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The map label for this gene is thrS [H]

Identifier: 218884527

GI number: 218884527

Start: 1137818

End: 1139662

Strand: Direct

Name: thrS [H]

Synonym: DKAM_1216

Alternate gene names: 218884527

Gene position: 1137818-1139662 (Clockwise)

Preceding gene: 218884525

Following gene: 218884531

Centisome position: 83.34

GC content: 46.18

Gene sequence:

>1845_bases
GTGTCCACATTGAGGATACTACTTATTCATGCACGCAGATTTAGCTATAGTGTTGTTAAGCCAGCTGTAGAGGAACCAGA
GCCCTTGGAAGAAGGCGGAAAGACCCTCATGCTTGAAAACGTGTTGGTGGTCTTCACCAGTATTGAGAGAGGTGATGATG
AATCCATAATATCCAGTGCCGTCAACGAGGTGATCAACGTATTCAACCAGGTTAAGGCATCCTCAATACTACTATACCCC
TACGCACATCTCTCACCGGATCTCGCCCCACCATTCGAGGCTGTAAAAATACTCAACGCCTTCTACACCGCGTTGAAGAA
CACCGGTTTACAAGTATATAAGGCTCCGTTTGGATGGTATAAGTCTTTTACTCTTGAATGCTATGGACACCCGTTAAGCG
AGCTCTCAAAGACCATTAAGAAGTCCGAGACCGTTGCGAAGAGAATTATCGAGAAGAAGTTCTACATTATGACTCCTGAT
GGCAGCTTGCATGATCCAACAAGCTTCGACTACACCAACTACCCGGAGTTAAAGATCCTCGTAGATAAGGAGGTATTCGG
CAAAGAGCTCACCGGTGGCGAGAATAGGGTAAACGAGTACTGTAGGAAGTTCGGGTTCGAATGGGAGCCAATGAGTGACC
ATGGACACATGAGGTATGGACCCCATGCAACTGTAATAATGGAGGCTGTAATGAGGTACTCATGGATGGTTGCCCAGAGC
CTAGGTATACCGGTCTTCAAAGTCATGGGTAGCAATATGTTTAACCTTAAGGAGAAACCCGTATATGAGCACGCTGTCCT
ATTCGGTGATAGATTATATGAGGTCCAGTTGGATGAAGACCGCTTTATTCTCAGGTATGCTGCATGCCATCAACAGTTCG
CGATGCTCAGGGACTGGGTTATAAGCTATAAGGACCTCCCCTTCGGCGTGTTCGAGGTAGCCGATAGTTACAGGCTTGAG
CAACGCGGTGAACTAGCCCTATGTTTCAGGCTGAGAAAGTTCTACATGCCAGACCTCCATATCTTAACCAGGAACATAGG
TGAAGCGATCGACGTATCTATGAGGGTTCAAGCCAAGATACATGAGGAGGCTGGGAAGGCTGGGAGAAGCTATGTGGCAC
TATACAATATAACCGATGAATTCCTGGAGAAGTACAGAGATAAACTAGTGGAGTTCGTTAAGAGGGAGAAATATCCCGTC
CTCCTCGCGGTGATACCATCAGGGATATATTACTGGGTACTCAACGTTGAATACCATATAATAGACAACCTGAAGAGACC
GAGGGAGATAGCAACCTTCCAGATAGACGTAGGGAACGGTAGGAGGTTTAATATAGCCTACGTAGATGAGAAGGGAGAGA
AACACCATCCAGTCATAATACATACCGCATTGCTGGGTGGTGTTGAGAGATATATCTACATGGTATTGGATACCGGGGCA
TTAATAGAGGCAAGTGGTCAAACCCCGTACATACCAACCTGGATGAGTCCGGTCCAAGTAAGAATAGTACCTGTTTCAAA
AGAATATAATGAACACGCCTTCAAAATAGCCGGCGAGATACTCTCACATGGGTTCAGGGTAGATGTCGACGACAGGGATG
AAACCCTTGGGAAAAAGATACGGGATGCCGGGAAAGAATGGATACCATACATAATAGTGGTAGGCGAGAGAGAAGTTAAT
ACTGGAACCATCAACATCAGGGTAAGGAGAAGCAATGATCAACGGGTCATGAAGTTGGAGGAATTCATAAAGTTACTCGA
GGAAGAAGTCAAGGGGTATCCCAGGGTAGAAATGACCCTCCCAGTTAGCGTTGCATCCAGACCCCTATTCTCGTATAAAC
AATAG

Upstream 100 bases:

>100_bases
GCCTAATATAGGATAACGTGGTCACGTAACCTTTAGTATATGGTAAATCAATATATCAATACATATTTTAATATCCCCCT
AGATAACACTTAGATAAAAG

Downstream 100 bases:

>100_bases
TTTTTCCGCATTCTATTCCTCAACGTAAACAAGTATCTCGTGGAAAGTATTCCTCCCACAGTTAGGGCAGTAGTGGTATA
ACTGCTTAAACTCCTTCTTC

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 614; Mature: 613

Protein sequence:

>614_residues
MSTLRILLIHARRFSYSVVKPAVEEPEPLEEGGKTLMLENVLVVFTSIERGDDESIISSAVNEVINVFNQVKASSILLYP
YAHLSPDLAPPFEAVKILNAFYTALKNTGLQVYKAPFGWYKSFTLECYGHPLSELSKTIKKSETVAKRIIEKKFYIMTPD
GSLHDPTSFDYTNYPELKILVDKEVFGKELTGGENRVNEYCRKFGFEWEPMSDHGHMRYGPHATVIMEAVMRYSWMVAQS
LGIPVFKVMGSNMFNLKEKPVYEHAVLFGDRLYEVQLDEDRFILRYAACHQQFAMLRDWVISYKDLPFGVFEVADSYRLE
QRGELALCFRLRKFYMPDLHILTRNIGEAIDVSMRVQAKIHEEAGKAGRSYVALYNITDEFLEKYRDKLVEFVKREKYPV
LLAVIPSGIYYWVLNVEYHIIDNLKRPREIATFQIDVGNGRRFNIAYVDEKGEKHHPVIIHTALLGGVERYIYMVLDTGA
LIEASGQTPYIPTWMSPVQVRIVPVSKEYNEHAFKIAGEILSHGFRVDVDDRDETLGKKIRDAGKEWIPYIIVVGEREVN
TGTINIRVRRSNDQRVMKLEEFIKLLEEEVKGYPRVEMTLPVSVASRPLFSYKQ

Sequences:

>Translated_614_residues
MSTLRILLIHARRFSYSVVKPAVEEPEPLEEGGKTLMLENVLVVFTSIERGDDESIISSAVNEVINVFNQVKASSILLYP
YAHLSPDLAPPFEAVKILNAFYTALKNTGLQVYKAPFGWYKSFTLECYGHPLSELSKTIKKSETVAKRIIEKKFYIMTPD
GSLHDPTSFDYTNYPELKILVDKEVFGKELTGGENRVNEYCRKFGFEWEPMSDHGHMRYGPHATVIMEAVMRYSWMVAQS
LGIPVFKVMGSNMFNLKEKPVYEHAVLFGDRLYEVQLDEDRFILRYAACHQQFAMLRDWVISYKDLPFGVFEVADSYRLE
QRGELALCFRLRKFYMPDLHILTRNIGEAIDVSMRVQAKIHEEAGKAGRSYVALYNITDEFLEKYRDKLVEFVKREKYPV
LLAVIPSGIYYWVLNVEYHIIDNLKRPREIATFQIDVGNGRRFNIAYVDEKGEKHHPVIIHTALLGGVERYIYMVLDTGA
LIEASGQTPYIPTWMSPVQVRIVPVSKEYNEHAFKIAGEILSHGFRVDVDDRDETLGKKIRDAGKEWIPYIIVVGEREVN
TGTINIRVRRSNDQRVMKLEEFIKLLEEEVKGYPRVEMTLPVSVASRPLFSYKQ
>Mature_613_residues
STLRILLIHARRFSYSVVKPAVEEPEPLEEGGKTLMLENVLVVFTSIERGDDESIISSAVNEVINVFNQVKASSILLYPY
AHLSPDLAPPFEAVKILNAFYTALKNTGLQVYKAPFGWYKSFTLECYGHPLSELSKTIKKSETVAKRIIEKKFYIMTPDG
SLHDPTSFDYTNYPELKILVDKEVFGKELTGGENRVNEYCRKFGFEWEPMSDHGHMRYGPHATVIMEAVMRYSWMVAQSL
GIPVFKVMGSNMFNLKEKPVYEHAVLFGDRLYEVQLDEDRFILRYAACHQQFAMLRDWVISYKDLPFGVFEVADSYRLEQ
RGELALCFRLRKFYMPDLHILTRNIGEAIDVSMRVQAKIHEEAGKAGRSYVALYNITDEFLEKYRDKLVEFVKREKYPVL
LAVIPSGIYYWVLNVEYHIIDNLKRPREIATFQIDVGNGRRFNIAYVDEKGEKHHPVIIHTALLGGVERYIYMVLDTGAL
IEASGQTPYIPTWMSPVQVRIVPVSKEYNEHAFKIAGEILSHGFRVDVDDRDETLGKKIRDAGKEWIPYIIVVGEREVNT
GTINIRVRRSNDQRVMKLEEFIKLLEEEVKGYPRVEMTLPVSVASRPLFSYKQ

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38570111, Length=413, Percent_Identity=26.1501210653753, Blast_Score=126, Evalue=7e-29,
Organism=Homo sapiens, GI38202255, Length=432, Percent_Identity=26.6203703703704, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI20070344, Length=318, Percent_Identity=28.6163522012579, Blast_Score=103, Evalue=4e-22,
Organism=Escherichia coli, GI1788013, Length=332, Percent_Identity=26.5060240963855, Blast_Score=130, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71984184, Length=351, Percent_Identity=27.9202279202279, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI71984192, Length=351, Percent_Identity=27.9202279202279, Blast_Score=126, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6322112, Length=363, Percent_Identity=26.7217630853994, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6322654, Length=368, Percent_Identity=24.1847826086957, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24583839, Length=349, Percent_Identity=26.3610315186246, Blast_Score=129, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24583842, Length=398, Percent_Identity=25.8793969849246, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI28574141, Length=398, Percent_Identity=25.8793969849246, Blast_Score=128, Evalue=1e-29,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002320
- InterPro:   IPR015011
- ProDom:   PD016189 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF08915 tRNA-Thr_ED [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 70844; Mature: 70712

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLRILLIHARRFSYSVVKPAVEEPEPLEEGGKTLMLENVLVVFTSIERGDDESIISSA
CCCEEEEEEEHHHCCCHHHCCCCCCCCCHHHCCCEEEECCEEEEEEECCCCCCHHHHHHH
VNEVINVFNQVKASSILLYPYAHLSPDLAPPFEAVKILNAFYTALKNTGLQVYKAPFGWY
HHHHHHHHHHHCCCEEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
KSFTLECYGHPLSELSKTIKKSETVAKRIIEKKFYIMTPDGSLHDPTSFDYTNYPELKIL
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCEEEE
VDKEVFGKELTGGENRVNEYCRKFGFEWEPMSDHGHMRYGPHATVIMEAVMRYSWMVAQS
EEHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHH
LGIPVFKVMGSNMFNLKEKPVYEHAVLFGDRLYEVQLDEDRFILRYAACHQQFAMLRDWV
CCCCHHHHHCCCCCCCCCCCCHHHHHHCCCEEEEEEECCCCEEHHHHHHHHHHHHHHHHH
ISYKDLPFGVFEVADSYRLEQRGELALCFRLRKFYMPDLHILTRNIGEAIDVSMRVQAKI
HHHCCCCCHHHHHHHHHCHHCCCCCHHHHHHHHHCCCCHHHHHHHHCHHHCEEEEEHHHH
HEEAGKAGRSYVALYNITDEFLEKYRDKLVEFVKREKYPVLLAVIPSGIYYWVLNVEYHI
HHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEEEEEEE
IDNLKRPREIATFQIDVGNGRRFNIAYVDEKGEKHHPVIIHTALLGGVERYIYMVLDTGA
HHCCCCCCEEEEEEEECCCCCEEEEEEEECCCCCCCCEEEEHHHHHHHHHEEEEEEECCC
LIEASGQTPYIPTWMSPVQVRIVPVSKEYNEHAFKIAGEILSHGFRVDVDDRDETLGKKI
EEEECCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHHHH
RDAGKEWIPYIIVVGEREVNTGTINIRVRRSNDQRVMKLEEFIKLLEEEVKGYPRVEMTL
HHCCCCCCCEEEEECCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
PVSVASRPLFSYKQ
EHHHHCCCCCCCCC
>Mature Secondary Structure 
STLRILLIHARRFSYSVVKPAVEEPEPLEEGGKTLMLENVLVVFTSIERGDDESIISSA
CCEEEEEEEHHHCCCHHHCCCCCCCCCHHHCCCEEEECCEEEEEEECCCCCCHHHHHHH
VNEVINVFNQVKASSILLYPYAHLSPDLAPPFEAVKILNAFYTALKNTGLQVYKAPFGWY
HHHHHHHHHHHCCCEEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
KSFTLECYGHPLSELSKTIKKSETVAKRIIEKKFYIMTPDGSLHDPTSFDYTNYPELKIL
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCEEEE
VDKEVFGKELTGGENRVNEYCRKFGFEWEPMSDHGHMRYGPHATVIMEAVMRYSWMVAQS
EEHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHH
LGIPVFKVMGSNMFNLKEKPVYEHAVLFGDRLYEVQLDEDRFILRYAACHQQFAMLRDWV
CCCCHHHHHCCCCCCCCCCCCHHHHHHCCCEEEEEEECCCCEEHHHHHHHHHHHHHHHHH
ISYKDLPFGVFEVADSYRLEQRGELALCFRLRKFYMPDLHILTRNIGEAIDVSMRVQAKI
HHHCCCCCHHHHHHHHHCHHCCCCCHHHHHHHHHCCCCHHHHHHHHCHHHCEEEEEHHHH
HEEAGKAGRSYVALYNITDEFLEKYRDKLVEFVKREKYPVLLAVIPSGIYYWVLNVEYHI
HHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEEEEEEE
IDNLKRPREIATFQIDVGNGRRFNIAYVDEKGEKHHPVIIHTALLGGVERYIYMVLDTGA
HHCCCCCCEEEEEEEECCCCCEEEEEEEECCCCCCCCEEEEHHHHHHHHHEEEEEEECCC
LIEASGQTPYIPTWMSPVQVRIVPVSKEYNEHAFKIAGEILSHGFRVDVDDRDETLGKKI
EEEECCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHHHH
RDAGKEWIPYIIVVGEREVNTGTINIRVRRSNDQRVMKLEEFIKLLEEEVKGYPRVEMTL
HHCCCCCCCEEEEECCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
PVSVASRPLFSYKQ
EHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA