Click here to switch to the map view.

The map label for this gene is eno [H]

Identifier: 218884751

GI number: 218884751

Start: 1332416

End: 1333723

Strand: Direct

Name: eno [H]

Synonym: DKAM_1440

Alternate gene names: 218884751

Gene position: 1332416-1333723 (Clockwise)

Preceding gene: 218884741

Following gene: 218884754

Centisome position: 97.6

GC content: 45.72

Gene sequence:

>1308_bases
ATGTATCTAGAAATATATGATGATGTATATGTAATAAAAGATGTAAAAGCCAGGATGATACTGGATAGCAGGGGTAATCC
AACAGTCCAGGTCAGAGTAGTAACAGAGGGTCTTGGAATAGGGATAGCTAACGCCCCCAGTGGTGCATCCACCGGCAAGC
ATGAAGCCGTGGAATTAAGGGATGGTGGAAAAGAGTTCAAGGGTAAAGGTGTCTCGAAAGCTGTGGAAAACGTTAACAAA
GTAATTGCCCCCACCATAATAGGCTTGAACTCAAGGCATCAATACGAGGTAGATGGTAAACTAATTGAGATAGATGGCAC
CCCTAATAAGGCCAGGCTTGGTGGAAACGCTATTGTGGCGACAAGCCTCGCTGTAGCCAAGGCTGCGGCATCAACCATGG
GTGTGCCCCTATACTACTATCTAGGTGGTAGGGCAGCGGAATTACTCCCGGTACCGCTTCTAAATATAATTAATGGTGGG
GTACACGCTGGGAACAAGCTAGATTTCCAAGAATTTATGATTGTTCCCGCCGGCTTCAACAGCTTCCACGACGCCATAAG
GGCGGCTGTCGAGGTATACCATGAATTAAAAATAGTCTTAAAGAACAAGTATGGTCCTTCAGCAGTTAATGTTGGTGATG
AAGGCGGGTATGCTCCTCCCCTCGAGAAGATACGTGATGCACTAGATCTCTTAGTTGAAGCGGTAAAGAAGGCTGGGTAT
GAGCCGGGTGGAGAGATAGCGATAGCACTTGATGCAGCCAGCTCTCAATTTTATAGAGAGGATAAGGGGGTATACGTCAT
TGAAGGAAGGGAGTTAGGTAGGAATGAAATGCTTGAATTATACGAGAAACTAGTAGGGGACTACCCTATAGTAAGTATAG
AGGACCCGTTATACGAGGAGGACTTCGAGGGATTCGCTGAAATGCGTAGAAGACTTGGGGATAAGATCCTGATAGTTGGA
GATGACTTATTTACAACAAACCCTGCCAGGCTCTCTAAGGGTATCGAGAGCAATGCTGGAAACGCCGTATTAGTTAAGGT
CAATCAGGTTGGAACCCTGAGCGAAACCATTGAAGTAGTTAGGATGGCACATGAAAACAGGTATAAGGCAATAATCAGTC
ATAGGAGTGGTGAAACAGAGGACACCAGTATAGCTGATATAGCTGTTGGATTATCCACTGGACTAATTAAGACTGGTGCA
CCGGGTAGAGGGGAGAGAACTGCTAAATATAATAGGCTACTAGAGATAGCCGAGGAATTGGAAAAGCCGAGGTATCCTGG
ATTCAGGGTGTTCCCGAGAAAACCATGA

Upstream 100 bases:

>100_bases
GGTGAATATACACCATAGTATATATGATGTAGTAATATTTACTAGAAGCAATAACCATAATAACTCCTCCATGTTAATTA
CCACCATGGGTGTGTCTGGA

Downstream 100 bases:

>100_bases
TTTTTTCACGGGATAAATATACCTATAATCATTAATACTATGGCCGTTATCACTGTTAGCTTGATAAACTGACTCACAGT
GAGGTTCGGTATCTCTTTTA

Product: enolase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MYLEIYDDVYVIKDVKARMILDSRGNPTVQVRVVTEGLGIGIANAPSGASTGKHEAVELRDGGKEFKGKGVSKAVENVNK
VIAPTIIGLNSRHQYEVDGKLIEIDGTPNKARLGGNAIVATSLAVAKAAASTMGVPLYYYLGGRAAELLPVPLLNIINGG
VHAGNKLDFQEFMIVPAGFNSFHDAIRAAVEVYHELKIVLKNKYGPSAVNVGDEGGYAPPLEKIRDALDLLVEAVKKAGY
EPGGEIAIALDAASSQFYREDKGVYVIEGRELGRNEMLELYEKLVGDYPIVSIEDPLYEEDFEGFAEMRRRLGDKILIVG
DDLFTTNPARLSKGIESNAGNAVLVKVNQVGTLSETIEVVRMAHENRYKAIISHRSGETEDTSIADIAVGLSTGLIKTGA
PGRGERTAKYNRLLEIAEELEKPRYPGFRVFPRKP

Sequences:

>Translated_435_residues
MYLEIYDDVYVIKDVKARMILDSRGNPTVQVRVVTEGLGIGIANAPSGASTGKHEAVELRDGGKEFKGKGVSKAVENVNK
VIAPTIIGLNSRHQYEVDGKLIEIDGTPNKARLGGNAIVATSLAVAKAAASTMGVPLYYYLGGRAAELLPVPLLNIINGG
VHAGNKLDFQEFMIVPAGFNSFHDAIRAAVEVYHELKIVLKNKYGPSAVNVGDEGGYAPPLEKIRDALDLLVEAVKKAGY
EPGGEIAIALDAASSQFYREDKGVYVIEGRELGRNEMLELYEKLVGDYPIVSIEDPLYEEDFEGFAEMRRRLGDKILIVG
DDLFTTNPARLSKGIESNAGNAVLVKVNQVGTLSETIEVVRMAHENRYKAIISHRSGETEDTSIADIAVGLSTGLIKTGA
PGRGERTAKYNRLLEIAEELEKPRYPGFRVFPRKP
>Mature_435_residues
MYLEIYDDVYVIKDVKARMILDSRGNPTVQVRVVTEGLGIGIANAPSGASTGKHEAVELRDGGKEFKGKGVSKAVENVNK
VIAPTIIGLNSRHQYEVDGKLIEIDGTPNKARLGGNAIVATSLAVAKAAASTMGVPLYYYLGGRAAELLPVPLLNIINGG
VHAGNKLDFQEFMIVPAGFNSFHDAIRAAVEVYHELKIVLKNKYGPSAVNVGDEGGYAPPLEKIRDALDLLVEAVKKAGY
EPGGEIAIALDAASSQFYREDKGVYVIEGRELGRNEMLELYEKLVGDYPIVSIEDPLYEEDFEGFAEMRRRLGDKILIVG
DDLFTTNPARLSKGIESNAGNAVLVKVNQVGTLSETIEVVRMAHENRYKAIISHRSGETEDTSIADIAVGLSTGLIKTGA
PGRGERTAKYNRLLEIAEELEKPRYPGFRVFPRKP

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the archaeal cel

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=434, Percent_Identity=50, Blast_Score=399, Evalue=1e-111,
Organism=Homo sapiens, GI4503571, Length=434, Percent_Identity=48.6175115207373, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI301897477, Length=422, Percent_Identity=49.2890995260664, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI301897469, Length=422, Percent_Identity=49.2890995260664, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI301897479, Length=419, Percent_Identity=45.1073985680191, Blast_Score=329, Evalue=3e-90,
Organism=Homo sapiens, GI169201331, Length=344, Percent_Identity=27.6162790697674, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI169201757, Length=344, Percent_Identity=27.6162790697674, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI239744207, Length=344, Percent_Identity=27.6162790697674, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI310129182, Length=134, Percent_Identity=31.3432835820896, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI310110045, Length=134, Percent_Identity=31.3432835820896, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI310120572, Length=134, Percent_Identity=31.3432835820896, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1789141, Length=421, Percent_Identity=52.0190023752969, Blast_Score=382, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17536383, Length=423, Percent_Identity=48.6997635933806, Blast_Score=383, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71995829, Length=423, Percent_Identity=48.6997635933806, Blast_Score=383, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI32563855, Length=185, Percent_Identity=43.2432432432432, Blast_Score=164, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6324974, Length=435, Percent_Identity=47.816091954023, Blast_Score=377, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6324969, Length=435, Percent_Identity=47.816091954023, Blast_Score=377, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6323985, Length=435, Percent_Identity=47.5862068965517, Blast_Score=375, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6321693, Length=425, Percent_Identity=49.6470588235294, Blast_Score=373, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6321968, Length=431, Percent_Identity=48.7238979118329, Blast_Score=351, Evalue=2e-97,
Organism=Drosophila melanogaster, GI24580918, Length=424, Percent_Identity=48.8207547169811, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580916, Length=424, Percent_Identity=48.8207547169811, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580920, Length=424, Percent_Identity=48.8207547169811, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580914, Length=424, Percent_Identity=48.8207547169811, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI281360527, Length=424, Percent_Identity=48.8207547169811, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI17137654, Length=424, Percent_Identity=48.8207547169811, Blast_Score=367, Evalue=1e-102,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 47212; Mature: 47212

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLEIYDDVYVIKDVKARMILDSRGNPTVQVRVVTEGLGIGIANAPSGASTGKHEAVELR
CEEEEECCEEEEECCCEEEEEECCCCCEEEEEEEECCCCEEECCCCCCCCCCCCCEEEEC
DGGKEFKGKGVSKAVENVNKVIAPTIIGLNSRHQYEVDGKLIEIDGTPNKARLGGNAIVA
CCCCHHCCCCHHHHHHHHHHHHHHHEEECCCCCEEEECCEEEEECCCCCCCEECCCEEEH
TSLAVAKAAASTMGVPLYYYLGGRAAELLPVPLLNIINGGVHAGNKLDFQEFMIVPAGFN
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCHHHEEEEECCCH
SFHDAIRAAVEVYHELKIVLKNKYGPSAVNVGDEGGYAPPLEKIRDALDLLVEAVKKAGY
HHHHHHHHHHHHHHHHHHEEECCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
EPGGEIAIALDAASSQFYREDKGVYVIEGRELGRNEMLELYEKLVGDYPIVSIEDPLYEE
CCCCCEEEEEECCCCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCHH
DFEGFAEMRRRLGDKILIVGDDLFTTNPARLSKGIESNAGNAVLVKVNQVGTLSETIEVV
HHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHH
RMAHENRYKAIISHRSGETEDTSIADIAVGLSTGLIKTGAPGRGERTAKYNRLLEIAEEL
HHHHCCHHEEEEECCCCCCCCCCHHHHEEHHHCCCEECCCCCCCCCHHHHHHHHHHHHHH
EKPRYPGFRVFPRKP
CCCCCCCEEECCCCC
>Mature Secondary Structure
MYLEIYDDVYVIKDVKARMILDSRGNPTVQVRVVTEGLGIGIANAPSGASTGKHEAVELR
CEEEEECCEEEEECCCEEEEEECCCCCEEEEEEEECCCCEEECCCCCCCCCCCCCEEEEC
DGGKEFKGKGVSKAVENVNKVIAPTIIGLNSRHQYEVDGKLIEIDGTPNKARLGGNAIVA
CCCCHHCCCCHHHHHHHHHHHHHHHEEECCCCCEEEECCEEEEECCCCCCCEECCCEEEH
TSLAVAKAAASTMGVPLYYYLGGRAAELLPVPLLNIINGGVHAGNKLDFQEFMIVPAGFN
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCHHHEEEEECCCH
SFHDAIRAAVEVYHELKIVLKNKYGPSAVNVGDEGGYAPPLEKIRDALDLLVEAVKKAGY
HHHHHHHHHHHHHHHHHHEEECCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
EPGGEIAIALDAASSQFYREDKGVYVIEGRELGRNEMLELYEKLVGDYPIVSIEDPLYEE
CCCCCEEEEEECCCCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCHH
DFEGFAEMRRRLGDKILIVGDDLFTTNPARLSKGIESNAGNAVLVKVNQVGTLSETIEVV
HHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHH
RMAHENRYKAIISHRSGETEDTSIADIAVGLSTGLIKTGAPGRGERTAKYNRLLEIAEEL
HHHHCCHHEEEEECCCCCCCCCCHHHHEEHHHCCCEECCCCCCCCCHHHHHHHHHHHHHH
EKPRYPGFRVFPRKP
CCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA