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The map label for this gene is metG [H]

Identifier: 218884158

GI number: 218884158

Start: 815168

End: 815494

Strand: Reverse

Name: metG [H]

Synonym: DKAM_0847

Alternate gene names: 218884158

Gene position: 815494-815168 (Counterclockwise)

Preceding gene: 218884159

Following gene: 218884154

Centisome position: 59.73

GC content: 43.12

Gene sequence:

>327_bases
ATGGAATTAATTGGAATAGATGAGTTCCAGAAGATTGATTTAAGGGTTGGACTGGTTAAATCAGCCGAGAAAGTCCCTGG
TAGCGAGAAGTTGATCAGGCTCATAGTAGACCTGGGGGAACTTGGCGAGAGGCAGATTATAGCTGGACTTGGTAAGTGGT
ATCAGCCAGAGTTTTTCCAGGGCAAATATGTTATCATAGTGGCTAATTTGAAACCTAAGAAAATGATGGGTTTTGAGAGT
CAGGGAATGCTTCTAGCAACGGATACTGATCCACCTGTTATAGCTACTGTTGAAAAAACAGTTAAGCCTGGGGCGAGGCT
TTTCTAG

Upstream 100 bases:

>100_bases
CGGTATAAGGGTTCATGAGAAAAGCCCTATCGAGGTCTTTGAAGAGTTATCAAGTAATTTAAGGGATAACTATAATTAAT
AACTAATCCAGGTGGCTCCC

Downstream 100 bases:

>100_bases
CCTCCAGGTATCTTCTATACCATATCATTTTTGAAAGCGCGTAAACCGCTCTCTCAGGCGATGGATAAGCGGGTATATCA
TGTCTGTTCAGGTACTTTAT

Product: methionyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 108; Mature: 108

Protein sequence:

>108_residues
MELIGIDEFQKIDLRVGLVKSAEKVPGSEKLIRLIVDLGELGERQIIAGLGKWYQPEFFQGKYVIIVANLKPKKMMGFES
QGMLLATDTDPPVIATVEKTVKPGARLF

Sequences:

>Translated_108_residues
MELIGIDEFQKIDLRVGLVKSAEKVPGSEKLIRLIVDLGELGERQIIAGLGKWYQPEFFQGKYVIIVANLKPKKMMGFES
QGMLLATDTDPPVIATVEKTVKPGARLF
>Mature_108_residues
MELIGIDEFQKIDLRVGLVKSAEKVPGSEKLIRLIVDLGELGERQIIAGLGKWYQPEFFQGKYVIIVANLKPKKMMGFES
QGMLLATDTDPPVIATVEKTVKPGARLF

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0073

COG function: function code R; EMAP domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI4507947, Length=94, Percent_Identity=36.1702127659575, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788432, Length=87, Percent_Identity=47.1264367816092, Blast_Score=74, Evalue=2e-15,
Organism=Escherichia coli, GI1789455, Length=110, Percent_Identity=30.9090909090909, Blast_Score=59, Evalue=7e-11,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 12002; Mature: 12002

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELIGIDEFQKIDLRVGLVKSAEKVPGSEKLIRLIVDLGELGERQIIAGLGKWYQPEFFQ
CCCCCCCCCEEEEEEEEEECCHHHCCCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCC
GKYVIIVANLKPKKMMGFESQGMLLATDTDPPVIATVEKTVKPGARLF
CEEEEEEECCCCHHHCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure
MELIGIDEFQKIDLRVGLVKSAEKVPGSEKLIRLIVDLGELGERQIIAGLGKWYQPEFFQ
CCCCCCCCCEEEEEEEEEECCHHHCCCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCC
GKYVIIVANLKPKKMMGFESQGMLLATDTDPPVIATVEKTVKPGARLF
CEEEEEEECCCCHHHCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA