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The map label for this gene is cwlD [H]

Identifier: 218901351

GI number: 218901351

Start: 140677

End: 141390

Strand: Direct

Name: cwlD [H]

Synonym: BCAH820_0159

Alternate gene names: 218901351

Gene position: 140677-141390 (Clockwise)

Preceding gene: 218901350

Following gene: 218901352

Centisome position: 2.65

GC content: 35.71

Gene sequence:

>714_bases
ATGAAGAGAATTCGAATTATCTCTTTTGCACTCGCTGCGGTTATTTTGTTTTTTTTAGTCAAACAAGAATTTCAAATTAC
AAAATCGTGGCGAGCTTGGAATTTACCCTTATCAGGGAAAGTAATTGTATTAGATGCTGGGCATGGTGGACCAGATGGAG
GAGCAGTTGGTGGCAAGGATATTGTAGAAAAAGATATTACGCTTGAGATTACAAAGAAGGTACAAGATTATTTACAAGAA
CAAGGTGCGTTAGTTATTTTAACGCGTGAGGGAGATTATGATTTAGCCCAGAAAGATACAAAGTCATATAGTAGACGTAA
AGCAGAAGATTTAAAAAAGCGTGTAGAGATTATTAATAAACCTGATGTAGACTTCTTTGCGAGCATTCATTTAAATGCTT
TGACTAGTAGTGGATCGAAGGGGGCACAAACGTTCTATTACCGTTCTTCAATTGAAAATGAACGTGCTGCGAAATTTATA
CAAGCTGAGTTGAGAACAAGTTTAGAGAATACAAATCGTTCTGCTAAAACAATTTCTCATGTGTATTTATTAAAGTATGC
GAAAACACCAGGCGCTTTAATTGAAGCTGGCTTTTTATCGAATGTAAATGAAAGGTATATGTTAAATTCGGAGAAATATC
AGCAAAAGGTAGCGGCTGCAGTATATCGTGGTATATTACGTTATTTCACGGAAAAAGGAAATCCTCCAGAATAA

Upstream 100 bases:

>100_bases
AGGTGAAAGAAGATATCGATTAAAGCTTCATTAAGACAAGCATAAACTTTCCTCTTGTCCCATATAAGTAATTAGAGGGA
CTAGCTGGAGGAGGAAAGGT

Downstream 100 bases:

>100_bases
GGGGGATTTTTTTGAGTTTCTCTCGTTAACGAAGTTTTCCGAAAATATGTTATACTGGAAATGTAAACGCATTTATTTCA
AAAGTGAAGAGAGATATATT

Product: germination-specific N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MKRIRIISFALAAVILFFLVKQEFQITKSWRAWNLPLSGKVIVLDAGHGGPDGGAVGGKDIVEKDITLEITKKVQDYLQE
QGALVILTREGDYDLAQKDTKSYSRRKAEDLKKRVEIINKPDVDFFASIHLNALTSSGSKGAQTFYYRSSIENERAAKFI
QAELRTSLENTNRSAKTISHVYLLKYAKTPGALIEAGFLSNVNERYMLNSEKYQQKVAAAVYRGILRYFTEKGNPPE

Sequences:

>Translated_237_residues
MKRIRIISFALAAVILFFLVKQEFQITKSWRAWNLPLSGKVIVLDAGHGGPDGGAVGGKDIVEKDITLEITKKVQDYLQE
QGALVILTREGDYDLAQKDTKSYSRRKAEDLKKRVEIINKPDVDFFASIHLNALTSSGSKGAQTFYYRSSIENERAAKFI
QAELRTSLENTNRSAKTISHVYLLKYAKTPGALIEAGFLSNVNERYMLNSEKYQQKVAAAVYRGILRYFTEKGNPPE
>Mature_237_residues
MKRIRIISFALAAVILFFLVKQEFQITKSWRAWNLPLSGKVIVLDAGHGGPDGGAVGGKDIVEKDITLEITKKVQDYLQE
QGALVILTREGDYDLAQKDTKSYSRRKAEDLKKRVEIINKPDVDFFASIHLNALTSSGSKGAQTFYYRSSIENERAAKFI
QAELRTSLENTNRSAKTISHVYLLKYAKTPGALIEAGFLSNVNERYMLNSEKYQQKVAAAVYRGILRYFTEKGNPPE

Specific function: Unknown

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=234, Percent_Identity=29.4871794871795, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1790611, Length=238, Percent_Identity=25.2100840336134, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI87082163, Length=235, Percent_Identity=26.3829787234043, Blast_Score=77, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR014234 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 26672; Mature: 26672

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIRIISFALAAVILFFLVKQEFQITKSWRAWNLPLSGKVIVLDAGHGGPDGGAVGGKD
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEECCCCCCCCCCCCCHH
IVEKDITLEITKKVQDYLQEQGALVILTREGDYDLAQKDTKSYSRRKAEDLKKRVEIINK
HHHCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
PDVDFFASIHLNALTSSGSKGAQTFYYRSSIENERAAKFIQAELRTSLENTNRSAKTISH
CCCHHHHHEEHHHHHCCCCCCCEEEEEHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHH
VYLLKYAKTPGALIEAGFLSNVNERYMLNSEKYQQKVAAAVYRGILRYFTEKGNPPE
HHHHHCCCCCCHHHHHHHHCCCCHHHEECCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKRIRIISFALAAVILFFLVKQEFQITKSWRAWNLPLSGKVIVLDAGHGGPDGGAVGGKD
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEECCCCCCCCCCCCCHH
IVEKDITLEITKKVQDYLQEQGALVILTREGDYDLAQKDTKSYSRRKAEDLKKRVEIINK
HHHCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
PDVDFFASIHLNALTSSGSKGAQTFYYRSSIENERAAKFIQAELRTSLENTNRSAKTISH
CCCHHHHHEEHHHHHCCCCCCCEEEEEHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHH
VYLLKYAKTPGALIEAGFLSNVNERYMLNSEKYQQKVAAAVYRGILRYFTEKGNPPE
HHHHHCCCCCCHHHHHHHHCCCCHHHEECCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7559346; 8969501; 9384377 [H]