BacMap

Definition	Bacillus cereus AH820, complete genome.
Accession	NC_011773
Length	5,302,683

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The map label for this gene is gmk

Identifier: 218905001

GI number: 218905001

Start: 3722902

End: 3723519

Strand: Reverse

Name: gmk

Synonym: BCAH820_3885

Alternate gene names: 218905001

Gene position: 3723519-3722902 (Counterclockwise)

Preceding gene: 218905002

Following gene: 218905000

Centisome position: 70.22

GC content: 38.35

Gene sequence:

>618_bases
ATGAGAAGTAGAAGAGGATTGCTCATCGTTCTTTCAGGACCTTCTGGTGTTGGGAAAGGAACGGTTCGAAAAGAGCTGTT
TAGTCATGAGGATACACGTTTTCAGTACTCCATTTCAGTAACGACACGTAAGCCACGTGAAGGTGAAGTGGATGGTGTGG
ATTATTTCTTTAAAGAGAGAGAAGAATTTGAGGAAATGATTCGTAATGAAAAATTACTTGAGTGGGCTGAGTTCGTAGGT
AATTATTACGGAACACCAATTGACTATGTTGAAAAAACATTGCAAGAAGGAAAAGATGTATTCTTAGAAATTGAAGTGCA
AGGAGCAATTCAAGTTAAGAAAGCTTTCCCAGAAGGTGTATTTATTTTCTTAGCACCTCCAAGTTTATCTGAACTAAAGA
ACCGAATTGTCGGTCGTGGTACAGAAACTGAAGATGTTATTGAAAATCGTTTAACGGTAGCGAAAGAAGAAATTGATATG
ATGGACGCTTACGACTACGTTGTAGAAAACGACCAAGTTGAACTAGCTTGTGAAAGAATTAAAGCAATTGTGGTTGGCGA
ACATTGCCGCCGCGAAAGAGTAGCAAAATATTATAAAGAAATGACGGAGGGTCTATAA

Upstream 100 bases:

>100_bases
GATGGGCATGTTGTATTAAGTCCAATTCAGCCAGAGACGATTGCACATCGTTTGAATAATAAAGAAGATTTAAGTGAGGA
AGGGTAGGTTTTACATATTT

Downstream 100 bases:

>100_bases
TTATGTTAAATCCATCAATTGATTCATTATTAACAAAAATCGATTCTAAATATACACTTGTAACAGTGGCTGCAAAACGT
GCGCGCGAAATGCAACTTGC

Product: guanylate kinase

Products: NA

Alternate protein names: GMP kinase

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MRSRRGLLIVLSGPSGVGKGTVRKELFSHEDTRFQYSISVTTRKPREGEVDGVDYFFKEREEFEEMIRNEKLLEWAEFVG
NYYGTPIDYVEKTLQEGKDVFLEIEVQGAIQVKKAFPEGVFIFLAPPSLSELKNRIVGRGTETEDVIENRLTVAKEEIDM
MDAYDYVVENDQVELACERIKAIVVGEHCRRERVAKYYKEMTEGL

Sequences:

>Translated_205_residues
MRSRRGLLIVLSGPSGVGKGTVRKELFSHEDTRFQYSISVTTRKPREGEVDGVDYFFKEREEFEEMIRNEKLLEWAEFVG
NYYGTPIDYVEKTLQEGKDVFLEIEVQGAIQVKKAFPEGVFIFLAPPSLSELKNRIVGRGTETEDVIENRLTVAKEEIDM
MDAYDYVVENDQVELACERIKAIVVGEHCRRERVAKYYKEMTEGL
>Mature_205_residues
MRSRRGLLIVLSGPSGVGKGTVRKELFSHEDTRFQYSISVTTRKPREGEVDGVDYFFKEREEFEEMIRNEKLLEWAEFVG
NYYGTPIDYVEKTLQEGKDVFLEIEVQGAIQVKKAFPEGVFIFLAPPSLSELKNRIVGRGTETEDVIENRLTVAKEEIDM
MDAYDYVVENDQVELACERIKAIVVGEHCRRERVAKYYKEMTEGL

Specific function: Essential for recycling GMP and indirectly, cGMP

COG id: COG0194

COG function: function code F; Guanylate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 guanylate kinase-like domain

Homologues:

Organism=Homo sapiens, GI226874831, Length=176, Percent_Identity=36.3636363636364, Blast_Score=112, Evalue=2e-25,
Organism=Homo sapiens, GI226874833, Length=176, Percent_Identity=36.3636363636364, Blast_Score=112, Evalue=2e-25,
Organism=Homo sapiens, GI4504221, Length=176, Percent_Identity=36.3636363636364, Blast_Score=112, Evalue=2e-25,
Organism=Homo sapiens, GI14780902, Length=183, Percent_Identity=30.0546448087432, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI21361598, Length=184, Percent_Identity=30.4347826086957, Blast_Score=84, Evalue=7e-17,
Organism=Homo sapiens, GI38570142, Length=145, Percent_Identity=34.4827586206897, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI4505237, Length=136, Percent_Identity=33.0882352941176, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI262118255, Length=136, Percent_Identity=33.0882352941176, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI262118263, Length=136, Percent_Identity=33.0882352941176, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI262118259, Length=136, Percent_Identity=33.0882352941176, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI186700627, Length=159, Percent_Identity=27.0440251572327, Blast_Score=69, Evalue=2e-12,
Organism=Homo sapiens, GI186700629, Length=159, Percent_Identity=27.0440251572327, Blast_Score=69, Evalue=2e-12,
Organism=Homo sapiens, GI186972120, Length=159, Percent_Identity=27.0440251572327, Blast_Score=69, Evalue=2e-12,
Organism=Homo sapiens, GI111154074, Length=112, Percent_Identity=30.3571428571429, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI119943106, Length=177, Percent_Identity=28.2485875706215, Blast_Score=68, Evalue=5e-12,
Organism=Homo sapiens, GI52630447, Length=152, Percent_Identity=29.6052631578947, Blast_Score=67, Evalue=8e-12,
Organism=Homo sapiens, GI192447426, Length=181, Percent_Identity=27.6243093922652, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI119943108, Length=177, Percent_Identity=28.2485875706215, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI21389483, Length=181, Percent_Identity=24.3093922651934, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI4557529, Length=181, Percent_Identity=27.6243093922652, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI261862237, Length=177, Percent_Identity=28.2485875706215, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1790080, Length=204, Percent_Identity=40.6862745098039, Blast_Score=155, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17508835, Length=172, Percent_Identity=40.1162790697674, Blast_Score=125, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI193203132, Length=172, Percent_Identity=40.1162790697674, Blast_Score=125, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71988048, Length=131, Percent_Identity=32.0610687022901, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI71988041, Length=131, Percent_Identity=32.0610687022901, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17556244, Length=124, Percent_Identity=34.6774193548387, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71984092, Length=118, Percent_Identity=29.6610169491525, Blast_Score=67, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17557440, Length=121, Percent_Identity=29.7520661157025, Blast_Score=67, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6320662, Length=183, Percent_Identity=45.3551912568306, Blast_Score=163, Evalue=2e-41,
Organism=Drosophila melanogaster, GI21356141, Length=182, Percent_Identity=41.2087912087912, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI161077626, Length=176, Percent_Identity=28.9772727272727, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24640542, Length=176, Percent_Identity=28.9772727272727, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI45554489, Length=176, Percent_Identity=28.9772727272727, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI85724806, Length=176, Percent_Identity=28.9772727272727, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI45554481, Length=176, Percent_Identity=28.9772727272727, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI116007136, Length=176, Percent_Identity=28.9772727272727, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI85816281, Length=127, Percent_Identity=33.8582677165354, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI85816106, Length=127, Percent_Identity=33.8582677165354, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI45551002, Length=127, Percent_Identity=33.8582677165354, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI221330166, Length=160, Percent_Identity=30.625, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI221330162, Length=160, Percent_Identity=30.625, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI221330164, Length=154, Percent_Identity=31.8181818181818, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24641280, Length=179, Percent_Identity=26.8156424581006, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45554804, Length=179, Percent_Identity=26.8156424581006, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI281360700, Length=179, Percent_Identity=26.8156424581006, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24641282, Length=179, Percent_Identity=26.8156424581006, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45554818, Length=179, Percent_Identity=26.8156424581006, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI161077752, Length=179, Percent_Identity=26.8156424581006, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45554813, Length=179, Percent_Identity=26.8156424581006, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI45554791, Length=179, Percent_Identity=26.8156424581006, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KGUA_BACAN (Q81WG7)

Other databases:

- EMBL:   AE016879
- EMBL:   AE017334
- EMBL:   AE017225
- RefSeq:   NP_846251.1
- RefSeq:   YP_020651.1
- RefSeq:   YP_029973.1
- ProteinModelPortal:   Q81WG7
- SMR:   Q81WG7
- EnsemblBacteria:   EBBACT00000008358
- EnsemblBacteria:   EBBACT00000018508
- EnsemblBacteria:   EBBACT00000020920
- GeneID:   1087515
- GeneID:   2815222
- GeneID:   2848043
- GenomeReviews:   AE016879_GR
- GenomeReviews:   AE017225_GR
- GenomeReviews:   AE017334_GR
- KEGG:   ban:BA_4009
- KEGG:   bar:GBAA_4009
- KEGG:   bat:BAS3722
- TIGR:   BA_4009
- TIGR:   GBAA_4009
- GeneTree:   EBGT00050000002748
- HOGENOM:   HBG671982
- OMA:   EWAEFAG
- ProtClustDB:   PRK00300
- BioCyc:   BANT260799:BAS3722-MONOMER
- BioCyc:   BANT261594:GBAA4009-MONOMER
- BRENDA:   2.7.4.8
- GO:   GO:0005737
- HAMAP:   MF_00328
- InterPro:   IPR008144
- InterPro:   IPR008145
- InterPro:   IPR020590
- InterPro:   IPR017665
- SMART:   SM00072
- TIGRFAMs:   TIGR03263

Pfam domain/function: PF00625 Guanylate_kin

EC number: =2.7.4.8

Molecular weight: Translated: 23677; Mature: 23677

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00856 GUANYLATE_KINASE_1; PS50052 GUANYLATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSRRGLLIVLSGPSGVGKGTVRKELFSHEDTRFQYSISVTTRKPREGEVDGVDYFFKER
CCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHH
EEFEEMIRNEKLLEWAEFVGNYYGTPIDYVEKTLQEGKDVFLEIEVQGAIQVKKAFPEGV
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEECCCEEEECCCCCCE
FIFLAPPSLSELKNRIVGRGTETEDVIENRLTVAKEEIDMMDAYDYVVENDQVELACERI
EEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KAIVVGEHCRRERVAKYYKEMTEGL
HHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRSRRGLLIVLSGPSGVGKGTVRKELFSHEDTRFQYSISVTTRKPREGEVDGVDYFFKER
CCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHH
EEFEEMIRNEKLLEWAEFVGNYYGTPIDYVEKTLQEGKDVFLEIEVQGAIQVKKAFPEGV
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEECCCEEEECCCCCCE
FIFLAPPSLSELKNRIVGRGTETEDVIENRLTVAKEEIDMMDAYDYVVENDQVELACERI
EEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KAIVVGEHCRRERVAKYYKEMTEGL
HHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629