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The map label for this gene is fliA [H]

Identifier: 218927931

GI number: 218927931

Start: 803710

End: 804405

Strand: Direct

Name: fliA [H]

Synonym: YPO0745

Alternate gene names: 218927931

Gene position: 803710-804405 (Clockwise)

Preceding gene: 218927930

Following gene: 218927932

Centisome position: 17.27

GC content: 43.97

Gene sequence:

>696_bases
ATGGAAAATAGAGAGTTTGAAGCGTGCTTAACGCTCACCGCCGAAGAGGAAGGAAAATATCTGAATGCGTATTTACCGCT
GGTAAAACGTATTGTCAGGCAGCTCTCTTTCCAGGCGGATAGCGTCATCGGCAAGGAGGATATGCAGCAAATTGCATTGA
TGGGGTTATTAGAGGCACTGCGCCGCTATGGTCACCCTGACGGGCAATTTGCCGCTTATGCCGTTCATCGGATCCGAGGT
TCAATATTGGATCAATTGAGGGAGCATGATTGGCGACCCCGCCGGTTGCGGCAGAAAACCCATAAAACCAATGAGGCTAT
CCGGCAAATCGCGAAACGTCTGGGCCATGAACCTAACTTTGAGGAGATCAGCGCTGAATTAAACCTGACGGCAGATGAAT
ATCAGGAATATCTTTTGTTGGAGAATGCGGGTGCGATGGAAAGCCTGGATGAAATTCTCGCATTAGAGGCTCACAATGAT
ATTTTGCAAAGTCGGGATCTGGAAGAGAATATTATTATTGAAGATAATCTCAAACAGGCGATTAGCACATTAGATGAACG
TGAACAGATGATTTTGCATCTTTATTATCAGCAAGAACTGAGTATGAAAGAGATTGCACAAGTACTGGATCTCACGGAGG
CACGCATCTGCCAACTTAATAAGAAATTAGTACAAAAAATTAAATCATTTTTTTAA

Upstream 100 bases:

>100_bases
GCCAGCTAATGAATGAATATAAGCAAGATTTCGCAGGATTAAATGTGCCGATGCCATTTGATGACGTGGTTATCAGCAGA
ATGGTCTTTCAATAATCATA

Downstream 100 bases:

>100_bases
ATTATCGAGGGTGTTATGCAGAAGCTGTTTGGCCTACTGGTCATTATGGGGTGTGTGATTGGTGGCTACTTAATGTCTGG
CGGTTCGCTATCGTCATTTT

Product: flagellar biosynthesis sigma factor

Products: NA

Alternate protein names: Sigma-27; Sigma-F factor [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MENREFEACLTLTAEEEGKYLNAYLPLVKRIVRQLSFQADSVIGKEDMQQIALMGLLEALRRYGHPDGQFAAYAVHRIRG
SILDQLREHDWRPRRLRQKTHKTNEAIRQIAKRLGHEPNFEEISAELNLTADEYQEYLLLENAGAMESLDEILALEAHND
ILQSRDLEENIIIEDNLKQAISTLDEREQMILHLYYQQELSMKEIAQVLDLTEARICQLNKKLVQKIKSFF

Sequences:

>Translated_231_residues
MENREFEACLTLTAEEEGKYLNAYLPLVKRIVRQLSFQADSVIGKEDMQQIALMGLLEALRRYGHPDGQFAAYAVHRIRG
SILDQLREHDWRPRRLRQKTHKTNEAIRQIAKRLGHEPNFEEISAELNLTADEYQEYLLLENAGAMESLDEILALEAHND
ILQSRDLEENIIIEDNLKQAISTLDEREQMILHLYYQQELSMKEIAQVLDLTEARICQLNKKLVQKIKSFF
>Mature_231_residues
MENREFEACLTLTAEEEGKYLNAYLPLVKRIVRQLSFQADSVIGKEDMQQIALMGLLEALRRYGHPDGQFAAYAVHRIRG
SILDQLREHDWRPRRLRQKTHKTNEAIRQIAKRLGHEPNFEEISAELNLTADEYQEYLLLENAGAMESLDEILALEAHND
ILQSRDLEENIIIEDNLKQAISTLDEREQMILHLYYQQELSMKEIAQVLDLTEARICQLNKKLVQKIKSFF

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellin gene (fliC) expression [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=217, Percent_Identity=34.5622119815668, Blast_Score=124, Evalue=7e-30,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 26979; Mature: 26979

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENREFEACLTLTAEEEGKYLNAYLPLVKRIVRQLSFQADSVIGKEDMQQIALMGLLEAL
CCCCHHHHHHEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RRYGHPDGQFAAYAVHRIRGSILDQLREHDWRPRRLRQKTHKTNEAIRQIAKRLGHEPNF
HHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCH
EEISAELNLTADEYQEYLLLENAGAMESLDEILALEAHNDILQSRDLEENIIIEDNLKQA
HHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHH
ISTLDEREQMILHLYYQQELSMKEIAQVLDLTEARICQLNKKLVQKIKSFF
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MENREFEACLTLTAEEEGKYLNAYLPLVKRIVRQLSFQADSVIGKEDMQQIALMGLLEAL
CCCCHHHHHHEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RRYGHPDGQFAAYAVHRIRGSILDQLREHDWRPRRLRQKTHKTNEAIRQIAKRLGHEPNF
HHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCH
EEISAELNLTADEYQEYLLLENAGAMESLDEILALEAHNDILQSRDLEENIIIEDNLKQA
HHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHH
ISTLDEREQMILHLYYQQELSMKEIAQVLDLTEARICQLNKKLVQKIKSFF
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501040; 12620739 [H]