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The map label for this gene is prfC [H]

Identifier: 218927631

GI number: 218927631

Start: 449865

End: 451454

Strand: Direct

Name: prfC [H]

Synonym: YPO0430

Alternate gene names: 218927631

Gene position: 449865-451454 (Clockwise)

Preceding gene: 218927630

Following gene: 218927632

Centisome position: 9.67

GC content: 49.75

Gene sequence:

>1590_bases
ATGTCTCCTAGTGAATATGCTCTGGAAGTCGCGAAAAGACGTACTTTTGCGATCATTTCCCACCCCGATGCCGGTAAAAC
AACCATTACCGAAAAAGTGTTGCTGTTCGGACATGCAATTCAGACTGCCGGGACAGTAAAAGGCCGTGGTTCAAGCCATC
ATGCCAAATCTGACTGGATGGAAATGGAAAAACAACGCGGTATCTCCATTACCACGTCGGTAATGCAGTTTCCGTATGGC
GGCTGTCTGGTCAATCTGCTTGATACCCCAGGGCATGAAGACTTCTCCGAAGATACGTACCGCACACTGACGGCGGTTGA
CTGCTGTTTGATGGTGATCGATGCGGCCAAAGGGGTAGAAGACCGGACCCGCAAATTGATGGAAGTGACCCGTCTGCGCG
ATACGCCAATTCTGACCTTTATGAACAAATTGGACCGCGAAATTCGTGATCCAATGGAAGTTCTGGATGAAGTCGAGCGT
GAGCTGAACATTGCCTGTTCACCTATCACTTGGCCGATTGGTTGCGGTAAGTCGTTTAAAGGGGTTTATCACCTTCATAA
AGACGAAACTTATCTGTATCAAAGCGGTAAAGGCCATACCATTCAGGAAGTCCGTATCGTTAAAGGGCTGAACAACCCAG
ATCTGGATGTGGCTGTTGGCGAAGACTTGGCCAAGCAGTTCCGTCAGGAGTTGGAACTGGTTCAGGGCGCTTCCCACGAG
TTTGATCATGAGGCTTTCCTGTCCGGTGATTTAACGCCCGTCTTCTTCGGTACTGCGTTGGGTAACTTCGGTGTGGACCA
TATGCTAGATGGCTTGGTCGAATGGGCACCGGCACCGATGCCGCGTAAAACGGATACCCGCGTAGTGGTCGCCTCTGAAG
AGAAATTCACCGGTTTCGTTTTCAAAATTCAGGCCAATATGGATCCGAAACACCGTGACCGTGTGGCCTTTATGCGCGTG
GTGTCTGGCCGTTTTGAGAAAGGGATGAAGCTGCGTCAGGTACGGACCAAGAAAGACGTGGTGATTTCAGACGCGCTGAC
CTTTATGGCCGGTGACCGTTCTCACGTTGAAGAGGCCTATGCTGGTGATATTATCGGCTTGCATAACCATGGCACCATCC
AGATCGGTGACACCTTCACTCAAGGTGAAGATATGAAATTCACCGGTATTCCTAACTTTGCCCCTGAATTGTTCCGCCGC
ATTCGCCTGCGCGATCCATTAAAGCAGAAGCAGTTGTTAAAAGGCTTGGTTCAGTTGTCAGAAGAGGGAGCCGTTCAGGT
ATTCCGCCCACTGAGCAACAACGATTTGATCGTGGGCGCTGTGGGGGTGCTGCAGTTTGAAGTGGTCTCTTCCCGCTTGA
AGAGTGAATACAACGTCGAAGCGGTGTATGAATCGGTTAACGTTTCTACCGCGCGTTGGGTCGAATGTCATGACGTGAAG
AAATTTGAGGAGTTCAAACGTAAGAATGAGCTTAATTTGGCCTTAGACGGTGGCGATAATTTAAGTTACATCGCGCCCAC
GATGGTTAATCTCAATATTACACAAGAGCGTTACCCGGACGTTATTTTCCGCAAAACACGCGAGCACTAA

Upstream 100 bases:

>100_bases
GAAAATATCGCGCAATAATCAATGTCTCGCTTTGCCGGACCCACATAGCAATAAAATGTGAGGCTCAGAGCAGGGCGGCC
TAAAAGTAGAAACTTCAATT

Downstream 100 bases:

>100_bases
GTTTATTGCTAACGAACCTGCTGCAAGCCGCATTCTATCGGGGTATCTATTACCCCGAGTCATCAATCTTATTCGATATT
TTTTCCTAGGATAATTCCTA

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MSPSEYALEVAKRRTFAIISHPDAGKTTITEKVLLFGHAIQTAGTVKGRGSSHHAKSDWMEMEKQRGISITTSVMQFPYG
GCLVNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDREIRDPMEVLDEVER
ELNIACSPITWPIGCGKSFKGVYHLHKDETYLYQSGKGHTIQEVRIVKGLNNPDLDVAVGEDLAKQFRQELELVQGASHE
FDHEAFLSGDLTPVFFGTALGNFGVDHMLDGLVEWAPAPMPRKTDTRVVVASEEKFTGFVFKIQANMDPKHRDRVAFMRV
VSGRFEKGMKLRQVRTKKDVVISDALTFMAGDRSHVEEAYAGDIIGLHNHGTIQIGDTFTQGEDMKFTGIPNFAPELFRR
IRLRDPLKQKQLLKGLVQLSEEGAVQVFRPLSNNDLIVGAVGVLQFEVVSSRLKSEYNVEAVYESVNVSTARWVECHDVK
KFEEFKRKNELNLALDGGDNLSYIAPTMVNLNITQERYPDVIFRKTREH

Sequences:

>Translated_529_residues
MSPSEYALEVAKRRTFAIISHPDAGKTTITEKVLLFGHAIQTAGTVKGRGSSHHAKSDWMEMEKQRGISITTSVMQFPYG
GCLVNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDREIRDPMEVLDEVER
ELNIACSPITWPIGCGKSFKGVYHLHKDETYLYQSGKGHTIQEVRIVKGLNNPDLDVAVGEDLAKQFRQELELVQGASHE
FDHEAFLSGDLTPVFFGTALGNFGVDHMLDGLVEWAPAPMPRKTDTRVVVASEEKFTGFVFKIQANMDPKHRDRVAFMRV
VSGRFEKGMKLRQVRTKKDVVISDALTFMAGDRSHVEEAYAGDIIGLHNHGTIQIGDTFTQGEDMKFTGIPNFAPELFRR
IRLRDPLKQKQLLKGLVQLSEEGAVQVFRPLSNNDLIVGAVGVLQFEVVSSRLKSEYNVEAVYESVNVSTARWVECHDVK
KFEEFKRKNELNLALDGGDNLSYIAPTMVNLNITQERYPDVIFRKTREH
>Mature_528_residues
SPSEYALEVAKRRTFAIISHPDAGKTTITEKVLLFGHAIQTAGTVKGRGSSHHAKSDWMEMEKQRGISITTSVMQFPYGG
CLVNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDREIRDPMEVLDEVERE
LNIACSPITWPIGCGKSFKGVYHLHKDETYLYQSGKGHTIQEVRIVKGLNNPDLDVAVGEDLAKQFRQELELVQGASHEF
DHEAFLSGDLTPVFFGTALGNFGVDHMLDGLVEWAPAPMPRKTDTRVVVASEEKFTGFVFKIQANMDPKHRDRVAFMRVV
SGRFEKGMKLRQVRTKKDVVISDALTFMAGDRSHVEEAYAGDIIGLHNHGTIQIGDTFTQGEDMKFTGIPNFAPELFRRI
RLRDPLKQKQLLKGLVQLSEEGAVQVFRPLSNNDLIVGAVGVLQFEVVSSRLKSEYNVEAVYESVNVSTARWVECHDVKK
FEEFKRKNELNLALDGGDNLSYIAPTMVNLNITQERYPDVIFRKTREH

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=481, Percent_Identity=27.8586278586279, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI19923640, Length=511, Percent_Identity=25.440313111546, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI25306283, Length=394, Percent_Identity=26.9035532994924, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI25306287, Length=483, Percent_Identity=24.4306418219462, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI157426893, Length=169, Percent_Identity=28.9940828402367, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI94966754, Length=142, Percent_Identity=30.2816901408451, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1790835, Length=529, Percent_Identity=89.0359168241966, Blast_Score=987, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=493, Percent_Identity=25.1521298174442, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI48994988, Length=216, Percent_Identity=30.0925925925926, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788922, Length=151, Percent_Identity=33.112582781457, Blast_Score=86, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17533571, Length=500, Percent_Identity=27, Blast_Score=171, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI17556745, Length=179, Percent_Identity=31.2849162011173, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17557151, Length=134, Percent_Identity=29.8507462686567, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=485, Percent_Identity=27.2164948453608, Blast_Score=169, Evalue=8e-43,
Organism=Saccharomyces cerevisiae, GI6322359, Length=419, Percent_Identity=26.0143198090692, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6323320, Length=151, Percent_Identity=25.8278145695364, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24582462, Length=482, Percent_Identity=26.7634854771784, Blast_Score=169, Evalue=6e-42,
Organism=Drosophila melanogaster, GI221458488, Length=507, Percent_Identity=24.4575936883629, Blast_Score=125, Evalue=8e-29,
Organism=Drosophila melanogaster, GI78706572, Length=160, Percent_Identity=31.875, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24585709, Length=156, Percent_Identity=28.8461538461538, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24585711, Length=156, Percent_Identity=28.8461538461538, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24585713, Length=156, Percent_Identity=28.8461538461538, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59641; Mature: 59510

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPSEYALEVAKRRTFAIISHPDAGKTTITEKVLLFGHAIQTAGTVKGRGSSHHAKSDWM
CCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
EMEKQRGISITTSVMQFPYGGCLVNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVE
HHHHHCCCEEEHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHH
DRTRKLMEVTRLRDTPILTFMNKLDREIRDPMEVLDEVERELNIACSPITWPIGCGKSFK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCEEECCCEECCCCCCCCC
GVYHLHKDETYLYQSGKGHTIQEVRIVKGLNNPDLDVAVGEDLAKQFRQELELVQGASHE
CEEEEECCCEEEEECCCCCCHHHHHEECCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCC
FDHEAFLSGDLTPVFFGTALGNFGVDHMLDGLVEWAPAPMPRKTDTRVVVASEEKFTGFV
CCCHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEE
FKIQANMDPKHRDRVAFMRVVSGRFEKGMKLRQVRTKKDVVISDALTFMAGDRSHVEEAY
EEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCEEHHHHHHHHCCCHHHHHHHH
AGDIIGLHNHGTIQIGDTFTQGEDMKFTGIPNFAPELFRRIRLRDPLKQKQLLKGLVQLS
CCCEEEECCCCEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHC
EEGAVQVFRPLSNNDLIVGAVGVLQFEVVSSRLKSEYNVEAVYESVNVSTARWVECHDVK
CCCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEEHHHHH
KFEEFKRKNELNLALDGGDNLSYIAPTMVNLNITQERYPDVIFRKTREH
HHHHHHCCCCCEEEEECCCCCEEECCEEEEEEECHHHCCHHHHHHCCCC
>Mature Secondary Structure 
SPSEYALEVAKRRTFAIISHPDAGKTTITEKVLLFGHAIQTAGTVKGRGSSHHAKSDWM
CCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
EMEKQRGISITTSVMQFPYGGCLVNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVE
HHHHHCCCEEEHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHH
DRTRKLMEVTRLRDTPILTFMNKLDREIRDPMEVLDEVERELNIACSPITWPIGCGKSFK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCEEECCCEECCCCCCCCC
GVYHLHKDETYLYQSGKGHTIQEVRIVKGLNNPDLDVAVGEDLAKQFRQELELVQGASHE
CEEEEECCCEEEEECCCCCCHHHHHEECCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCC
FDHEAFLSGDLTPVFFGTALGNFGVDHMLDGLVEWAPAPMPRKTDTRVVVASEEKFTGFV
CCCHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEE
FKIQANMDPKHRDRVAFMRVVSGRFEKGMKLRQVRTKKDVVISDALTFMAGDRSHVEEAY
EEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCEEHHHHHHHHCCCHHHHHHHH
AGDIIGLHNHGTIQIGDTFTQGEDMKFTGIPNFAPELFRRIRLRDPLKQKQLLKGLVQLS
CCCEEEECCCCEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHC
EEGAVQVFRPLSNNDLIVGAVGVLQFEVVSSRLKSEYNVEAVYESVNVSTARWVECHDVK
CCCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEEHHHHH
KFEEFKRKNELNLALDGGDNLSYIAPTMVNLNITQERYPDVIFRKTREH
HHHHHHCCCCCEEEEECCCCCEEECCEEEEEEECHHHCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA