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The map label for this gene is dusA

Identifier: 218927522

GI number: 218927522

Start: 323066

End: 324103

Strand: Direct

Name: dusA

Synonym: YPO0316

Alternate gene names: 218927522

Gene position: 323066-324103 (Clockwise)

Preceding gene: 218927520

Following gene: 218927523

Centisome position: 6.94

GC content: 51.93

Gene sequence:

>1038_bases
ATGCACGAAGCTCAGACTTTTTCCAGTACGCCGGCCACCAAGCCGCAATACCCCCTGCAACGCTTCTCCGTCGCGCCGAT
GCTCGATTGGACGGATCGTCATTGCCGTTATTTTCACCGTCTGCTCACTAAGCAAGCTCTGTTGTATACCGAAATGGTGA
CCACCGGCGCGATTATCCACGGTAAAGCCGATTATCTGGCTTATAGCGAGCAGGACCATCCGGTCGCTTTGCAATTAGGG
GGCAGCGATCCACAGGCATTGGCGCACTGTGCCAAGCTGGCTGAGCAACGCGGTTATAACGAAATCAATCTGAATGTGGG
TTGTCCGTCAGATCGAGTACAAAATGGCCGCTTTGGTGCTTGTTTGATGGGGGAGGCCGATCTGGTTGCCGATTGCATTA
AAGCTATGCGTGATGCGGTAGCTATTCCGGTGACGGTAAAAACCCGTATTGGGATTGACCAACTGGATAGCTATGAGTTT
CTATGCGAATTCGTGCAAACCGTCGCAGAACGTGGCGAATGCGAAATTTTCACTATTCATGCCCGTAAAGCCTGGCTCTC
AGGGCTAAGCCCGAAAGAGAATCGTGAAGTGCCGCCACTGGATTATGAGCGTGTTTATCAGCTTAAGCGTGATTTCCCTG
CGTTGACGATTGCCATTAATGGTGGGGTGAAAACATTGGCTGAAGCTAAAGAGCACCTCAAGCACCTTGATGGCGTGATG
ATGGGCCGTGAAGCCTATCAGAATCCGGGAATTTTGACGCAAGTAGACCGCGAGCTGTTTGATCCTAACGCGCCAGTGGT
TGATAGCGTTAAAGCCATTGAGGCGCTGTATCCTTATATTGAGCAGGAATTGTCTCAAGGGGCCTATTTGGGCCACATAA
CCCGCCATATTCTGGGTATTTTCCAAGGAATCCCCGGAGCGCGCCAATGGCGTCGGCATTTAAGCGAAAATGCCCATAAG
CCCGGAGCGGGGGTTTCTGTTGTGGAGGAGGCTTTAGCGCTGGTATCCCCTTCGTACTACGAATCCGTAGGGGGTTAG

Upstream 100 bases:

>100_bases
TTCTATGGTATAATCAACGGTTCCACCCCTTTGTATCGTTCGCTCAGTGAGCAACATCTGGGGGAAATTAATGCAGTAAT
AACTGATGAAATCACCGTAA

Downstream 100 bases:

>100_bases
CGGCGTTCGTTACTTGTCCTCTTCTACAAATAATTATTATACACATATGGGCTTCACCTCTGCGAGGCCGCTGTCAGCCG
TATTCAAATATGCTTTCGGC

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MHEAQTFSSTPATKPQYPLQRFSVAPMLDWTDRHCRYFHRLLTKQALLYTEMVTTGAIIHGKADYLAYSEQDHPVALQLG
GSDPQALAHCAKLAEQRGYNEINLNVGCPSDRVQNGRFGACLMGEADLVADCIKAMRDAVAIPVTVKTRIGIDQLDSYEF
LCEFVQTVAERGECEIFTIHARKAWLSGLSPKENREVPPLDYERVYQLKRDFPALTIAINGGVKTLAEAKEHLKHLDGVM
MGREAYQNPGILTQVDRELFDPNAPVVDSVKAIEALYPYIEQELSQGAYLGHITRHILGIFQGIPGARQWRRHLSENAHK
PGAGVSVVEEALALVSPSYYESVGG

Sequences:

>Translated_345_residues
MHEAQTFSSTPATKPQYPLQRFSVAPMLDWTDRHCRYFHRLLTKQALLYTEMVTTGAIIHGKADYLAYSEQDHPVALQLG
GSDPQALAHCAKLAEQRGYNEINLNVGCPSDRVQNGRFGACLMGEADLVADCIKAMRDAVAIPVTVKTRIGIDQLDSYEF
LCEFVQTVAERGECEIFTIHARKAWLSGLSPKENREVPPLDYERVYQLKRDFPALTIAINGGVKTLAEAKEHLKHLDGVM
MGREAYQNPGILTQVDRELFDPNAPVVDSVKAIEALYPYIEQELSQGAYLGHITRHILGIFQGIPGARQWRRHLSENAHK
PGAGVSVVEEALALVSPSYYESVGG
>Mature_345_residues
MHEAQTFSSTPATKPQYPLQRFSVAPMLDWTDRHCRYFHRLLTKQALLYTEMVTTGAIIHGKADYLAYSEQDHPVALQLG
GSDPQALAHCAKLAEQRGYNEINLNVGCPSDRVQNGRFGACLMGEADLVADCIKAMRDAVAIPVTVKTRIGIDQLDSYEF
LCEFVQTVAERGECEIFTIHARKAWLSGLSPKENREVPPLDYERVYQLKRDFPALTIAINGGVKTLAEAKEHLKHLDGVM
MGREAYQNPGILTQVDRELFDPNAPVVDSVKAIEALYPYIEQELSQGAYLGHITRHILGIFQGIPGARQWRRHLSENAHK
PGAGVSVVEEALALVSPSYYESVGG

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily

Homologues:

Organism=Homo sapiens, GI40807366, Length=296, Percent_Identity=23.9864864864865, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI31742496, Length=239, Percent_Identity=28.0334728033473, Blast_Score=75, Evalue=8e-14,
Organism=Escherichia coli, GI145693211, Length=316, Percent_Identity=78.1645569620253, Blast_Score=536, Evalue=1e-154,
Organism=Escherichia coli, GI1789660, Length=246, Percent_Identity=27.6422764227642, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788462, Length=225, Percent_Identity=29.3333333333333, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17507177, Length=168, Percent_Identity=26.1904761904762, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=268, Percent_Identity=25, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24580595, Length=245, Percent_Identity=24.4897959183673, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19920448, Length=245, Percent_Identity=24.4897959183673, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUSA_YERPE (Q8ZJ14)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AG0039
- RefSeq:   NP_667911.1
- RefSeq:   NP_991865.1
- RefSeq:   YP_002345397.1
- ProteinModelPortal:   Q8ZJ14
- SMR:   Q8ZJ14
- GeneID:   1145521
- GeneID:   1173161
- GeneID:   2766140
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO0316
- KEGG:   ypk:y0574
- KEGG:   ypm:YP_0472
- HOGENOM:   HBG630545
- OMA:   RIGIDEQ
- ProtClustDB:   PRK11815
- BioCyc:   YPES187410:Y0574-MONOMER
- BioCyc:   YPES214092:YPO0316-MONOMER
- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR11082
- PIRSF:   PIRSF006621
- TIGRFAMs:   TIGR00742

Pfam domain/function: PF01207 Dus

EC number: 1.-.-.-

Molecular weight: Translated: 38333; Mature: 38333

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHEAQTFSSTPATKPQYPLQRFSVAPMLDWTDRHCRYFHRLLTKQALLYTEMVTTGAIIH
CCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEE
GKADYLAYSEQDHPVALQLGGSDPQALAHCAKLAEQRGYNEINLNVGCPSDRVQNGRFGA
CCHHHEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCE
CLMGEADLVADCIKAMRDAVAIPVTVKTRIGIDQLDSYEFLCEFVQTVAERGECEIFTIH
EECCCHHHHHHHHHHHHHHEECCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
ARKAWLSGLSPKENREVPPLDYERVYQLKRDFPALTIAINGGVKTLAEAKEHLKHLDGVM
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHH
MGREAYQNPGILTQVDRELFDPNAPVVDSVKAIEALYPYIEQELSQGAYLGHITRHILGI
HHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
FQGIPGARQWRRHLSENAHKPGAGVSVVEEALALVSPSYYESVGG
HHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure
MHEAQTFSSTPATKPQYPLQRFSVAPMLDWTDRHCRYFHRLLTKQALLYTEMVTTGAIIH
CCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEE
GKADYLAYSEQDHPVALQLGGSDPQALAHCAKLAEQRGYNEINLNVGCPSDRVQNGRFGA
CCHHHEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCE
CLMGEADLVADCIKAMRDAVAIPVTVKTRIGIDQLDSYEFLCEFVQTVAERGECEIFTIH
EECCCHHHHHHHHHHHHHHEECCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
ARKAWLSGLSPKENREVPPLDYERVYQLKRDFPALTIAINGGVKTLAEAKEHLKHLDGVM
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHH
MGREAYQNPGILTQVDRELFDPNAPVVDSVKAIEALYPYIEQELSQGAYLGHITRHILGI
HHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
FQGIPGARQWRRHLSENAHKPGAGVSVVEEALALVSPSYYESVGG
HHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430