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The map label for this gene is gcvA [H]

Identifier: 218928197

GI number: 218928197

Start: 1169923

End: 1170840

Strand: Direct

Name: gcvA [H]

Synonym: YPO1029

Alternate gene names: 218928197

Gene position: 1169923-1170840 (Clockwise)

Preceding gene: 218928194

Following gene: 218928198

Centisome position: 25.14

GC content: 49.13

Gene sequence:

>918_bases
ATGTCAAAACGATTACCACCCCTGAATGCCTTACGGGCTTTTGATGCCGCCGCCCGTCACCTCAGTTTCACTAAAGCAGC
CGAGGAGTTATTTGTCACTCAAGCCGCTGTTAGCCACCAGATCAAATCGCTGGAGGATTTTCTCGGGCTGAAATTGTTCC
GCCGACGTAATCGCTCTTTGTTGTTGACCGAAGAAGGGCAGAGCTATTACCTCGATATCAAAGAGATTTTCACGTCTATT
AATGAAGCGACTCGTAAGTTGCAAGCGCGCAGTGCGAAGGGAGCGTTAACCGTCAGTTTGCCGCCCAGTTTTGCCATTCA
ATGGCTAGTTCCTCGTTTGTCTGGGTTTAATGCGGCTTATCCAGGCATTGATGTGAGGATCCAAGCGGTGGATCGGGAAG
AGGATAAGCTCGCTGATGATGTGGATGTCGCGATATTCTACGGCCGCGGTAATTGGAGTGGGTTGCGTACAGAGCGCTTG
TATGCTGAATTCCTATTACCCGTCTGTGCACCTAGCTTACTAACTGGTGAGAATGGATTAAAAGTACCATCAGATCTGGC
TAATCACACCTTGTTGCATGATACTTCACGCCGTGACTGGTTAGCGTATACCCGCCAACTGGGGGTACCGCAGATTAATG
TGCAGCAAGGCCCGATATTTAGCCACAGTGCCATGGTGGTTCAGGCTGCGGTTCATGGTCAAGGGATTGCTCTGGTGAAT
AATGTCATGGCCCAGTCTGAGATTGAAGCGGGCCGATTGGTTTGCCCGTTTAACGATGTATTGGTGAGTAAGAATGCTTT
TTATTTGGTTTGTCATGACAGTCAGGCAGAACTGGGTAAAATAGCCGCCTTCCGTCAGTGGATACTGGCAAGAGCTGCCA
GCGAGCAGGAGAAATTACGTTTTCGTTATGAAAACTGA

Upstream 100 bases:

>100_bases
ATCCTCAATGGACAATTTATAATGGCTCGGATTATAAAAACTAATAAGTAAACAAAGGGTTTCATCTGATGATGGGCCGT
TATAAAAAAGTGTCCAATTC

Downstream 100 bases:

>100_bases
TCAATCATAATGTTTGGTTAATTGTAATGCTTGGTTAATCACTGCACTTGATTGGTTACAGTATCTGCGCAGTAATGACA
ACCCATTCATGATGGTAACC

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MSKRLPPLNALRAFDAAARHLSFTKAAEELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSLLLTEEGQSYYLDIKEIFTSI
NEATRKLQARSAKGALTVSLPPSFAIQWLVPRLSGFNAAYPGIDVRIQAVDREEDKLADDVDVAIFYGRGNWSGLRTERL
YAEFLLPVCAPSLLTGENGLKVPSDLANHTLLHDTSRRDWLAYTRQLGVPQINVQQGPIFSHSAMVVQAAVHGQGIALVN
NVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQAELGKIAAFRQWILARAASEQEKLRFRYEN

Sequences:

>Translated_305_residues
MSKRLPPLNALRAFDAAARHLSFTKAAEELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSLLLTEEGQSYYLDIKEIFTSI
NEATRKLQARSAKGALTVSLPPSFAIQWLVPRLSGFNAAYPGIDVRIQAVDREEDKLADDVDVAIFYGRGNWSGLRTERL
YAEFLLPVCAPSLLTGENGLKVPSDLANHTLLHDTSRRDWLAYTRQLGVPQINVQQGPIFSHSAMVVQAAVHGQGIALVN
NVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQAELGKIAAFRQWILARAASEQEKLRFRYEN
>Mature_304_residues
SKRLPPLNALRAFDAAARHLSFTKAAEELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSLLLTEEGQSYYLDIKEIFTSIN
EATRKLQARSAKGALTVSLPPSFAIQWLVPRLSGFNAAYPGIDVRIQAVDREEDKLADDVDVAIFYGRGNWSGLRTERLY
AEFLLPVCAPSLLTGENGLKVPSDLANHTLLHDTSRRDWLAYTRQLGVPQINVQQGPIFSHSAMVVQAAVHGQGIALVNN
VMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQAELGKIAAFRQWILARAASEQEKLRFRYEN

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=305, Percent_Identity=87.8688524590164, Blast_Score=561, Evalue=1e-161,
Organism=Escherichia coli, GI1786448, Length=286, Percent_Identity=34.6153846153846, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1788706, Length=288, Percent_Identity=30.9027777777778, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI145693193, Length=296, Percent_Identity=27.027027027027, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1786401, Length=274, Percent_Identity=27.7372262773723, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789639, Length=250, Percent_Identity=28.4, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI157672245, Length=162, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87081978, Length=261, Percent_Identity=28.735632183908, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787128, Length=304, Percent_Identity=23.6842105263158, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI145693105, Length=122, Percent_Identity=31.1475409836066, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1790262, Length=257, Percent_Identity=25.6809338521401, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34082; Mature: 33951

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRLPPLNALRAFDAAARHLSFTKAAEELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LLTEEGQSYYLDIKEIFTSINEATRKLQARSAKGALTVSLPPSFAIQWLVPRLSGFNAAY
EEEECCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCCC
PGIDVRIQAVDREEDKLADDVDVAIFYGRGNWSGLRTERLYAEFLLPVCAPSLLTGENGL
CCCEEEEEEECCCHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCHHEECCCCC
KVPSDLANHTLLHDTSRRDWLAYTRQLGVPQINVQQGPIFSHSAMVVQAAVHGQGIALVN
CCCHHHHCCEEECCCCCHHHHHHHHHCCCCEEECCCCCCCCCCHHHEEHHHCCCCHHHHH
NVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQAELGKIAAFRQWILARAASEQEKLR
HHHHHHHCCCCCEECCCHHHEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHE
FRYEN
EECCC
>Mature Secondary Structure 
SKRLPPLNALRAFDAAARHLSFTKAAEELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LLTEEGQSYYLDIKEIFTSINEATRKLQARSAKGALTVSLPPSFAIQWLVPRLSGFNAAY
EEEECCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCCC
PGIDVRIQAVDREEDKLADDVDVAIFYGRGNWSGLRTERLYAEFLLPVCAPSLLTGENGL
CCCEEEEEEECCCHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCHHEECCCCC
KVPSDLANHTLLHDTSRRDWLAYTRQLGVPQINVQQGPIFSHSAMVVQAAVHGQGIALVN
CCCHHHHCCEEECCCCCHHHHHHHHHCCCCEEECCCCCCCCCCHHHEEHHHCCCCHHHHH
NVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQAELGKIAAFRQWILARAASEQEKLR
HHHHHHHCCCCCEECCCHHHEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHE
FRYEN
EECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]