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The map label for this gene is carA

Identifier: 218905017

GI number: 218905017

Start: 3740488

End: 3741585

Strand: Reverse

Name: carA

Synonym: BCAH820_3901

Alternate gene names: 218905017

Gene position: 3741585-3740488 (Counterclockwise)

Preceding gene: 218905018

Following gene: 218905016

Centisome position: 70.56

GC content: 37.52

Gene sequence:

>1098_bases
ATGAAAAGACAACTTATCTTAGAAGATGGAACAGTATTAATTGGAACAGGTTTCGGAGGAGAAATCGAAAAGTCAGGTGA
GGTTGTATTTACAACAGGAATGACTGGATATCAAGAAACATTATCTGATCCATCATATTGCGGTCAAATTGTAACATTCA
CGTACCCACTAATCGGAAACTACGGCATCAACCGTGATGATTTTGAATCGATTCACCCATCTGTAAATGGTTTAATCGTA
AATGAAATTTGTGATCACCCATCAAATTTCCGTAACGAAATTTCGTTAAATGACTACTTAAAAGAAAGAAACATCCCAGG
ATTAGCAGGGATTGATACGAGAAAATTAACGAGAAAAATTCGTCAATACGGTACATTACGCGGACGCCTATGTAACATGG
ATGCAGATGTAGAGTACATCGTGAGTCAATTAAAAGCGACAGTATTTACAGATCATGTAAAACGCGTATCAACGAAAGAT
CCATATCCAAGCCCAGGCCGCGGACATCGCGTCGTACTAGTAGACTTCGGTATGAAACATGGTATTTTACGAGAATTAAA
TAAACGTGATTGTGATGTAATTGTAGTTCCTTACAACACAACAGCCGAAGAAATTTTACGCCTTAGTCCAGATGGAATTA
TGTTAAGTAACGGACCTGGGGATCCAAAAGATGTACCAGAAGCAATTGAAATGTTAAAAGACATTATCGGTAAAGTTCCT
TTATTCGGAATTTGCCTAGGACATCAATTGTTCGCTCTAGCATCTGGTGCGAATACAAGTAAGTTAAAATTCGGTCACCG
TGGTTTAAACCATCCAGTAAAAAATATTGCAACTGGAAAAGTAGCAATTACATCTCAAAACCATGGTTACGCAGTAGAAG
AAGAATCAGTTGAAAATACAGAGCTTGAAATTACACATGTTGCTTTAAATGATGGAACAGTAGAAGGTCTTCGTCATAAG
AAGTTTCCAGCATTTACAGTTCAATACCATCCAGAAGCTTCAGCAGGACCAGAAGATGCAAATGATTTATTCGAAGATTT
CTTAACAATGATTGAAAACTTCAAGAAAGAAGGGGAAGAGTTATGCCAAAACGCCTAG

Upstream 100 bases:

>100_bases
AAGGAAAAAATACACCATTCGCAGGTTGGAAATGCCAAGGATGGCCGGTAATGACAATCGTTGGTGGTAAGATCGCATGG
CAAAAGGAGAGTGCATTAGT

Downstream 100 bases:

>100_bases
ACATTAACACAATTTTAGTAATCGGATCAGGACCAATTGTAATTGGGCAAGCAGCGGAGTTTGACTACTCTGGTACACAA
GCTTGTCAATCTCTTAAAGA

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MKRQLILEDGTVLIGTGFGGEIEKSGEVVFTTGMTGYQETLSDPSYCGQIVTFTYPLIGNYGINRDDFESIHPSVNGLIV
NEICDHPSNFRNEISLNDYLKERNIPGLAGIDTRKLTRKIRQYGTLRGRLCNMDADVEYIVSQLKATVFTDHVKRVSTKD
PYPSPGRGHRVVLVDFGMKHGILRELNKRDCDVIVVPYNTTAEEILRLSPDGIMLSNGPGDPKDVPEAIEMLKDIIGKVP
LFGICLGHQLFALASGANTSKLKFGHRGLNHPVKNIATGKVAITSQNHGYAVEEESVENTELEITHVALNDGTVEGLRHK
KFPAFTVQYHPEASAGPEDANDLFEDFLTMIENFKKEGEELCQNA

Sequences:

>Translated_365_residues
MKRQLILEDGTVLIGTGFGGEIEKSGEVVFTTGMTGYQETLSDPSYCGQIVTFTYPLIGNYGINRDDFESIHPSVNGLIV
NEICDHPSNFRNEISLNDYLKERNIPGLAGIDTRKLTRKIRQYGTLRGRLCNMDADVEYIVSQLKATVFTDHVKRVSTKD
PYPSPGRGHRVVLVDFGMKHGILRELNKRDCDVIVVPYNTTAEEILRLSPDGIMLSNGPGDPKDVPEAIEMLKDIIGKVP
LFGICLGHQLFALASGANTSKLKFGHRGLNHPVKNIATGKVAITSQNHGYAVEEESVENTELEITHVALNDGTVEGLRHK
KFPAFTVQYHPEASAGPEDANDLFEDFLTMIENFKKEGEELCQNA
>Mature_365_residues
MKRQLILEDGTVLIGTGFGGEIEKSGEVVFTTGMTGYQETLSDPSYCGQIVTFTYPLIGNYGINRDDFESIHPSVNGLIV
NEICDHPSNFRNEISLNDYLKERNIPGLAGIDTRKLTRKIRQYGTLRGRLCNMDADVEYIVSQLKATVFTDHVKRVSTKD
PYPSPGRGHRVVLVDFGMKHGILRELNKRDCDVIVVPYNTTAEEILRLSPDGIMLSNGPGDPKDVPEAIEMLKDIIGKVP
LFGICLGHQLFALASGANTSKLKFGHRGLNHPVKNIATGKVAITSQNHGYAVEEESVENTELEITHVALNDGTVEGLRHK
KFPAFTVQYHPEASAGPEDANDLFEDFLTMIENFKKEGEELCQNA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI18105007, Length=366, Percent_Identity=41.5300546448087, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI21361331, Length=367, Percent_Identity=36.2397820163488, Blast_Score=215, Evalue=6e-56,
Organism=Homo sapiens, GI169790915, Length=367, Percent_Identity=36.2397820163488, Blast_Score=215, Evalue=6e-56,
Organism=Escherichia coli, GI1786215, Length=379, Percent_Identity=45.646437994723, Blast_Score=306, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI193204318, Length=371, Percent_Identity=40.1617250673854, Blast_Score=254, Evalue=6e-68,
Organism=Saccharomyces cerevisiae, GI6324878, Length=377, Percent_Identity=39.2572944297082, Blast_Score=262, Evalue=7e-71,
Organism=Saccharomyces cerevisiae, GI6322331, Length=392, Percent_Identity=37.7551020408163, Blast_Score=255, Evalue=9e-69,
Organism=Saccharomyces cerevisiae, GI6322638, Length=152, Percent_Identity=34.2105263157895, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI45555749, Length=377, Percent_Identity=40.3183023872679, Blast_Score=258, Evalue=6e-69,
Organism=Drosophila melanogaster, GI24642586, Length=377, Percent_Identity=40.3183023872679, Blast_Score=257, Evalue=1e-68,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARA_BACAN (Q81WF1)

Other databases:

- EMBL:   AE016879
- EMBL:   AE017334
- EMBL:   AE017225
- RefSeq:   NP_846267.1
- RefSeq:   YP_020668.1
- RefSeq:   YP_029989.1
- ProteinModelPortal:   Q81WF1
- SMR:   Q81WF1
- EnsemblBacteria:   EBBACT00000008201
- EnsemblBacteria:   EBBACT00000016646
- EnsemblBacteria:   EBBACT00000020118
- GeneID:   1086653
- GeneID:   2819581
- GeneID:   2852140
- GenomeReviews:   AE016879_GR
- GenomeReviews:   AE017225_GR
- GenomeReviews:   AE017334_GR
- KEGG:   ban:BA_4026
- KEGG:   bar:GBAA_4026
- KEGG:   bat:BAS3738
- TIGR:   BA_4026
- TIGR:   GBAA_4026
- GeneTree:   EBGT00070000032080
- HOGENOM:   HBG286341
- OMA:   FTYPELG
- ProtClustDB:   PRK12564
- BioCyc:   BANT260799:BAS3738-MONOMER
- BioCyc:   BANT261594:GBAA4026-MONOMER
- BRENDA:   6.3.5.5
- HAMAP:   MF_01209_B
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- PANTHER:   PTHR11405:SF4
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01368

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase; SSF52021 CP_synthsmall

EC number: =6.3.5.5

Molecular weight: Translated: 40377; Mature: 40377

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 245-245 ACT_SITE 330-330 ACT_SITE 332-332

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRQLILEDGTVLIGTGFGGEIEKSGEVVFTTGMTGYQETLSDPSYCGQIVTFTYPLIGN
CCCEEEEECCCEEEECCCCCCCCCCCCEEEEECCCCHHHHCCCHHHCCEEEEEEEHHHCC
YGINRDDFESIHPSVNGLIVNEICDHPSNFRNEISLNDYLKERNIPGLAGIDTRKLTRKI
CCCCHHHHHHHCCCCCCEEHHHHHCCCCCHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHH
RQYGTLRGRLCNMDADVEYIVSQLKATVFTDHVKRVSTKDPYPSPGRGHRVVLVDFGMKH
HHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCHH
GILRELNKRDCDVIVVPYNTTAEEILRLSPDGIMLSNGPGDPKDVPEAIEMLKDIIGKVP
HHHHHHCCCCCCEEEECCCCCHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCC
LFGICLGHQLFALASGANTSKLKFGHRGLNHPVKNIATGKVAITSQNHGYAVEEESVENT
HHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHCCCCEEEEEECCCCEEECCCCCCCC
ELEITHVALNDGTVEGLRHKKFPAFTVQYHPEASAGPEDANDLFEDFLTMIENFKKEGEE
EEEEEEEEECCCCHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LCQNA
HHCCC
>Mature Secondary Structure
MKRQLILEDGTVLIGTGFGGEIEKSGEVVFTTGMTGYQETLSDPSYCGQIVTFTYPLIGN
CCCEEEEECCCEEEECCCCCCCCCCCCEEEEECCCCHHHHCCCHHHCCEEEEEEEHHHCC
YGINRDDFESIHPSVNGLIVNEICDHPSNFRNEISLNDYLKERNIPGLAGIDTRKLTRKI
CCCCHHHHHHHCCCCCCEEHHHHHCCCCCHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHH
RQYGTLRGRLCNMDADVEYIVSQLKATVFTDHVKRVSTKDPYPSPGRGHRVVLVDFGMKH
HHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCHH
GILRELNKRDCDVIVVPYNTTAEEILRLSPDGIMLSNGPGDPKDVPEAIEMLKDIIGKVP
HHHHHHCCCCCCEEEECCCCCHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCC
LFGICLGHQLFALASGANTSKLKFGHRGLNHPVKNIATGKVAITSQNHGYAVEEESVENT
HHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHCCCCEEEEEECCCCEEECCCCCCCC
ELEITHVALNDGTVEGLRHKKFPAFTVQYHPEASAGPEDANDLFEDFLTMIENFKKEGEE
EEEEEEEEECCCCHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LCQNA
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629