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The map label for this gene is sigF [H]

Identifier: 218905210

GI number: 218905210

Start: 3915657

End: 3916415

Strand: Reverse

Name: sigF [H]

Synonym: BCAH820_4094

Alternate gene names: 218905210

Gene position: 3916415-3915657 (Counterclockwise)

Preceding gene: 218905211

Following gene: 218905209

Centisome position: 73.86

GC content: 36.76

Gene sequence:

>759_bases
ATGGACATAGAGGTCAAAAATGAGAAGAAGAAACCTCAGTTAAAGGACCACGAGCTAAAAGCGTTAATTCAAAAAAGTCA
AGATGGAGATCAACAAGCGAGAGATACAATCGTTCAAAGTAATATGCGTCTCGTATGGTCGGTTGTACAGCGTTTTCTTA
ACCGCGGATACGAACCAGACGATTTATTTCAAATTGGATGTATTGGGCTCTTAAAATCGGTAGATAAATTTGATTTATCT
TTCGACGTGAAATTTTCAACATATGCAGTTCCAATGATTATTGGCGAAATACAGCGATTTTTACGTGATGATGGATCAGT
GAAAGTAAGTAGGTCTTTAAAGGAAACAGGAAACAAAATTCGAAAGATGAGAGACGAGCTTTCGAAAGAATTTGGAAGGG
CTCCAACGATTAATGAAGTAGCTGAAGCGTTAGAACTAACGCCAGAGGAAGTGGTTCTGGCGCAAGAAGCGAGTCGTGCT
CCTTCATCCATACATGAAACGGTGTATGAAAACGACGGAGATCCAATTACTATTTTAGATCAAATTGCAGATCAATCTGA
AACGAAATGGTTTGATAAAATTGCTTTAAAAGAAGCAATTAGAGAATTAGATGAGCGAGAACGTTTAATTGTATATTTAC
GCTACTATAAAGATCAAACGCAATCAGAAGTAGCGGAACGAATAGGTATTTCTCAAGTGCAAGTTTCAAGACTTGAAAAG
AAAATATTAAAACAGATGAAAGATCGAATAGATGAATAG

Upstream 100 bases:

>100_bases
TGAAGTAGAAGTTATTTCAAACGAATCTTTCGGGACAACAATCCATTTGACAAAATACTTATCAAATAGTAACGCTCTAT
GCAATTAAGGAGAATAGCCT

Downstream 100 bases:

>100_bases
TTATCTATTCGATCTTTTTTTATGAATGTAATAGGAGGGGATTATAAACTTTCGTAAAAATTAACAGAATTTATTGATAA
TTTAGTAAATTTAAAATATA

Product: sporulation sigma factor SigF

Products: NA

Alternate protein names: Sporulation sigma factor; Stage II sporulation protein AC [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MDIEVKNEKKKPQLKDHELKALIQKSQDGDQQARDTIVQSNMRLVWSVVQRFLNRGYEPDDLFQIGCIGLLKSVDKFDLS
FDVKFSTYAVPMIIGEIQRFLRDDGSVKVSRSLKETGNKIRKMRDELSKEFGRAPTINEVAEALELTPEEVVLAQEASRA
PSSIHETVYENDGDPITILDQIADQSETKWFDKIALKEAIRELDERERLIVYLRYYKDQTQSEVAERIGISQVQVSRLEK
KILKQMKDRIDE

Sequences:

>Translated_252_residues
MDIEVKNEKKKPQLKDHELKALIQKSQDGDQQARDTIVQSNMRLVWSVVQRFLNRGYEPDDLFQIGCIGLLKSVDKFDLS
FDVKFSTYAVPMIIGEIQRFLRDDGSVKVSRSLKETGNKIRKMRDELSKEFGRAPTINEVAEALELTPEEVVLAQEASRA
PSSIHETVYENDGDPITILDQIADQSETKWFDKIALKEAIRELDERERLIVYLRYYKDQTQSEVAERIGISQVQVSRLEK
KILKQMKDRIDE
>Mature_252_residues
MDIEVKNEKKKPQLKDHELKALIQKSQDGDQQARDTIVQSNMRLVWSVVQRFLNRGYEPDDLFQIGCIGLLKSVDKFDLS
FDVKFSTYAVPMIIGEIQRFLRDDGSVKVSRSLKETGNKIRKMRDELSKEFGRAPTINEVAEALELTPEEVVLAQEASRA
PSSIHETVYENDGDPITILDQIADQSETKWFDKIALKEAIRELDERERLIVYLRYYKDQTQSEVAERIGISQVQVSRLEK
KILKQMKDRIDE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes. It is responsible for directing gene

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=228, Percent_Identity=28.5087719298246, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1789098, Length=250, Percent_Identity=26.4, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789871, Length=273, Percent_Identity=27.4725274725275, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788231, Length=201, Percent_Identity=28.8557213930348, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014236
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 29178; Mature: 29178

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIEVKNEKKKPQLKDHELKALIQKSQDGDQQARDTIVQSNMRLVWSVVQRFLNRGYEPD
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
DLFQIGCIGLLKSVDKFDLSFDVKFSTYAVPMIIGEIQRFLRDDGSVKVSRSLKETGNKI
HHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RKMRDELSKEFGRAPTINEVAEALELTPEEVVLAQEASRAPSSIHETVYENDGDPITILD
HHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHH
QIADQSETKWFDKIALKEAIRELDERERLIVYLRYYKDQTQSEVAERIGISQVQVSRLEK
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHH
KILKQMKDRIDE
HHHHHHHHHHCC
>Mature Secondary Structure
MDIEVKNEKKKPQLKDHELKALIQKSQDGDQQARDTIVQSNMRLVWSVVQRFLNRGYEPD
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
DLFQIGCIGLLKSVDKFDLSFDVKFSTYAVPMIIGEIQRFLRDDGSVKVSRSLKETGNKI
HHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RKMRDELSKEFGRAPTINEVAEALELTPEEVVLAQEASRAPSSIHETVYENDGDPITILD
HHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHH
QIADQSETKWFDKIALKEAIRELDERERLIVYLRYYKDQTQSEVAERIGISQVQVSRLEK
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHH
KILKQMKDRIDE
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1391049 [H]