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The map label for this gene is yhaR [H]

Identifier: 218902097

GI number: 218902097

Start: 948121

End: 948909

Strand: Direct

Name: yhaR [H]

Synonym: BCAH820_0979

Alternate gene names: 218902097

Gene position: 948121-948909 (Clockwise)

Preceding gene: 218902093

Following gene: 218902098

Centisome position: 17.88

GC content: 39.16

Gene sequence:

>789_bases
ATGAAAGTAACAAGTAAAACAGAATCTGTCATTGTGAAATATGAAGGGCACGTTGCAACGGTTATGGTCAATCGCCCAGA
GGTGTTAAATGCATTAGATGAGCCAACGTTAAAAGAGCTATTACAAAAACTGAAAGAAGTAGCGGAGAGTTCTGCACATA
TTGTTGTGTTATGCGGGAACGGCCGTGGTTTTTCTGCGGGTGGAGATATTAAATCGATGCTTTCAAGTAATGATGAAAGC
AAGTTTGATGGTATTATGAATACTATTTCTGAAGTCGTTGTGACTTTATATACGATGCCAAAGCTCGTTATTAGTGCGAT
TCATGGACCAACTGCTGGCCTTGGATTAAGCATTGCGCTAACAGCTGACTATGTGATGGCAGATATATCATCCATTATTG
CGATGAACTTTATTGGAATCGCTTTAATTCCCGATGGCGGTGGCCATTTCTTCCTTCAAAAGCGCGTTGGTGAAAATATG
ACGAAGCAAATTATTTGGGAAGGAAAGAAATTATCAGCGACAGAAGCGCTTGATATCGGCTTAATTGATGAAGTAATTGG
CGAGGATTTCCAAACAGCTGTGAAGCAAAAGATTAGTGAATGGTCACAAAAACCGATTAAAGCGATGATTCAAACGAAAC
AAATTTTATGTGAAGTAAATCGCTCCAATTTAGAACAAACATTGCAGCTTGAAAAACGCGGTCAATATGCGATGAGACAA
ACTGCGGATCATAAGGAAGGTATTGCTGCATTTTTAGAAAAACGTTTGCCGGCATTTAAAGGAGAATAA

Upstream 100 bases:

>100_bases
TCGTGATTATTAGACAGAAAATTAAGAAAAAAGAAGGGTTTTTCACCTCCTTTCTATAATTATATAAGTATAATCTTTGT
GTAGAAAAGAGGGGTATATA

Downstream 100 bases:

>100_bases
AGTGTAATGATAGTTTTACTAACAGTTAATTTAAGGTAAAGGGGAGAAAGTGCTAACACATTTTAGTATAATTGTGTTAG
CACTTTTTTTAGGAGTAATA

Product: enoyl-CoA hydratase

Products: crotonoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MKVTSKTESVIVKYEGHVATVMVNRPEVLNALDEPTLKELLQKLKEVAESSAHIVVLCGNGRGFSAGGDIKSMLSSNDES
KFDGIMNTISEVVVTLYTMPKLVISAIHGPTAGLGLSIALTADYVMADISSIIAMNFIGIALIPDGGGHFFLQKRVGENM
TKQIIWEGKKLSATEALDIGLIDEVIGEDFQTAVKQKISEWSQKPIKAMIQTKQILCEVNRSNLEQTLQLEKRGQYAMRQ
TADHKEGIAAFLEKRLPAFKGE

Sequences:

>Translated_262_residues
MKVTSKTESVIVKYEGHVATVMVNRPEVLNALDEPTLKELLQKLKEVAESSAHIVVLCGNGRGFSAGGDIKSMLSSNDES
KFDGIMNTISEVVVTLYTMPKLVISAIHGPTAGLGLSIALTADYVMADISSIIAMNFIGIALIPDGGGHFFLQKRVGENM
TKQIIWEGKKLSATEALDIGLIDEVIGEDFQTAVKQKISEWSQKPIKAMIQTKQILCEVNRSNLEQTLQLEKRGQYAMRQ
TADHKEGIAAFLEKRLPAFKGE
>Mature_262_residues
MKVTSKTESVIVKYEGHVATVMVNRPEVLNALDEPTLKELLQKLKEVAESSAHIVVLCGNGRGFSAGGDIKSMLSSNDES
KFDGIMNTISEVVVTLYTMPKLVISAIHGPTAGLGLSIALTADYVMADISSIIAMNFIGIALIPDGGGHFFLQKRVGENM
TKQIIWEGKKLSATEALDIGLIDEVIGEDFQTAVKQKISEWSQKPIKAMIQTKQILCEVNRSNLEQTLQLEKRGQYAMRQ
TADHKEGIAAFLEKRLPAFKGE

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=269, Percent_Identity=32.7137546468402, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI45643119, Length=223, Percent_Identity=25.1121076233184, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI260275230, Length=223, Percent_Identity=25.1121076233184, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI260274832, Length=223, Percent_Identity=25.1121076233184, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI4757966, Length=181, Percent_Identity=25.9668508287293, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI51538581, Length=181, Percent_Identity=25.9668508287293, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI25453481, Length=181, Percent_Identity=25.9668508287293, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI51538573, Length=181, Percent_Identity=25.9668508287293, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI4757968, Length=181, Percent_Identity=25.414364640884, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI49355787, Length=181, Percent_Identity=25.414364640884, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI116235487, Length=180, Percent_Identity=27.2222222222222, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI68989263, Length=212, Percent_Identity=26.8867924528302, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI70995211, Length=262, Percent_Identity=24.4274809160305, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=255, Percent_Identity=28.6274509803922, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1787660, Length=260, Percent_Identity=31.1538461538462, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI87082183, Length=254, Percent_Identity=25.9842519685039, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI221142681, Length=269, Percent_Identity=24.5353159851301, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788597, Length=265, Percent_Identity=23.0188679245283, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788682, Length=176, Percent_Identity=25, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI25145438, Length=250, Percent_Identity=32.4, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17554946, Length=254, Percent_Identity=29.9212598425197, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17535521, Length=254, Percent_Identity=26.3779527559055, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25144157, Length=186, Percent_Identity=27.9569892473118, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25144160, Length=181, Percent_Identity=28.7292817679558, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17536985, Length=212, Percent_Identity=26.8867924528302, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI20129971, Length=249, Percent_Identity=29.718875502008, Blast_Score=100, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24653477, Length=249, Percent_Identity=29.718875502008, Blast_Score=100, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24654903, Length=239, Percent_Identity=22.5941422594142, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24653139, Length=274, Percent_Identity=23.7226277372263, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28571730, Length=161, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28571729, Length=161, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.55

Molecular weight: Translated: 28650; Mature: 28650

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVTSKTESVIVKYEGHVATVMVNRPEVLNALDEPTLKELLQKLKEVAESSAHIVVLCGN
CCCCCCCCEEEEEECCEEEEEEECCHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECC
GRGFSAGGDIKSMLSSNDESKFDGIMNTISEVVVTLYTMPKLVISAIHGPTAGLGLSIAL
CCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEH
TADYVMADISSIIAMNFIGIALIPDGGGHFFLQKRVGENMTKQIIWEGKKLSATEALDIG
HHHHHHHHHHHHHHHHHHEEEEEECCCCCEEHHHHHCCHHHHHHHHCCCCCCHHHHHHHH
LIDEVIGEDFQTAVKQKISEWSQKPIKAMIQTKQILCEVNRSNLEQTLQLEKRGQYAMRQ
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCHHHHHH
TADHKEGIAAFLEKRLPAFKGE
HCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKVTSKTESVIVKYEGHVATVMVNRPEVLNALDEPTLKELLQKLKEVAESSAHIVVLCGN
CCCCCCCCEEEEEECCEEEEEEECCHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECC
GRGFSAGGDIKSMLSSNDESKFDGIMNTISEVVVTLYTMPKLVISAIHGPTAGLGLSIAL
CCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEH
TADYVMADISSIIAMNFIGIALIPDGGGHFFLQKRVGENMTKQIIWEGKKLSATEALDIG
HHHHHHHHHHHHHHHHHHEEEEEECCCCCEEHHHHHCCHHHHHHHHCCCCCCHHHHHHHH
LIDEVIGEDFQTAVKQKISEWSQKPIKAMIQTKQILCEVNRSNLEQTLQLEKRGQYAMRQ
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCHHHHHH
TADHKEGIAAFLEKRLPAFKGE
HCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3R)-3-hydroxybutanoyl-CoA

Specific reaction: (3R)-3-hydroxybutanoyl-CoA = crotonoyl-CoA + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377; 9579061 [H]