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The map label for this gene is 218883852

Identifier: 218883852

GI number: 218883852

Start: 507293

End: 508375

Strand: Direct

Name: 218883852

Synonym: DKAM_0541

Alternate gene names: NA

Gene position: 507293-508375 (Clockwise)

Preceding gene: 218883843

Following gene: 218883853

Centisome position: 37.16

GC content: 49.95

Gene sequence:

>1083_bases
ATGAGAAGCTTGAAGTTCTCCCCCGACCTACCATGGGTTAGGGAAGCAGATTTCTGGGAGCCCCTTGGAGACGGAAGGGT
ACGCTGTAACCTTTGCCATAGGAGATGCATCATAGTGCCGGGAGCCTATGGGGCATGCGGTGTTAGATATAATCATGAAG
GCAGGCTCTACACGCTGGTGTATGGGCTGCTTACAGCGGCTAACCCAGACCCCATAGAGAAGAAGCCCCTAATGCACTTC
AACCCCGGTGCATGCGCATTCTCTATTTCAACAGTCGGATGCAACTTCTACTGTAGATTCTGCCAGAACTGGGTGCTCAG
CCAGTCAAGGAGGAATGGTGCCTTCGGGAAGCCATATGAACCCCGGGAAGTGGTTGAAGAGGCCTTAGGTAACGGCTGTG
ATGGAATAAGCTACACATATAATGAGCCAACAATATTCTACGAGTTCATGTACGATGTTGCAAGGCTCGCCAAGGAGAAG
GACTTGTTCAACACGATGGTCACAAACGGCTATATGACACCTGAGGCCATTAGGAAGCTCGGTAAACTCATGGATGCGGC
AACCGTTGACTTCAAGGCCTCAGGTAATAAGGAGTTCTATAGGAGGTACATGGGTGTACTGGATCCCTCACCAATATACG
AGAGCCTCATCGAGATGAAGAGACAGGGTTGGTGGATCGAAGTGACGAGCCTCATTGTTCCAGGTGTCGGCGATAGAATA
GAGGATACGGCGGGCCTTGCTAAATGGATTGCCGAGAACCTCGGAGAGGAGACGCCATTCCACCTCCTGAGATTTCACCC
AGACTACCTGGCGCAGGATATCCCTCCAACACCTGTTAAGACGTTAGAGAGGCATGCTGAAGCGGCTAGAGAGGCGGGAT
TAAAGCATGTTTACATAGGTAATGTGTGGGGGCATCCCTTAGAGAATACCTATTGTCCTAGATGCGGCTCCCTTGTTGTG
GAGAGAAACGGCTTCTATATTATCGATTGGAGGCTCGGAGAGGATTTTAAATGCCCTAAATGCGGTTATAGATTGAACTT
TGCAGGAAGATTCCGCGGTAATAAGGGATACATGTATTGGTGA

Upstream 100 bases:

>100_bases
TATATTGAATCCATCAGGGTAGTTACCGGTTACAACAGCCTTAATCAACACGAGTCACCAGTATATTATAATACGGGTCT
AAACAATTTTATTTAAGAGG

Downstream 100 bases:

>100_bases
CCCTAGATGCCGTACTTCGTGGTGGTGTTGGGAACCGCCGGAAGCGGTAAGACCTCGTTAACTAGTGCATTATACACGTA
TCTAACATCACACCAGCTTG

Product: Pyruvate-formate lyase-activating enzyme

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MRSLKFSPDLPWVREADFWEPLGDGRVRCNLCHRRCIIVPGAYGACGVRYNHEGRLYTLVYGLLTAANPDPIEKKPLMHF
NPGACAFSISTVGCNFYCRFCQNWVLSQSRRNGAFGKPYEPREVVEEALGNGCDGISYTYNEPTIFYEFMYDVARLAKEK
DLFNTMVTNGYMTPEAIRKLGKLMDAATVDFKASGNKEFYRRYMGVLDPSPIYESLIEMKRQGWWIEVTSLIVPGVGDRI
EDTAGLAKWIAENLGEETPFHLLRFHPDYLAQDIPPTPVKTLERHAEAAREAGLKHVYIGNVWGHPLENTYCPRCGSLVV
ERNGFYIIDWRLGEDFKCPKCGYRLNFAGRFRGNKGYMYW

Sequences:

>Translated_360_residues
MRSLKFSPDLPWVREADFWEPLGDGRVRCNLCHRRCIIVPGAYGACGVRYNHEGRLYTLVYGLLTAANPDPIEKKPLMHF
NPGACAFSISTVGCNFYCRFCQNWVLSQSRRNGAFGKPYEPREVVEEALGNGCDGISYTYNEPTIFYEFMYDVARLAKEK
DLFNTMVTNGYMTPEAIRKLGKLMDAATVDFKASGNKEFYRRYMGVLDPSPIYESLIEMKRQGWWIEVTSLIVPGVGDRI
EDTAGLAKWIAENLGEETPFHLLRFHPDYLAQDIPPTPVKTLERHAEAAREAGLKHVYIGNVWGHPLENTYCPRCGSLVV
ERNGFYIIDWRLGEDFKCPKCGYRLNFAGRFRGNKGYMYW
>Mature_360_residues
MRSLKFSPDLPWVREADFWEPLGDGRVRCNLCHRRCIIVPGAYGACGVRYNHEGRLYTLVYGLLTAANPDPIEKKPLMHF
NPGACAFSISTVGCNFYCRFCQNWVLSQSRRNGAFGKPYEPREVVEEALGNGCDGISYTYNEPTIFYEFMYDVARLAKEK
DLFNTMVTNGYMTPEAIRKLGKLMDAATVDFKASGNKEFYRRYMGVLDPSPIYESLIEMKRQGWWIEVTSLIVPGVGDRI
EDTAGLAKWIAENLGEETPFHLLRFHPDYLAQDIPPTPVKTLERHAEAAREAGLKHVYIGNVWGHPLENTYCPRCGSLVV
ERNGFYIIDWRLGEDFKCPKCGYRLNFAGRFRGNKGYMYW

Specific function: Unknown

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR016431
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 41221; Mature: 41221

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSLKFSPDLPWVREADFWEPLGDGRVRCNLCHRRCIIVPGAYGACGVRYNHEGRLYTLV
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCEEEEECCCCCCCCEEECCCCCCHHHH
YGLLTAANPDPIEKKPLMHFNPGACAFSISTVGCNFYCRFCQNWVLSQSRRNGAFGKPYE
HHHHHCCCCCCCCCCCCCEECCCCEEEEEEHHCHHHHHHHHHHHHHHHHCCCCCCCCCCC
PREVVEEALGNGCDGISYTYNEPTIFYEFMYDVARLAKEKDLFNTMVTNGYMTPEAIRKL
HHHHHHHHHCCCCCCCCEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
GKLMDAATVDFKASGNKEFYRRYMGVLDPSPIYESLIEMKRQGWWIEVTSLIVPGVGDRI
HHHHHHHEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCHH
EDTAGLAKWIAENLGEETPFHLLRFHPDYLAQDIPPTPVKTLERHAEAAREAGLKHVYIG
HHHHHHHHHHHHHCCCCCCHHHEEECHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEC
NVWGHPLENTYCPRCGSLVVERNGFYIIDWRLGEDFKCPKCGYRLNFAGRFRGNKGYMYW
CCCCCCCCCCCCCCCCCEEEECCCEEEEEEEECCCCCCCCCCEEEEEEEEEECCCCCCCC
>Mature Secondary Structure
MRSLKFSPDLPWVREADFWEPLGDGRVRCNLCHRRCIIVPGAYGACGVRYNHEGRLYTLV
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCEEEEECCCCCCCCEEECCCCCCHHHH
YGLLTAANPDPIEKKPLMHFNPGACAFSISTVGCNFYCRFCQNWVLSQSRRNGAFGKPYE
HHHHHCCCCCCCCCCCCCEECCCCEEEEEEHHCHHHHHHHHHHHHHHHHCCCCCCCCCCC
PREVVEEALGNGCDGISYTYNEPTIFYEFMYDVARLAKEKDLFNTMVTNGYMTPEAIRKL
HHHHHHHHHCCCCCCCCEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
GKLMDAATVDFKASGNKEFYRRYMGVLDPSPIYESLIEMKRQGWWIEVTSLIVPGVGDRI
HHHHHHHEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCHH
EDTAGLAKWIAENLGEETPFHLLRFHPDYLAQDIPPTPVKTLERHAEAAREAGLKHVYIG
HHHHHHHHHHHHHCCCCCCHHHEEECHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEC
NVWGHPLENTYCPRCGSLVVERNGFYIIDWRLGEDFKCPKCGYRLNFAGRFRGNKGYMYW
CCCCCCCCCCCCCCCCCEEEECCCEEEEEEEECCCCCCCCCCEEEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]