Click here to switch to the map view.

The map label for this gene is blyA [H]

Identifier: 218902705

GI number: 218902705

Start: 1500302

End: 1500832

Strand: Direct

Name: blyA [H]

Synonym: BCAH820_1588

Alternate gene names: 218902705

Gene position: 1500302-1500832 (Clockwise)

Preceding gene: 218902704

Following gene: 218902706

Centisome position: 28.29

GC content: 37.85

Gene sequence:

>531_bases
ATGGCGATGTTTCCTATAGAGCGTAACTATATTGGATATGGAAGTTCACGGCCGGGAATTCCTCTTTCTAAAGTAAGGTT
TATTGTAAGCCATGATACGGGGAATCCTGGTAGCAATGCGATTGGGAATCGGGATTATTTTAATGAGATACAACCGAAAG
CTTCGGCACATACATTTATAGATGACAAAACAATACTAGAAATTATTCCTATAAATGAAGTTGCTTATCATGTTCGATAT
GGTGTGCCGACAGATAATGACTTGTATGGGTATGATGCCAATAAGGCTGCTATTGGAGTGGAGCTTTGTTATGGCGGTGA
TGTTAATTTTTGGGAAGCATATACCCGTTTTACGTGGTATCATGCGTATTTATGTCAAAACTTCGGCTTGAATCCGAAAA
AAGATATCGTATCACATAAAACACTTGATCCAGCCAGAAAGATTGATCCTGAAAATGTTTTAGGGAAACAAGGTATTAAA
TTTCAGCAGTTTTTAGCAGATGTCTATCGGATGTACGTTTCGTTTAGATGA

Upstream 100 bases:

>100_bases
TTCGATTTTCTCTTCGCGTAAGAAATATGCTATAAGGACATTGCTACCGAATACATATATAAAGAAGTAAGGTAATCTTC
TGAAGCGGGAGTTGGTAGTA

Downstream 100 bases:

>100_bases
CAAATATATGAAAAATGAATACAATAAAGATAGGGGCCTACTGCTGTAAGTAGGTTTTTTCTTGTTTTTATAAATAGTGT
ATTGGTAGAAGACTAGGAGT

Product: N-acetylmuramoyl-L-alanine amidase, family 2

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 176; Mature: 175

Protein sequence:

>176_residues
MAMFPIERNYIGYGSSRPGIPLSKVRFIVSHDTGNPGSNAIGNRDYFNEIQPKASAHTFIDDKTILEIIPINEVAYHVRY
GVPTDNDLYGYDANKAAIGVELCYGGDVNFWEAYTRFTWYHAYLCQNFGLNPKKDIVSHKTLDPARKIDPENVLGKQGIK
FQQFLADVYRMYVSFR

Sequences:

>Translated_176_residues
MAMFPIERNYIGYGSSRPGIPLSKVRFIVSHDTGNPGSNAIGNRDYFNEIQPKASAHTFIDDKTILEIIPINEVAYHVRY
GVPTDNDLYGYDANKAAIGVELCYGGDVNFWEAYTRFTWYHAYLCQNFGLNPKKDIVSHKTLDPARKIDPENVLGKQGIK
FQQFLADVYRMYVSFR
>Mature_175_residues
AMFPIERNYIGYGSSRPGIPLSKVRFIVSHDTGNPGSNAIGNRDYFNEIQPKASAHTFIDDKTILEIIPINEVAYHVRYG
VPTDNDLYGYDANKAAIGVELCYGGDVNFWEAYTRFTWYHAYLCQNFGLNPKKDIVSHKTLDPARKIDPENVLGKQGIKF
QQFLADVYRMYVSFR

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Involved in prophage SP-beta-mediated cell lysis [H]

COG id: COG5632

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 20085; Mature: 19954

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMFPIERNYIGYGSSRPGIPLSKVRFIVSHDTGNPGSNAIGNRDYFNEIQPKASAHTFI
CCCCCCCCCEECCCCCCCCCCHHHEEEEEEECCCCCCCCCCCCCHHHHHCCCCCCCCEEE
DDKTILEIIPINEVAYHVRYGVPTDNDLYGYDANKAAIGVELCYGGDVNFWEAYTRFTWY
CCCEEEEEECCCCEEEEEEECCCCCCCEECCCCCCEEEEEEEEECCCCHHHHHHHHHHHH
HAYLCQNFGLNPKKDIVSHKTLDPARKIDPENVLGKQGIKFQQFLADVYRMYVSFR
HHHHHHHCCCCCHHHHHCCCCCCHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AMFPIERNYIGYGSSRPGIPLSKVRFIVSHDTGNPGSNAIGNRDYFNEIQPKASAHTFI
CCCCCCCCEECCCCCCCCCCHHHEEEEEEECCCCCCCCCCCCCHHHHHCCCCCCCCEEE
DDKTILEIIPINEVAYHVRYGVPTDNDLYGYDANKAAIGVELCYGGDVNFWEAYTRFTWY
CCCEEEEEECCCCEEEEEEECCCCCCCEECCCCCCEEEEEEEEECCCCHHHHHHHHHHHH
HAYLCQNFGLNPKKDIVSHKTLDPARKIDPENVLGKQGIKFQQFLADVYRMYVSFR
HHHHHHHCCCCCHHHHHCCCCCCHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579063; 9384377 [H]