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The map label for this gene is infB [H]

Identifier: 218904940

GI number: 218904940

Start: 3659686

End: 3660426

Strand: Reverse

Name: infB [H]

Synonym: BCAH820_3824

Alternate gene names: 218904940

Gene position: 3660426-3659686 (Counterclockwise)

Preceding gene: 218904941

Following gene: 218904939

Centisome position: 69.03

GC content: 37.79

Gene sequence:

>741_bases
ATGGCATTCGCTGATGAGAAGAAAGCTCGTCAAATCGGTGAATCACGTGCGCAAGAAGCGTTACTTGCTCAACGTGGTGA
AAAATCTAAATTAAGCCTTGAAGATTTATTCCAACAAATCCAAGAGGGCGATGTAAAAGAAATTAACTTAATTGTGAAAG
CAGACGTACAAGGTTCTGTAGAAGCAATGGCAGCATCACTTCGTAAAATTGATGTTGAAGGTGTTAAAGTTAAAATTATC
CATACAGGCGTAGGTGCGATTACAGAATCTGATATCATTTTAGCTTCTGCATCTAATGCAATTGTAATTGGATTTAACGT
ACGCCCAGATGTGAATGCGAAGCGTACAGCTGAATTAGAGAACGTTGATATTCGTTTACACCGTATTATTTATAAAGTAA
TCGAAGAGATCGAAGCAGCGATGCAAGGTATGTTAGATCCAGAATTCGAAGAAAAAGTAATCGGTCAAGCAGAAGTACGT
CAAACGTTTAAAGTAACAAAAGTTGGAACAATCGCAGGTTGTTACGTAACAGACGGTAAAATTACACGTGATAGTGGCGT
GCGTATTATCCGTGATGGCGTAGTAATTTTCGAAGGACAACTTGATACGTTAAAACGTTTCAAAGACGACGTAAAAGAAG
TTGCACAAAACTATGAGTGTGGTATTACAATTGAGAGATATAATGATCTTAAAGAAGGGGACATTATTGAAGCATACATT
ATGGAAGAAGTGAAGCGATGA

Upstream 100 bases:

>100_bases
TGGTAAGTGATATTGGTCGCCGTGTAAAAGTTGCTGGTCCATCAACTCCTGTTTTGAAATTACAGGTTTAAACGAAGTGC
CACAAGCGGGAGATCGTTTC

Downstream 100 bases:

>100_bases
TAATCGCTTCACTCTCATTCGAGTGTATGATTTACGATGTGCATTCTTTAAAAGAGAAACGAGCAATTTTGCAACGGGTG
CTAACTCGTGTGAAACAGCG

Product: translation initiation factor IF-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MAFADEKKARQIGESRAQEALLAQRGEKSKLSLEDLFQQIQEGDVKEINLIVKADVQGSVEAMAASLRKIDVEGVKVKII
HTGVGAITESDIILASASNAIVIGFNVRPDVNAKRTAELENVDIRLHRIIYKVIEEIEAAMQGMLDPEFEEKVIGQAEVR
QTFKVTKVGTIAGCYVTDGKITRDSGVRIIRDGVVIFEGQLDTLKRFKDDVKEVAQNYECGITIERYNDLKEGDIIEAYI
MEEVKR

Sequences:

>Translated_246_residues
MAFADEKKARQIGESRAQEALLAQRGEKSKLSLEDLFQQIQEGDVKEINLIVKADVQGSVEAMAASLRKIDVEGVKVKII
HTGVGAITESDIILASASNAIVIGFNVRPDVNAKRTAELENVDIRLHRIIYKVIEEIEAAMQGMLDPEFEEKVIGQAEVR
QTFKVTKVGTIAGCYVTDGKITRDSGVRIIRDGVVIFEGQLDTLKRFKDDVKEVAQNYECGITIERYNDLKEGDIIEAYI
MEEVKR
>Mature_245_residues
AFADEKKARQIGESRAQEALLAQRGEKSKLSLEDLFQQIQEGDVKEINLIVKADVQGSVEAMAASLRKIDVEGVKVKIIH
TGVGAITESDIILASASNAIVIGFNVRPDVNAKRTAELENVDIRLHRIIYKVIEEIEAAMQGMLDPEFEEKVIGQAEVRQ
TFKVTKVGTIAGCYVTDGKITRDSGVRIIRDGVVIFEGQLDTLKRFKDDVKEVAQNYECGITIERYNDLKEGDIIEAYIM
EEVKR

Specific function: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 7

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-2 family [H]

Homologues:

Organism=Homo sapiens, GI53729339, Length=204, Percent_Identity=32.3529411764706, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI53729337, Length=204, Percent_Identity=32.3529411764706, Blast_Score=119, Evalue=3e-27,
Organism=Escherichia coli, GI1789559, Length=245, Percent_Identity=51.4285714285714, Blast_Score=256, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI71994658, Length=243, Percent_Identity=28.8065843621399, Blast_Score=91, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6324550, Length=241, Percent_Identity=34.8547717842324, Blast_Score=127, Evalue=2e-30,
Organism=Drosophila melanogaster, GI28572034, Length=212, Percent_Identity=33.9622641509434, Blast_Score=119, Evalue=2e-27,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006847
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000178
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF11987 IF-2; PF04760 IF2_N [H]

EC number: NA

Molecular weight: Translated: 27398; Mature: 27267

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFADEKKARQIGESRAQEALLAQRGEKSKLSLEDLFQQIQEGDVKEINLIVKADVQGSV
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCHH
EAMAASLRKIDVEGVKVKIIHTGVGAITESDIILASASNAIVIGFNVRPDVNAKRTAELE
HHHHHHHHHCCCCCEEEEEEEECCCCEECCCEEEEECCCEEEEEEECCCCCCCHHHCHHH
NVDIRLHRIIYKVIEEIEAAMQGMLDPEFEEKVIGQAEVRQTFKVTKVGTIAGCYVTDGK
CHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHEEECEEEEEEEECCE
ITRDSGVRIIRDGVVIFEGQLDTLKRFKDDVKEVAQNYECGITIERYNDLKEGDIIEAYI
EECCCCCEEEECCEEEEECCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHH
MEEVKR
HHHHCC
>Mature Secondary Structure 
AFADEKKARQIGESRAQEALLAQRGEKSKLSLEDLFQQIQEGDVKEINLIVKADVQGSV
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCHH
EAMAASLRKIDVEGVKVKIIHTGVGAITESDIILASASNAIVIGFNVRPDVNAKRTAELE
HHHHHHHHHCCCCCEEEEEEEECCCCEECCCEEEEECCCEEEEEEECCCCCCCHHHCHHH
NVDIRLHRIIYKVIEEIEAAMQGMLDPEFEEKVIGQAEVRQTFKVTKVGTIAGCYVTDGK
CHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHEEECEEEEEEEECCE
ITRDSGVRIIRDGVVIFEGQLDTLKRFKDDVKEVAQNYECGITIERYNDLKEGDIIEAYI
EECCCCCEEEECCEEEEECCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHH
MEEVKR
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA