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The map label for this gene is truC

Identifier: 218928206

GI number: 218928206

Start: 1178545

End: 1179318

Strand: Direct

Name: truC

Synonym: YPO1038

Alternate gene names: 218928206

Gene position: 1178545-1179318 (Clockwise)

Preceding gene: 218928205

Following gene: 218928207

Centisome position: 25.32

GC content: 48.71

Gene sequence:

>774_bases
ATGCTGGAAATTATCTATCAAGATGAACACATTGTTGCGGTGAATAAGCCTGCCGGTTGGTTAGTGCATCGTAGTTGGTT
GGACCGCAATGAAACGGTATTTGTTATGCAAACGGTACGTGATCAGATTGGTCAGCATGTGTATACCGTTCATCGCCTTG
ATCGCCCGACATCAGGCGTATTGCTCATGGCATTGTCCAGTGATGTGGCTCGTATGTTGTCGTTACAATTTGAGCAACAC
CAGATTCAGAAAACGTATCATGCGGTGGTGCGCGGATATGTATTGGAAGGTGGCACCGTTGATTACGCGATGGCCGAAGA
GCTGGATAAAATAGCGGATAAGTTTGCTAAGTCTGATAAGGCTCCACAGCCTTCTGTCAGCCATTATGAAGCATTAGCGC
AAGTGGAAGTTCCTTTGGCGATTGGCCGCTATGAAACTGCTCGCTATAGCTTGGTGGCATTGAAACCGGAAACGGGTCGT
AAGCACCAGTTGCGTCGGCATATGGCTCATATCCGTCACCCCATTATTGGTGACAGTACACATGGTGATTTACGTCAGAA
CAGAGGTGTCGCTCAGCATTTTGGTTGTTCACGGTTGATGCTGCATGCCAGCCATTTACACCTCAATCACCCTGTGACCG
GTGAAGCGCTGACGCTTACCGCTCGTTGGGATGAACCCTGGCAAGGGTTGATGTCACAATTTGGCTGGTCGGGTATTGCC
CCTCATCTTGAAAGGGTTGAGTTTCCGCTGACTGCCAGTCAGGATAATGAATAA

Upstream 100 bases:

>100_bases
ATTTTGAAGAGGCTTTGAGCGGTGATGGGCGGCCAGATTACTCGGCGCTTTTGGCACAATTACGGTGCCTTGATGATCTG
TTGAATAAAGAAAGTGAATA

Downstream 100 bases:

>100_bases
TTTTTTGGCTACAAAGGAATATCTTTTATGGCTCAAGTTGGGATTTTTGTTGGTACTGTCTATGGTAATGCGTTGTTAGT
GGCAGAAGAAGCTGAGAACA

Product: tRNA pseudouridine synthase C

Products: NA

Alternate protein names: tRNA pseudouridylate synthase C; tRNA-uridine isomerase C

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MLEIIYQDEHIVAVNKPAGWLVHRSWLDRNETVFVMQTVRDQIGQHVYTVHRLDRPTSGVLLMALSSDVARMLSLQFEQH
QIQKTYHAVVRGYVLEGGTVDYAMAEELDKIADKFAKSDKAPQPSVSHYEALAQVEVPLAIGRYETARYSLVALKPETGR
KHQLRRHMAHIRHPIIGDSTHGDLRQNRGVAQHFGCSRLMLHASHLHLNHPVTGEALTLTARWDEPWQGLMSQFGWSGIA
PHLERVEFPLTASQDNE

Sequences:

>Translated_257_residues
MLEIIYQDEHIVAVNKPAGWLVHRSWLDRNETVFVMQTVRDQIGQHVYTVHRLDRPTSGVLLMALSSDVARMLSLQFEQH
QIQKTYHAVVRGYVLEGGTVDYAMAEELDKIADKFAKSDKAPQPSVSHYEALAQVEVPLAIGRYETARYSLVALKPETGR
KHQLRRHMAHIRHPIIGDSTHGDLRQNRGVAQHFGCSRLMLHASHLHLNHPVTGEALTLTARWDEPWQGLMSQFGWSGIA
PHLERVEFPLTASQDNE
>Mature_257_residues
MLEIIYQDEHIVAVNKPAGWLVHRSWLDRNETVFVMQTVRDQIGQHVYTVHRLDRPTSGVLLMALSSDVARMLSLQFEQH
QIQKTYHAVVRGYVLEGGTVDYAMAEELDKIADKFAKSDKAPQPSVSHYEALAQVEVPLAIGRYETARYSLVALKPETGR
KHQLRRHMAHIRHPIIGDSTHGDLRQNRGVAQHFGCSRLMLHASHLHLNHPVTGEALTLTARWDEPWQGLMSQFGWSGIA
PHLERVEFPLTASQDNE

Specific function: Responsible for synthesis of pseudouridine from uracil- 65 in transfer RNAs

COG id: COG0564

COG function: function code J; Pseudouridylate synthases, 23S RNA-specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rluA family

Homologues:

Organism=Homo sapiens, GI17158025, Length=226, Percent_Identity=28.3185840707965, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI23308689, Length=185, Percent_Identity=27.027027027027, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221316667, Length=186, Percent_Identity=29.5698924731183, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI221316663, Length=193, Percent_Identity=29.0155440414508, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1789155, Length=257, Percent_Identity=69.2607003891051, Blast_Score=380, Evalue=1e-107,
Organism=Escherichia coli, GI1787327, Length=233, Percent_Identity=32.1888412017167, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1786244, Length=224, Percent_Identity=35.2678571428571, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788946, Length=230, Percent_Identity=31.304347826087, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324506, Length=186, Percent_Identity=26.8817204301075, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320168, Length=183, Percent_Identity=26.775956284153, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321608, Length=197, Percent_Identity=27.9187817258883, Blast_Score=69, Evalue=5e-13,
Organism=Drosophila melanogaster, GI19921090, Length=180, Percent_Identity=32.2222222222222, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24583449, Length=178, Percent_Identity=32.0224719101124, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24583447, Length=178, Percent_Identity=32.0224719101124, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24583445, Length=178, Percent_Identity=32.0224719101124, Blast_Score=83, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUC_YERPE (Q8ZH72)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AE0127
- RefSeq:   NP_670442.1
- RefSeq:   NP_994120.1
- RefSeq:   YP_002346081.1
- ProteinModelPortal:   Q8ZH72
- SMR:   Q8ZH72
- GeneID:   1148090
- GeneID:   1173878
- GeneID:   2764549
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO1038
- KEGG:   ypk:y3143
- KEGG:   ypm:YP_2813
- HOGENOM:   HBG750656
- OMA:   MPVAIGR
- ProtClustDB:   PRK11112
- BioCyc:   YPES187410:Y3143-MONOMER
- BioCyc:   YPES214092:YPO1038-MONOMER
- InterPro:   IPR020103
- InterPro:   IPR006224
- InterPro:   IPR006145

Pfam domain/function: PF00849 PseudoU_synth_2; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 29213; Mature: 29213

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS01129 PSI_RLU

Important sites: ACT_SITE 54-54

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEIIYQDEHIVAVNKPAGWLVHRSWLDRNETVFVMQTVRDQIGQHVYTVHRLDRPTSGV
CEEEEEECCEEEEEECCCCCEEEHHHCCCCCEEEEHHHHHHHHHHHEEHHHHCCCCCCCE
LLMALSSDVARMLSLQFEQHQIQKTYHAVVRGYVLEGGTVDYAMAEELDKIADKFAKSDK
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHCCC
APQPSVSHYEALAQVEVPLAIGRYETARYSLVALKPETGRKHQLRRHMAHIRHPIIGDST
CCCCCHHHHHHHHHEECCEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCC
HGDLRQNRGVAQHFGCSRLMLHASHLHLNHPVTGEALTLTARWDEPWQGLMSQFGWSGIA
CCHHHHCCCHHHHCCHHHHHHHHHHEEECCCCCCCEEEEEECCCCHHHHHHHHCCCCCCC
PHLERVEFPLTASQDNE
CHHHHEECCCCCCCCCC
>Mature Secondary Structure
MLEIIYQDEHIVAVNKPAGWLVHRSWLDRNETVFVMQTVRDQIGQHVYTVHRLDRPTSGV
CEEEEEECCEEEEEECCCCCEEEHHHCCCCCEEEEHHHHHHHHHHHEEHHHHCCCCCCCE
LLMALSSDVARMLSLQFEQHQIQKTYHAVVRGYVLEGGTVDYAMAEELDKIADKFAKSDK
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHCCC
APQPSVSHYEALAQVEVPLAIGRYETARYSLVALKPETGRKHQLRRHMAHIRHPIIGDST
CCCCCHHHHHHHHHEECCEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCC
HGDLRQNRGVAQHFGCSRLMLHASHLHLNHPVTGEALTLTARWDEPWQGLMSQFGWSGIA
CCHHHHCCCHHHHCCHHHHHHHHHHEEECCCCCCCEEEEEECCCCHHHHHHHHCCCCCCC
PHLERVEFPLTASQDNE
CHHHHEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430