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The map label for this gene is purC [H]

Identifier: 218883830

GI number: 218883830

Start: 485908

End: 486669

Strand: Direct

Name: purC [H]

Synonym: DKAM_0519

Alternate gene names: 218883830

Gene position: 485908-486669 (Clockwise)

Preceding gene: 218883826

Following gene: 218883831

Centisome position: 35.59

GC content: 42.78

Gene sequence:

>762_bases
GTGGTGTCCTCAAGTATTAACGTGGTGGACATGGAGCTTAAATTATTATATGAAGGAAAAACTAAGAGGGTCTACTCTAA
AACACCCGAGTTACTCGTATTGGAGTTCAAGGATGATGTTACAGCGATGGATGGATTGGTTAAGAAAACCATGCCCGGTA
AAGGCGTAATCAATGCGAGGCTCTCCGCATATTTATTTAAGCACATTGAGAAACAGGGTATTGAGACGCATCTCGTAGAA
TATAATGGAGACCGCTTCCTTACAGTTAGAAGGCTTGAGATGCTCCCTATAGAAGTGATCGTCAGGAATTATGCATATGG
TTCCCTCCTTAAGAGAATGCCCCTCTATAAGAAGATGCAGGAGCTCAATCCACCACTAGTGGAGTTCCACTATAAGGATG
ACTCACTCCATGATCCGTTGATCACACCAGAGGATATTACCTACACAGGTCTGTTAACTAGTGGTGAATTAAGGTGGTTA
ATCGAGGCAACGATAAAAATAAACAATGTATTAAGCGAATTATTTAGTGGAGCAGGGCTTAAACTAGTAGACTTCAAGGT
AGAGTATGGAAGAAATAGAAATGGGAGACTAATACTAGGCGACGAGCTCACAGGTGACTCGATAAGGGTTTTAGACGATA
AGGGGAACCACCTTGATAAAGAGGTGTTCAGGAAAACCGGTGACATAGAAGCCTTGAGAACAGCCTATATTGAGCTATGC
AACAGGCTCGGGGTCAATGTCGGCGATGTTGTTCACGGTTAA

Upstream 100 bases:

>100_bases
GGGCGTCAATGATACTGGTTAACATTAATGAAGGTAATACTGGGGCCGGTATCGTTTTACATGGTTTCATTAAATCAATT
AAAACAATTGTTATCAAAGC

Downstream 100 bases:

>100_bases
CGTGGTGATATCGTATAAGCCCTCCATTAGGGATCCCGAGGGAGAGACCATATATAGACAATTAATACTGAAGAACAATT
ACAGCATGATGAAGAATGTT

Product: phosphoribosylaminoimidazole-succinocarboxamide synthase

Products: NA

Alternate protein names: SAICAR synthetase [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MVSSSINVVDMELKLLYEGKTKRVYSKTPELLVLEFKDDVTAMDGLVKKTMPGKGVINARLSAYLFKHIEKQGIETHLVE
YNGDRFLTVRRLEMLPIEVIVRNYAYGSLLKRMPLYKKMQELNPPLVEFHYKDDSLHDPLITPEDITYTGLLTSGELRWL
IEATIKINNVLSELFSGAGLKLVDFKVEYGRNRNGRLILGDELTGDSIRVLDDKGNHLDKEVFRKTGDIEALRTAYIELC
NRLGVNVGDVVHG

Sequences:

>Translated_253_residues
MVSSSINVVDMELKLLYEGKTKRVYSKTPELLVLEFKDDVTAMDGLVKKTMPGKGVINARLSAYLFKHIEKQGIETHLVE
YNGDRFLTVRRLEMLPIEVIVRNYAYGSLLKRMPLYKKMQELNPPLVEFHYKDDSLHDPLITPEDITYTGLLTSGELRWL
IEATIKINNVLSELFSGAGLKLVDFKVEYGRNRNGRLILGDELTGDSIRVLDDKGNHLDKEVFRKTGDIEALRTAYIELC
NRLGVNVGDVVHG
>Mature_253_residues
MVSSSINVVDMELKLLYEGKTKRVYSKTPELLVLEFKDDVTAMDGLVKKTMPGKGVINARLSAYLFKHIEKQGIETHLVE
YNGDRFLTVRRLEMLPIEVIVRNYAYGSLLKRMPLYKKMQELNPPLVEFHYKDDSLHDPLITPEDITYTGLLTSGELRWL
IEATIKINNVLSELFSGAGLKLVDFKVEYGRNRNGRLILGDELTGDSIRVLDDKGNHLDKEVFRKTGDIEALRTAYIELC
NRLGVNVGDVVHG

Specific function: De novo purine biosynthesis; seventh step. [C]

COG id: COG0152

COG function: function code F; Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SAICAR synthetase family [H]

Homologues:

Organism=Homo sapiens, GI119220557, Length=222, Percent_Identity=32.4324324324324, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI5453539, Length=222, Percent_Identity=32.4324324324324, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI119220559, Length=229, Percent_Identity=31.0043668122271, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI1788820, Length=234, Percent_Identity=38.4615384615385, Blast_Score=155, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17531275, Length=222, Percent_Identity=30.6306306306306, Blast_Score=104, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24583917, Length=220, Percent_Identity=33.1818181818182, Blast_Score=117, Evalue=7e-27,
Organism=Drosophila melanogaster, GI18860083, Length=228, Percent_Identity=31.5789473684211, Blast_Score=114, Evalue=5e-26,

Paralogues:

None

Copy number: 1680 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 13,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013816
- InterPro:   IPR001636
- InterPro:   IPR018236 [H]

Pfam domain/function: PF01259 SAICAR_synt [H]

EC number: =6.3.2.6 [H]

Molecular weight: Translated: 28757; Mature: 28757

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS01057 SAICAR_SYNTHETASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSSSINVVDMELKLLYEGKTKRVYSKTPELLVLEFKDDVTAMDGLVKKTMPGKGVINAR
CCCCCCEEEEEEEEEEECCCCCHHHCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCHHHH
LSAYLFKHIEKQGIETHLVEYNGDRFLTVRRLEMLPIEVIVRNYAYGSLLKRMPLYKKMQ
HHHHHHHHHHHCCCEEEEEEECCCEEEEEEHHHHCHHHHHHHHHHHHHHHHHCHHHHHHH
ELNPPLVEFHYKDDSLHDPLITPEDITYTGLLTSGELRWLIEATIKINNVLSELFSGAGL
HCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEECCCEEEEEEEHHHHHHHHHHHHCCCCE
KLVDFKVEYGRNRNGRLILGDELTGDSIRVLDDKGNHLDKEVFRKTGDIEALRTAYIELC
EEEEEEEECCCCCCCCEEECCCCCCCEEEEEECCCCCCCHHHHHHCCCHHHHHHHHHHHH
NRLGVNVGDVVHG
HHHCCCHHHHCCC
>Mature Secondary Structure
MVSSSINVVDMELKLLYEGKTKRVYSKTPELLVLEFKDDVTAMDGLVKKTMPGKGVINAR
CCCCCCEEEEEEEEEEECCCCCHHHCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCHHHH
LSAYLFKHIEKQGIETHLVEYNGDRFLTVRRLEMLPIEVIVRNYAYGSLLKRMPLYKKMQ
HHHHHHHHHHHCCCEEEEEEECCCEEEEEEHHHHCHHHHHHHHHHHHHHHHHCHHHHHHH
ELNPPLVEFHYKDDSLHDPLITPEDITYTGLLTSGELRWLIEATIKINNVLSELFSGAGL
HCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEECCCEEEEEEEHHHHHHHHHHHHCCCCE
KLVDFKVEYGRNRNGRLILGDELTGDSIRVLDDKGNHLDKEVFRKTGDIEALRTAYIELC
EEEEEEEECCCCCCCCEEECCCCCCCEEEEEECCCCCCCHHHHHHCCCHHHHHHHHHHHH
NRLGVNVGDVVHG
HHHCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA