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The map label for this gene is fliF [H]

Identifier: 218927901

GI number: 218927901

Start: 775094

End: 776734

Strand: Direct

Name: fliF [H]

Synonym: YPO0714

Alternate gene names: 218927901

Gene position: 775094-776734 (Clockwise)

Preceding gene: 218927900

Following gene: 218927902

Centisome position: 16.66

GC content: 50.21

Gene sequence:

>1641_bases
GTGTTAGATAAAATTAAGCAGAAATTGTCACCTGGTCAATTAGGTGAAAATACAAAAACCATTCTTATGAGCGCGGTAGC
CCTTTTGGTTACCGCCGCGATTATTTTCAGTCTCTGGCGTAGCTCACAGGGCTACACGGCATTGTTTGGCTCGCAGGAGA
ATATCCCGATCACCCAAGTGGTGGAAGTGCTGGAAGGAGAGGCCATTGCCTATCGCATAAACCCGGATAATGGTCAGGTG
TTAGTGGCTGAAAATCAGTTGGGTAAAGCGCGTATCTTGCTGGCAGCAAAAGGAATTACTGCCACTCTCCCCATTGGCTA
TGAATTGATGGATAAAGAAAGCATGCTTGGCAGCAGCCAGTTTATCCAAAATGTTCGTTATAAGCGCAGCCTGGAAGGGG
AACTGGCGCAGAGCATGATGGCATTGAGCGCGGTGGAATATGCCAGAGTCCATCTGGGCATGAGTGAAGCCAGTTCATTC
GCCATCAGTAATCATGCAGATAACAGTGCCTCGGTGGTGTTACGGCTGAGATACGGCCAAACATTGAGCACTGAACAGGT
GGGGGCCATTGTCCAATTGGTGGCGGGCAGTATTCCCGGAATGAAACCTGCCAATGTGCGGGTGGTCGATCAACACGGTG
AATTATTGTCACAAGCTTATCAAGCGAACAGTGAGGGGGTGCCCAGTGTGAAAAGTGGGACCGAATTAGCTCACTATTTG
CAAAGCACCACTGAGAAGAATATCGCGAACCTGTTGAATTCGGTGATTGGCGCGAATAACTACCGCATCAGTGTCTCTAC
CCAGTTGGATATGAGCCGTATTGAAGAGACGGCGGAGCACTATGGCCCGGACCCTCGAATCAATGATGAAAATATCCAGC
AGGAGAACAGCAACGACGATATGGCGATGGGGATCCCCGGATCACTAAGCAATCAGCCCATCCCTCAATCTCAGGCGGGT
CAAACGCCAGCCGCCGTCAGCCGCAGCCAGGCGCAGCGTAAATATATTTATGATCGCAATATCCGCCATGTGCGTTATCC
GGGTTACAAGCTGGAGAAAATGACCGTTGCGGTGGTGCTGAATAAATCGCTCCCGGTGTTGGAACAGTGGACGCCAGAAC
AGCAGGAAGAACTCAAACGTTTAATTGAAGATGCCGCAGGTATTGATGTTAAACGTGGGGATTCTCTGACCATTAACATG
ATGGCCTTCGCGGTCCCTACGTTGATTGACGAGCCGGTGATGCCGTGGTGGCAAGAGCCAAGTACCTTCCGTTGGGCAGA
ATTGCTGGGGATCGGGCTGCTGTCATTACTGGTGCTGTGGTTTGGTGTTCGCCCATTGATGAAACGCTATTCGCGTAAGG
GTTCGGAGAACCTGCCGTTAGCCATTTCCTCGGCGAGTGCAGATGAGGCGCTTGATCATGTGGATACCGGTGTAGACGGT
GCAGAAAGTAGCCCGCGTACAGAAAATGCCTTCTCCGCATCTTCTCTTTGGAAAAGCGACGATCTACCAGAGCAAGGCTC
CGGTTTAGAAACCAAAATCGCCCATCTGCAACAATTGGCGCAGTCAGAAACTGAACGTACCGCCGAAGTCATTAAACAAT
GGATTAACAGTAATGAACGAATCAAATCTCAACCTGAATGA

Upstream 100 bases:

>100_bases
CCAGAAAGCCAGTGTGGCTTTCTCCGCCATGGTGCAGGTGCGCAATAAGCTCACTTCTGCATTAGATGATGTTATGAATA
CTGCGCTGTAGGGTAAATCG

Downstream 100 bases:

>100_bases
TGACTCAAGCGTCAAAAGTGATACCAAGGGGCGCAGCCGATTGGAACAGGCCAGTATTTTATTACTGAGTATTGGTGAAG
AAGCGGCCGCCATGGTGATG

Product: flagellar MS-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MLDKIKQKLSPGQLGENTKTILMSAVALLVTAAIIFSLWRSSQGYTALFGSQENIPITQVVEVLEGEAIAYRINPDNGQV
LVAENQLGKARILLAAKGITATLPIGYELMDKESMLGSSQFIQNVRYKRSLEGELAQSMMALSAVEYARVHLGMSEASSF
AISNHADNSASVVLRLRYGQTLSTEQVGAIVQLVAGSIPGMKPANVRVVDQHGELLSQAYQANSEGVPSVKSGTELAHYL
QSTTEKNIANLLNSVIGANNYRISVSTQLDMSRIEETAEHYGPDPRINDENIQQENSNDDMAMGIPGSLSNQPIPQSQAG
QTPAAVSRSQAQRKYIYDRNIRHVRYPGYKLEKMTVAVVLNKSLPVLEQWTPEQQEELKRLIEDAAGIDVKRGDSLTINM
MAFAVPTLIDEPVMPWWQEPSTFRWAELLGIGLLSLLVLWFGVRPLMKRYSRKGSENLPLAISSASADEALDHVDTGVDG
AESSPRTENAFSASSLWKSDDLPEQGSGLETKIAHLQQLAQSETERTAEVIKQWINSNERIKSQPE

Sequences:

>Translated_546_residues
MLDKIKQKLSPGQLGENTKTILMSAVALLVTAAIIFSLWRSSQGYTALFGSQENIPITQVVEVLEGEAIAYRINPDNGQV
LVAENQLGKARILLAAKGITATLPIGYELMDKESMLGSSQFIQNVRYKRSLEGELAQSMMALSAVEYARVHLGMSEASSF
AISNHADNSASVVLRLRYGQTLSTEQVGAIVQLVAGSIPGMKPANVRVVDQHGELLSQAYQANSEGVPSVKSGTELAHYL
QSTTEKNIANLLNSVIGANNYRISVSTQLDMSRIEETAEHYGPDPRINDENIQQENSNDDMAMGIPGSLSNQPIPQSQAG
QTPAAVSRSQAQRKYIYDRNIRHVRYPGYKLEKMTVAVVLNKSLPVLEQWTPEQQEELKRLIEDAAGIDVKRGDSLTINM
MAFAVPTLIDEPVMPWWQEPSTFRWAELLGIGLLSLLVLWFGVRPLMKRYSRKGSENLPLAISSASADEALDHVDTGVDG
AESSPRTENAFSASSLWKSDDLPEQGSGLETKIAHLQQLAQSETERTAEVIKQWINSNERIKSQPE
>Mature_546_residues
MLDKIKQKLSPGQLGENTKTILMSAVALLVTAAIIFSLWRSSQGYTALFGSQENIPITQVVEVLEGEAIAYRINPDNGQV
LVAENQLGKARILLAAKGITATLPIGYELMDKESMLGSSQFIQNVRYKRSLEGELAQSMMALSAVEYARVHLGMSEASSF
AISNHADNSASVVLRLRYGQTLSTEQVGAIVQLVAGSIPGMKPANVRVVDQHGELLSQAYQANSEGVPSVKSGTELAHYL
QSTTEKNIANLLNSVIGANNYRISVSTQLDMSRIEETAEHYGPDPRINDENIQQENSNDDMAMGIPGSLSNQPIPQSQAG
QTPAAVSRSQAQRKYIYDRNIRHVRYPGYKLEKMTVAVVLNKSLPVLEQWTPEQQEELKRLIEDAAGIDVKRGDSLTINM
MAFAVPTLIDEPVMPWWQEPSTFRWAELLGIGLLSLLVLWFGVRPLMKRYSRKGSENLPLAISSASADEALDHVDTGVDG
AESSPRTENAFSASSLWKSDDLPEQGSGLETKIAHLQQLAQSETERTAEVIKQWINSNERIKSQPE

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=569, Percent_Identity=29.1739894551845, Blast_Score=182, Evalue=7e-47,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 59947; Mature: 59947

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDKIKQKLSPGQLGENTKTILMSAVALLVTAAIIFSLWRSSQGYTALFGSQENIPITQV
CCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHH
VEVLEGEAIAYRINPDNGQVLVAENQLGKARILLAAKGITATLPIGYELMDKESMLGSSQ
HHHHCCCEEEEEECCCCCEEEEEECCCCCEEEEEEECCCEEECCCCHHHHHHHHHCCHHH
FIQNVRYKRSLEGELAQSMMALSAVEYARVHLGMSEASSFAISNHADNSASVVLRLRYGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEEECCCCCCCEEEEEEEECCC
TLSTEQVGAIVQLVAGSIPGMKPANVRVVDQHGELLSQAYQANSEGVPSVKSGTELAHYL
CCCHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
QSTTEKNIANLLNSVIGANNYRISVSTQLDMSRIEETAEHYGPDPRINDENIQQENSNDD
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCHHCCCCCCCCC
MAMGIPGSLSNQPIPQSQAGQTPAAVSRSQAQRKYIYDRNIRHVRYPGYKLEKMTVAVVL
EEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCEEEHHEEEEEEE
NKSLPVLEQWTPEQQEELKRLIEDAAGIDVKRGDSLTINMMAFAVPTLIDEPVMPWWQEP
CCCCCHHHHCCCHHHHHHHHHHHHHCCCEEECCCCEEEEHHHHHHHHHHCCCCCCCCCCC
STFRWAELLGIGLLSLLVLWFGVRPLMKRYSRKGSENLPLAISSASADEALDHVDTGVDG
CCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHCCCCC
AESSPRTENAFSASSLWKSDDLPEQGSGLETKIAHLQQLAQSETERTAEVIKQWINSNER
CCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IKSQPE
CCCCCC
>Mature Secondary Structure
MLDKIKQKLSPGQLGENTKTILMSAVALLVTAAIIFSLWRSSQGYTALFGSQENIPITQV
CCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHH
VEVLEGEAIAYRINPDNGQVLVAENQLGKARILLAAKGITATLPIGYELMDKESMLGSSQ
HHHHCCCEEEEEECCCCCEEEEEECCCCCEEEEEEECCCEEECCCCHHHHHHHHHCCHHH
FIQNVRYKRSLEGELAQSMMALSAVEYARVHLGMSEASSFAISNHADNSASVVLRLRYGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEEECCCCCCCEEEEEEEECCC
TLSTEQVGAIVQLVAGSIPGMKPANVRVVDQHGELLSQAYQANSEGVPSVKSGTELAHYL
CCCHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
QSTTEKNIANLLNSVIGANNYRISVSTQLDMSRIEETAEHYGPDPRINDENIQQENSNDD
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCHHCCCCCCCCC
MAMGIPGSLSNQPIPQSQAGQTPAAVSRSQAQRKYIYDRNIRHVRYPGYKLEKMTVAVVL
EEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCEEEHHEEEEEEE
NKSLPVLEQWTPEQQEELKRLIEDAAGIDVKRGDSLTINMMAFAVPTLIDEPVMPWWQEP
CCCCCHHHHCCCHHHHHHHHHHHHHCCCEEECCCCEEEEHHHHHHHHHHCCCCCCCCCCC
STFRWAELLGIGLLSLLVLWFGVRPLMKRYSRKGSENLPLAISSASADEALDHVDTGVDG
CCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHCCCCC
AESSPRTENAFSASSLWKSDDLPEQGSGLETKIAHLQQLAQSETERTAEVIKQWINSNER
CCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IKSQPE
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8675317; 10984043 [H]