Click here to switch to the map view.

The map label for this gene is ptsA [H]

Identifier: 218927607

GI number: 218927607

Start: 421758

End: 424316

Strand: Direct

Name: ptsA [H]

Synonym: YPO0405

Alternate gene names: 218927607

Gene position: 421758-424316 (Clockwise)

Preceding gene: 218927603

Following gene: 218927616

Centisome position: 9.06

GC content: 52.64

Gene sequence:

>2559_bases
ATGACCAAGCAAGTGACATTTATCTGCGGGCTTCTTAATGGCGTTCATGCCCGACCTGCGAGCCATATAGAAAGGGTATG
CAACCGCTTCCAATCGCGTTTCCACTGGTATAATCCGCGTTCTGGCATCGTGGGAGATGGCAAGAGCGTACTTTCGCTGA
TCGCGGCCAATATCCTGTTAGGGGATGAATGTCAGGTCACCATTGAAGGCGAAGATGAGCAGGTTGCTTTTGAACGCCTG
AGTATTTTTATTGAGCACGAGCTTCCACACGCTGACCCCATCTTGCCGAAGAGGGAGGAGAGTGCAGAATGGGAGCCGCT
CCCCGCCTCGTTAGCCCACTTGCACCCCACCCTGTTACGCGCCCGTTCAGTCAGTCCAGGTACCGCCTGCGGGAAGTTGC
TGTCTCTGATAAGAGCCGATCTCAATGCATTGGGTGATTTACCCGTGGCGCAAGGTATCGAGAGGGAGCAGCAGATGCTT
GCCGATGGGGTGGCGCAGCTAGGTAAAGCGTGGGAGAGCTTATTAGTCGCCAATAGCAGTACGGCAGCCAATAGCAGTAC
GACAGAAAATAACAGTACGACAGAAAATAACAGCACGACGCGAGCGATACGGGAAGTACACCGCTCGCTGTTGCGTGATG
GCACATTTCGTCAACGGCTATTGAGTCACATAGTAGCAGGTGAAAGCTGTGCGACAGCCATCGTCGCTACTGCGGCCTAT
TTCAGCCAGCAGTTGGCGCTTGCTGCTAACACTTATCTACGTGAACGTGAGTTGGATATTCGAGACGTCAGTTTTCAATT
GTTACAGCAGATTTATGGTGAGCAACGTTTTCCCTCCCAGCAAGCATTGAGCGAAGACAGTCTCTGCATTGCCGATGAGT
TAACCCCGAGCCAATTCCTGGCGCTGGATAAACGTTATCTAAAGGGGTTGCTATTGGGCCGTGGTGGCAGTACTTCACAT
ACGGTGATTCTGGCGCGTTCATTCAATATTCCGACGCTCGTGGGTGTCGATGCTGCGGCACTCCAGCCGTATATTAATCA
ATCGCTGCAGATTGATGGTGAGCTCGGGCTGGTGGTCTGTCTTCTGGATGAACCGGTACGCCGTTACTATCGGCAGGAGC
AGTGGCTACACGACCAACTGCGTGAGCAGCAGAGCCGATACCAGAATATGCCTGGGCGTACTCTTGATGGTGTCAGAATG
GTGGTGGCGGCTAACATCACCCATGCGGTAGAGGTGGAGGGGGCTTTCAATCAAGGTGCCGAATCCATTGGCTTGTTTCG
TACTGAAATATTGTATATGGATCGTGCAGCCGCGCCTTCAGAAGAGGAGCTTTATACCCTCTACGCTCAGGCACTTGGCG
CTGCCAAGGGCAAACCGATTATTATTCGCACCATCGATATTGGCGGTGATAAACCGGTGAGTTATCTGAATATTCCTGCG
GAGAGCAACCCGTTTCTGGGCTACCGCGCGGTGCGTATTTATCACGAGTTTCTGTCGCTATTTCATACTCAATTGCGGGC
GATCCTGCGGGCGTCAATGCATGGCCCGCTCAAAATCATGATCCCGATGATCTCCTCAATGGAGGAGATCCTGTGGGTGA
AAGATCAACTGGCAGAGGTGAAACAATCTCTGCGTATCAGTCATCTTCAGTTCGACGAAACTGTCCCCTTGGGTATGATG
CTGGAAGTGCCTTCAGTGATGTTCATCATCGATCAATGCTGTGAGGAAATGGATTTTTTGAGTATTGGCAGTAACGACCT
GACACAGTACCTGCTGGCTGTTGATCGCGATAATGCCAAGGTGAGTGAGCACTATCACTGCTTGCCCCCGGCACTCCTAC
GCGCTCTCGATTACGCGGTGTGCGAGGTACATCGCCACGGTAAGTGGATTGGCTTATGTGGTGAACTGGCGGCAAAAGAC
TCGGTATTACCTCTGTTGGTAGCGATGGGGCTAGATGAGATCAGCATGAGTGCCTCTTTTATTGGCGCGACTAAAGCTCG
CCTTGCCAAACTTGATCGCGGCGAGTGCCGCCTGCTGCTCAACCGGGTGATGGCTTGCCGCACATCACGGGAAGTAGAAT
ATCTGCTGGTGCAGTACGACGGGGAACAACGTGATGAACCACTGATTATTCCTGAATGTATTACTCTCGGCGCAGACTGG
CGTAGCAAAGAAGAGGTGATCAAAGGGATGGTGGATAACCTGCTGCTGGCTGGACGTTGCCGGTATCCGCGCAAACTGGC
TGCGGATGTGTGGGCGCGAGAAGCCCTTTTTTCCACTGGATTGGGCTTTGGGTTTGCCATTCCGCATGCTCAATCTGAGC
ATATTGAGCAGTCGACGATCAGCGTGGCCAAGTTGGCAAAACCGGTGAATTGGGGAGAGGAAGACGCACTGTTTGTGATG
ATGTTAACCCTGAACAAACAGGCTTCGGGTGACCAGCATATGCGTATTTTTTCCAAACTGGCGCGTCGGATCATGCACGA
GGGGTTCCGCGATGCGCTGGTTAGCGCTGGGACGGCCCAGCAAGTAGAAATGCTGCTGAAACACGAGCTCGAACTGTGA

Upstream 100 bases:

>100_bases
TGTGATTACATTTGTCCAGTATTTGACATTTTTATCTCATAGCCGCGAGGGGATAGCTGCTTTTATTCTGGTTGCCAGCC
AGCGAGGGGAGGAAAGCCCA

Downstream 100 bases:

>100_bases
TTCTTTCATTTCAACATGACGGGTGCCGCGTCTGAGGTGGCACCCAGTGACCCTTATCGGATTACTTCAATAAGAATGAC
TTTCCTTGCTTAAGCACTAA

Product: putative phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: MTP 2; Phosphoenolpyruvate-protein phosphotransferase; Enzyme I-Ani; Phosphotransferase system enzyme I; Phosphocarrier protein HPr; Protein H; Fructose-like phosphotransferase enzyme IIA component; PTS system fructose-like EIIA component [H]

Number of amino acids: Translated: 852; Mature: 851

Protein sequence:

>852_residues
MTKQVTFICGLLNGVHARPASHIERVCNRFQSRFHWYNPRSGIVGDGKSVLSLIAANILLGDECQVTIEGEDEQVAFERL
SIFIEHELPHADPILPKREESAEWEPLPASLAHLHPTLLRARSVSPGTACGKLLSLIRADLNALGDLPVAQGIEREQQML
ADGVAQLGKAWESLLVANSSTAANSSTTENNSTTENNSTTRAIREVHRSLLRDGTFRQRLLSHIVAGESCATAIVATAAY
FSQQLALAANTYLRERELDIRDVSFQLLQQIYGEQRFPSQQALSEDSLCIADELTPSQFLALDKRYLKGLLLGRGGSTSH
TVILARSFNIPTLVGVDAAALQPYINQSLQIDGELGLVVCLLDEPVRRYYRQEQWLHDQLREQQSRYQNMPGRTLDGVRM
VVAANITHAVEVEGAFNQGAESIGLFRTEILYMDRAAAPSEEELYTLYAQALGAAKGKPIIIRTIDIGGDKPVSYLNIPA
ESNPFLGYRAVRIYHEFLSLFHTQLRAILRASMHGPLKIMIPMISSMEEILWVKDQLAEVKQSLRISHLQFDETVPLGMM
LEVPSVMFIIDQCCEEMDFLSIGSNDLTQYLLAVDRDNAKVSEHYHCLPPALLRALDYAVCEVHRHGKWIGLCGELAAKD
SVLPLLVAMGLDEISMSASFIGATKARLAKLDRGECRLLLNRVMACRTSREVEYLLVQYDGEQRDEPLIIPECITLGADW
RSKEEVIKGMVDNLLLAGRCRYPRKLAADVWAREALFSTGLGFGFAIPHAQSEHIEQSTISVAKLAKPVNWGEEDALFVM
MLTLNKQASGDQHMRIFSKLARRIMHEGFRDALVSAGTAQQVEMLLKHELEL

Sequences:

>Translated_852_residues
MTKQVTFICGLLNGVHARPASHIERVCNRFQSRFHWYNPRSGIVGDGKSVLSLIAANILLGDECQVTIEGEDEQVAFERL
SIFIEHELPHADPILPKREESAEWEPLPASLAHLHPTLLRARSVSPGTACGKLLSLIRADLNALGDLPVAQGIEREQQML
ADGVAQLGKAWESLLVANSSTAANSSTTENNSTTENNSTTRAIREVHRSLLRDGTFRQRLLSHIVAGESCATAIVATAAY
FSQQLALAANTYLRERELDIRDVSFQLLQQIYGEQRFPSQQALSEDSLCIADELTPSQFLALDKRYLKGLLLGRGGSTSH
TVILARSFNIPTLVGVDAAALQPYINQSLQIDGELGLVVCLLDEPVRRYYRQEQWLHDQLREQQSRYQNMPGRTLDGVRM
VVAANITHAVEVEGAFNQGAESIGLFRTEILYMDRAAAPSEEELYTLYAQALGAAKGKPIIIRTIDIGGDKPVSYLNIPA
ESNPFLGYRAVRIYHEFLSLFHTQLRAILRASMHGPLKIMIPMISSMEEILWVKDQLAEVKQSLRISHLQFDETVPLGMM
LEVPSVMFIIDQCCEEMDFLSIGSNDLTQYLLAVDRDNAKVSEHYHCLPPALLRALDYAVCEVHRHGKWIGLCGELAAKD
SVLPLLVAMGLDEISMSASFIGATKARLAKLDRGECRLLLNRVMACRTSREVEYLLVQYDGEQRDEPLIIPECITLGADW
RSKEEVIKGMVDNLLLAGRCRYPRKLAADVWAREALFSTGLGFGFAIPHAQSEHIEQSTISVAKLAKPVNWGEEDALFVM
MLTLNKQASGDQHMRIFSKLARRIMHEGFRDALVSAGTAQQVEMLLKHELEL
>Mature_851_residues
TKQVTFICGLLNGVHARPASHIERVCNRFQSRFHWYNPRSGIVGDGKSVLSLIAANILLGDECQVTIEGEDEQVAFERLS
IFIEHELPHADPILPKREESAEWEPLPASLAHLHPTLLRARSVSPGTACGKLLSLIRADLNALGDLPVAQGIEREQQMLA
DGVAQLGKAWESLLVANSSTAANSSTTENNSTTENNSTTRAIREVHRSLLRDGTFRQRLLSHIVAGESCATAIVATAAYF
SQQLALAANTYLRERELDIRDVSFQLLQQIYGEQRFPSQQALSEDSLCIADELTPSQFLALDKRYLKGLLLGRGGSTSHT
VILARSFNIPTLVGVDAAALQPYINQSLQIDGELGLVVCLLDEPVRRYYRQEQWLHDQLREQQSRYQNMPGRTLDGVRMV
VAANITHAVEVEGAFNQGAESIGLFRTEILYMDRAAAPSEEELYTLYAQALGAAKGKPIIIRTIDIGGDKPVSYLNIPAE
SNPFLGYRAVRIYHEFLSLFHTQLRAILRASMHGPLKIMIPMISSMEEILWVKDQLAEVKQSLRISHLQFDETVPLGMML
EVPSVMFIIDQCCEEMDFLSIGSNDLTQYLLAVDRDNAKVSEHYHCLPPALLRALDYAVCEVHRHGKWIGLCGELAAKDS
VLPLLVAMGLDEISMSASFIGATKARLAKLDRGECRLLLNRVMACRTSREVEYLLVQYDGEQRDEPLIIPECITLGADWR
SKEEVIKGMVDNLLLAGRCRYPRKLAADVWAREALFSTGLGFGFAIPHAQSEHIEQSTISVAKLAKPVNWGEEDALFVMM
LTLNKQASGDQHMRIFSKLARRIMHEGFRDALVSAGTAQQVEMLLKHELEL

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane [H]

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI48994992, Length=848, Percent_Identity=62.5, Blast_Score=1059, Evalue=0.0,
Organism=Escherichia coli, GI1788726, Length=852, Percent_Identity=41.4319248826291, Blast_Score=616, Evalue=1e-177,
Organism=Escherichia coli, GI1788756, Length=453, Percent_Identity=38.1898454746137, Blast_Score=322, Evalue=8e-89,
Organism=Escherichia coli, GI1789193, Length=509, Percent_Identity=32.6129666011788, Blast_Score=249, Evalue=6e-67,
Organism=Escherichia coli, GI1787994, Length=411, Percent_Identity=26.2773722627737, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1786951, Length=135, Percent_Identity=27.4074074074074, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF00381 PTS-HPr; PF00359 PTS_EIIA_2 [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 94869; Mature: 94738

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00369 PTS_HPR_HIS ; PS51094 PTS_EIIA_TYPE_2 ; PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKQVTFICGLLNGVHARPASHIERVCNRFQSRFHWYNPRSGIVGDGKSVLSLIAANILL
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHEE
GDECQVTIEGEDEQVAFERLSIFIEHELPHADPILPKREESAEWEPLPASLAHLHPTLLR
CCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ARSVSPGTACGKLLSLIRADLNALGDLPVAQGIEREQQMLADGVAQLGKAWESLLVANSS
HCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
TAANSSTTENNSTTENNSTTRAIREVHRSLLRDGTFRQRLLSHIVAGESCATAIVATAAY
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
FSQQLALAANTYLRERELDIRDVSFQLLQQIYGEQRFPSQQALSEDSLCIADELTPSQFL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHCCCCCEEEECCCCHHHHH
ALDKRYLKGLLLGRGGSTSHTVILARSFNIPTLVGVDAAALQPYINQSLQIDGELGLVVC
HHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEECCCHHHHHHHHCCCEEECCCCCEEEE
LLDEPVRRYYRQEQWLHDQLREQQSRYQNMPGRTLDGVRMVVAANITHAVEVEGAFNQGA
ECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEHEECCCEEEEECCCHHCCH
ESIGLFRTEILYMDRAAAPSEEELYTLYAQALGAAKGKPIIIRTIDIGGDKPVSYLNIPA
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEECCC
ESNPFLGYRAVRIYHEFLSLFHTQLRAILRASMHGPLKIMIPMISSMEEILWVKDQLAEV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHH
KQSLRISHLQFDETVPLGMMLEVPSVMFIIDQCCEEMDFLSIGSNDLTQYLLAVDRDNAK
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCC
VSEHYHCLPPALLRALDYAVCEVHRHGKWIGLCGELAAKDSVLPLLVAMGLDEISMSASF
HHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEHHHHHCCCCHHHHHHHHCCHHHHHHHHH
IGATKARLAKLDRGECRLLLNRVMACRTSREVEYLLVQYDGEQRDEPLIIPECITLGADW
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEECHHHHHCCCCC
RSKEEVIKGMVDNLLLAGRCRYPRKLAADVWAREALFSTGLGFGFAIPHAQSEHIEQSTI
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHH
SVAKLAKPVNWGEEDALFVMMLTLNKQASGDQHMRIFSKLARRIMHEGFRDALVSAGTAQ
HHHHHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
QVEMLLKHELEL
HHHHHHHHHCCC
>Mature Secondary Structure 
TKQVTFICGLLNGVHARPASHIERVCNRFQSRFHWYNPRSGIVGDGKSVLSLIAANILL
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHEE
GDECQVTIEGEDEQVAFERLSIFIEHELPHADPILPKREESAEWEPLPASLAHLHPTLLR
CCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ARSVSPGTACGKLLSLIRADLNALGDLPVAQGIEREQQMLADGVAQLGKAWESLLVANSS
HCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
TAANSSTTENNSTTENNSTTRAIREVHRSLLRDGTFRQRLLSHIVAGESCATAIVATAAY
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
FSQQLALAANTYLRERELDIRDVSFQLLQQIYGEQRFPSQQALSEDSLCIADELTPSQFL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHCCCCCEEEECCCCHHHHH
ALDKRYLKGLLLGRGGSTSHTVILARSFNIPTLVGVDAAALQPYINQSLQIDGELGLVVC
HHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEECCCHHHHHHHHCCCEEECCCCCEEEE
LLDEPVRRYYRQEQWLHDQLREQQSRYQNMPGRTLDGVRMVVAANITHAVEVEGAFNQGA
ECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEHEECCCEEEEECCCHHCCH
ESIGLFRTEILYMDRAAAPSEEELYTLYAQALGAAKGKPIIIRTIDIGGDKPVSYLNIPA
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEECCC
ESNPFLGYRAVRIYHEFLSLFHTQLRAILRASMHGPLKIMIPMISSMEEILWVKDQLAEV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHH
KQSLRISHLQFDETVPLGMMLEVPSVMFIIDQCCEEMDFLSIGSNDLTQYLLAVDRDNAK
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCC
VSEHYHCLPPALLRALDYAVCEVHRHGKWIGLCGELAAKDSVLPLLVAMGLDEISMSASF
HHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEHHHHHCCCCHHHHHHHHCCHHHHHHHHH
IGATKARLAKLDRGECRLLLNRVMACRTSREVEYLLVQYDGEQRDEPLIIPECITLGADW
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEECHHHHHCCCCC
RSKEEVIKGMVDNLLLAGRCRYPRKLAADVWAREALFSTGLGFGFAIPHAQSEHIEQSTI
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHH
SVAKLAKPVNWGEEDALFVMMLTLNKQASGDQHMRIFSKLARRIMHEGFRDALVSAGTAQ
HHHHHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
QVEMLLKHELEL
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]