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The map label for this gene is lacI [H]

Identifier: 218928025

GI number: 218928025

Start: 932764

End: 933837

Strand: Reverse

Name: lacI [H]

Synonym: YPO0849

Alternate gene names: 218928025

Gene position: 933837-932764 (Counterclockwise)

Preceding gene: 218928027

Following gene: 218928022

Centisome position: 20.07

GC content: 48.32

Gene sequence:

>1074_bases
ATGAAGTCGAAAAGCACCACATTAGAAGATGTTGCCCGCCATGCAGGTGTTTCTTATCAAACGGTTTCTCGGGTACTGAA
TAAATCGGCCAAAGTATCCGAAGCTACTCGTCGCAAGGTTGAGCAGTCAATTGAGCTGCTGCGTTATGTACCCAACCGCC
TTGCCCAACAATTGGTTGGTAAGCAAAGTATGACGATGGGTCTGGTGACCACGTCACTCGCGTTACATGCTCCATCACAG
GTCGCCGCAGCAATAAAACGCTACGCGCATATTGAAGGTTATCAAGTACTGATTTCTATGATTGATGAGAGCGTCAATCA
AAGCATTCAACATTCGATTAATGACCTAAAATCTCAGTGGGTAGGCAAGGTCATTATCAACGTCCCTCTAGAGACGGCTG
TAGCAGAGAAAATCGCTGCAGATAATGATGATGTCACTTGTTTGTTTCTCGATGTTGACCCCTATAGTTCTGTGTTTAAC
GTTTCGTTTAACCCGGCAGATGGCACCCGTGCCAGCGTCAAATATCTTTATGAATTGGGGCACCGTGAGATTGCGTTGCT
GGCAGGGCCTAAGCAGTCGGTATCGGCAAATTTGCGGCTTAAAAGCTGGCTAGAGACGCTGGCCGATTATGGTTTATCGC
CTGTCAGTGTGATCCATGGAAACTGGGATGCCCAAAGTGGGTATTCAGGCGCATTACAGATGTTGCGTGAAACACCGCAG
TTCAGTGCCGTGCTGGTGGCCAATGATCAAATGGCGCTTGGGGTATTGAGTGCGTTTCATCAAAACCAACTGGCGGTACC
GGGAGAGAAGTCGGTCATTGGTTACGATGATACTTATGAAAGTTCGTTTTTCTACCCGGCCCTCACTACCGTATCACTTG
ATTTAGATTTACAGGGAAAAGAGGCGGTTCGGCGTATGCTTAGCGCCAGTGATAACGAGTCCATGCGCTCCTCATCGATA
CTGCCTGCCAAACTGGTCGTGCGTAATTCCACGGGGCCTCAGGCTAAAGAGCACCGTGATTTACAAAAAATTGCCGAAGA
GTTGCGCCTTATTGCTCAGCGTTTGGCCAGTTGA

Upstream 100 bases:

>100_bases
ATACATTTGATGATGTGATTTATAGGTGGATTCGGGTGATTGGGTTTTTCACCTATGCCCCTGTTACAATCAAGGAATAA
CACTTAGAGGGAGCCAGACT

Downstream 100 bases:

>100_bases
ACTAGAAATAAGTTGAACCATAAACAAGTTGAACCATAAACAAGTTGAACCATAAAGTTACTGCGCTCGCCGTTTTTACC
CGTACAAAAATAAACGGGCT

Product: lac repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MKSKSTTLEDVARHAGVSYQTVSRVLNKSAKVSEATRRKVEQSIELLRYVPNRLAQQLVGKQSMTMGLVTTSLALHAPSQ
VAAAIKRYAHIEGYQVLISMIDESVNQSIQHSINDLKSQWVGKVIINVPLETAVAEKIAADNDDVTCLFLDVDPYSSVFN
VSFNPADGTRASVKYLYELGHREIALLAGPKQSVSANLRLKSWLETLADYGLSPVSVIHGNWDAQSGYSGALQMLRETPQ
FSAVLVANDQMALGVLSAFHQNQLAVPGEKSVIGYDDTYESSFFYPALTTVSLDLDLQGKEAVRRMLSASDNESMRSSSI
LPAKLVVRNSTGPQAKEHRDLQKIAEELRLIAQRLAS

Sequences:

>Translated_357_residues
MKSKSTTLEDVARHAGVSYQTVSRVLNKSAKVSEATRRKVEQSIELLRYVPNRLAQQLVGKQSMTMGLVTTSLALHAPSQ
VAAAIKRYAHIEGYQVLISMIDESVNQSIQHSINDLKSQWVGKVIINVPLETAVAEKIAADNDDVTCLFLDVDPYSSVFN
VSFNPADGTRASVKYLYELGHREIALLAGPKQSVSANLRLKSWLETLADYGLSPVSVIHGNWDAQSGYSGALQMLRETPQ
FSAVLVANDQMALGVLSAFHQNQLAVPGEKSVIGYDDTYESSFFYPALTTVSLDLDLQGKEAVRRMLSASDNESMRSSSI
LPAKLVVRNSTGPQAKEHRDLQKIAEELRLIAQRLAS
>Mature_357_residues
MKSKSTTLEDVARHAGVSYQTVSRVLNKSAKVSEATRRKVEQSIELLRYVPNRLAQQLVGKQSMTMGLVTTSLALHAPSQ
VAAAIKRYAHIEGYQVLISMIDESVNQSIQHSINDLKSQWVGKVIINVPLETAVAEKIAADNDDVTCLFLDVDPYSSVFN
VSFNPADGTRASVKYLYELGHREIALLAGPKQSVSANLRLKSWLETLADYGLSPVSVIHGNWDAQSGYSGALQMLRETPQ
FSAVLVANDQMALGVLSAFHQNQLAVPGEKSVIGYDDTYESSFFYPALTTVSLDLDLQGKEAVRRMLSASDNESMRSSSI
LPAKLVVRNSTGPQAKEHRDLQKIAEELRLIAQRLAS

Specific function: Repressor of the lactose operon. Binds lactose as an inducer [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786540, Length=328, Percent_Identity=46.0365853658537, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI1789068, Length=335, Percent_Identity=31.9402985074627, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI1790194, Length=300, Percent_Identity=31.3333333333333, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1790369, Length=342, Percent_Identity=28.6549707602339, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1787948, Length=342, Percent_Identity=27.7777777777778, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1789202, Length=349, Percent_Identity=29.512893982808, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1788474, Length=337, Percent_Identity=29.673590504451, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1787580, Length=316, Percent_Identity=25.9493670886076, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI48994940, Length=329, Percent_Identity=25.2279635258359, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1789456, Length=337, Percent_Identity=26.7062314540059, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790715, Length=325, Percent_Identity=24.3076923076923, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1790689, Length=320, Percent_Identity=25, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1787906, Length=346, Percent_Identity=26.878612716763, Blast_Score=81, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 39150; Mature: 39150

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSKSTTLEDVARHAGVSYQTVSRVLNKSAKVSEATRRKVEQSIELLRYVPNRLAQQLVG
CCCCCCHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KQSMTMGLVTTSLALHAPSQVAAAIKRYAHIEGYQVLISMIDESVNQSIQHSINDLKSQW
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGKVIINVPLETAVAEKIAADNDDVTCLFLDVDPYSSVFNVSFNPADGTRASVKYLYELG
HHHEEEECCHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHCC
HREIALLAGPKQSVSANLRLKSWLETLADYGLSPVSVIHGNWDAQSGYSGALQMLRETPQ
CCEEEEEECCCHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCCC
FSAVLVANDQMALGVLSAFHQNQLAVPGEKSVIGYDDTYESSFFYPALTTVSLDLDLQGK
CEEEEEECCHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCEEECCCEEEEEEECCCCH
EAVRRMLSASDNESMRSSSILPAKLVVRNSTGPQAKEHRDLQKIAEELRLIAQRLAS
HHHHHHHCCCCCCHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKSKSTTLEDVARHAGVSYQTVSRVLNKSAKVSEATRRKVEQSIELLRYVPNRLAQQLVG
CCCCCCHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KQSMTMGLVTTSLALHAPSQVAAAIKRYAHIEGYQVLISMIDESVNQSIQHSINDLKSQW
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGKVIINVPLETAVAEKIAADNDDVTCLFLDVDPYSSVFNVSFNPADGTRASVKYLYELG
HHHEEEECCHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHCC
HREIALLAGPKQSVSANLRLKSWLETLADYGLSPVSVIHGNWDAQSGYSGALQMLRETPQ
CCEEEEEECCCHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCCC
FSAVLVANDQMALGVLSAFHQNQLAVPGEKSVIGYDDTYESSFFYPALTTVSLDLDLQGK
CEEEEEECCHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCEEECCCEEEEEEECCCCH
EAVRRMLSASDNESMRSSSILPAKLVVRNSTGPQAKEHRDLQKIAEELRLIAQRLAS
HHHHHHHCCCCCCHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3897196 [H]