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The map label for this gene is murD

Identifier: 218905043

GI number: 218905043

Start: 3765409

End: 3766761

Strand: Reverse

Name: murD

Synonym: BCAH820_3927

Alternate gene names: 218905043

Gene position: 3766761-3765409 (Counterclockwise)

Preceding gene: 218905044

Following gene: 218905042

Centisome position: 71.04

GC content: 35.25

Gene sequence:

>1353_bases
TTGAAAACTGTAACTGAATTTCAAAATAAAAATATTCTTGTATTAGGCATTGCAAAAAGTGGTTATGCAGCAGCTACGCT
GTTACAAAAGTTAGGGGCAAATGTTATTGTAAATGACGGAAAGCCGTTAGCAGAAAATGTACTCGCTGCAGAATTACAAG
CAAAAGGAATGGACGTTGTATGCGGTGGTCATCCTTTAGAATTATTAGAGAGAAATATTTCTCTTGTAGTAAAAAATCCA
GGAATCCCGTATTCTAATCCAATATTAGTTGCTGCGAAAGAAAAGCAAATTCCAATTGTTACAGAAGTTGAATTAGCATA
TCGTATTTCAGAAGCACCATTTGTTGGGATTACGGGATCTAACGGTAAAACGACGACGACAATGCTTACATTTGAAATGC
TAAAAGAAGGACAGAAGCATCCGGTAATTGCAGGGAACATTGGAACTGTAGCGTGTGAAGTTGCACAGGATGCAAAAGAA
AATGAAGTTGTAGTTACAGAACTTTCATCGTTCCAATTGATGGGAGTAGAATTGTTCCAACCTAAAATTGCTGCGTTTTT
AAATTTATTTGAGGCCCACTTAGATTATCATGGGACGAAGAAAGAATATGGTTTAGCAAAAGCAAATATTTTTAAAAACC
AAACAGAAAATGATTATAGTGTAATAAATGCAGATGATGCAGATGTGATGGCTTTATCTGCTTATAGTAAAGGTCAAAAA
GTACTATTCTCAACAACGAAAGAAATTGAAGATGGTGCGTGTATAAAAGATAATGCTCTTTATTTTAAAGCTGAAAAAGT
TGTTGAAGTAGACGATATCGTTTTACCTGGACAACATAATTTAGAAAATATTTTAGCAGCGATGAGTATCGCAAAATTAT
TGGGTGTTTCTAACGAAGCGATTACGGCGGTGTTAAAACGTTTTACAGGTGTAAAACATCGTTTAGAATATGTAACAACT
ATAAACAACCGTAAGTTTTATAATGACTCAAAAGCAACGAATATGTTAGCGACAGAGAAAGCTTTATCTGCGTTTACACA
ACCGACTGTGTTATTAGCAGGTGGACTTGATCGTGGGAATGAGTTCGATGATTTAATTCCGTATTTCAAAAATGTTAAAG
CGATTGTAACATTTGGACAAACCGCTCCAAAATTAGTAAGAGCCGCAGAAAAAGCGGGACTAGATACAATTGAAAGTGTC
GATACTTTAGATGAAGCGGTAGTGAAAGCTTATGCTCATTCTACAGATGGCGATGTAATTCTTCTTTCTCCAGCATGTGC
AAGCTGGGATCAATTTAAAACATTTGAAGAAAGAGGAGACATTTTTATACAAGCTGTGCATAAACTTATATAA

Upstream 100 bases:

>100_bases
TGAATTATGTGGTTGGTCAGAGTGGCGTGTTGTTGTAACGTTTTGGTCTGTAGGATTTTTATTAGCTGTGTTAGGAATTT
ATATCGGGGTGTGGATGTAA

Downstream 100 bases:

>100_bases
AGTAAATCAAAACATCAGTAGGCTAACACCTACTGATGTTTTGTTACATAGGGCAAAAGTTTCTGCCTAAAGAGGTGGTG
TTTATGGGTAATGAAGAAAA

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MKTVTEFQNKNILVLGIAKSGYAAATLLQKLGANVIVNDGKPLAENVLAAELQAKGMDVVCGGHPLELLERNISLVVKNP
GIPYSNPILVAAKEKQIPIVTEVELAYRISEAPFVGITGSNGKTTTTMLTFEMLKEGQKHPVIAGNIGTVACEVAQDAKE
NEVVVTELSSFQLMGVELFQPKIAAFLNLFEAHLDYHGTKKEYGLAKANIFKNQTENDYSVINADDADVMALSAYSKGQK
VLFSTTKEIEDGACIKDNALYFKAEKVVEVDDIVLPGQHNLENILAAMSIAKLLGVSNEAITAVLKRFTGVKHRLEYVTT
INNRKFYNDSKATNMLATEKALSAFTQPTVLLAGGLDRGNEFDDLIPYFKNVKAIVTFGQTAPKLVRAAEKAGLDTIESV
DTLDEAVVKAYAHSTDGDVILLSPACASWDQFKTFEERGDIFIQAVHKLI

Sequences:

>Translated_450_residues
MKTVTEFQNKNILVLGIAKSGYAAATLLQKLGANVIVNDGKPLAENVLAAELQAKGMDVVCGGHPLELLERNISLVVKNP
GIPYSNPILVAAKEKQIPIVTEVELAYRISEAPFVGITGSNGKTTTTMLTFEMLKEGQKHPVIAGNIGTVACEVAQDAKE
NEVVVTELSSFQLMGVELFQPKIAAFLNLFEAHLDYHGTKKEYGLAKANIFKNQTENDYSVINADDADVMALSAYSKGQK
VLFSTTKEIEDGACIKDNALYFKAEKVVEVDDIVLPGQHNLENILAAMSIAKLLGVSNEAITAVLKRFTGVKHRLEYVTT
INNRKFYNDSKATNMLATEKALSAFTQPTVLLAGGLDRGNEFDDLIPYFKNVKAIVTFGQTAPKLVRAAEKAGLDTIESV
DTLDEAVVKAYAHSTDGDVILLSPACASWDQFKTFEERGDIFIQAVHKLI
>Mature_450_residues
MKTVTEFQNKNILVLGIAKSGYAAATLLQKLGANVIVNDGKPLAENVLAAELQAKGMDVVCGGHPLELLERNISLVVKNP
GIPYSNPILVAAKEKQIPIVTEVELAYRISEAPFVGITGSNGKTTTTMLTFEMLKEGQKHPVIAGNIGTVACEVAQDAKE
NEVVVTELSSFQLMGVELFQPKIAAFLNLFEAHLDYHGTKKEYGLAKANIFKNQTENDYSVINADDADVMALSAYSKGQK
VLFSTTKEIEDGACIKDNALYFKAEKVVEVDDIVLPGQHNLENILAAMSIAKLLGVSNEAITAVLKRFTGVKHRLEYVTT
INNRKFYNDSKATNMLATEKALSAFTQPTVLLAGGLDRGNEFDDLIPYFKNVKAIVTFGQTAPKLVRAAEKAGLDTIESV
DTLDEAVVKAYAHSTDGDVILLSPACASWDQFKTFEERGDIFIQAVHKLI

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=462, Percent_Identity=33.1168831168831, Blast_Score=190, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_BACAA (C3P682)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002868148.1
- ProteinModelPortal:   C3P682
- SMR:   C3P682
- EnsemblBacteria:   EBBACT00000128568
- GeneID:   7849992
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_4077
- GeneTree:   EBGT00070000032130
- ProtClustDB:   PRK02472
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 48902; Mature: 48902

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTVTEFQNKNILVLGIAKSGYAAATLLQKLGANVIVNDGKPLAENVLAAELQAKGMDVV
CCCCHHHCCCCEEEEEECCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCEEE
CGGHPLELLERNISLVVKNPGIPYSNPILVAAKEKQIPIVTEVELAYRISEAPFVGITGS
ECCCHHHHHCCCCEEEEECCCCCCCCCEEEEECCCCCCEEEEEHEEEEECCCCEEEEECC
NGKTTTTMLTFEMLKEGQKHPVIAGNIGTVACEVAQDAKENEVVVTELSSFQLMGVELFQ
CCCEEEHEEEHHHHHCCCCCCEEECCCCCEEEEHHHCCCCCCEEEEECCCEEEECHHHHH
PKIAAFLNLFEAHLDYHGTKKEYGLAKANIFKNQTENDYSVINADDADVMALSAYSKGQK
HHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHCCCCCCCCEEEECCCCCEEEEECCCCCCE
VLFSTTKEIEDGACIKDNALYFKAEKVVEVDDIVLPGQHNLENILAAMSIAKLLGVSNEA
EEEEEHHCCCCCCEEECCEEEEEECCEEEECCEEECCCCCHHHHHHHHHHHHHHCCCHHH
ITAVLKRFTGVKHRLEYVTTINNRKFYNDSKATNMLATEKALSAFTQPTVLLAGGLDRGN
HHHHHHHHHHHHHHHHHEEEECCCEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCC
EFDDLIPYFKNVKAIVTFGQTAPKLVRAAEKAGLDTIESVDTLDEAVVKAYAHSTDGDVI
CHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEE
LLSPACASWDQFKTFEERGDIFIQAVHKLI
EECCCCCCHHHHHHHHHHCHHHHHHHHHHC
>Mature Secondary Structure
MKTVTEFQNKNILVLGIAKSGYAAATLLQKLGANVIVNDGKPLAENVLAAELQAKGMDVV
CCCCHHHCCCCEEEEEECCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCEEE
CGGHPLELLERNISLVVKNPGIPYSNPILVAAKEKQIPIVTEVELAYRISEAPFVGITGS
ECCCHHHHHCCCCEEEEECCCCCCCCCEEEEECCCCCCEEEEEHEEEEECCCCEEEEECC
NGKTTTTMLTFEMLKEGQKHPVIAGNIGTVACEVAQDAKENEVVVTELSSFQLMGVELFQ
CCCEEEHEEEHHHHHCCCCCCEEECCCCCEEEEHHHCCCCCCEEEEECCCEEEECHHHHH
PKIAAFLNLFEAHLDYHGTKKEYGLAKANIFKNQTENDYSVINADDADVMALSAYSKGQK
HHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHCCCCCCCCEEEECCCCCEEEEECCCCCCE
VLFSTTKEIEDGACIKDNALYFKAEKVVEVDDIVLPGQHNLENILAAMSIAKLLGVSNEA
EEEEEHHCCCCCCEEECCEEEEEECCEEEECCEEECCCCCHHHHHHHHHHHHHHCCCHHH
ITAVLKRFTGVKHRLEYVTTINNRKFYNDSKATNMLATEKALSAFTQPTVLLAGGLDRGN
HHHHHHHHHHHHHHHHHEEEECCCEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCC
EFDDLIPYFKNVKAIVTFGQTAPKLVRAAEKAGLDTIESVDTLDEAVVKAYAHSTDGDVI
CHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEE
LLSPACASWDQFKTFEERGDIFIQAVHKLI
EECCCCCCHHHHHHHHHHCHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA