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The map label for this gene is alkA [H]

Identifier: 218904869

GI number: 218904869

Start: 3581181

End: 3582092

Strand: Direct

Name: alkA [H]

Synonym: BCAH820_3753

Alternate gene names: 218904869

Gene position: 3581181-3582092 (Clockwise)

Preceding gene: 218904868

Following gene: 218904871

Centisome position: 67.54

GC content: 33.66

Gene sequence:

>912_bases
ATGACAGCCGAATATAGTAACGCATTAATTTTAACAGTTCCAACAGAATTTAGTTTTCAAGAAAATTTACGCTATCTATC
CCGTTCTAACAATGAATGTATGTTCCACATTGAAGATAACAAAATTTATAAAGTTATCCCTGTTCACGATGTAAAGCCTT
TAGTCGAAATTAGTATGAATGCGGATGATACTATTCAAATACGTTTTTTAGGAGAAGCCTATATCTCTGCAAAACCGATA
CGCGATGCTGTAGCTAACTATGTAACTGAATGGTTTGATTTAACTATTGATTTAGCTCCCTTTTATACATTAGCTAAACA
TGATGTACTCCTTCAAAGACCAATTGAACAATATTATGGCCTTCGTACTTTAGGTATTCCAGACTTATTTGAGGCACTTT
CCTGGGGAATTATCGGGCAACAAATTAACCTCACATATGCTTATACACTAAAAAGAAGATTAGTCGAGACATTTGGAAGT
TATGTAGAATGGAACGATAGAAAGCATTGGATATTCCCTTCTCCTGAAACAATTGCCAATTTACACGTAGAGGATCTCAA
AAATTTAAAAATGACGACTAGAAAATGTGAATATTTAATCGGTATTGCAAAGCTTATTACTGAAGGAAATTTATCAAAAG
AATCTTTACTGCAAATACAAGACGTGAAACAAGCTGAAAAAAGATTAACTGCTATACATGGTATAGGACCGTGGACGGCC
AACTATGTTTTAATGCGTTGTTTACGATTTCCTTCTGCTTTTCCAATTGACGATGTTGGTTTGCATAATGCAATTAAATA
TTTAACAGGTTCTGAAAGTAAACCGACTAAACATGAAATAAAAGATTTTGCGGTAAACTGGAAAAATTGGGAGTCTTATG
CGACTTTTTACCTATGGCGAGTATTATACTAA

Upstream 100 bases:

>100_bases
ACCGTGTTATTGGAAAGAATGGCAAACTAACTGGTTTTAGAGGGGGATTAGAAATGAAGAAAGAATTGCTTGTATTAGAA
AAATTACAGGTGGAATTCAT

Downstream 100 bases:

>100_bases
AAAAAACCAAGTAGCACATCTGCACTACTTGGTTCTTTTTTAAGCTTGCTCTTCAAATCGTCTTGCAATCTCTATAATAA
CTTGAACAGCTTTTTCCATA

Product: DNA-3-methyladenine glycosidase

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosidase [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MTAEYSNALILTVPTEFSFQENLRYLSRSNNECMFHIEDNKIYKVIPVHDVKPLVEISMNADDTIQIRFLGEAYISAKPI
RDAVANYVTEWFDLTIDLAPFYTLAKHDVLLQRPIEQYYGLRTLGIPDLFEALSWGIIGQQINLTYAYTLKRRLVETFGS
YVEWNDRKHWIFPSPETIANLHVEDLKNLKMTTRKCEYLIGIAKLITEGNLSKESLLQIQDVKQAEKRLTAIHGIGPWTA
NYVLMRCLRFPSAFPIDDVGLHNAIKYLTGSESKPTKHEIKDFAVNWKNWESYATFYLWRVLY

Sequences:

>Translated_303_residues
MTAEYSNALILTVPTEFSFQENLRYLSRSNNECMFHIEDNKIYKVIPVHDVKPLVEISMNADDTIQIRFLGEAYISAKPI
RDAVANYVTEWFDLTIDLAPFYTLAKHDVLLQRPIEQYYGLRTLGIPDLFEALSWGIIGQQINLTYAYTLKRRLVETFGS
YVEWNDRKHWIFPSPETIANLHVEDLKNLKMTTRKCEYLIGIAKLITEGNLSKESLLQIQDVKQAEKRLTAIHGIGPWTA
NYVLMRCLRFPSAFPIDDVGLHNAIKYLTGSESKPTKHEIKDFAVNWKNWESYATFYLWRVLY
>Mature_302_residues
TAEYSNALILTVPTEFSFQENLRYLSRSNNECMFHIEDNKIYKVIPVHDVKPLVEISMNADDTIQIRFLGEAYISAKPIR
DAVANYVTEWFDLTIDLAPFYTLAKHDVLLQRPIEQYYGLRTLGIPDLFEALSWGIIGQQINLTYAYTLKRRLVETFGSY
VEWNDRKHWIFPSPETIANLHVEDLKNLKMTTRKCEYLIGIAKLITEGNLSKESLLQIQDVKQAEKRLTAIHGIGPWTAN
YVLMRCLRFPSAFPIDDVGLHNAIKYLTGSESKPTKHEIKDFAVNWKNWESYATFYLWRVLY

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2- methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkylbase DNA glycosidase alkA family [H]

Homologues:

Organism=Escherichia coli, GI1788383, Length=181, Percent_Identity=32.5966850828729, Blast_Score=88, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010316
- InterPro:   IPR000035
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR023170
- InterPro:   IPR012904 [H]

Pfam domain/function: PF00730 HhH-GPD; PF07934 OGG_N [H]

EC number: =3.2.2.21 [H]

Molecular weight: Translated: 35256; Mature: 35125

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00516 ALKYLBASE_DNA_GLYCOS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAEYSNALILTVPTEFSFQENLRYLSRSNNECMFHIEDNKIYKVIPVHDVKPLVEISMN
CCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCEEEEEEECCCCCCEEEEECC
ADDTIQIRFLGEAYISAKPIRDAVANYVTEWFDLTIDLAPFYTLAKHDVLLQRPIEQYYG
CCCEEEEEEECCHHHCCCCHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHCC
LRTLGIPDLFEALSWGIIGQQINLTYAYTLKRRLVETFGSYVEWNDRKHWIFPSPETIAN
EEECCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHCCCEEECCCEEEECCCCHHHHH
LHVEDLKNLKMTTRKCEYLIGIAKLITEGNLSKESLLQIQDVKQAEKRLTAIHGIGPWTA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
NYVLMRCLRFPSAFPIDDVGLHNAIKYLTGSESKPTKHEIKDFAVNWKNWESYATFYLWR
HHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHH
VLY
HCC
>Mature Secondary Structure 
TAEYSNALILTVPTEFSFQENLRYLSRSNNECMFHIEDNKIYKVIPVHDVKPLVEISMN
CCCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCEEEEEEECCCCCCEEEEECC
ADDTIQIRFLGEAYISAKPIRDAVANYVTEWFDLTIDLAPFYTLAKHDVLLQRPIEQYYG
CCCEEEEEEECCHHHCCCCHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHCC
LRTLGIPDLFEALSWGIIGQQINLTYAYTLKRRLVETFGSYVEWNDRKHWIFPSPETIAN
EEECCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHCCCEEECCCEEEECCCCHHHHH
LHVEDLKNLKMTTRKCEYLIGIAKLITEGNLSKESLLQIQDVKQAEKRLTAIHGIGPWTA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
NYVLMRCLRFPSAFPIDDVGLHNAIKYLTGSESKPTKHEIKDFAVNWKNWESYATFYLWR
HHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHH
VLY
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8376346; 9384377 [H]