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The map label for this gene is adaA [H]

Identifier: 218904867

GI number: 218904867

Start: 3580077

End: 3580673

Strand: Direct

Name: adaA [H]

Synonym: BCAH820_3751

Alternate gene names: 218904867

Gene position: 3580077-3580673 (Clockwise)

Preceding gene: 218904856

Following gene: 218904868

Centisome position: 67.51

GC content: 35.34

Gene sequence:

>597_bases
TTGCACAATGAAGGAGTTACGTTAACAAATGAGCATTGGCAAGCAATTATTCATAATGATTCTTCCTATGATAGTAAATT
CTTTTATGCCGTGAAATCAACCGGGGTTTTTTGCCGACCGTCATGCAAATCAAGAATACCGAATAAAAACAATGTACGAA
TTTTTCATCATGCAGAGCAAGCATTAAGTGAAAACTTTCGCCCGTGCAAACGTTGTAAACCAAATGGGCTCACTTTACCT
AATGAAGAGTGGGTAGAACAAATTAAAGAGTATATCGAAAAACACTTTGATGAAGCATTAACTCTAGACATACTTGCGGA
AATGTGCCATGGTAGCCCCTTTCATTTACAACGCACTTTCAAAAAAATGACAGGAATAAGTCCGATAGAATATATACAGC
AGTTCAGAATTGTTAAAGCAGCAGAACACCTTTTACACACAAATCAACCCATTAAAGAAATTAGTACTGCCGTCGGGATA
GAAAACCCAGAATATTTCGCAACTTTATTTAAAAAGAAAACTGGGTTTACCCCTACTGAATATCGAAAAAAGAATGAAAT
GAAAGAGGGATACAATAATGAATTCCTACAAAAATAA

Upstream 100 bases:

>100_bases
AAAACAATCATATTTTCACCTTTGTAATCTTGCTCTTACATTTATGAATACATACATACGCACTTTATTCAGGTTATACT
AATTTCAGAGGTGATGTTCT

Downstream 100 bases:

>100_bases
ATTTATATATTGGACGCTACTTACACATGCAAATTGGAAGTTTCATATTGCTGCAACTGAAAGTGGGCTATGTTTCATTG
GATCACAAGACGAACAATTT

Product: Ada regulatory protein/6-O-methylguanine-DNA methyltransferase

Products: DNA; protein S-methyl-L-cysteine

Alternate protein names: NA

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MHNEGVTLTNEHWQAIIHNDSSYDSKFFYAVKSTGVFCRPSCKSRIPNKNNVRIFHHAEQALSENFRPCKRCKPNGLTLP
NEEWVEQIKEYIEKHFDEALTLDILAEMCHGSPFHLQRTFKKMTGISPIEYIQQFRIVKAAEHLLHTNQPIKEISTAVGI
ENPEYFATLFKKKTGFTPTEYRKKNEMKEGYNNEFLQK

Sequences:

>Translated_198_residues
MHNEGVTLTNEHWQAIIHNDSSYDSKFFYAVKSTGVFCRPSCKSRIPNKNNVRIFHHAEQALSENFRPCKRCKPNGLTLP
NEEWVEQIKEYIEKHFDEALTLDILAEMCHGSPFHLQRTFKKMTGISPIEYIQQFRIVKAAEHLLHTNQPIKEISTAVGI
ENPEYFATLFKKKTGFTPTEYRKKNEMKEGYNNEFLQK
>Mature_198_residues
MHNEGVTLTNEHWQAIIHNDSSYDSKFFYAVKSTGVFCRPSCKSRIPNKNNVRIFHHAEQALSENFRPCKRCKPNGLTLP
NEEWVEQIKEYIEKHFDEALTLDILAEMCHGSPFHLQRTFKKMTGISPIEYIQQFRIVKAAEHLLHTNQPIKEISTAVGI
ENPEYFATLFKKKTGFTPTEYRKKNEMKEGYNNEFLQK

Specific function: Methylphosphotriester-DNA alkyltransferase is one of two genes required for the adaptive response of bacteria to alkylating agents. It accepts a methyl group from methylphospho-triesters and then acts as a transcriptional activator of the ada operon [H]

COG id: COG2169

COG function: function code F; Adenosine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=178, Percent_Identity=32.0224719101124, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI1790497, Length=106, Percent_Identity=28.3018867924528, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790857, Length=102, Percent_Identity=34.3137254901961, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1787987, Length=121, Percent_Identity=31.404958677686, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1790391, Length=104, Percent_Identity=31.7307692307692, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR016220 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF00165 HTH_AraC [H]

EC number: 2.1.1.63

Molecular weight: Translated: 23168; Mature: 23168

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNEGVTLTNEHWQAIIHNDSSYDSKFFYAVKSTGVFCRPSCKSRIPNKNNVRIFHHAEQ
CCCCCCEEEHHHHEEEEECCCCCCCEEEEEEECCCEEECCCHHHCCCCCCCEEEEHHHHH
ALSENFRPCKRCKPNGLTLPNEEWVEQIKEYIEKHFDEALTLDILAEMCHGSPFHLQRTF
HHHCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
KKMTGISPIEYIQQFRIVKAAEHLLHTNQPIKEISTAVGIENPEYFATLFKKKTGFTPTE
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCHHH
YRKKNEMKEGYNNEFLQK
HHHHHHHHCCCCHHHHCC
>Mature Secondary Structure
MHNEGVTLTNEHWQAIIHNDSSYDSKFFYAVKSTGVFCRPSCKSRIPNKNNVRIFHHAEQ
CCCCCCEEEHHHHEEEEECCCCCCCEEEEEEECCCEEECCCHHHCCCCCCCEEEEHHHHH
ALSENFRPCKRCKPNGLTLPNEEWVEQIKEYIEKHFDEALTLDILAEMCHGSPFHLQRTF
HHHCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
KKMTGISPIEYIQQFRIVKAAEHLLHTNQPIKEISTAVGIENPEYFATLFKKKTGFTPTE
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCHHH
YRKKNEMKEGYNNEFLQK
HHHHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA containing 6-O-methylguanine; protein L-cysteine

Specific reaction: DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2120677; 9384377; 1744039 [H]