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The map label for this gene is gshB

Identifier: 218928107

GI number: 218928107

Start: 1027196

End: 1028155

Strand: Direct

Name: gshB

Synonym: YPO0935

Alternate gene names: 218928107

Gene position: 1027196-1028155 (Clockwise)

Preceding gene: 218928106

Following gene: 218928108

Centisome position: 22.07

GC content: 49.17

Gene sequence:

>960_bases
ATGATTAAGCTCGGTATCGTAATGGACCCTATTTCGTCCATTAACATCAAGAAAGACACCAGTTTCGCTATGCTATTAGA
AGCGCAGCGCCGTGGCTGGGAATTGCATTACATGGAGATGGGGGATCTCTATATGCGTGGGGGTGATGGCCGCGCACGTA
CCCGCCGACTGAATGTGAAGCAGGATAAAGATAACTGGTTCAGTTTTGGGGCTGAGCAGGACCTGCCGCTCTATGATCTT
GATGTCATTCTGATGCGTAAAGATCCGCCGTTTGATACCGAGTTTATCTATGCCACCTATATTCTGGAACGTGCAGAAGA
CAAAGGCACACTGGTGGTGAATAAGCCGCAAAGCCTACGCGATTGTAACGAAAAACTGTTTACGGCTTGGTTCCCAGAGT
TAACCCCAGATACGCTGGTCAGCCGCAGTAAAGACCATATCCGCAAATTCCACCAGGAACACGGCGATATCATCCTGAAA
CCACTGGATGGCATGGGGGGTACCTCAATATTCAGAGTAAAACAAGATGACCCTAACCTCTCGGTTATCATTGAAACACT
GACCGAATTAAGCAGCCGCTTCTGCATGGCACAAAACTTCTTACCGGCCATTAAAGAAGGCGATAAACGTGTGCTAGTGG
TTGATGGCGAGCCTGTGCCTTATTGTCTGGCACGTATTCCCGCCCAAGGTGAAACCCGTGGCAATCTGGCTGCCGGTGGC
CGTGGCGAAGCCCGCCCATTGAGTGAAAGTGATTGGGAAATTGCCCGCACTGTTGCACCAATACTGAAGCAAAAAGGCTT
AATTTTTGTTGGGTTGGATATTATTGGTGATCGCTTGACTGAAATTAACGTCACCAGCCCGACCTGCGTGCGTGAAATCG
AAGCGGCCTTCCCCGAGATTTCCATTACCGGTATGTTAATGGACGCCATTGAAAGACGTTTGGCTGACAAAAAGGCCTAA

Upstream 100 bases:

>100_bases
CGAGTGCTGCGAACAGAAACTACCGCCCTTACCGCAATTACTGCGTTGCAGGTGCGGTTTGGCGATCTGGGATAACCCCA
TTTCCCACTAGGAGAAAAGA

Downstream 100 bases:

>100_bases
TACATAAAACGGGGTGTGGTGTTTTTCTAAGCGCTGCCCCCGTGACTTTATCCTCATTTATCCGCATACTGGCACCCGTT
CATCCATTAACTCAGCGACT

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MIKLGIVMDPISSINIKKDTSFAMLLEAQRRGWELHYMEMGDLYMRGGDGRARTRRLNVKQDKDNWFSFGAEQDLPLYDL
DVILMRKDPPFDTEFIYATYILERAEDKGTLVVNKPQSLRDCNEKLFTAWFPELTPDTLVSRSKDHIRKFHQEHGDIILK
PLDGMGGTSIFRVKQDDPNLSVIIETLTELSSRFCMAQNFLPAIKEGDKRVLVVDGEPVPYCLARIPAQGETRGNLAAGG
RGEARPLSESDWEIARTVAPILKQKGLIFVGLDIIGDRLTEINVTSPTCVREIEAAFPEISITGMLMDAIERRLADKKA

Sequences:

>Translated_319_residues
MIKLGIVMDPISSINIKKDTSFAMLLEAQRRGWELHYMEMGDLYMRGGDGRARTRRLNVKQDKDNWFSFGAEQDLPLYDL
DVILMRKDPPFDTEFIYATYILERAEDKGTLVVNKPQSLRDCNEKLFTAWFPELTPDTLVSRSKDHIRKFHQEHGDIILK
PLDGMGGTSIFRVKQDDPNLSVIIETLTELSSRFCMAQNFLPAIKEGDKRVLVVDGEPVPYCLARIPAQGETRGNLAAGG
RGEARPLSESDWEIARTVAPILKQKGLIFVGLDIIGDRLTEINVTSPTCVREIEAAFPEISITGMLMDAIERRLADKKA
>Mature_319_residues
MIKLGIVMDPISSINIKKDTSFAMLLEAQRRGWELHYMEMGDLYMRGGDGRARTRRLNVKQDKDNWFSFGAEQDLPLYDL
DVILMRKDPPFDTEFIYATYILERAEDKGTLVVNKPQSLRDCNEKLFTAWFPELTPDTLVSRSKDHIRKFHQEHGDIILK
PLDGMGGTSIFRVKQDDPNLSVIIETLTELSSRFCMAQNFLPAIKEGDKRVLVVDGEPVPYCLARIPAQGETRGNLAAGG
RGEARPLSESDWEIARTVAPILKQKGLIFVGLDIIGDRLTEINVTSPTCVREIEAAFPEISITGMLMDAIERRLADKKA

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1789316, Length=317, Percent_Identity=80.1261829652997, Blast_Score=531, Evalue=1e-152,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSHB_YERPE (P58582)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AG0114
- RefSeq:   NP_670620.1
- RefSeq:   NP_994784.1
- RefSeq:   YP_002345982.1
- ProteinModelPortal:   P58582
- SMR:   P58582
- IntAct:   P58582
- GeneID:   1148268
- GeneID:   1173771
- GeneID:   2764424
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO0935
- KEGG:   ypk:y3321
- KEGG:   ypm:YP_3507
- HOGENOM:   HBG624526
- OMA:   WMRKDPP
- ProtClustDB:   PRK05246
- BioCyc:   YPES187410:Y3321-MONOMER
- BioCyc:   YPES214092:YPO0935-MONOMER
- BRENDA:   6.3.2.3
- HAMAP:   MF_00162
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01380

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.3

Molecular weight: Translated: 36016; Mature: 36016

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLGIVMDPISSINIKKDTSFAMLLEAQRRGWELHYMEMGDLYMRGGDGRARTRRLNVK
CEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCEEEECCCCCCEEEEECCC
QDKDNWFSFGAEQDLPLYDLDVILMRKDPPFDTEFIYATYILERAEDKGTLVVNKPQSLR
CCCCCCEECCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEECCHHHH
DCNEKLFTAWFPELTPDTLVSRSKDHIRKFHQEHGDIILKPLDGMGGTSIFRVKQDDPNL
HHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCCCCH
SVIIETLTELSSRFCMAQNFLPAIKEGDKRVLVVDGEPVPYCLARIPAQGETRGNLAAGG
HHHHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEECCCCCCHHHHCCCCCCCCCCCCCCCC
RGEARPLSESDWEIARTVAPILKQKGLIFVGLDIIGDRLTEINVTSPTCVREIEAAFPEI
CCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEHHHHCCCCEEEECCCCHHHHHHHHHCCCE
SITGMLMDAIERRLADKKA
EEHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIKLGIVMDPISSINIKKDTSFAMLLEAQRRGWELHYMEMGDLYMRGGDGRARTRRLNVK
CEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCEEEECCCCCCEEEEECCC
QDKDNWFSFGAEQDLPLYDLDVILMRKDPPFDTEFIYATYILERAEDKGTLVVNKPQSLR
CCCCCCEECCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEECCHHHH
DCNEKLFTAWFPELTPDTLVSRSKDHIRKFHQEHGDIILKPLDGMGGTSIFRVKQDDPNL
HHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCCCCH
SVIIETLTELSSRFCMAQNFLPAIKEGDKRVLVVDGEPVPYCLARIPAQGETRGNLAAGG
HHHHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEECCCCCCHHHHCCCCCCCCCCCCCCCC
RGEARPLSESDWEIARTVAPILKQKGLIFVGLDIIGDRLTEINVTSPTCVREIEAAFPEI
CCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEHHHHCCCCEEEECCCCHHHHHHHHHCCCE
SITGMLMDAIERRLADKKA
EEHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430