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The map label for this gene is thyA [H]

Identifier: 218927963

GI number: 218927963

Start: 859517

End: 860311

Strand: Reverse

Name: thyA [H]

Synonym: YPO0783

Alternate gene names: 218927963

Gene position: 860311-859517 (Counterclockwise)

Preceding gene: 218927964

Following gene: 218927962

Centisome position: 18.49

GC content: 46.67

Gene sequence:

>795_bases
ATGAAACAGTATCTGGATTTGATGAAAAAAGTGCTGGAAGAAGGCACACCTAAAGCTGACCGTACCGGTACCGGCACTCT
GTCAATTTTTGGGCATCAGATGCGTTTCAATTTACAAGACGGTTTCCCATTGGTCACCACCAAGCGTTGCCACCTACGTT
CGATTATCCATGAGTTGTTATGGTTCCTGAATGGCGATACCAACATTGCTTATCTGAAGGAAAATAATGTCTCAATCTGG
GATGAATGGGCAGATGAAAACGGCGATCTTGGTCCGATTTATGGTAAACAATGGCGTGCTTGGGGGGCTGCTGATGGCCG
AAAAATTGACCAGTTGAGTAACGTAGTCAATCAACTGAAGCAGGACCCTGATTCGCGCCGGATTATTGTTTCTGCGTGGA
ATGTGGGCGAACTGGATCAAATGGCGTTGGCACCATGCCATGCCTTTTTCCAGTTCTACGTGGCTGATGGCAAACTTTCT
TGCCAACTGTACCAACGCTCTTGCGATGTGTTCCTTGGCTTGCCATTTAACATTGCCAGCTATGCGTTATTAGTTCACAT
GATGGCGCAGCAGTGTGATCTGGCCGTAGGCGATTTTGTCTGGACTGGCGGGGATACTCACTTGTATAGCAACCATATTG
ACCAGGCGCATCTGCAATTGAGCCGTGAGCCACGAGTATTACCGAAACTGGTGATCAAACGTAAACCTGATTCATTGTTT
GACTACCATTTCGATGATTTTGATATTGAGGGCTACGATCCTCACCCTGGTATTAAAGCCCCTATCGCGATTTAA

Upstream 100 bases:

>100_bases
CAGCATGGGGCAGATATTGTCTGTGCCTATGATTCTGGCCGGTATTATTATGATGATTTGGGCATATCGCCGTCCTACGC
AAAAACTTTCGTGAGGTAAC

Downstream 100 bases:

>100_bases
TCTTCATATCGGAGCTGTAATGGCTCCGTTTTGCGTCTTTTATCTCTTTACCTCTTTACCTCTTTTACGCTTTTTTACCC
CTATCTTGTCTATTTTTTCC

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKQYLDLMKKVLEEGTPKADRTGTGTLSIFGHQMRFNLQDGFPLVTTKRCHLRSIIHELLWFLNGDTNIAYLKENNVSIW
DEWADENGDLGPIYGKQWRAWGAADGRKIDQLSNVVNQLKQDPDSRRIIVSAWNVGELDQMALAPCHAFFQFYVADGKLS
CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLAVGDFVWTGGDTHLYSNHIDQAHLQLSREPRVLPKLVIKRKPDSLF
DYHFDDFDIEGYDPHPGIKAPIAI

Sequences:

>Translated_264_residues
MKQYLDLMKKVLEEGTPKADRTGTGTLSIFGHQMRFNLQDGFPLVTTKRCHLRSIIHELLWFLNGDTNIAYLKENNVSIW
DEWADENGDLGPIYGKQWRAWGAADGRKIDQLSNVVNQLKQDPDSRRIIVSAWNVGELDQMALAPCHAFFQFYVADGKLS
CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLAVGDFVWTGGDTHLYSNHIDQAHLQLSREPRVLPKLVIKRKPDSLF
DYHFDDFDIEGYDPHPGIKAPIAI
>Mature_264_residues
MKQYLDLMKKVLEEGTPKADRTGTGTLSIFGHQMRFNLQDGFPLVTTKRCHLRSIIHELLWFLNGDTNIAYLKENNVSIW
DEWADENGDLGPIYGKQWRAWGAADGRKIDQLSNVVNQLKQDPDSRRIIVSAWNVGELDQMALAPCHAFFQFYVADGKLS
CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLAVGDFVWTGGDTHLYSNHIDQAHLQLSREPRVLPKLVIKRKPDSLF
DYHFDDFDIEGYDPHPGIKAPIAI

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=282, Percent_Identity=50.354609929078, Blast_Score=281, Evalue=5e-76,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=88.6363636363636, Blast_Score=503, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI71993377, Length=283, Percent_Identity=47.7031802120141, Blast_Score=269, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6324648, Length=292, Percent_Identity=40.7534246575342, Blast_Score=205, Evalue=6e-54,
Organism=Drosophila melanogaster, GI17137556, Length=283, Percent_Identity=49.1166077738516, Blast_Score=279, Evalue=1e-75,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 30112; Mature: 30112

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYLDLMKKVLEEGTPKADRTGTGTLSIFGHQMRFNLQDGFPLVTTKRCHLRSIIHELL
CHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEECCCCCCEEECCHHHHHHHHHHHH
WFLNGDTNIAYLKENNVSIWDEWADENGDLGPIYGKQWRAWGAADGRKIDQLSNVVNQLK
HHCCCCCEEEEEECCCCEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
QDPDSRRIIVSAWNVGELDQMALAPCHAFFQFYVADGKLSCQLYQRSCDVFLGLPFNIAS
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEHEECCCCCEEEECCHHHHH
YALLVHMMAQQCDLAVGDFVWTGGDTHLYSNHIDQAHLQLSREPRVLPKLVIKRKPDSLF
HHHHHHHHHHHCCCEECCEEEECCCCEEHHCCCCHHHHHHCCCCCHHHHHHHHCCCCHHH
DYHFDDFDIEGYDPHPGIKAPIAI
CCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKQYLDLMKKVLEEGTPKADRTGTGTLSIFGHQMRFNLQDGFPLVTTKRCHLRSIIHELL
CHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEECCCCCCEEECCHHHHHHHHHHHH
WFLNGDTNIAYLKENNVSIWDEWADENGDLGPIYGKQWRAWGAADGRKIDQLSNVVNQLK
HHCCCCCEEEEEECCCCEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
QDPDSRRIIVSAWNVGELDQMALAPCHAFFQFYVADGKLSCQLYQRSCDVFLGLPFNIAS
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEHEECCCCCEEEECCHHHHH
YALLVHMMAQQCDLAVGDFVWTGGDTHLYSNHIDQAHLQLSREPRVLPKLVIKRKPDSLF
HHHHHHHHHHHCCCEECCEEEECCCCEEHHCCCCHHHHHHCCCCCHHHHHHHHCCCCHHH
DYHFDDFDIEGYDPHPGIKAPIAI
CCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA