFSSELLPVIDNLERALDTADKTNTELISMIEGVEL
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
TLKSLLDAVGKFGIEVVGETHVPFNPEVHQAMTMLESADHEPNHVMMVMQKGYTLNGRLL
HHHHHHHHHHHHCHHEECCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHH
RPAMVAVSKAKS
HHHHHHHHHCCC
>Mature Secondary Structure 
SSKEQKTPNEQVSEEMENTAEQQVEATQETGECVDPRVAELEVQLSDALQRERESLLRA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KAEVENIRRRTELDVEKAHKFALERFSSELLPVIDNLERALDTADKTNTELISMIEGVEL
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
TLKSLLDAVGKFGIEVVGETHVPFNPEVHQAMTMLESADHEPNHVMMVMQKGYTLNGRLL
HHHHHHHHHHHHCHHEECCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHH
RPAMVAVSKAKS
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA