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The map label for this gene is liaR [H]

Identifier: 218902648

GI number: 218902648

Start: 1447130

End: 1447762

Strand: Direct

Name: liaR [H]

Synonym: BCAH820_1531

Alternate gene names: 218902648

Gene position: 1447130-1447762 (Clockwise)

Preceding gene: 218902647

Following gene: 218902649

Centisome position: 27.29

GC content: 37.18

Gene sequence:

>633_bases
ATGATAAAAGTATTACTAGTGGATGATCATGAAATGGTTCGAATGGGTGTATCAGCATATTTATCAACGCAGCCAGATAT
TGAAGTAGTTGGAGAAGCTGAAAATGGAAGAAAAGGTTCAGAGTTAGCACTGCAATTAAGACCGGATATTATTTTAATGG
ATCTTGTCATGGACGAGATGGATGGTGTTGAAGCAACACGTGCTATTATTCAAGAATGGCCAGAAGCAAAAATTGTAKTT
GTAACCAGCTTTTTAGATGATGAGAAATTGTACCCTGTTATTGAGGCCGGAGCGACCAGTTATTTATTAAAAACATCAAG
AGCGAGTGATATAGCAGACGCTGTACGGGCTACTTATGATGGAGAAACGGTATTAGAGCCAAAAGTTACTGGTAAAATGA
TGTCTCGTATGCGTCAGAAGAAAGAACAGCCTTTGCATGAGGAGTTAACAGAAAGAGAGTCTGAAATTTTGTTATTAATT
GCAGAGGGGAAAAGTAATCAAGAGATTGCAGATGAGTTGTTTATTGCTCTTAAAACAGTAAAGACACATGTGAGTAATAT
ATTAAATAAGCTAAATGTAAGTGACCGGACACAGGCGGTTATTTATGCGTTTAGACATCAATTGACGAAATGA

Upstream 100 bases:

>100_bases
TTCATGAAATTGGTGGTACATTAAAAGTGCTTAGTTTTCCAAATAAAGGTACACAAATAGAAGTAAAAGTACCGATTATG
ATTGAGAGAGGGGGAGAATC

Downstream 100 bases:

>100_bases
TACAGTGAAACTTTAATCAGTGGGGAGCTATTCCCACTGATTATTAGTTTGCACCGAATCATTTTATTGACGCTAATATA
TGTAAGCGTTATCATTAAGT

Product: DNA-binding response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MIKVLLVDDHEMVRMGVSAYLSTQPDIEVVGEAENGRKGSELALQLRPDIILMDLVMDEMDGVEATRAIIQEWPEAKIVX
VTSFLDDEKLYPVIEAGATSYLLKTSRASDIADAVRATYDGETVLEPKVTGKMMSRMRQKKEQPLHEELTERESEILLLI
AEGKSNQEIADELFIALKTVKTHVSNILNKLNVSDRTQAVIYAFRHQLTK

Sequences:

>Translated_210_residues
MIKVLLVDDHEMVRMGVSAYLSTQPDIEVVGEAENGRKGSELALQLRPDIILMDLVMDEMDGVEATRAIIQEWPEAKIVX
VTSFLDDEKLYPVIEAGATSYLLKTSRASDIADAVRATYDGETVLEPKVTGKMMSRMRQKKEQPLHEELTERESEILLLI
AEGKSNQEIADELFIALKTVKTHVSNILNKLNVSDRTQAVIYAFRHQLTK
>Mature_210_residues
MIKVLLVDDHEMVRMGVSAYLSTQPDIEVVGEAENGRKGSELALQLRPDIILMDLVMDEMDGVEATRAIIQEWPEAKIVX
VTSFLDDEKLYPVIEAGATSYLLKTSRASDIADAVRATYDGETVLEPKVTGKMMSRMRQKKEQPLHEELTERESEILLLI
AEGKSNQEIADELFIALKTVKTHVSNILNKLNVSDRTQAVIYAFRHQLTK

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=211, Percent_Identity=33.175355450237, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1788521, Length=206, Percent_Identity=36.4077669902913, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787473, Length=207, Percent_Identity=36.7149758454106, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1790102, Length=200, Percent_Identity=31, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1786747, Length=208, Percent_Identity=29.3269230769231, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1788712, Length=207, Percent_Identity=22.7053140096618, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI87082052, Length=104, Percent_Identity=37.5, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23556; Mature: 23556

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVLLVDDHEMVRMGVSAYLSTQPDIEVVGEAENGRKGSELALQLRPDIILMDLVMDEM
CEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEECCCEEHHHHHHHHH
DGVEATRAIIQEWPEAKIVXVTSFLDDEKLYPVIEAGATSYLLKTSRASDIADAVRATYD
CCHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHCCCHHHHHHHCCHHHHHHHHHHHCC
GETVLEPKVTGKMMSRMRQKKEQPLHEELTERESEILLLIAEGKSNQEIADELFIALKTV
CCEEECCHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHH
KTHVSNILNKLNVSDRTQAVIYAFRHQLTK
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIKVLLVDDHEMVRMGVSAYLSTQPDIEVVGEAENGRKGSELALQLRPDIILMDLVMDEM
CEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEECCCEEHHHHHHHHH
DGVEATRAIIQEWPEAKIVXVTSFLDDEKLYPVIEAGATSYLLKTSRASDIADAVRATYD
CCHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHCCCHHHHHHHCCHHHHHHHHHHHCC
GETVLEPKVTGKMMSRMRQKKEQPLHEELTERESEILLLIAEGKSNQEIADELFIALKTV
CCEEECCHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHH
KTHVSNILNKLNVSDRTQAVIYAFRHQLTK
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]