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The map label for this gene is leuO [H]

Identifier: 218927732

GI number: 218927732

Start: 580264

End: 581220

Strand: Direct

Name: leuO [H]

Synonym: YPO0535

Alternate gene names: 218927732

Gene position: 580264-581220 (Clockwise)

Preceding gene: 218927724

Following gene: 218927733

Centisome position: 12.47

GC content: 42.63

Gene sequence:

>957_bases
ATGTCTGAATACAACTTAGTAACTGACAGTCAAGCAACTAAAAAAGAATCTATCGATGTCCATTTAAGAAGTGTCGACCT
AAATTTGTTAACTGTTTTCGATGCTGTGATGCAAATGCAAAATGTGACACGTGCGGCACAGTCTCTTGGTATGTCCCAGC
CTGCTGTAAGTAATGCTGTTGCCCGTTTGAAAGTCATGTTCAATGATGAGCTCTTTGTCCGTTATGGTCGTGGCATACAG
CCTACAGCCAGAGCCCGTCAGCTCTTCGGGCCGGTACGCCAGGCATTGCAGTTAGTACAAAATGAGTTACCCGGTGCAGG
GTTTGAAGCGAAAAACAGTGAACGGCTGTTTAATCTATCGATTTGTAGCCCGTTAGATGTTCGACTGGCGGCTCAGATTG
TACAGCGGGTAAAACAATCAGCGCCTAATGTTCAACTGGTCATCAAATCTTATCTTAATGACAACATTGAGCATCAACTA
CGTTATCAGGAGACCGAGTTTGTCATCGGCTACACCGAGTTCGACCGCCCGGATTTTCACCGCATCCCTTTATTTAAAGA
TGAATTAGTGCTGGCCGTTTCTAAAGATCACCCACGTATTAATGATTCAATTACTGAAAATCAGCTCCTTTCCGAATCTC
ACGCGGTTGTATCATTAGATAAAAAAGGGTCATTCAGTGAGCCTTATTACGACTCAACAACCAGTACTCAATCTATTGCT
TATGAAAGTACTGATATGAACAGTGTGCTAAATATTGTTTCTCAGACTTATTTTGTGGCAATTGCTCCCCGCTGGTTGGT
AAAAACATACAGTGAGGCGTTGAATCTAAAGTTAATTTCATTACCTTGGGAAAATACTTCGCGCGCCTGTTACCTTACAT
GGCATGAATCTACGGATAGAGATAAAGGCCATCAATGGATGAAAGCGTTACTCGGGCAATTTAGTTCTCCCGTTTAA

Upstream 100 bases:

>100_bases
AGTGAGCGGCACGAGCAACGTTGAAGAATAAGGGTATATCAATATCAGAGAAGTCCTTTTATTAATCGCGCCATCTGCAT
AAAGCTTAGTGGAGTTAAGC

Downstream 100 bases:

>100_bases
TGATTTATCATTAAGTTCTGATCATTAAGTCCTGCTGCTGAGTTCATCTTGTTATTGGTAGCAGGCAATCATTCACCTAA
CCATATCTCATATTTTCATC

Product: leucine transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MSEYNLVTDSQATKKESIDVHLRSVDLNLLTVFDAVMQMQNVTRAAQSLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQ
PTARARQLFGPVRQALQLVQNELPGAGFEAKNSERLFNLSICSPLDVRLAAQIVQRVKQSAPNVQLVIKSYLNDNIEHQL
RYQETEFVIGYTEFDRPDFHRIPLFKDELVLAVSKDHPRINDSITENQLLSESHAVVSLDKKGSFSEPYYDSTTSTQSIA
YESTDMNSVLNIVSQTYFVAIAPRWLVKTYSEALNLKLISLPWENTSRACYLTWHESTDRDKGHQWMKALLGQFSSPV

Sequences:

>Translated_318_residues
MSEYNLVTDSQATKKESIDVHLRSVDLNLLTVFDAVMQMQNVTRAAQSLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQ
PTARARQLFGPVRQALQLVQNELPGAGFEAKNSERLFNLSICSPLDVRLAAQIVQRVKQSAPNVQLVIKSYLNDNIEHQL
RYQETEFVIGYTEFDRPDFHRIPLFKDELVLAVSKDHPRINDSITENQLLSESHAVVSLDKKGSFSEPYYDSTTSTQSIA
YESTDMNSVLNIVSQTYFVAIAPRWLVKTYSEALNLKLISLPWENTSRACYLTWHESTDRDKGHQWMKALLGQFSSPV
>Mature_317_residues
SEYNLVTDSQATKKESIDVHLRSVDLNLLTVFDAVMQMQNVTRAAQSLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQP
TARARQLFGPVRQALQLVQNELPGAGFEAKNSERLFNLSICSPLDVRLAAQIVQRVKQSAPNVQLVIKSYLNDNIEHQLR
YQETEFVIGYTEFDRPDFHRIPLFKDELVLAVSKDHPRINDSITENQLLSESHAVVSLDKKGSFSEPYYDSTTSTQSIAY
ESTDMNSVLNIVSQTYFVAIAPRWLVKTYSEALNLKLISLPWENTSRACYLTWHESTDRDKGHQWMKALLGQFSSPV

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081684, Length=290, Percent_Identity=64.8275862068965, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI1786820, Length=282, Percent_Identity=25.886524822695, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI87081766, Length=273, Percent_Identity=24.9084249084249, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: 825 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36067; Mature: 35936

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEYNLVTDSQATKKESIDVHLRSVDLNLLTVFDAVMQMQNVTRAAQSLGMSQPAVSNAV
CCCCCCCCCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
ARLKVMFNDELFVRYGRGIQPTARARQLFGPVRQALQLVQNELPGAGFEAKNSERLFNLS
HHEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEE
ICSPLDVRLAAQIVQRVKQSAPNVQLVIKSYLNDNIEHQLRYQETEFVIGYTEFDRPDFH
CCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHCCCHHHHHEECCCEEEEEEECCCCCCCC
RIPLFKDELVLAVSKDHPRINDSITENQLLSESHAVVSLDKKGSFSEPYYDSTTSTQSIA
CCCCCCCCEEEEEECCCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCEE
YESTDMNSVLNIVSQTYFVAIAPRWLVKTYSEALNLKLISLPWENTSRACYLTWHESTDR
ECCCCHHHHHHHHHHHHEEEECHHHHHHHHHHHHCEEEEEECCCCCCCEEEEEECCCCCC
DKGHQWMKALLGQFSSPV
CHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SEYNLVTDSQATKKESIDVHLRSVDLNLLTVFDAVMQMQNVTRAAQSLGMSQPAVSNAV
CCCCCCCCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
ARLKVMFNDELFVRYGRGIQPTARARQLFGPVRQALQLVQNELPGAGFEAKNSERLFNLS
HHEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEE
ICSPLDVRLAAQIVQRVKQSAPNVQLVIKSYLNDNIEHQLRYQETEFVIGYTEFDRPDFH
CCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHCCCHHHHHEECCCEEEEEEECCCCCCCC
RIPLFKDELVLAVSKDHPRINDSITENQLLSESHAVVSLDKKGSFSEPYYDSTTSTQSIA
CCCCCCCCEEEEEECCCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCEE
YESTDMNSVLNIVSQTYFVAIAPRWLVKTYSEALNLKLISLPWENTSRACYLTWHESTDR
ECCCCHHHHHHHHHHHHEEEECHHHHHHHHHHHHCEEEEEECCCCCCCEEEEEECCCCCC
DKGHQWMKALLGQFSSPV
CHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 3519576 [H]