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The map label for this gene is tsf [H]

Identifier: 218928213

GI number: 218928213

Start: 1186217

End: 1187074

Strand: Direct

Name: tsf [H]

Synonym: YPO1045

Alternate gene names: 218928213

Gene position: 1186217-1187074 (Clockwise)

Preceding gene: 218928212

Following gene: 218928214

Centisome position: 25.49

GC content: 48.02

Gene sequence:

>858_bases
ATGGTTGCTATTACCGCTGCTTTGGTAAAAGAACTGCGTGAGCGTACAGCCGCAGGCATGATGGAATGTAAAAAGGCGTT
GGTTGAAGCTAATGGTGACATTGAGCTGGCCATCGACAACATGCGTAAGTCTGGTCAGGCTAAAGCTGCCAAGAAAGCAG
GCCGTATTGCTGCTGAAGGTATCATCCTGGCTAAAGTTTCCGCTGATGGTAAGTACGGTGTGATTCTGGAACTGAACTGC
GAAACTGACTTCGTTGCTAAAGATGCTGGCTTCAAAGCATTCGGCGAAGAAGTTATCAACGCAGCTCTGGCTGAAAAAAT
CGCTGATATCGATGTTCTGAAAGCTAAGTTCGAAGAACAACGTGCAAACCTGGTCGCTAAAATCGGTGAGAACATCAACA
TTCGCCGTGTCGCTGTACTGGAAGGCGACATTCTGGGTACTTACCTGCATGGCGCACGTATCGGTGTTATGGTTGCGGCC
ACGGGTGCTGATGAAGAACTGGTTAAGCACATTGCTATGCACATCGCTGCAAGCAAGCCTGAATATGTTAAGCCAGACGA
TGTTCCTGCTGAAGTTGTTGCTCGCGAGCACCAAATCCAGTTGGACATTGCTATCGAATCCGGCAAGCCACGTGAGATTG
CTGAGAAGATGGTTGAAGGCCGTATGCGTAAGTTCACTGGCGAAGTCTCTCTGACTGGTCAGAACTTCGTTATGGACCCA
AGCAAAACTGTTGGCGATCTGTTGAAAGAAAACAACGCTGATGTCGTGAACTTCATCCGCTTTGAAGTGGGCGAAGGCAT
TGAGAAAGTTGAAACTGACTTTGCTGCTGAAGTTGCAGCAATGAGCAAACAGTCTTAA

Upstream 100 bases:

>100_bases
TAACCAGGTATTGAAATATGTTGGTTAGGGGGGCCTTATAGGCCCCCTTTGCATATCTAATGTGAGATTTTGTCTTCACA
AGAGAACCGAGGAAATTTAA

Downstream 100 bases:

>100_bases
TGCTCCAAATGGGGCCGCCAATAGGCGGTTCCATTTTATTCACTCGATACCCGTGATACTTCAAGTTGCAAGCGCGTTGA
CTGCCTTCCTATAACTCGAA

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MVAITAALVKELRERTAAGMMECKKALVEANGDIELAIDNMRKSGQAKAAKKAGRIAAEGIILAKVSADGKYGVILELNC
ETDFVAKDAGFKAFGEEVINAALAEKIADIDVLKAKFEEQRANLVAKIGENINIRRVAVLEGDILGTYLHGARIGVMVAA
TGADEELVKHIAMHIAASKPEYVKPDDVPAEVVAREHQIQLDIAIESGKPREIAEKMVEGRMRKFTGEVSLTGQNFVMDP
SKTVGDLLKENNADVVNFIRFEVGEGIEKVETDFAAEVAAMSKQS

Sequences:

>Translated_285_residues
MVAITAALVKELRERTAAGMMECKKALVEANGDIELAIDNMRKSGQAKAAKKAGRIAAEGIILAKVSADGKYGVILELNC
ETDFVAKDAGFKAFGEEVINAALAEKIADIDVLKAKFEEQRANLVAKIGENINIRRVAVLEGDILGTYLHGARIGVMVAA
TGADEELVKHIAMHIAASKPEYVKPDDVPAEVVAREHQIQLDIAIESGKPREIAEKMVEGRMRKFTGEVSLTGQNFVMDP
SKTVGDLLKENNADVVNFIRFEVGEGIEKVETDFAAEVAAMSKQS
>Mature_285_residues
MVAITAALVKELRERTAAGMMECKKALVEANGDIELAIDNMRKSGQAKAAKKAGRIAAEGIILAKVSADGKYGVILELNC
ETDFVAKDAGFKAFGEEVINAALAEKIADIDVLKAKFEEQRANLVAKIGENINIRRVAVLEGDILGTYLHGARIGVMVAA
TGADEELVKHIAMHIAASKPEYVKPDDVPAEVVAREHQIQLDIAIESGKPREIAEKMVEGRMRKFTGEVSLTGQNFVMDP
SKTVGDLLKENNADVVNFIRFEVGEGIEKVETDFAAEVAAMSKQS

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome [H]

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family [H]

Homologues:

Organism=Homo sapiens, GI171846268, Length=289, Percent_Identity=25.6055363321799, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI1786366, Length=285, Percent_Identity=77.8947368421053, Blast_Score=455, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI17561440, Length=295, Percent_Identity=26.7796610169492, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19921466, Length=287, Percent_Identity=30.3135888501742, Blast_Score=95, Evalue=4e-20,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449 [H]

Pfam domain/function: PF00889 EF_TS; PF00627 UBA [H]

EC number: NA

Molecular weight: Translated: 30722; Mature: 30722

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAITAALVKELRERTAAGMMECKKALVEANGDIELAIDNMRKSGQAKAAKKAGRIAAEG
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCHHHCC
IILAKVSADGKYGVILELNCETDFVAKDAGFKAFGEEVINAALAEKIADIDVLKAKFEEQ
EEEEEEECCCCEEEEEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RANLVAKIGENINIRRVAVLEGDILGTYLHGARIGVMVAATGADEELVKHIAMHIAASKP
HHHHHHHHCCCCCEEEEEEEECCHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHCCCC
EYVKPDDVPAEVVAREHQIQLDIAIESGKPREIAEKMVEGRMRKFTGEVSLTGQNFVMDP
CCCCCCCCCHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEECCCCEEECC
SKTVGDLLKENNADVVNFIRFEVGEGIEKVETDFAAEVAAMSKQS
HHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVAITAALVKELRERTAAGMMECKKALVEANGDIELAIDNMRKSGQAKAAKKAGRIAAEG
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCHHHCC
IILAKVSADGKYGVILELNCETDFVAKDAGFKAFGEEVINAALAEKIADIDVLKAKFEEQ
EEEEEEECCCCEEEEEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RANLVAKIGENINIRRVAVLEGDILGTYLHGARIGVMVAATGADEELVKHIAMHIAASKP
HHHHHHHHCCCCCEEEEEEEECCHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHCCCC
EYVKPDDVPAEVVAREHQIQLDIAIESGKPREIAEKMVEGRMRKFTGEVSLTGQNFVMDP
CCCCCCCCCHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEECCCCEEECC
SKTVGDLLKENNADVVNFIRFEVGEGIEKVETDFAAEVAAMSKQS
HHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA