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The map label for this gene is iciA

Identifier: 218928089

GI number: 218928089

Start: 1005708

End: 1006616

Strand: Direct

Name: iciA

Synonym: YPO0916

Alternate gene names: 218928089

Gene position: 1005708-1006616 (Clockwise)

Preceding gene: 218928086

Following gene: 218928100

Centisome position: 21.61

GC content: 53.91

Gene sequence:

>909_bases
ATGAAACGCCCAGACTACCGTACGCTACAAGCGCTGGACGCGGTGATCCGTGAACGCGGTTTTGAACGTGCGGCACAGAA
GCTTTGCATCACCCAATCGGCGGTATCTCAACGTATTAAACAGTTGGAGAACCTGTTTGGTCAGCCACTGCTGGTGCGCA
CGGTGCCACCACGGCCAACGGAACAAGGGCAGAAACTGCTTGCTCTACTGCACCAGGTTGAGCTGCTGGAAGAAGAGTGG
CTCGGTAATGATAACGGGGTCGATACACCGTTGCTGCTATCGCTGGCCGTGAATGCTGACAGTCTGGCGACCTGGCTATT
ACCGGCGCTGAAACCGGTGCTGGCTGATTTACCTATTCGGCTAAATTTACAAGTGGAAGATGAAACCCGGACTCAGGAAC
GTTTAAGGCGTGGTGAGGTTGTTGGCGCCGTCAGTATCCAGCCACAACCACTGCCAAGCTGTCTGGTCGACCAACTCGGT
GCTCTGGATTACCTGTTTGTGGCATCTAAGGCCTTCGCAGAACGCTATTTCCCTAATGGCGTGACCCGTTCAGCGTTGCT
GAAAGCACCCGCGGTGGCATTTGATCATCTGGATGATATGCACCAGGCGTTTTTACAGCAAAATTTCGATTTGTCACCAG
GTAGCGTGCCCTGCCATATCGTCAACTCGTCTGAAGCCTTTGTGCAGTTGGCCCGCCAAGGTACCACCTGTTGTATGATC
CCGCATCTGCAAATCGAAAAAGAGCTGGCCTCGGGCGAACTGATTGACCTGACACCGGGGTTATTGCAACGGCGGATGCT
GTTCTGGCACCGGTTTGCACCAGAAAGCCGGACCATGAGAAAGGTTACTGACGCCCTCTTATCCTACGGGCGTCAGGTAT
TACGACAGGACTCGTTTATTGGGCAGTAG

Upstream 100 bases:

>100_bases
GTGAATACCTCAGCATTGCCTTATATAAAAATGACCAAAATGTCACAGCGAAATGTGGCATAGTGTTGTATCGATTTACT
ATTTTGGAGAGATGACTCCG

Downstream 100 bases:

>100_bases
CTGTTTATGTTTACTGAGCAGCAACTGTTTTATTGGCAGTAGCTGCGATCGATGCCGGGTTGGCTCCCGTTTGCAGCTCA
AAGACCACATCAACCTGATC

Product: chromosome replication initiation inhibitor protein

Products: NA

Alternate protein names: OriC replication inhibitor

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLENLFGQPLLVRTVPPRPTEQGQKLLALLHQVELLEEEW
LGNDNGVDTPLLLSLAVNADSLATWLLPALKPVLADLPIRLNLQVEDETRTQERLRRGEVVGAVSIQPQPLPSCLVDQLG
ALDYLFVASKAFAERYFPNGVTRSALLKAPAVAFDHLDDMHQAFLQQNFDLSPGSVPCHIVNSSEAFVQLARQGTTCCMI
PHLQIEKELASGELIDLTPGLLQRRMLFWHRFAPESRTMRKVTDALLSYGRQVLRQDSFIGQ

Sequences:

>Translated_302_residues
MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLENLFGQPLLVRTVPPRPTEQGQKLLALLHQVELLEEEW
LGNDNGVDTPLLLSLAVNADSLATWLLPALKPVLADLPIRLNLQVEDETRTQERLRRGEVVGAVSIQPQPLPSCLVDQLG
ALDYLFVASKAFAERYFPNGVTRSALLKAPAVAFDHLDDMHQAFLQQNFDLSPGSVPCHIVNSSEAFVQLARQGTTCCMI
PHLQIEKELASGELIDLTPGLLQRRMLFWHRFAPESRTMRKVTDALLSYGRQVLRQDSFIGQ
>Mature_302_residues
MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLENLFGQPLLVRTVPPRPTEQGQKLLALLHQVELLEEEW
LGNDNGVDTPLLLSLAVNADSLATWLLPALKPVLADLPIRLNLQVEDETRTQERLRRGEVVGAVSIQPQPLPSCLVDQLG
ALDYLFVASKAFAERYFPNGVTRSALLKAPAVAFDHLDDMHQAFLQQNFDLSPGSVPCHIVNSSEAFVQLARQGTTCCMI
PHLQIEKELASGELIDLTPGLLQRRMLFWHRFAPESRTMRKVTDALLSYGRQVLRQDSFIGQ

Specific function: Specific inhibitor of chromosomal initiation of replication in vitro. Binds the three 13-mers in the origin (oriC) to block initiation of replication

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1789283, Length=297, Percent_Identity=91.9191919191919, Blast_Score=556, Evalue=1e-160,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ICIA_YERP3 (A7FF10)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001399838.1
- ProteinModelPortal:   A7FF10
- STRING:   A7FF10
- GeneID:   5387386
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_0854
- eggNOG:   COG0583
- HOGENOM:   HBG685425
- OMA:   YLFVASP
- ProtClustDB:   PRK03635
- BioCyc:   YPSE349747:YPSIP31758_0854-MONOMER
- HAMAP:   MF_00513
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR017685
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00039
- TIGRFAMs:   TIGR03298

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 33893; Mature: 33893

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLENLFGQPLLVRTVPPRPT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
EQGQKLLALLHQVELLEEEWLGNDNGVDTPLLLSLAVNADSLATWLLPALKPVLADLPIR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEE
LNLQVEDETRTQERLRRGEVVGAVSIQPQPLPSCLVDQLGALDYLFVASKAFAERYFPNG
EEEEECCHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHCCCC
VTRSALLKAPAVAFDHLDDMHQAFLQQNFDLSPGSVPCHIVNSSEAFVQLARQGTTCCMI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHCCCCEEEC
PHLQIEKELASGELIDLTPGLLQRRMLFWHRFAPESRTMRKVTDALLSYGRQVLRQDSFI
CCHHHHHHHHCCCEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
GQ
CC
>Mature Secondary Structure
MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLENLFGQPLLVRTVPPRPT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
EQGQKLLALLHQVELLEEEWLGNDNGVDTPLLLSLAVNADSLATWLLPALKPVLADLPIR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEE
LNLQVEDETRTQERLRRGEVVGAVSIQPQPLPSCLVDQLGALDYLFVASKAFAERYFPNG
EEEEECCHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHCCCC
VTRSALLKAPAVAFDHLDDMHQAFLQQNFDLSPGSVPCHIVNSSEAFVQLARQGTTCCMI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHCCCCEEEC
PHLQIEKELASGELIDLTPGLLQRRMLFWHRFAPESRTMRKVTDALLSYGRQVLRQDSFI
CCHHHHHHHHCCCEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
GQ
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA