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The map label for this gene is murE

Identifier: 218927746

GI number: 218927746

Start: 593654

End: 595126

Strand: Direct

Name: murE

Synonym: YPO0550

Alternate gene names: 218927746

Gene position: 593654-595126 (Clockwise)

Preceding gene: 218927745

Following gene: 218927747

Centisome position: 12.76

GC content: 53.84

Gene sequence:

>1473_bases
TTGCGCGACTTATTCGCTCCTTGGGGGCTAGACGTCCCGGAGCGGGCGCTACGGGAAATGACATTAGACAGCCGTATTGC
AGCTGCCGGCGATTTATTTGTTGCGGTTGTTGGCCACCAGACGGATGGGCGTCGCTACATCCCGCAAGCTATCGCCCAGG
GTGTGGCTGCAATTGTGGCCGAAGCCGATGGTGTCGCACCGGATGTCAGCGTTGCCGAGATACATGGCGTCCCTGTTATT
TATTTGCGTAATTTGAATCAGCACTTATCCACGCTGGCTGGGCAGTTTTACCATCAGCCTGGCGCCGCATTGCGTTTGGT
CGGTGTAACCGGAACGAATGGCAAAACCACCACTACGCAATTGTTAGCACAATGGAGCCAGGCGCTGGGCGAAACCAGTG
CAGTCATGGGCACCGTAGGTAATGGTTTATTAGGGCAGGTGATACCCACGGAGAACACCACCGGTTCTGCCGTAGATATT
CAACATTTGCTGCGTAATCTGGTTGATCAAGGTGCGACTTTTGCCGCCATGGAAGTTTCTTCCCACGGTTTGATTCAAGA
CCGTGTTGCTGCGTTATCCTTTGCAGCCGTAGTGTTCACTAACTTAAGCCGCGATCATCTGGATTATCACGGCAATATGA
CGAGCTATGAGGCGGCCAAATGGCTGCTGTTCTCCACTCATCAGTCCGAACACAAAATTATCAATGCTGATGATGACGTT
GGCCGCCGTTGGTTAAGCCAATTGCCGCAGGCTGTCGCCGTCAGCATGGCAGGCAATGTCCCCAAAGGCTGGAATGGCCC
GTGGTTATCTGCCAACAAGGTGATTTATCACGATAATGGTGCCAGCATCGCGTTTGATTCAAGCTGGGGCGAAGGCGAGC
TAGAAAGCCGTCTGATGGGCGCTTTCAACGTCAGTAACTTGTTGGTGGCATTAGCGACGTTACTGGTTCAGGGGTATCCC
ATGGCGCAGTTGTTAGCTGCAGCGCCTCATCTGCAACCGGTTTGTGGGCGTATGGAAGTGTTTAATGCGCCGGGTAAACC
AACCGTTGTGGTGGATTATGCCCACACGCCAGATGCATTAGAAAAAGCCTTGGCCGCCGCCCGTTTACATTGCACCGGCA
AGCTGTGGTGTGTGTTTGGCTGTGGCGGTGACCGTGATAAAGGTAAGCGCCCACTTATGGGCGGTATCGCAGAACAACTG
GCTGACTGTGTCGTGGTGACCGATGATAACCCACGCAGTGAAGAGCCACAGGCGATTGTTGCTGACATTCTCAGTGGCTT
GCTGGACGCGGGGCGTGTGCAGGCGATACATGGCCGTGCCGAAGCCGTAACCAGTGCCATTATGCAAGCCAAAGAAGACG
ACGTTGTCCTGATTGCCGGTAAAGGCCATGAAGATTATCAATTAGTTGGCAATCGTCGCTTGGATTATTCAGACCGTGTC
ACCGTCGCGCGTTTGTTAGGGGGGCAGGCATGA

Upstream 100 bases:

>100_bases
GTTTTACGCACCATGAACATTGAGCCAGATGCGTTACCCACCGGTGATAAAAGCGAATTAGTGATTAATACAAAAGAGGG
TTCAGGTGGCAGATCGTAAT

Downstream 100 bases:

>100_bases
TTAAGGTCTCTCTGCGTTTTCTGGCTAGCTTACTTAATGCTGAATATATCGTTGCTGAATCAGTCAGCGATGATATTGAG
ATAACTGAAGTGACCATTGA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MRDLFAPWGLDVPERALREMTLDSRIAAAGDLFVAVVGHQTDGRRYIPQAIAQGVAAIVAEADGVAPDVSVAEIHGVPVI
YLRNLNQHLSTLAGQFYHQPGAALRLVGVTGTNGKTTTTQLLAQWSQALGETSAVMGTVGNGLLGQVIPTENTTGSAVDI
QHLLRNLVDQGATFAAMEVSSHGLIQDRVAALSFAAVVFTNLSRDHLDYHGNMTSYEAAKWLLFSTHQSEHKIINADDDV
GRRWLSQLPQAVAVSMAGNVPKGWNGPWLSANKVIYHDNGASIAFDSSWGEGELESRLMGAFNVSNLLVALATLLVQGYP
MAQLLAAAPHLQPVCGRMEVFNAPGKPTVVVDYAHTPDALEKALAAARLHCTGKLWCVFGCGGDRDKGKRPLMGGIAEQL
ADCVVVTDDNPRSEEPQAIVADILSGLLDAGRVQAIHGRAEAVTSAIMQAKEDDVVLIAGKGHEDYQLVGNRRLDYSDRV
TVARLLGGQA

Sequences:

>Translated_490_residues
MRDLFAPWGLDVPERALREMTLDSRIAAAGDLFVAVVGHQTDGRRYIPQAIAQGVAAIVAEADGVAPDVSVAEIHGVPVI
YLRNLNQHLSTLAGQFYHQPGAALRLVGVTGTNGKTTTTQLLAQWSQALGETSAVMGTVGNGLLGQVIPTENTTGSAVDI
QHLLRNLVDQGATFAAMEVSSHGLIQDRVAALSFAAVVFTNLSRDHLDYHGNMTSYEAAKWLLFSTHQSEHKIINADDDV
GRRWLSQLPQAVAVSMAGNVPKGWNGPWLSANKVIYHDNGASIAFDSSWGEGELESRLMGAFNVSNLLVALATLLVQGYP
MAQLLAAAPHLQPVCGRMEVFNAPGKPTVVVDYAHTPDALEKALAAARLHCTGKLWCVFGCGGDRDKGKRPLMGGIAEQL
ADCVVVTDDNPRSEEPQAIVADILSGLLDAGRVQAIHGRAEAVTSAIMQAKEDDVVLIAGKGHEDYQLVGNRRLDYSDRV
TVARLLGGQA
>Mature_490_residues
MRDLFAPWGLDVPERALREMTLDSRIAAAGDLFVAVVGHQTDGRRYIPQAIAQGVAAIVAEADGVAPDVSVAEIHGVPVI
YLRNLNQHLSTLAGQFYHQPGAALRLVGVTGTNGKTTTTQLLAQWSQALGETSAVMGTVGNGLLGQVIPTENTTGSAVDI
QHLLRNLVDQGATFAAMEVSSHGLIQDRVAALSFAAVVFTNLSRDHLDYHGNMTSYEAAKWLLFSTHQSEHKIINADDDV
GRRWLSQLPQAVAVSMAGNVPKGWNGPWLSANKVIYHDNGASIAFDSSWGEGELESRLMGAFNVSNLLVALATLLVQGYP
MAQLLAAAPHLQPVCGRMEVFNAPGKPTVVVDYAHTPDALEKALAAARLHCTGKLWCVFGCGGDRDKGKRPLMGGIAEQL
ADCVVVTDDNPRSEEPQAIVADILSGLLDAGRVQAIHGRAEAVTSAIMQAKEDDVVLIAGKGHEDYQLVGNRRLDYSDRV
TVARLLGGQA

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=487, Percent_Identity=77.4127310061602, Blast_Score=734, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_YERPE (Q8ZIF4)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AC0068
- RefSeq:   NP_670928.1
- RefSeq:   NP_994905.1
- RefSeq:   YP_002345621.1
- ProteinModelPortal:   Q8ZIF4
- SMR:   Q8ZIF4
- GeneID:   1148578
- GeneID:   1173394
- GeneID:   2763730
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO0550
- KEGG:   ypk:y3631
- KEGG:   ypm:YP_3634
- HOGENOM:   HBG602753
- OMA:   GHEEGQT
- ProtClustDB:   PRK00139
- BioCyc:   YPES187410:Y3631-MONOMER
- BioCyc:   YPES214092:YPO0550-MONOMER
- BRENDA:   6.3.2.13
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.13

Molecular weight: Translated: 52236; Mature: 52236

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00228 TUBULIN_B_AUTOREG

Important sites: BINDING 22-22 BINDING 24-24 BINDING 152-152 BINDING 180-180 BINDING 186-186 BINDING 188-188 BINDING 385-385 BINDING 460-460 BINDING 464-464

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDLFAPWGLDVPERALREMTLDSRIAAAGDLFVAVVGHQTDGRRYIPQAIAQGVAAIVA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHH
EADGVAPDVSVAEIHGVPVIYLRNLNQHLSTLAGQFYHQPGAALRLVGVTGTNGKTTTTQ
HCCCCCCCCCHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHH
LLAQWSQALGETSAVMGTVGNGLLGQVIPTENTTGSAVDIQHLLRNLVDQGATFAAMEVS
HHHHHHHHHCCHHHHHHHCCCCCEECEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEECC
SHGLIQDRVAALSFAAVVFTNLSRDHLDYHGNMTSYEAAKWLLFSTHQSEHKIINADDDV
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHEEEEEECCCCCCCEECCCHHH
GRRWLSQLPQAVAVSMAGNVPKGWNGPWLSANKVIYHDNGASIAFDSSWGEGELESRLMG
HHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCEEEECCCCCCCHHHHHHHH
AFNVSNLLVALATLLVQGYPMAQLLAAAPHLQPVCGRMEVFNAPGKPTVVVDYAHTPDAL
HHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHCCHHEECCCCCCEEEEEECCCHHHH
EKALAAARLHCTGKLWCVFGCGGDRDKGKRPLMGGIAEQLADCVVVTDDNPRSEEPQAIV
HHHHHHHHHEECCEEEEEEECCCCCCCCCCCHHHHHHHHHHCEEEEECCCCCCCCHHHHH
ADILSGLLDAGRVQAIHGRAEAVTSAIMQAKEDDVVLIAGKGHEDYQLVGNRRLDYSDRV
HHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHCCCCCCCCCHH
TVARLLGGQA
HHHHHHCCCC
>Mature Secondary Structure
MRDLFAPWGLDVPERALREMTLDSRIAAAGDLFVAVVGHQTDGRRYIPQAIAQGVAAIVA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHH
EADGVAPDVSVAEIHGVPVIYLRNLNQHLSTLAGQFYHQPGAALRLVGVTGTNGKTTTTQ
HCCCCCCCCCHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHH
LLAQWSQALGETSAVMGTVGNGLLGQVIPTENTTGSAVDIQHLLRNLVDQGATFAAMEVS
HHHHHHHHHCCHHHHHHHCCCCCEECEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEECC
SHGLIQDRVAALSFAAVVFTNLSRDHLDYHGNMTSYEAAKWLLFSTHQSEHKIINADDDV
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHEEEEEECCCCCCCEECCCHHH
GRRWLSQLPQAVAVSMAGNVPKGWNGPWLSANKVIYHDNGASIAFDSSWGEGELESRLMG
HHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCEEEECCCCCCCHHHHHHHH
AFNVSNLLVALATLLVQGYPMAQLLAAAPHLQPVCGRMEVFNAPGKPTVVVDYAHTPDAL
HHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHCCHHEECCCCCCEEEEEECCCHHHH
EKALAAARLHCTGKLWCVFGCGGDRDKGKRPLMGGIAEQLADCVVVTDDNPRSEEPQAIV
HHHHHHHHHEECCEEEEEEECCCCCCCCCCCHHHHHHHHHHCEEEEECCCCCCCCHHHHH
ADILSGLLDAGRVQAIHGRAEAVTSAIMQAKEDDVVLIAGKGHEDYQLVGNRRLDYSDRV
HHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHCCCCCCCCCHH
TVARLLGGQA
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430