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The map label for this gene is 218928110

Identifier: 218928110

GI number: 218928110

Start: 1029436

End: 1030320

Strand: Direct

Name: 218928110

Synonym: YPO0938

Alternate gene names: NA

Gene position: 1029436-1030320 (Clockwise)

Preceding gene: 218928109

Following gene: 218928111

Centisome position: 22.12

GC content: 50.4

Gene sequence:

>885_bases
ATGACAAAAGTGACTGTTGCTGCGACTCAAATGGCCTGTTCTTGGGACCTGCCGAAAAACATTGAAAATGCCGAAAAATT
GGTACGACAGGCACATGCCAAAGGCGCACAGATCATCCTGATTCAGGAGCTGTTTGCCGCACCTTACTTCTGCATTGATC
AAAGCCCAGAGCACTACGCACTGGCCCAAGAGCTGGATAATAGTCCATTAATTAAACACTTCTCCAAGCTTGCCGCTGAG
TTGGAAGTGGTACTACCGCTGAGTTTCTTTGAGAAAGCCAATAATGCTTACTATAACTCGCTAGTCATGATTGACGCCGA
TGGCTCGGTGCTGGACGTCTACCGTAAAACCCACATTCCTAATGGCCCGGCTTATCAGGAGAAACAGTTCTTTATCCCTG
GCGATACCGGTTTTAAAGTTTGGCAAACCCGTTATGCCAAAGTGGGCGTAGGCATCTGTTGGGATCAGTGGTTCCCTGAA
ACCGCGCGCAGCCTGGCACTGCTTGGGGCAGAAGTTATCTTCTACCCAACAGCAATCGGCTCAGAACCTGCTTACCCTGA
AATTGACAGCCAACCGCACTGGACCCGTGTCCAGCAAGGCCATGCAGCCGCAAACCTGGTTCCAGTGATTGCCTCTAACC
GCATCGGTACTGAGAAAAGTAAATACATTGATGGGCTGGAGATGACGTTCTACGGCTCCTCGTTTATTGCTGACCAAACT
GGCGCACTGTTAGCTCAAGCTAACAAAACTGACGAAGCGGTTCTGGTGCATGAATTTGATTTGCAGGAGATCGCCGCCCA
ACGTGCTTCATGGGGCTTGTTCCGTGATCGCCGCCCAGAGATGTACCAAACATTAGCCACGTCAGACGGCAAGACCCGGA
GATAA

Upstream 100 bases:

>100_bases
TGTATACTTATAACTTTGTATACTGCGGCGATGCCAATCAGCAGTTTAAAATATTGCACACCTCTTGCAGGTGTTTTTTT
ACATTTATTGAGGTTTTCTC

Downstream 100 bases:

>100_bases
TTTATGTCGGTGCAAGACACGATGTTACAACAACAGGCATTACCCGGCACACCGCGGCAGGATGGTTTCTTTATGCCTGC
AGAATGGGCACCGCAGGATG

Product: putative carbon-nitrogen hydrolase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MTKVTVAATQMACSWDLPKNIENAEKLVRQAHAKGAQIILIQELFAAPYFCIDQSPEHYALAQELDNSPLIKHFSKLAAE
LEVVLPLSFFEKANNAYYNSLVMIDADGSVLDVYRKTHIPNGPAYQEKQFFIPGDTGFKVWQTRYAKVGVGICWDQWFPE
TARSLALLGAEVIFYPTAIGSEPAYPEIDSQPHWTRVQQGHAAANLVPVIASNRIGTEKSKYIDGLEMTFYGSSFIADQT
GALLAQANKTDEAVLVHEFDLQEIAAQRASWGLFRDRRPEMYQTLATSDGKTRR

Sequences:

>Translated_294_residues
MTKVTVAATQMACSWDLPKNIENAEKLVRQAHAKGAQIILIQELFAAPYFCIDQSPEHYALAQELDNSPLIKHFSKLAAE
LEVVLPLSFFEKANNAYYNSLVMIDADGSVLDVYRKTHIPNGPAYQEKQFFIPGDTGFKVWQTRYAKVGVGICWDQWFPE
TARSLALLGAEVIFYPTAIGSEPAYPEIDSQPHWTRVQQGHAAANLVPVIASNRIGTEKSKYIDGLEMTFYGSSFIADQT
GALLAQANKTDEAVLVHEFDLQEIAAQRASWGLFRDRRPEMYQTLATSDGKTRR
>Mature_293_residues
TKVTVAATQMACSWDLPKNIENAEKLVRQAHAKGAQIILIQELFAAPYFCIDQSPEHYALAQELDNSPLIKHFSKLAAEL
EVVLPLSFFEKANNAYYNSLVMIDADGSVLDVYRKTHIPNGPAYQEKQFFIPGDTGFKVWQTRYAKVGVGICWDQWFPET
ARSLALLGAEVIFYPTAIGSEPAYPEIDSQPHWTRVQQGHAAANLVPVIASNRIGTEKSKYIDGLEMTFYGSSFIADQTG
ALLAQANKTDEAVLVHEFDLQEIAAQRASWGLFRDRRPEMYQTLATSDGKTRR

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=290, Percent_Identity=28.6206896551724, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI5031947, Length=292, Percent_Identity=27.7397260273973, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI297632348, Length=292, Percent_Identity=27.7397260273973, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI297632350, Length=292, Percent_Identity=27.7397260273973, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI7706509, Length=270, Percent_Identity=27.4074074074074, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI297632346, Length=203, Percent_Identity=30.0492610837438, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17533173, Length=270, Percent_Identity=28.8888888888889, Blast_Score=120, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17556280, Length=286, Percent_Identity=24.8251748251748, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323383, Length=291, Percent_Identity=26.8041237113402, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6322335, Length=306, Percent_Identity=27.1241830065359, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21358471, Length=270, Percent_Identity=28.5185185185185, Blast_Score=117, Evalue=9e-27,
Organism=Drosophila melanogaster, GI21355835, Length=288, Percent_Identity=23.9583333333333, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17933642, Length=283, Percent_Identity=23.6749116607774, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 32881; Mature: 32749

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKVTVAATQMACSWDLPKNIENAEKLVRQAHAKGAQIILIQELFAAPYFCIDQSPEHYA
CCEEEEEEEHEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEHHHCCCEEEECCCCCHHH
LAQELDNSPLIKHFSKLAAELEVVLPLSFFEKANNAYYNSLVMIDADGSVLDVYRKTHIP
HHHHCCCCHHHHHHHHHHHHHHHEECHHHHHHCCCCEEEEEEEEECCCCEEEHHHHHCCC
NGPAYQEKQFFIPGDTGFKVWQTRYAKVGVGICWDQWFPETARSLALLGAEVIFYPTAIG
CCCCCCCCEEEECCCCCHHHHHHHHHHHCCEEEHHHCCHHHHHHHHHHCCEEEEEEECCC
SEPAYPEIDSQPHWTRVQQGHAAANLVPVIASNRIGTEKSKYIDGLEMTFYGSSFIADQT
CCCCCCCCCCCCCHHHHHCCCCHHHEEEEEECCCCCCCHHHHHCCEEEEEECCCHHCCCC
GALLAQANKTDEAVLVHEFDLQEIAAQRASWGLFRDRRPEMYQTLATSDGKTRR
CHHEEECCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TKVTVAATQMACSWDLPKNIENAEKLVRQAHAKGAQIILIQELFAAPYFCIDQSPEHYA
CEEEEEEEHEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEHHHCCCEEEECCCCCHHH
LAQELDNSPLIKHFSKLAAELEVVLPLSFFEKANNAYYNSLVMIDADGSVLDVYRKTHIP
HHHHCCCCHHHHHHHHHHHHHHHEECHHHHHHCCCCEEEEEEEEECCCCEEEHHHHHCCC
NGPAYQEKQFFIPGDTGFKVWQTRYAKVGVGICWDQWFPETARSLALLGAEVIFYPTAIG
CCCCCCCCEEEECCCCCHHHHHHHHHHHCCEEEHHHCCHHHHHHHHHHCCEEEEEEECCC
SEPAYPEIDSQPHWTRVQQGHAAANLVPVIASNRIGTEKSKYIDGLEMTFYGSSFIADQT
CCCCCCCCCCCCCHHHHHCCCCHHHEEEEEECCCCCCCHHHHHCCEEEEEECCCHHCCCC
GALLAQANKTDEAVLVHEFDLQEIAAQRASWGLFRDRRPEMYQTLATSDGKTRR
CHHEEECCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]