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The map label for this gene is flbD [H]

Identifier: 218927899

GI number: 218927899

Start: 773659

End: 774687

Strand: Direct

Name: flbD [H]

Synonym: YPO0712

Alternate gene names: 218927899

Gene position: 773659-774687 (Clockwise)

Preceding gene: 218927891

Following gene: 218927900

Centisome position: 16.62

GC content: 44.61

Gene sequence:

>1029_bases
ATGATGAATATCAATGTTGATGTTTTTCCAAAAAACGATCATGGATTTGTTGCTAATGCGCCTTCCAGCGTAAGTGTATT
TTCCCTTGCGCGTAGGGTAGCTGAATTTAATGTTCCGGTGTTGGTCACCGGTGAAACCGGAACCGGTAAAGAGTGTGTAG
CGAAATATATCCATCAGAAAGCCATGGGGGATGCCTCACCCTATATCGCGGTGAATTGTGCCGCGATACCTGAGAGCATG
TTGGAAGCCATATTATTTGGTTATGAGAAAGGTGCCTTTACGGGCGCTATCGCCAGTGTTGCAGGGAAATTCGAGCAGGC
GAATGGAGGCACATTATTACTGGATGAAATCGGTGATATGCCGTTGGCACTGCAAGTAAAGTTATTGCGTGTATTGCAAG
AGCAAGAAGTTGAACGTTTAGGTGGTCATAAGCCTATTCCTTTGGATATTCGTATTATTGCCTCAACAAACAAAGATCTG
AGTGTTGAAATCGCGGAAGGGCGTTTTCGTCAGGATCTCTACTACCGCTTATCCGTGGTGCCTATCCATATATTACCTTT
ACGTGAACGGCCAGAGGATATTCTGCCTTTGGTGAAGGCATTTATTAATAAGTACCAAAGTTTTCTGAATGTAAAAATAG
ATATCACCGCAGAAGCGCAATGTGAGCTGTATAAATACACCTGGCCTGGCAATGTTCGTGAATTAGAGAATGTTATTCAA
CGCGGTATCATCATGAGCAACAACGGCGTGATTGAACTGCCCAGCCTCGGTTTACCTATGGCACAGGGAATATCCTCGCC
AGTAGGGGAAACATCATTACCTTTTTCAACGATTCAGCCCCCTGATGGGGAAAACAATATTAAGTTACGTGGGCGCTTGG
CGCAGTACCAATACATTGTGGATTTACTGCAACGGCATCAGGGTAATAAATCTAAAACGGCCGCATTTTTAGGTATTACG
CCACGTGCTTTGCGATATCGGCTCGCCAATATGCGTGAAGATGGCATCGACATTGAGTGTCACTCATAA

Upstream 100 bases:

>100_bases
GTTACGATTTATTTTTTCAATGATGTTTAAGTCTCAATTTATTTTGTCGATCTATTGAGTGTCGGACGTGATTTATTGTG
ATTTGACGTGTGGATAAATT

Downstream 100 bases:

>100_bases
AAAATAAGGAAGCAGGATGGATAACATTGATTCTATCAATATGCCTCGCTCGCAATCACCAGTGATGCTGGAAATGCGGC
AAATGGCAGCCATGGCAGGC

Product: sigma-54 transcriptional regulator

Products: NA

Alternate protein names: Ornithine decarboxylase antizyme; Ornithine/arginine decarboxylase inhibitor [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MMNINVDVFPKNDHGFVANAPSSVSVFSLARRVAEFNVPVLVTGETGTGKECVAKYIHQKAMGDASPYIAVNCAAIPESM
LEAILFGYEKGAFTGAIASVAGKFEQANGGTLLLDEIGDMPLALQVKLLRVLQEQEVERLGGHKPIPLDIRIIASTNKDL
SVEIAEGRFRQDLYYRLSVVPIHILPLRERPEDILPLVKAFINKYQSFLNVKIDITAEAQCELYKYTWPGNVRELENVIQ
RGIIMSNNGVIELPSLGLPMAQGISSPVGETSLPFSTIQPPDGENNIKLRGRLAQYQYIVDLLQRHQGNKSKTAAFLGIT
PRALRYRLANMREDGIDIECHS

Sequences:

>Translated_342_residues
MMNINVDVFPKNDHGFVANAPSSVSVFSLARRVAEFNVPVLVTGETGTGKECVAKYIHQKAMGDASPYIAVNCAAIPESM
LEAILFGYEKGAFTGAIASVAGKFEQANGGTLLLDEIGDMPLALQVKLLRVLQEQEVERLGGHKPIPLDIRIIASTNKDL
SVEIAEGRFRQDLYYRLSVVPIHILPLRERPEDILPLVKAFINKYQSFLNVKIDITAEAQCELYKYTWPGNVRELENVIQ
RGIIMSNNGVIELPSLGLPMAQGISSPVGETSLPFSTIQPPDGENNIKLRGRLAQYQYIVDLLQRHQGNKSKTAAFLGIT
PRALRYRLANMREDGIDIECHS
>Mature_342_residues
MMNINVDVFPKNDHGFVANAPSSVSVFSLARRVAEFNVPVLVTGETGTGKECVAKYIHQKAMGDASPYIAVNCAAIPESM
LEAILFGYEKGAFTGAIASVAGKFEQANGGTLLLDEIGDMPLALQVKLLRVLQEQEVERLGGHKPIPLDIRIIASTNKDL
SVEIAEGRFRQDLYYRLSVVPIHILPLRERPEDILPLVKAFINKYQSFLNVKIDITAEAQCELYKYTWPGNVRELENVIQ
RGIIMSNNGVIELPSLGLPMAQGISSPVGETSLPFSTIQPPDGENNIKLRGRLAQYQYIVDLLQRHQGNKSKTAAFLGIT
PRALRYRLANMREDGIDIECHS

Specific function: Member of the two-component regulatory system AtoS/AtoC involved in the transcriptional regulation of the ato genes for acetoacetate metabolism. Also an inhibitor of polyamine biosynthesis [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=324, Percent_Identity=45.679012345679, Blast_Score=276, Evalue=1e-75,
Organism=Escherichia coli, GI87082117, Length=267, Percent_Identity=48.689138576779, Blast_Score=249, Evalue=1e-67,
Organism=Escherichia coli, GI1789087, Length=325, Percent_Identity=45.5384615384615, Blast_Score=245, Evalue=3e-66,
Organism=Escherichia coli, GI1790437, Length=316, Percent_Identity=40.1898734177215, Blast_Score=227, Evalue=8e-61,
Organism=Escherichia coli, GI1790299, Length=339, Percent_Identity=37.4631268436578, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI1786524, Length=222, Percent_Identity=49.5495495495495, Blast_Score=216, Evalue=1e-57,
Organism=Escherichia coli, GI1788905, Length=314, Percent_Identity=39.4904458598726, Blast_Score=214, Evalue=5e-57,
Organism=Escherichia coli, GI87082152, Length=257, Percent_Identity=44.3579766536965, Blast_Score=214, Evalue=6e-57,
Organism=Escherichia coli, GI1789233, Length=214, Percent_Identity=51.8691588785047, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1787583, Length=258, Percent_Identity=38.3720930232558, Blast_Score=187, Evalue=6e-49,
Organism=Escherichia coli, GI87081872, Length=325, Percent_Identity=36.9230769230769, Blast_Score=187, Evalue=6e-49,
Organism=Escherichia coli, GI87081858, Length=350, Percent_Identity=29.1428571428571, Blast_Score=137, Evalue=8e-34,
Organism=Escherichia coli, GI1789828, Length=225, Percent_Identity=34.2222222222222, Blast_Score=134, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 37759; Mature: 37759

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNINVDVFPKNDHGFVANAPSSVSVFSLARRVAEFNVPVLVTGETGTGKECVAKYIHQK
CCEEEEEEEECCCCCEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHH
AMGDASPYIAVNCAAIPESMLEAILFGYEKGAFTGAIASVAGKFEQANGGTLLLDEIGDM
HCCCCCCEEEEEEHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCC
PLALQVKLLRVLQEQEVERLGGHKPIPLDIRIIASTNKDLSVEIAEGRFRQDLYYRLSVV
CHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCEEEEECCCCHHHHHEEEEEEE
PIHILPLRERPEDILPLVKAFINKYQSFLNVKIDITAEAQCELYKYTWPGNVRELENVIQ
EEEEEECCCCCHHHHHHHHHHHHHHHHHEEEEEEEEECCEEEEEEEECCCCHHHHHHHHH
RGIIMSNNGVIELPSLGLPMAQGISSPVGETSLPFSTIQPPDGENNIKLRGRLAQYQYIV
CCEEECCCCEEEECCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHH
DLLQRHQGNKSKTAAFLGITPRALRYRLANMREDGIDIECHS
HHHHHHCCCCCCEEEEEECCHHHHHHHHHCCCCCCCEEEECC
>Mature Secondary Structure
MMNINVDVFPKNDHGFVANAPSSVSVFSLARRVAEFNVPVLVTGETGTGKECVAKYIHQK
CCEEEEEEEECCCCCEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHH
AMGDASPYIAVNCAAIPESMLEAILFGYEKGAFTGAIASVAGKFEQANGGTLLLDEIGDM
HCCCCCCEEEEEEHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCC
PLALQVKLLRVLQEQEVERLGGHKPIPLDIRIIASTNKDLSVEIAEGRFRQDLYYRLSVV
CHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCEEEEECCCCHHHHHEEEEEEE
PIHILPLRERPEDILPLVKAFINKYQSFLNVKIDITAEAQCELYKYTWPGNVRELENVIQ
EEEEEECCCCCHHHHHHHHHHHHHHHHHEEEEEEEEECCEEEEEEEECCCCHHHHHHHHH
RGIIMSNNGVIELPSLGLPMAQGISSPVGETSLPFSTIQPPDGENNIKLRGRLAQYQYIV
CCEEECCCCEEEECCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHH
DLLQRHQGNKSKTAAFLGITPRALRYRLANMREDGIDIECHS
HHHHHHCCCCCCEEEEEECCHHHHHHHHHCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 8346225 [H]