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The map label for this gene is mraW [H]

Identifier: 218927743

GI number: 218927743

Start: 590547

End: 591509

Strand: Direct

Name: mraW [H]

Synonym: YPO0547

Alternate gene names: 218927743

Gene position: 590547-591509 (Clockwise)

Preceding gene: 218927742

Following gene: 218927744

Centisome position: 12.69

GC content: 51.92

Gene sequence:

>963_bases
ATGGTAGACAACAACAAAACGGTAGATAACAACTACAAACATACCAGTGTATTGCTGGATGAGGCCGTAAAGGGCCTGAA
TATCCGTGATAACGGTATCTACATTGACGGCACTTTTGGCCGCGGCGGCCATTCGCGTTTGATTTTGTCCCAACTTGGGC
CAGAAGGGCGCTTAATTGCAATTGATCGGGATCCAGAAGCTATCGAAGCGGCGAAACAGATCACCGACCCCCGTTTCTCT
ATCGTACATGGTCCTTTTTCTGATTTAGCACATTATGTGCGGGATCTGGATCTCGTTGGCCGTATTGACGGCATATTGCT
GGATTTGGGCGTTTCATCACCACAACTTGATGATGCTGAACGCGGCTTCTCTTTTATGCGTGATGGGCCGCTGGATATGC
GGATGGACCCTTCTCGTGGCCTGTCTGCTGCTGAATGGCTGATGAAAGCCAGCGCTGATGACATTGCCTGGGTGCTGAAA
ACCTTCGGCGAAGAGCGCTTTGCCAAGCGTTTGGCCAAGGCGATTGTTGAGCGTAACCTCACTCAGCCCATGACACGGAC
CAAAGAGCTGGCTGACCTGATCGCTAATGCCAGTCCGTTTAGGGATAAGCATAAGCATCCAGCCACTCGCAGCTTCCAGG
CTATCCGTATTTACATTAACAGTGAATTGGAAGAGATCGAACGTGCACTCGATGGCGCGCACGAGGTTTTGGCGCCTGAA
GGCCGTTTGTCAGTGATCAGCTTCCACTCCCTTGAAGATCGTATTGTGAAAAACTTTATTCGTCATCATAGCCGTGGGCC
ACAGGTCCCCGCAGGCTTGCCATTGACGGAGGCACAACTGCGCAGCATGGGAGGGCGCACACTGAAATCGGTTGGCAAAA
TGATGCCAGGGGATGCAGAAATTGCTGAAAACCCACGCGCTCGCAGCTCGGTACTCCGTTTTGCTGAGAGGATCGGTGAG
TGA

Upstream 100 bases:

>100_bases
ATGAACAGACCTGGTATCAACAAGTCAAGGATGACATCGACGCAGAACAGTCAACTCAGGAACCTTTGTCTGAGCGGCTA
CAGGGCTTATCGCTATAAGT

Downstream 100 bases:

>100_bases
TAGGCAACGAGCGTCATGGTTTAGTCGGGGTCATTGGGGCAGATCTTATCCGCAATGCCAAAATCCCATTAATCCTACTG
GTCGCGGTGTTGATCTCTGC

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MVDNNKTVDNNYKHTSVLLDEAVKGLNIRDNGIYIDGTFGRGGHSRLILSQLGPEGRLIAIDRDPEAIEAAKQITDPRFS
IVHGPFSDLAHYVRDLDLVGRIDGILLDLGVSSPQLDDAERGFSFMRDGPLDMRMDPSRGLSAAEWLMKASADDIAWVLK
TFGEERFAKRLAKAIVERNLTQPMTRTKELADLIANASPFRDKHKHPATRSFQAIRIYINSELEEIERALDGAHEVLAPE
GRLSVISFHSLEDRIVKNFIRHHSRGPQVPAGLPLTEAQLRSMGGRTLKSVGKMMPGDAEIAENPRARSSVLRFAERIGE

Sequences:

>Translated_320_residues
MVDNNKTVDNNYKHTSVLLDEAVKGLNIRDNGIYIDGTFGRGGHSRLILSQLGPEGRLIAIDRDPEAIEAAKQITDPRFS
IVHGPFSDLAHYVRDLDLVGRIDGILLDLGVSSPQLDDAERGFSFMRDGPLDMRMDPSRGLSAAEWLMKASADDIAWVLK
TFGEERFAKRLAKAIVERNLTQPMTRTKELADLIANASPFRDKHKHPATRSFQAIRIYINSELEEIERALDGAHEVLAPE
GRLSVISFHSLEDRIVKNFIRHHSRGPQVPAGLPLTEAQLRSMGGRTLKSVGKMMPGDAEIAENPRARSSVLRFAERIGE
>Mature_320_residues
MVDNNKTVDNNYKHTSVLLDEAVKGLNIRDNGIYIDGTFGRGGHSRLILSQLGPEGRLIAIDRDPEAIEAAKQITDPRFS
IVHGPFSDLAHYVRDLDLVGRIDGILLDLGVSSPQLDDAERGFSFMRDGPLDMRMDPSRGLSAAEWLMKASADDIAWVLK
TFGEERFAKRLAKAIVERNLTQPMTRTKELADLIANASPFRDKHKHPATRSFQAIRIYINSELEEIERALDGAHEVLAPE
GRLSVISFHSLEDRIVKNFIRHHSRGPQVPAGLPLTEAQLRSMGGRTLKSVGKMMPGDAEIAENPRARSSVLRFAERIGE

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=347, Percent_Identity=36.0230547550432, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI165377202, Length=192, Percent_Identity=40.1041666666667, Blast_Score=128, Evalue=6e-30,
Organism=Escherichia coli, GI1786270, Length=307, Percent_Identity=77.1986970684039, Blast_Score=498, Evalue=1e-142,
Organism=Drosophila melanogaster, GI62472493, Length=328, Percent_Identity=39.6341463414634, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI28571637, Length=328, Percent_Identity=39.6341463414634, Blast_Score=197, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 35591; Mature: 35591

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDNNKTVDNNYKHTSVLLDEAVKGLNIRDNGIYIDGTFGRGGHSRLILSQLGPEGRLIA
CCCCCCCCCCCCHHHHHHHHHHHHCCCEECCCEEEECCCCCCCCCEEHHHHCCCCCCEEE
IDRDPEAIEAAKQITDPRFSIVHGPFSDLAHYVRDLDLVGRIDGILLDLGVSSPQLDDAE
ECCCCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCHH
RGFSFMRDGPLDMRMDPSRGLSAAEWLMKASADDIAWVLKTFGEERFAKRLAKAIVERNL
HCHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TQPMTRTKELADLIANASPFRDKHKHPATRSFQAIRIYINSELEEIERALDGAHEVLAPE
CCHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCC
GRLSVISFHSLEDRIVKNFIRHHSRGPQVPAGLPLTEAQLRSMGGRTLKSVGKMMPGDAE
CCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHCCCCCCH
IAENPRARSSVLRFAERIGE
HHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVDNNKTVDNNYKHTSVLLDEAVKGLNIRDNGIYIDGTFGRGGHSRLILSQLGPEGRLIA
CCCCCCCCCCCCHHHHHHHHHHHHCCCEECCCEEEECCCCCCCCCEEHHHHCCCCCCEEE
IDRDPEAIEAAKQITDPRFSIVHGPFSDLAHYVRDLDLVGRIDGILLDLGVSSPQLDDAE
ECCCCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCHH
RGFSFMRDGPLDMRMDPSRGLSAAEWLMKASADDIAWVLKTFGEERFAKRLAKAIVERNL
HCHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TQPMTRTKELADLIANASPFRDKHKHPATRSFQAIRIYINSELEEIERALDGAHEVLAPE
CCHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCC
GRLSVISFHSLEDRIVKNFIRHHSRGPQVPAGLPLTEAQLRSMGGRTLKSVGKMMPGDAE
CCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHCCCCCCH
IAENPRARSSVLRFAERIGE
HHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA