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The map label for this gene is lmrB [H]

Identifier: 218902146

GI number: 218902146

Start: 997721

End: 999196

Strand: Direct

Name: lmrB [H]

Synonym: BCAH820_1028

Alternate gene names: 218902146

Gene position: 997721-999196 (Clockwise)

Preceding gene: 218902144

Following gene: 218902151

Centisome position: 18.82

GC content: 36.86

Gene sequence:

>1476_bases
ATGAATATGACAACTAAGAGCCCTGCAAGTGGCAAAGTTGATACTTCTAATATGAAACATACCCCCATTTTAATCGCATT
ACTTTTAGGGGCAATGGTTGCATTATTAAACGAAACGTTACTTGGTAATGCATTAACAGTATTAATGAAAGAATTTGACG
TAACAGCTTCAACGATTCAATGGCTTTCTACAGCTTACATGTTAGTAGTTGGGGTTTTAGTCCCAATTACAGCGTTACTT
CAGCAATGGCTTACAACGAGACAGATGTTTTTGATCGCTATGGTAACATTTTTAGTCGGTACATTAATTGCGGGATTTGC
ACCGACATTTTCTGTTTTATTAGTAGGCCGTATCGTCCAAGCTGTAGCGACGGGCTTAATTTCACCGTTATTAATGAATA
CAATTTTAATTATTTGTCCACCTGAAAAACGTGGTGCCACAATGGGATTAATCGCACTTGTGATGATGTCCGCTCCGGCA
ATTGGTCCTACATTATCTGGAGTAATTGTGGATTCACTCAATTGGCGCTGGTTATTCTATATTGTAATTCCTATTGTAAT
CATTTCAATTATGATTGGGGTGAAGTACATTCAAAATGTTTCAGAGTTAACAAGACCGAAAGTAGATTATCCATCTATCC
TTTTATCGACATTAGGATTTGGCGGGCTTGTGTATAGCTTTAGTGCATCAGGTGATTTAGGATGGTCTGATACGAAAGTG
TATAGTGCTTTAATTGTTGGGCTTATCTCATTATGTATTTTCGTCGTGCGACAATTAAAAATTGATAATCCAATTTTAGA
ATTACGAGCATTTAAAGTTCCGATGTTTACATTATCAGTCGGATTAATAGTCATTGTGATGATGTCATTATTTTCAACGA
TGACTTTATTACCGATGTTCCTGCAAACAGTTTTACTCGTTACAGCTTTTAAATCAGGAATCATCATGCTTCCAGGAAGT
GTTATTAGCGCCATAATGGGACCAATCGCAGGTAAACTATTTGATAAATTTAGTCCGAAAGTTATTATCGTTCCCGGCAT
TGTGTTAGTAGGAATTGCAATGTTCTTATTTAAAGGCATTACTCCTGATACATCAATAGTACAAATTATTGTGATGCATA
GCGTATTAATGGTTGGACTCATGTTTGTCATGACAGCACAGACATACGGCTTAAATCAATTAACACCAGATTTATATCCG
CACGGAACAGCGTTGTTTAATACTCTGCAGCAAGTAGCTGGCGCAATTGGTACAGCTATCTTTATTTCGAAAATGTCTTC
AGGAACAGCTAGCTATATGGAAAGCTCCGCAAATCCAATGGACCCAGTAGAGAAGTTAAACGGTTTAACGTCAGGGTTCC
AAGGTGCATTTGCACTTGGGTTAATCTTTATTGTTGTTGCTTTTATTGTATCCCTATTCTTAAAAGAAGAGAAAAAGGGA
GTAGAAGTAAAGGGAGTTTTACAGAACGAAAATTAA

Upstream 100 bases:

>100_bases
AAATTGATTCTCCCTTTTTAATCTACATATGTATATTATATTACATGTGTTTGGGTTTATATAAAGCTTTTTGATTAAGA
AATAGGAGGAGAATCTGCAA

Downstream 100 bases:

>100_bases
GTTCTGTATAAAAAGACATCTTTCAGAGGGAAAGATGTCTTTTACATTTCCTCCGTAACATCATACCGAAGCAACTCTCC
CTGATCATAAAACCTCTCAA

Product: drug resistance transporter, EmrB/QacA family

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MNMTTKSPASGKVDTSNMKHTPILIALLLGAMVALLNETLLGNALTVLMKEFDVTASTIQWLSTAYMLVVGVLVPITALL
QQWLTTRQMFLIAMVTFLVGTLIAGFAPTFSVLLVGRIVQAVATGLISPLLMNTILIICPPEKRGATMGLIALVMMSAPA
IGPTLSGVIVDSLNWRWLFYIVIPIVIISIMIGVKYIQNVSELTRPKVDYPSILLSTLGFGGLVYSFSASGDLGWSDTKV
YSALIVGLISLCIFVVRQLKIDNPILELRAFKVPMFTLSVGLIVIVMMSLFSTMTLLPMFLQTVLLVTAFKSGIIMLPGS
VISAIMGPIAGKLFDKFSPKVIIVPGIVLVGIAMFLFKGITPDTSIVQIIVMHSVLMVGLMFVMTAQTYGLNQLTPDLYP
HGTALFNTLQQVAGAIGTAIFISKMSSGTASYMESSANPMDPVEKLNGLTSGFQGAFALGLIFIVVAFIVSLFLKEEKKG
VEVKGVLQNEN

Sequences:

>Translated_491_residues
MNMTTKSPASGKVDTSNMKHTPILIALLLGAMVALLNETLLGNALTVLMKEFDVTASTIQWLSTAYMLVVGVLVPITALL
QQWLTTRQMFLIAMVTFLVGTLIAGFAPTFSVLLVGRIVQAVATGLISPLLMNTILIICPPEKRGATMGLIALVMMSAPA
IGPTLSGVIVDSLNWRWLFYIVIPIVIISIMIGVKYIQNVSELTRPKVDYPSILLSTLGFGGLVYSFSASGDLGWSDTKV
YSALIVGLISLCIFVVRQLKIDNPILELRAFKVPMFTLSVGLIVIVMMSLFSTMTLLPMFLQTVLLVTAFKSGIIMLPGS
VISAIMGPIAGKLFDKFSPKVIIVPGIVLVGIAMFLFKGITPDTSIVQIIVMHSVLMVGLMFVMTAQTYGLNQLTPDLYP
HGTALFNTLQQVAGAIGTAIFISKMSSGTASYMESSANPMDPVEKLNGLTSGFQGAFALGLIFIVVAFIVSLFLKEEKKG
VEVKGVLQNEN
>Mature_491_residues
MNMTTKSPASGKVDTSNMKHTPILIALLLGAMVALLNETLLGNALTVLMKEFDVTASTIQWLSTAYMLVVGVLVPITALL
QQWLTTRQMFLIAMVTFLVGTLIAGFAPTFSVLLVGRIVQAVATGLISPLLMNTILIICPPEKRGATMGLIALVMMSAPA
IGPTLSGVIVDSLNWRWLFYIVIPIVIISIMIGVKYIQNVSELTRPKVDYPSILLSTLGFGGLVYSFSASGDLGWSDTKV
YSALIVGLISLCIFVVRQLKIDNPILELRAFKVPMFTLSVGLIVIVMMSLFSTMTLLPMFLQTVLLVTAFKSGIIMLPGS
VISAIMGPIAGKLFDKFSPKVIIVPGIVLVGIAMFLFKGITPDTSIVQIIVMHSVLMVGLMFVMTAQTYGLNQLTPDLYP
HGTALFNTLQQVAGAIGTAIFISKMSSGTASYMESSANPMDPVEKLNGLTSGFQGAFALGLIFIVVAFIVSLFLKEEKKG
VEVKGVLQNEN

Specific function: Proton-dependent transporter. May mediate the efflux of lincomycin [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=430, Percent_Identity=25.1162790697674, Blast_Score=141, Evalue=8e-35,
Organism=Escherichia coli, GI1788710, Length=431, Percent_Identity=24.1299303944316, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI87081983, Length=333, Percent_Identity=24.3243243243243, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1790195, Length=390, Percent_Identity=24.6153846153846, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1788392, Length=314, Percent_Identity=25.4777070063694, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323735, Length=317, Percent_Identity=25.2365930599369, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6325455, Length=135, Percent_Identity=28.8888888888889, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319770, Length=320, Percent_Identity=22.8125, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321663, Length=136, Percent_Identity=30.8823529411765, Blast_Score=67, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52758; Mature: 52758

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMTTKSPASGKVDTSNMKHTPILIALLLGAMVALLNETLLGNALTVLMKEFDVTASTIQ
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
WLSTAYMLVVGVLVPITALLQQWLTTRQMFLIAMVTFLVGTLIAGFAPTFSVLLVGRIVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVATGLISPLLMNTILIICPPEKRGATMGLIALVMMSAPAIGPTLSGVIVDSLNWRWLFY
HHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHH
IVIPIVIISIMIGVKYIQNVSELTRPKVDYPSILLSTLGFGGLVYSFSASGDLGWSDTKV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHEEECCCCCCCCCHHHH
YSALIVGLISLCIFVVRQLKIDNPILELRAFKVPMFTLSVGLIVIVMMSLFSTMTLLPMF
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQTVLLVTAFKSGIIMLPGSVISAIMGPIAGKLFDKFSPKVIIVPGIVLVGIAMFLFKGI
HHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCC
TPDTSIVQIIVMHSVLMVGLMFVMTAQTYGLNQLTPDLYPHGTALFNTLQQVAGAIGTAI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FISKMSSGTASYMESSANPMDPVEKLNGLTSGFQGAFALGLIFIVVAFIVSLFLKEEKKG
HHHHHCCCHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VEVKGVLQNEN
CEEEEEECCCC
>Mature Secondary Structure
MNMTTKSPASGKVDTSNMKHTPILIALLLGAMVALLNETLLGNALTVLMKEFDVTASTIQ
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
WLSTAYMLVVGVLVPITALLQQWLTTRQMFLIAMVTFLVGTLIAGFAPTFSVLLVGRIVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVATGLISPLLMNTILIICPPEKRGATMGLIALVMMSAPAIGPTLSGVIVDSLNWRWLFY
HHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHH
IVIPIVIISIMIGVKYIQNVSELTRPKVDYPSILLSTLGFGGLVYSFSASGDLGWSDTKV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHEEECCCCCCCCCHHHH
YSALIVGLISLCIFVVRQLKIDNPILELRAFKVPMFTLSVGLIVIVMMSLFSTMTLLPMF
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQTVLLVTAFKSGIIMLPGSVISAIMGPIAGKLFDKFSPKVIIVPGIVLVGIAMFLFKGI
HHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCC
TPDTSIVQIIVMHSVLMVGLMFVMTAQTYGLNQLTPDLYPHGTALFNTLQQVAGAIGTAI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FISKMSSGTASYMESSANPMDPVEKLNGLTSGFQGAFALGLIFIVVAFIVSLFLKEEKKG
HHHHHCCCHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VEVKGVLQNEN
CEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377 [H]