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The map label for this gene is hmp [H]

Identifier: 218902657

GI number: 218902657

Start: 1454446

End: 1455654

Strand: Direct

Name: hmp [H]

Synonym: BCAH820_1540

Alternate gene names: 218902657

Gene position: 1454446-1455654 (Clockwise)

Preceding gene: 218902656

Following gene: 218902659

Centisome position: 27.43

GC content: 34.33

Gene sequence:

>1209_bases
ATGTTAAGTGAAAAAACAATTGAAATCGTAAAGTCAACAGTACCATTATTACAGGAAAAAGGCGTTGAAATTACAACGAG
ATTTTATGAAATTTTATTTTCGGAACATCCGGAGTTATTGAATATTTTCAACCATACAAATCAGAAAAAGGGAAGACAAC
AACAAGCGTTAGCGAATGCTGTTTATGCAGCTGCAACGTACATTGATAATTTAGAAGCTATTATTCCAGTTGTAAAACAA
ATTGGTCATAAGCATAGAAGTTTAGGTATTAAAGCAGAGCATTATCCGATTGTAGGTACATGTTTATTACGTGCAATTAA
AGAGGTCGCAGGTGCACCGGATGAAGTATTAAACGCATGGGGAGAAGCATATGGTGTCATTGCTGATGCATTCATTAGCA
TTGAAGCAGAGATGTATGAAGAAGCGGCACATAAAGAAGGTGGATGGAAAGATTTCCGTAACTTTGTGGTTGTTAAAAAA
GTGAAGGAAAGCGATGTTATCACGTCATTTTATTTAAAACCTGAAGATGGAGGGAAAGTTTCTTCATTCATTCCAGGACA
ATATGTAACGGTTCAAATAAATATCGAAGGTGAAACATATACACATAATCGTCAATATAGCTTATCAGATGCTCCTGGAA
AAGAATATTATCGTATTAGTGTCAAGAAAGAAAAAGGTGTAGATACGCCGGATGGTAAAGTATCTAATTACTTACATGAT
CATGTAAAAGAAGGTGATATGTTACCAGTAAGTGCACCAGCAGGAGATTTCGTATTAAATATGGATTCAACTTTACCAGT
TGTCTTAATTAGTGGTGGAGTAGGTATTACACCAATGATGAGTATGCTAAATACGTTAATTGAACAAGATTCAAAACGTA
ATGTATGTTTTGTTCATGCAGCGATAAATAGTAATACACATGCAATGAAAGAACACGTTGAGGCAGTAGATAATGAATAC
GAACAAGTTAAAGCATATACTTGTTATTCTGCACCAACTGGAAAAGATTTAGAAATGAAGAACTTTGATAAAGAAGGTTT
TGTGGAAAGAGAATGGTTACAAACTATTATTCCGACAACTGAAGCAGAGTTTTATTTCTGTGGTCCAGTACCATTTATGA
AACATATAAATGCTGTACTAACTGATTTAGGTGTGAAACAAGAACATATTCATTATGAATTTTTCGGCCCAGCAGCAAGC
TTACAATAA

Upstream 100 bases:

>100_bases
TTTCACAAAAACACCGTGTTAATAGTGATTAAAATCACAATCTGTATTATACAATTCAGTTAAACTAAAAACAGTTAAGG
AAATAGAGGAGTGGGATACA

Downstream 100 bases:

>100_bases
ATTGAGTTTTAATAAGCAAAAAAAAACGAGGTGTTATTTCACTTCGTTTTTTATTTGTTCTCGTAATAGTCGATTTACTG
TTTCGCGGCGTATACCGATC

Product: nitric oxide dioxygenase

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MLSEKTIEIVKSTVPLLQEKGVEITTRFYEILFSEHPELLNIFNHTNQKKGRQQQALANAVYAAATYIDNLEAIIPVVKQ
IGHKHRSLGIKAEHYPIVGTCLLRAIKEVAGAPDEVLNAWGEAYGVIADAFISIEAEMYEEAAHKEGGWKDFRNFVVVKK
VKESDVITSFYLKPEDGGKVSSFIPGQYVTVQINIEGETYTHNRQYSLSDAPGKEYYRISVKKEKGVDTPDGKVSNYLHD
HVKEGDMLPVSAPAGDFVLNMDSTLPVVLISGGVGITPMMSMLNTLIEQDSKRNVCFVHAAINSNTHAMKEHVEAVDNEY
EQVKAYTCYSAPTGKDLEMKNFDKEGFVEREWLQTIIPTTEAEFYFCGPVPFMKHINAVLTDLGVKQEHIHYEFFGPAAS
LQ

Sequences:

>Translated_402_residues
MLSEKTIEIVKSTVPLLQEKGVEITTRFYEILFSEHPELLNIFNHTNQKKGRQQQALANAVYAAATYIDNLEAIIPVVKQ
IGHKHRSLGIKAEHYPIVGTCLLRAIKEVAGAPDEVLNAWGEAYGVIADAFISIEAEMYEEAAHKEGGWKDFRNFVVVKK
VKESDVITSFYLKPEDGGKVSSFIPGQYVTVQINIEGETYTHNRQYSLSDAPGKEYYRISVKKEKGVDTPDGKVSNYLHD
HVKEGDMLPVSAPAGDFVLNMDSTLPVVLISGGVGITPMMSMLNTLIEQDSKRNVCFVHAAINSNTHAMKEHVEAVDNEY
EQVKAYTCYSAPTGKDLEMKNFDKEGFVEREWLQTIIPTTEAEFYFCGPVPFMKHINAVLTDLGVKQEHIHYEFFGPAAS
LQ
>Mature_402_residues
MLSEKTIEIVKSTVPLLQEKGVEITTRFYEILFSEHPELLNIFNHTNQKKGRQQQALANAVYAAATYIDNLEAIIPVVKQ
IGHKHRSLGIKAEHYPIVGTCLLRAIKEVAGAPDEVLNAWGEAYGVIADAFISIEAEMYEEAAHKEGGWKDFRNFVVVKK
VKESDVITSFYLKPEDGGKVSSFIPGQYVTVQINIEGETYTHNRQYSLSDAPGKEYYRISVKKEKGVDTPDGKVSNYLHD
HVKEGDMLPVSAPAGDFVLNMDSTLPVVLISGGVGITPMMSMLNTLIEQDSKRNVCFVHAAINSNTHAMKEHVEAVDNEY
EQVKAYTCYSAPTGKDLEMKNFDKEGFVEREWLQTIIPTTEAEFYFCGPVPFMKHINAVLTDLGVKQEHIHYEFFGPAAS
LQ

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=397, Percent_Identity=45.5919395465995, Blast_Score=354, Evalue=5e-99,
Organism=Escherichia coli, GI1787658, Length=252, Percent_Identity=23.4126984126984, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1788104, Length=249, Percent_Identity=26.9076305220884, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787098, Length=219, Percent_Identity=27.3972602739726, Blast_Score=69, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6321673, Length=413, Percent_Identity=42.3728813559322, Blast_Score=274, Evalue=1e-74,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR001709
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 44970; Mature: 44970

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS01033 GLOBIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSEKTIEIVKSTVPLLQEKGVEITTRFYEILFSEHPELLNIFNHTNQKKGRQQQALANA
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCHHCCHHHHHHHHH
VYAAATYIDNLEAIIPVVKQIGHKHRSLGIKAEHYPIVGTCLLRAIKEVAGAPDEVLNAW
HHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
GEAYGVIADAFISIEAEMYEEAAHKEGGWKDFRNFVVVKKVKESDVITSFYLKPEDGGKV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCEEEEEECCCCCCEEEEEECCCCCCCC
SSFIPGQYVTVQINIEGETYTHNRQYSLSDAPGKEYYRISVKKEKGVDTPDGKVSNYLHD
CCCCCCEEEEEEEEECCCEEECCCEEECCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHH
HVKEGDMLPVSAPAGDFVLNMDSTLPVVLISGGVGITPMMSMLNTLIEQDSKRNVCFVHA
HHCCCCEEEECCCCCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEE
AINSNTHAMKEHVEAVDNEYEQVKAYTCYSAPTGKDLEMKNFDKEGFVEREWLQTIIPTT
EECCCHHHHHHHHHHHCCHHHHHEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCC
EAEFYFCGPVPFMKHINAVLTDLGVKQEHIHYEFFGPAASLQ
CCCEEEECCCHHHHHHHHHHHHCCCCHHHEEEEEECCCCCCC
>Mature Secondary Structure
MLSEKTIEIVKSTVPLLQEKGVEITTRFYEILFSEHPELLNIFNHTNQKKGRQQQALANA
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCHHCCHHHHHHHHH
VYAAATYIDNLEAIIPVVKQIGHKHRSLGIKAEHYPIVGTCLLRAIKEVAGAPDEVLNAW
HHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
GEAYGVIADAFISIEAEMYEEAAHKEGGWKDFRNFVVVKKVKESDVITSFYLKPEDGGKV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCEEEEEECCCCCCEEEEEECCCCCCCC
SSFIPGQYVTVQINIEGETYTHNRQYSLSDAPGKEYYRISVKKEKGVDTPDGKVSNYLHD
CCCCCCEEEEEEEEECCCEEECCCEEECCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHH
HVKEGDMLPVSAPAGDFVLNMDSTLPVVLISGGVGITPMMSMLNTLIEQDSKRNVCFVHA
HHCCCCEEEECCCCCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEE
AINSNTHAMKEHVEAVDNEYEQVKAYTCYSAPTGKDLEMKNFDKEGFVEREWLQTIIPTT
EECCCHHHHHHHHHHHCCHHHHHEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCC
EAEFYFCGPVPFMKHINAVLTDLGVKQEHIHYEFFGPAASLQ
CCCEEEECCCHHHHHHHHHHHHCCCCHHHEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]