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The map label for this gene is rfaE

Identifier: 218927847

GI number: 218927847

Start: 714210

End: 715640

Strand: Direct

Name: rfaE

Synonym: YPO0654

Alternate gene names: 218927847

Gene position: 714210-715640 (Clockwise)

Preceding gene: 218927846

Following gene: 218927848

Centisome position: 15.35

GC content: 52.97

Gene sequence:

>1431_bases
ATGAAAGTCACGCTGCCTGATTTTCGCCGTGCTGGTGTATTAGTCGTGGGTGATGTCATGTTAGACCGCTACTGGTACGG
GCCAACCTGCCGGATTTCACCAGAAGCACCTGTGCCTGTAGTGAAAGTCGATACCATCGAAGAGCGCCCTGGTGGTGCAG
CAAACGTTGCGATGAATATTGCCTCCCTTGGCGCGGTTGCGCGCTTAGTCGGGTTGACCGGCATTGACGATGCGGCGCGT
GCGCTGATCTGCAAGCTGAGCGAAGTTCGGGTGCGTTGCGATTTTGTCTCGGTACCCACTCACCCGACCATTACCAAATT
ACGGGTACTTTCCCGTAACCAGCAGTTAATCCGCCTGGATTTTGAAGAGGGCTTTGACGGTGTTGATCCCACGCCGATAT
TCGAGCGCATTCAACTGGCGTTGCCACAGATTGGCGCGCTGGTGCTGTCCGATTATGCCAAGGGGGCATTGAACAGCGTA
CAGCCGATGATCCAACTGGCACGTAAAGCGAATGTCCCGGTTTTGATTGATCCGAAAGGCAGTGATTTTGAGCGCTACCG
TGGTGCCACATTGTTGACGCCGAATTTGTCTGAATTTGAAGCAGTGGTAGGGCGCTGTAAAAATGAAGAAGAGTTGGTTA
ACCGCGGTATGCAACTGGTGGCAGATTTCGAACTTTCAGCATTGCTGGTGACCCGTTCTGAACAAGGCATGACCTTGTTG
CAACTGGGTAAACCGCCGCTACATTTGCCGACTCAGGCAAAAGAAGTGTTTGATGTGACCGGTGCTGGCGATACGGTTAT
TGGCGTGCTGGCTGCCGCGCTGGCTGCAGGTAACAGCCTGGAAGAGTCATGTTTCCTGGCTAATGCTGCCGCCGGTGTAG
TGGTGGGTAAATTGGGTACCTCGACCGTTTCTCCGATTGAGTTGGAAAACGCGATCCGTGGCCGTGCAGAAACCGGTTTT
GGGGTGATGGATGAGCAGCAACTGAAAATAGCGGTTGCGCAGGCTCGCCAGCGTGGGGAGAAGGTGGTCATGACCAATGG
CATTTTTGACATCCTCCATGCAGGCCATGTTTCTTATCTGGCCAATGCCCGTAAGCTGGGGGATCGCCTGATTGTGGCGG
TGAACAGCGATGCTTCCACCAAGCGGCTGAAAGGTGAAAAGCGGCCGGTTAATCCATTAGAACAACGTATGGTTGTCTTG
GGTGCGTTGGAAGCGGTGGATTGGGTCGTGCCCTTTGAAGAAGATACGCCACAGCGTTTAATTGCTGATATTTTGCCCGA
TCTGTTAGTCAAAGGCGGCGATTATAAGCCCCATGAAATTGCAGGTAGCGAAGAAGTCTGGGCCGCCGGTGGCGAGGTTA
AGGTACTGAATTTTGAAGATGGTGTCTCTACCACCAATATTATTCAGTCGATCAAAAACGGCCGCGGCTAA

Upstream 100 bases:

>100_bases
TGCCTGTGATATTATTTGGCGCTATATGATGAACACAATATTGGAGCGAAGGGATGGGTGCATTCGGGGTTAATAACAAC
TCAGGTTGGGGGGCAAATGT

Downstream 100 bases:

>100_bases
GTCAGCGCCAGAGCGCTGGCGCATAAGGATGTGGCAGTGGGTGGGTTAAAGCAAGAGTTAAGTCTGGCGCAAGGCGTTGG
GTTGTTATCCACTTCCCTGT

Product: bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MKVTLPDFRRAGVLVVGDVMLDRYWYGPTCRISPEAPVPVVKVDTIEERPGGAANVAMNIASLGAVARLVGLTGIDDAAR
ALICKLSEVRVRCDFVSVPTHPTITKLRVLSRNQQLIRLDFEEGFDGVDPTPIFERIQLALPQIGALVLSDYAKGALNSV
QPMIQLARKANVPVLIDPKGSDFERYRGATLLTPNLSEFEAVVGRCKNEEELVNRGMQLVADFELSALLVTRSEQGMTLL
QLGKPPLHLPTQAKEVFDVTGAGDTVIGVLAAALAAGNSLEESCFLANAAAGVVVGKLGTSTVSPIELENAIRGRAETGF
GVMDEQQLKIAVAQARQRGEKVVMTNGIFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGEKRPVNPLEQRMVVL
GALEAVDWVVPFEEDTPQRLIADILPDLLVKGGDYKPHEIAGSEEVWAAGGEVKVLNFEDGVSTTNIIQSIKNGRG

Sequences:

>Translated_476_residues
MKVTLPDFRRAGVLVVGDVMLDRYWYGPTCRISPEAPVPVVKVDTIEERPGGAANVAMNIASLGAVARLVGLTGIDDAAR
ALICKLSEVRVRCDFVSVPTHPTITKLRVLSRNQQLIRLDFEEGFDGVDPTPIFERIQLALPQIGALVLSDYAKGALNSV
QPMIQLARKANVPVLIDPKGSDFERYRGATLLTPNLSEFEAVVGRCKNEEELVNRGMQLVADFELSALLVTRSEQGMTLL
QLGKPPLHLPTQAKEVFDVTGAGDTVIGVLAAALAAGNSLEESCFLANAAAGVVVGKLGTSTVSPIELENAIRGRAETGF
GVMDEQQLKIAVAQARQRGEKVVMTNGIFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGEKRPVNPLEQRMVVL
GALEAVDWVVPFEEDTPQRLIADILPDLLVKGGDYKPHEIAGSEEVWAAGGEVKVLNFEDGVSTTNIIQSIKNGRG
>Mature_476_residues
MKVTLPDFRRAGVLVVGDVMLDRYWYGPTCRISPEAPVPVVKVDTIEERPGGAANVAMNIASLGAVARLVGLTGIDDAAR
ALICKLSEVRVRCDFVSVPTHPTITKLRVLSRNQQLIRLDFEEGFDGVDPTPIFERIQLALPQIGALVLSDYAKGALNSV
QPMIQLARKANVPVLIDPKGSDFERYRGATLLTPNLSEFEAVVGRCKNEEELVNRGMQLVADFELSALLVTRSEQGMTLL
QLGKPPLHLPTQAKEVFDVTGAGDTVIGVLAAALAAGNSLEESCFLANAAAGVVVGKLGTSTVSPIELENAIRGRAETGF
GVMDEQQLKIAVAQARQRGEKVVMTNGIFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGEKRPVNPLEQRMVVL
GALEAVDWVVPFEEDTPQRLIADILPDLLVKGGDYKPHEIAGSEEVWAAGGEVKVLNFEDGVSTTNIIQSIKNGRG

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family

Homologues:

Organism=Escherichia coli, GI1789432, Length=475, Percent_Identity=86.1052631578947, Blast_Score=835, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HLDE_YERPA (Q1C374)

Other databases:

- EMBL:   CP000308
- RefSeq:   YP_653043.1
- ProteinModelPortal:   Q1C374
- SMR:   Q1C374
- STRING:   Q1C374
- GeneID:   4118596
- GenomeReviews:   CP000308_GR
- KEGG:   ypa:YPA_3136
- eggNOG:   COG2870
- HOGENOM:   HBG545535
- OMA:   LTPNRKE
- ProtClustDB:   PRK11316
- BioCyc:   YPES360102:YPA_3136-MONOMER
- HAMAP:   MF_01603
- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00125
- TIGRFAMs:   TIGR02198
- TIGRFAMs:   TIGR02199

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB

EC number: 2.7.-.-

Molecular weight: Translated: 51219; Mature: 51219

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00583 PFKB_KINASES_1; PS00584 PFKB_KINASES_2

Important sites: ACT_SITE 264-264

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVTLPDFRRAGVLVVGDVMLDRYWYGPTCRISPEAPVPVVKVDTIEERPGGAANVAMNI
CCCCCCCHHHCCEEEEEHHHHHHEECCCCEEECCCCCCCEEEEECCCCCCCCHHHHHHHH
ASLGAVARLVGLTGIDDAARALICKLSEVRVRCDFVSVPTHPTITKLRVLSRNQQLIRLD
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHCCCCEEEEEE
FEEGFDGVDPTPIFERIQLALPQIGALVLSDYAKGALNSVQPMIQLARKANVPVLIDPKG
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
SDFERYRGATLLTPNLSEFEAVVGRCKNEEELVNRGMQLVADFELSALLVTRSEQGMTLL
CCHHHHCCCEEECCCHHHHHHHHHCCCCHHHHHHCCHHEEECCCEEEEEEEECCCCCEEE
QLGKPPLHLPTQAKEVFDVTGAGDTVIGVLAAALAAGNSLEESCFLANAAAGVVVGKLGT
ECCCCCCCCCCCHHHHEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEECCCC
STVSPIELENAIRGRAETGFGVMDEQQLKIAVAQARQRGEKVVMTNGIFDILHAGHVSYL
CCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHH
ANARKLGDRLIVAVNSDASTKRLKGEKRPVNPLEQRMVVLGALEAVDWVVPFEEDTPQRL
HHHHHCCCEEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCHHHH
IADILPDLLVKGGDYKPHEIAGSEEVWAAGGEVKVLNFEDGVSTTNIIQSIKNGRG
HHHHHHHHHHCCCCCCCHHCCCCCCEEECCCEEEEEEECCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MKVTLPDFRRAGVLVVGDVMLDRYWYGPTCRISPEAPVPVVKVDTIEERPGGAANVAMNI
CCCCCCCHHHCCEEEEEHHHHHHEECCCCEEECCCCCCCEEEEECCCCCCCCHHHHHHHH
ASLGAVARLVGLTGIDDAARALICKLSEVRVRCDFVSVPTHPTITKLRVLSRNQQLIRLD
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHCCCCEEEEEE
FEEGFDGVDPTPIFERIQLALPQIGALVLSDYAKGALNSVQPMIQLARKANVPVLIDPKG
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
SDFERYRGATLLTPNLSEFEAVVGRCKNEEELVNRGMQLVADFELSALLVTRSEQGMTLL
CCHHHHCCCEEECCCHHHHHHHHHCCCCHHHHHHCCHHEEECCCEEEEEEEECCCCCEEE
QLGKPPLHLPTQAKEVFDVTGAGDTVIGVLAAALAAGNSLEESCFLANAAAGVVVGKLGT
ECCCCCCCCCCCHHHHEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEECCCC
STVSPIELENAIRGRAETGFGVMDEQQLKIAVAQARQRGEKVVMTNGIFDILHAGHVSYL
CCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHH
ANARKLGDRLIVAVNSDASTKRLKGEKRPVNPLEQRMVVLGALEAVDWVVPFEEDTPQRL
HHHHHCCCEEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCHHHH
IADILPDLLVKGGDYKPHEIAGSEEVWAAGGEVKVLNFEDGVSTTNIIQSIKNGRG
HHHHHHHHHHCCCCCCCHHCCCCCCEEECCCEEEEEEECCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA