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The map label for this gene is napA [H]

Identifier: 218902033

GI number: 218902033

Start: 872082

End: 873209

Strand: Direct

Name: napA [H]

Synonym: BCAH820_0915

Alternate gene names: 218902033

Gene position: 872082-873209 (Clockwise)

Preceding gene: 218902031

Following gene: 218902034

Centisome position: 16.45

GC content: 35.9

Gene sequence:

>1128_bases
ATGCTATTTTATTTTGAACTTGTCGTCATTTTACTTTGTACGAAATTAGCTGGTGATATTAGTGTGAGACTCGGCCAGCC
TTCTGTACTTGGGAAATTAATTGTTGGTATTATTATCGGTCCAGCTGTACTCGGTATTATTAATAGTTCTGAACTTATTG
ATGAATTGAGTGAGATTGGTGTTTTACTACTTATGTTTATGGCGGGACTTGAAACAGATTTAGAAGAATTAAATCGGAAT
TTGAAATCATCATTTGCAGTAGCGGCTGGTGGAATTATTTTTCCTTTCATCGGTGGTTATGTAACAGGATTGCTATTTGG
ATTAATACAATCTCATGCAATTTTTTTAGGATTATTACTTTGCGCAACGTCAGTTAGTATTACGGTACAAACGCTAAGAG
ATTTAGGGAAAATGAATACGAGAGAAAGTACAACGATTTTAGGTGCAGCCGTATTTGATGATGTCATCGTCGTTATTTTA
TTAGCGTTTGTTATGAGCTTTTTAGGAACGCAAGATGTGAATATAACACTTGTTATCGTAAAGAAAATTATTTTCTTTGT
CAGCATTGTTTTTATCGCATGGAAAGTTGTTCCGTGGATTATGAAAATGCTTGTGCCACTTCGTGTAACAGAAGCGTTAA
TTAGCGCGGCTCTTATTATTTGTTTTTCTTTTTCGTATTATAGTGAAATGATGGGGATTGCAGGTATTATTGGAGCATTT
GCAGCTGGAATTGCCATTTCGCAAACAGAGTATAAGCACGAAGTAGAACATAAAATTGAACCGATTGCATATGCGATATT
TGTACCAGTGTTCTTCGTAAGTATTGGAATGGAAATTACATTTCAAGGTATCGGAAGCCAGCTTTGGTTCATTATCATCA
TGACGCTTATTGCGATTTTCACAAAGTTAATTGGATCAGGTTTAGGAGCAAGACTAACAGGATTCAACCTTCAATCTTCT
ATTAGTATTGGTGCGGGAATGGTATCGCGTGGTGAAGTAGCACTTATTATTGCAGCAAATGGACTGGCGGCGAATTTACT
AGCGAAAGAAAATTTCACAGCGATTGTTATTGTTGTTATATTAACGACGATTATTACGCCGCCGCTTTTAAAGAAATATT
TCGTGTAA

Upstream 100 bases:

>100_bases
CGAATGAATAGGGGAAACTACTTGCAGGAATGGACGGGGATTACCAATCTATTCGGATGGAGAAAACCCACTGATTAAAG
TTAAAGTAGGAGAAGATGCT

Downstream 100 bases:

>100_bases
AGAGGAAGAGCTTATCTGAAGTAGGTAAGCTCTTTTTTTATTGGAAAGGCAATAGTCATGTGGCATGAAAGGTTTGTAAT
TATGGTAAAATCTATGTATG

Product: germination protein gerN

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MLFYFELVVILLCTKLAGDISVRLGQPSVLGKLIVGIIIGPAVLGIINSSELIDELSEIGVLLLMFMAGLETDLEELNRN
LKSSFAVAAGGIIFPFIGGYVTGLLFGLIQSHAIFLGLLLCATSVSITVQTLRDLGKMNTRESTTILGAAVFDDVIVVIL
LAFVMSFLGTQDVNITLVIVKKIIFFVSIVFIAWKVVPWIMKMLVPLRVTEALISAALIICFSFSYYSEMMGIAGIIGAF
AAGIAISQTEYKHEVEHKIEPIAYAIFVPVFFVSIGMEITFQGIGSQLWFIIIMTLIAIFTKLIGSGLGARLTGFNLQSS
ISIGAGMVSRGEVALIIAANGLAANLLAKENFTAIVIVVILTTIITPPLLKKYFV

Sequences:

>Translated_375_residues
MLFYFELVVILLCTKLAGDISVRLGQPSVLGKLIVGIIIGPAVLGIINSSELIDELSEIGVLLLMFMAGLETDLEELNRN
LKSSFAVAAGGIIFPFIGGYVTGLLFGLIQSHAIFLGLLLCATSVSITVQTLRDLGKMNTRESTTILGAAVFDDVIVVIL
LAFVMSFLGTQDVNITLVIVKKIIFFVSIVFIAWKVVPWIMKMLVPLRVTEALISAALIICFSFSYYSEMMGIAGIIGAF
AAGIAISQTEYKHEVEHKIEPIAYAIFVPVFFVSIGMEITFQGIGSQLWFIIIMTLIAIFTKLIGSGLGARLTGFNLQSS
ISIGAGMVSRGEVALIIAANGLAANLLAKENFTAIVIVVILTTIITPPLLKKYFV
>Mature_375_residues
MLFYFELVVILLCTKLAGDISVRLGQPSVLGKLIVGIIIGPAVLGIINSSELIDELSEIGVLLLMFMAGLETDLEELNRN
LKSSFAVAAGGIIFPFIGGYVTGLLFGLIQSHAIFLGLLLCATSVSITVQTLRDLGKMNTRESTTILGAAVFDDVIVVIL
LAFVMSFLGTQDVNITLVIVKKIIFFVSIVFIAWKVVPWIMKMLVPLRVTEALISAALIICFSFSYYSEMMGIAGIIGAF
AAGIAISQTEYKHEVEHKIEPIAYAIFVPVFFVSIGMEITFQGIGSQLWFIIIMTLIAIFTKLIGSGLGARLTGFNLQSS
ISIGAGMVSRGEVALIIAANGLAANLLAKENFTAIVIVVILTTIITPPLLKKYFV

Specific function: This is a Na(+)/H(+) antiporter. It can mediate sodium uptake when a transmembrane pH gradient is applied [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family [H]

Homologues:

Organism=Escherichia coli, GI1789749, Length=367, Percent_Identity=27.2479564032698, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1786232, Length=363, Percent_Identity=30.3030303030303, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1786685, Length=371, Percent_Identity=25.0673854447439, Blast_Score=97, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322367, Length=403, Percent_Identity=25.3101736972705, Blast_Score=111, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR004771 [H]

Pfam domain/function: PF00999 Na_H_Exchanger [H]

EC number: NA

Molecular weight: Translated: 40351; Mature: 40351

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFYFELVVILLCTKLAGDISVRLGQPSVLGKLIVGIIIGPAVLGIINSSELIDELSEIG
CHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VLLLMFMAGLETDLEELNRNLKSSFAVAAGGIIFPFIGGYVTGLLFGLIQSHAIFLGLLL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CATSVSITVQTLRDLGKMNTRESTTILGAAVFDDVIVVILLAFVMSFLGTQDVNITLVIV
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
KKIIFFVSIVFIAWKVVPWIMKMLVPLRVTEALISAALIICFSFSYYSEMMGIAGIIGAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAGIAISQTEYKHEVEHKIEPIAYAIFVPVFFVSIGMEITFQGIGSQLWFIIIMTLIAIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEEHHCCHHHHHHHHHHHHHHH
TKLIGSGLGARLTGFNLQSSISIGAGMVSRGEVALIIAANGLAANLLAKENFTAIVIVVI
HHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHCCCCHHHHHHHH
LTTIITPPLLKKYFV
HHHHHCHHHHHHHCC
>Mature Secondary Structure
MLFYFELVVILLCTKLAGDISVRLGQPSVLGKLIVGIIIGPAVLGIINSSELIDELSEIG
CHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VLLLMFMAGLETDLEELNRNLKSSFAVAAGGIIFPFIGGYVTGLLFGLIQSHAIFLGLLL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CATSVSITVQTLRDLGKMNTRESTTILGAAVFDDVIVVILLAFVMSFLGTQDVNITLVIV
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
KKIIFFVSIVFIAWKVVPWIMKMLVPLRVTEALISAALIICFSFSYYSEMMGIAGIIGAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAGIAISQTEYKHEVEHKIEPIAYAIFVPVFFVSIGMEITFQGIGSQLWFIIIMTLIAIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEEHHCCHHHHHHHHHHHHHHH
TKLIGSGLGARLTGFNLQSSISIGAGMVSRGEVALIIAANGLAANLLAKENFTAIVIVVI
HHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHCCCCHHHHHHHH
LTTIITPPLLKKYFV
HHHHHCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1312090; 1325937 [H]